LOCUS       sequence-176553-         688 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses Cas9 and contains guide RNA expression cassette with 
            BclI-SwaI cloning sites for guide sequence cloning; Contains URA3 
            marker for yeast transformation.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 688)
  AUTHORS   Laughery MF, Hunter T, Brown A, Hoopes J, Ostbye T, Shumaker T, 
            Wyrick JJ
  TITLE     New vectors for simple and streamlined CRISPR-Cas9 genome editing in
            Saccharomyces cerevisiae.
  JOURNAL   Yeast. 2015 Dec;32(12):711-20. doi: 10.1002/yea.3098. Epub 2015 Sep 
            21.
  PUBMED    26305040
REFERENCE   2  (bases 1 to 688)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1002/yea.3098"; 
            journalName: "Yeast"; date: "2015-12"; volume: "32"; issue: "12"; 
            pages: "711-20"
COMMENT     Sequence Label: pML104
FEATURES             Location/Qualifiers
     source          1..688
                     /mol_type="genomic DNA"
                     /organism="unspecified"
ORIGIN
        1 agcccgcatt cctgtcaggc gaacaaaaga aggcaattgt ggaccttctg ttcaagacca
       61 atagaaaggt gaccgtgaag cagctgaagg aggactattt caagaaaatt gaatgcttcg
      121 actctgtgga gattagcggg gtcgaagatc ggttcaacgc aagcctgggt acctaccatg
      181 atctgcttaa gatcatcaag gacaaggatt ttctggacaa tgaggagaaa gaggacatcc
      241 ttgaggacat tgtcctgact ctcactctgt tcgaggaccg ggaaatgatc gaggagaggc
      301 ttaagaccta cgcccatctg ttcgacgata aagtgatgaa gcaacttaaa cggagaagat
      361 ataccggatg gggacgcctt agccgcaaac tcatcaacgg aatccgggac aaacagagcg
      421 gaaagaccat tcttgatttc cttaagagcg acggattcgc taatcgcaac ttcatgcaac
      481 ttatccatga tgattccctg acctttaagg aggacatcca gaaggcccaa gtgtctggac
      541 aaggtgactc actgcacgag catatcgcaa atctggctgg ttcacccgct attaagaagg
      601 gtattctcca gaccgtgaaa gtcgtggacg agctggtcaa ggtgatgggt cgccataaac
      661 cagagaacat tgtcatcgag atggccag
//
