LOCUS       sequence-176557-       11987 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses catalytically-active tagRFP-cGAS after lentiviral 
            transduction. Can be used to monitor nuclear envelope rupture 
            events..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11987)
  AUTHORS   Raab M, Gentili M, de Belly H, Thiam HR, Vargas P, Jimenez AJ, 
            Lautenschlaeger F, Voituriez R, Lennon-Dumenil AM, Manel N, Piel M
  TITLE     ESCRT III repairs nuclear envelope ruptures during cell migration to
            limit DNA damage and cell death.
  JOURNAL   Science. 2016 Apr 15;352(6283):359-62. doi: 10.1126/science.aad7611.
            Epub 2016 Mar 24.
  PUBMED    27013426
REFERENCE   2  (bases 1 to 11987)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1126/science.aad7611"; journalName: "Science"; date: 
            "2016-04-15- 15"; volume: "352"; issue: "6283"; pages: "359-62"
COMMENT     Sequence Label: pTRIP-CMV-tagRFP-FLAG-cGAS
FEATURES             Location/Qualifiers
     source          1..11987
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     intron          43..108
                     /label=small t intron
                     /note="SV40 (simian virus 40) small t antigen intron"
     CDS             238..258
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     polyA_signal    683..817
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(720..739)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     774..793
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     LTR             985..1618
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     misc_feature    1665..1790
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    2283..2516
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             2701..2745
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    3038..3155
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     enhancer        3254..3557
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        3558..3761
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     3712..3732
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     regulatory      3807..3816
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             3813..4523
                     /codon_start=1
                     /product="monomeric derivative of red fluorescent protein 
                     from Entacmaea quadricolor (Merzlyak et al., 2007)"
                     /label=TagRFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELIKENMHMKLYMEGTVNNHHFKCTSEGEGKPYEGTQTM
                     RIKVVEGGPLPFAFDILATSFMYGSRTFINHTQGIPDFFKQSFPEGFTWERVTTYEDGG
                     VLTATQDTSLQDGCLIYNVKIRGVNFPSNGPVMQKKTLGWEANTEMLYPADGGLEGRTD
                     MALKLVGGGHLICNFKTTYRSKKPAKNLKMPGVYYVDHRLERIKEADKETYVEQHEVAV
                     ARYCDLPSKLGHKLN"
     CDS             4530..4553
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     LTR             6391..6571
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     primer_bind     complement(8099..8118)
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
     primer_bind     complement(8332..8351)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     8451..8473
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(8511..8529)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        8597..8701
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(8702..8770,8771..9562)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(8920..8939)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      9733..10321
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     10222..10241
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     10475..10492
                     /label=L4440
                     /note="L4440 vector, forward primer"
     primer_bind     complement(10562..10582)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        10567..10896
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      10747..10882
                     /label=SV40 ori
                     /note="SV40 origin of replication"
ORIGIN
        1 acataattgg acaaactacc tacagagatt taaagctcta aggtaaatat aaaattttta
       61 agtgtataat gtgttaaact actgattcta attgtttgtg tattttagat tccaacctat
      121 ggaactgatg aatgggagca gtggtggaat gcctttaatg aggaaaacct gttttgctca
      181 gaagaaatgc catctagtga tgatgaggct actgctgact ctcaacattc tactcctcca
      241 aaaaagaaga gaaaggtaga agaccccaag gactttcctt cagaattgct aagttttttg
      301 agtcatgctg tgtttagtaa tagaactctt gcttgctttg ctatttacac cacaaaggaa
      361 aaagctgcac tgctatacaa gaaaattatg gaaaaatatt ctgtaacctt tataagtagg
      421 cataacagtt ataatcataa catactgttt tttcttactc cacacaggca tagagtgtct
      481 gctattaata actatgctca aaaattgtgt acctttagct ttttaatttg taaaggggtt
      541 aataaggaat atttgatgta tagtgccttg actagagatc ataatcagcc ataccacatt
      601 tgtagaggtt ttacttgctt taaaaaacct cccacacctc cccctgaacc tgaaacataa
      661 aatgaatgca attgttgttg ttaacttgtt tattgcagct tataatggtt acaaataaag
      721 caatagcatc acaaatttca caaataaagc atttttttca ctgcattcta gttgtggttt
      781 gtccaaactc atcaatgtat cttatcatgt ctggatcaac tggataactc aagctaacca
      841 aaatcatccc aaacttccca ccccataccc tattaccact gccaattacc tgtggtttca
      901 tttactctaa acctgtgatt cctctgaatt attttcattt taaagaaatt gtatttgtta
      961 aatatgtact acaaacttag tagttggaag ggctaattca ctcccaaaga agacaagata
     1021 tccttgatct gtggatctac cacacacaag gctacttccc tgattagcag aactacacac
     1081 cagggccagg ggtcagatat ccactgacct ttggatggtg ctacaagcta gtaccagttg
     1141 agccagataa ggtagaagag gccaataaag gagagaacac cagcttgtta caccctgtga
     1201 gcctgcatgg gatggatgac ccggagagag aagtgttaga gtggaggttt gacagccgcc
     1261 tagcatttca tcacgtggcc cgagagctgc atccggagta cttcaagaac tgctgatatc
     1321 gagcttgcta caagggactt tccgctgggg actttccagg gaggcgtggc ctgggcggga
     1381 ctggggagtg gcgagccctc agatcctgca tataagcagc tgctttttgc ctgtactggg
     1441 tctctctggt tagaccagat ctgagcctgg gagctctctg gctaactagg gaacccactg
     1501 cttaagcctc aataaagctt gccttgagtg cttcaagtag tgtgtgcccg tctgttgtgt
     1561 gactctggta actagagatc cctcagaccc ttttagtcag tgtggaaaat ctctagcagt
     1621 ggcgcccgaa cagggacttg aaagcgaaag ggaaaccaga ggagctctct cgacgcagga
     1681 ctcggcttgc tgaagcgcgc acggcaagag gcgaggggcg gcgactggtg agtacgccaa
     1741 aaattttgac tagcggaggc tagaaggaga gagatgggtg cgagagcgtc agtattaagc
     1801 gggggagaat tagatcgcga tgggaaaaaa ttcggttaag gccaggggga aagaaaaaat
     1861 ataaattaaa acatatagta tgggcaagca gggagctaga acgattcgca gttaatcctg
     1921 gcctgttaga aacatcagaa ggctgtagac aaatactggg acagctacaa ccatcccttc
     1981 agacaggatc agaagaactt agatcattat ataatacagt agcaaccctc tattgtgtgc
     2041 atcaaaggat agagataaaa gacaccaagg aagctttaga caagatagag gaagagcaaa
     2101 acaaaagtaa gaccaccgca cagcaagcgg ccgctgatct tcagacctgg aggaggagat
     2161 atgagggaca attggagaag tgaattatat aaatataaag tagtaaaaat tgaaccatta
     2221 ggagtagcac ccaccaaggc aaagagaaga gtggtgcaga gagaaaaaag agcagtggga
     2281 ataggagctt tgttccttgg gttcttggga gcagcaggaa gcactatggg cgcagcgtca
     2341 atgacgctga cggtacaggc cagacaatta ttgtctggta tagtgcagca gcagaacaat
     2401 ttgctgaggg ctattgaggc gcaacagcat ctgttgcaac tcacagtctg gggcatcaag
     2461 cagctccagg caagaatcct ggctgtggaa agatacctaa aggatcaaca gctcctgggg
     2521 atttggggtt gctctggaaa actcatttgc accactgctg tgccttggaa tgctagttgg
     2581 agtaataaat ctctggaaca gatttggaat cacacgacct ggatggagtg ggacagagaa
     2641 attaacaatt acacaagctt aatacactcc ttaattgaag aatcgcaaaa ccagcaagaa
     2701 aagaatgaac aagaattatt ggaattagat aaatgggcaa gtttgtggaa ttggtttaac
     2761 ataacaaatt ggctgtggta tataaaatta ttcataatga tagtaggagg cttggtaggt
     2821 ttaagaatag tttttgctgt actttctata gtgaatagag ttaggcaggg atattcacca
     2881 ttatcgtttc agacccacct cccaaccccg aggggacccg acaggcccga aggaatagaa
     2941 gaagaaggtg gagagagaga cagagacaga tccattcgat tagtgaacgg atctcgacgg
     3001 tatcgccgaa ttcacaaatg gcagtattca tccacaattt taaaagaaaa ggggggattg
     3061 gggggtacag tgcaggggaa agaatagtag acataatagc aacagacata caaactaaag
     3121 aattacaaaa acaaattaca aaaattcaaa attttcgggt ttattacagg gacagcagag
     3181 atccactttg gctgatacgc gtatagtaat caattacggg gtcattagtt catagcccat
     3241 atatggagtt ccgcgttaca taacttacgg taaatggccc gcctggctga ccgcccaacg
     3301 acccccgccc attgacgtca ataatgacgt atgttcccat agtaacgcca atagggactt
     3361 tccattgacg tcaatgggtg gagtatttac ggtaaactgc ccacttggca gtacatcaag
     3421 tgtatcatat gccaagtacg ccccctattg acgtcaatga cggtaaatgg cccgcctggc
     3481 attatgccca gtacatgacc ttatgggact ttcctacttg gcagtacatc tacgtattag
     3541 tcatcgctat taccatggtg atgcggtttt ggcagtacat caatgggcgt ggatagcggt
     3601 ttgactcacg gggatttcca agtctccacc ccattgacgt caatgggagt ttgttttggc
     3661 accaaaatca acgggacttt ccaaaatgtc gtaacaactc cgccccattg acgcaaatgg
     3721 gcggtaggcg tgtacggtgg gaggtctata taagcagagc tggtttagtg aaccgtcaga
     3781 tcccgcgggc tagcggatct gtcgacgcca ccatggtgtc taagggcgaa gagctgatta
     3841 aggagaacat gcacatgaag ctgtacatgg agggcaccgt gaacaaccac cacttcaagt
     3901 gcacatccga gggcgaaggc aagccctacg agggcaccca gaccatgaga atcaaggtgg
     3961 tcgagggcgg ccctctcccc ttcgccttcg acatcctggc taccagcttc atgtacggca
     4021 gcagaacctt catcaaccac acccagggca tccccgactt ctttaagcag tccttccctg
     4081 agggcttcac atgggagaga gtcaccacat acgaagacgg gggcgtgctg accgctaccc
     4141 aggacaccag cctccaggac ggctgcctca tctacaacgt caagatcaga ggggtgaact
     4201 tcccatccaa cggccctgtg atgcagaaga aaacactcgg ctgggaggcc aacaccgaga
     4261 tgctgtaccc cgctgacggc ggcctggaag gcagaaccga catggccctg aagctcgtgg
     4321 gcgggggcca cctgatctgc aacttcaaga ccacatacag atccaagaaa cccgctaaga
     4381 acctcaagat gcccggcgtc tactatgtgg accacagact ggaaagaatc aaggaggccg
     4441 acaaagagac ctacgtcgag cagcacgagg tggctgtggc cagatactgc gacctcccta
     4501 gcaaactggg gcacaaactt aatactagtg actacaaaga cgatgacgac aagatgcagc
     4561 cttggcacgg aaaggccatg cagagagctt ccgaggccgg agccactgcc cccaaggctt
     4621 ccgcacggaa tgccaggggc gccccgatgg atcccaacga gtctccggct gcccccgagg
     4681 ccgccctgcc taaggcggga aagttcggcc ccgccaggaa gtcgggatcc cggcagaaaa
     4741 agagcgcccc ggacacccag gagaggccgc ccgtccgcgc aactggggcc cgcgccaaaa
     4801 aggcccctca gcgcgcccag gacacgcagc cgtctgacgc caccagcgcc cctggggcag
     4861 aggggctgga gcctcctgcg gctcgggagc cggctctttc cagggctggt tcttgccgcc
     4921 agaggggcgc gcgctgctcc acgaagccaa gacccccgcc cgggccctgg gacgtgccca
     4981 gccccggcct gccggtctcg gcccccattc tcgtacggag ggatgcggcg cctggggcct
     5041 cgaagctccg ggcggttttg gagaagttga agctcagccg cgatgatatc tccacggcgg
     5101 cggggatggt gaaaggggtt gtggaccacc tgctgctcag actgaagtgc gactccgcgt
     5161 tcagaggcgt cgggctgctg aacaccggga gctactatga gcacgtgaag atttctgcac
     5221 ctaatgaatt tgatgtcatg tttaaactgg aagtccccag aattcaacta gaagaatatt
     5281 ccaacactcg tgcatattac tttgtgaaat ttaaaagaaa tccgaaagaa aatcatctga
     5341 gtcagttttt agaaggtgaa atattatcag cttctaagat gctgtcaaag tttaggaaaa
     5401 tcattaagga agaaattaac gacattaaag atacagatgt catcatgaag aggaaaagag
     5461 gagggagccc tgctgtaaca cttcttatta gtgaaaaaat atctgtggat ataaccctgg
     5521 ctttggaatc aaaaagtagc tggcctgcta gcacccaaga aggcctgcgc attcaaaact
     5581 ggctttcagc aaaagttagg aagcaactac gactaaagcc attttacctt gtacccaagc
     5641 atgcaaagga aggaaatggt ttccaagaag aaacatggcg gctatccttc tctcacatcg
     5701 aaaaggaaat tttgaacaat catggaaaat ctaaaacgtg ctgtgaaaac aaagaagaga
     5761 aatgttgcag gaaagattgt ttaaaactaa tgaaatacct tttagaacag ctgaaagaaa
     5821 ggtttaaaga caaaaaacat ctggataaat tctcttctta tcatgtgaaa actgccttct
     5881 ttcacgtatg tacccagaac cctcaagaca gtcagtggga ccgcaaagac ctgggcctct
     5941 gctttgataa ctgcgtgaca tactttcttc agtgcctcag gacagaaaaa cttgagaatt
     6001 attttattcc tgaattcaat ctattctcta gcaacttaat tgacaaaaga agtaaagaat
     6061 ttctgacaaa gcaaattgaa tatgaaagaa acaatgagtt tccagttttt gatgaatttt
     6121 gactcgagac ctagaaaaac atggagcaat cacaagtagc aatacagcag ctaccaatgc
     6181 tgattgtgcc tggctagaag cacaagagga ggaggaggtg ggttttccag tcacacctca
     6241 ggtaccttta agaccaatga cttacaaggc agctgtagat cttagccact ttttaaaaga
     6301 aaagggggga ctggaagggc taattcactc ccaacgaaga caaaatcgtc gagagatgct
     6361 gcatataagc agctgctttt tgcttgtact gggtctctct ggttagacca gatctgagcc
     6421 tgggagctct ctggctaact agggaaccca ctgcttaagc ctcaataaag cttgccttga
     6481 gtgcttcaag tagtgtgtgc ccgtctgttg tgtgactctg gtaactagag atccctcaga
     6541 cccttttagt cagtgtggaa aatctctagc agtagtagtt catgtcatct tattattcag
     6601 tatttataac ttgcaaagaa atgaatatca gagagtgaga ggccttgaca ttataataga
     6661 tttagcagga attgaactag gagtggagca cacaggcaaa gctgcagaag tacttggaag
     6721 aagccaccag agatactcac gattctgcac atacctggct aatcccagat cctaaggatt
     6781 acattaagtt tactaacatt tatataatga tttatagttt aaagtataaa cttatctaat
     6841 ttactattct gacagatatt aattaatcct caaatatcat aagagatgat tactattatc
     6901 cccatttaac acaagaggaa actgagaggg aaagatgttg aagtaatttt cccacaatta
     6961 cagcatccgt tagttacgac tctatgatct tctgacacaa attccattta ctcctcaccc
     7021 tatgactcag tcgaatatat caaagttatg gacattatgc taagtaacaa attacccttt
     7081 tatatagtaa atactgagta gattgagaga agaaattgtt tgcaaacctg aatagcttca
     7141 agaagaagag aagtgaggat aagaataaca gttgtcattt aacaagtttt aacaagtaac
     7201 ttggttagaa agggattcaa atgcataaag caagggataa atttttctgg caacaagact
     7261 atacaatata accttaaata tgacttcaaa taattgttgg aacttgataa aactaattaa
     7321 atattattga agattatcaa tattataaat gtaatttact tttaaaaagg gaacatagaa
     7381 atgtgtatca ttagagtaga aaacaatcct tattatcaca atttgtcaaa acaagtttgt
     7441 tattaacaca agtagaatac tgcattcaat taagttgact gcagattttg tgttttgtta
     7501 aaattagaaa gagataacaa caatttgaat tattgaaagt aacatgtaaa tagttctaca
     7561 tacgttcttt tgacatcttg ttcaatcatt gatcgaagtt ctttatcttg gaagaatttg
     7621 ttccaaagac tctgaaataa ggaaaacaat ctattatata gtctcacacc tttgttttac
     7681 ttttagtgat ttcaatttaa taatgtaaat ggttaaaatt tattcttctc tgagatcatt
     7741 tcacattgca gatagaaaac ctgagactgg ggtaattttt attaaaatct aatttaatct
     7801 cagaaacaca tctttattct aacatcaatt tttccagttt gatattatca tataaagtca
     7861 gccttcctca tctgcaggtt ccacaacaaa aatccaacca actgtggatc aaaaatattg
     7921 ggaaaaaatt aaaaatagca atacaacaat aaaaaaatac aaatcagaaa aacagcacag
     7981 tataacaact ttatttagca tttacaatct attaggtatt ataagtaatc tagccagatc
     8041 ctctacgccg gacgcatcgt ggccggcatc accggcgcca caggtgcggt tgctggcgcc
     8101 tatatcgccg acatcaccga tggggaagat cgggctcgcc acttcgggct catgagcgct
     8161 tgtttcggcg tgggtatggt ggcaggcccc gtggccgggg gactgttggg cgccatctcc
     8221 ttgcatgcac cattccttgc ggcggcggtg ctcaacggcc tcaacctact actgggctgc
     8281 ttcctaatgc aggagtcgca taagggagag cgtcgaatgg tgcactctca gtacaatctg
     8341 ctctgatgcc gcatagttaa gccagccccg acacccgcca acacccgctg acgcgccctg
     8401 acgggcttgt ctgctcccgg catccgctta cagacaagct gtgaccgtct ccgggagctg
     8461 catgtgtcag aggttttcac cgtcatcacc gaaacgcgcg agacgaaagg gcctcgtgat
     8521 acgcctattt ttataggtta atgtcatgat aataatggtt tcttagacgt caggtggcac
     8581 ttttcgggga aatgtgcgcg gaacccctat ttgtttattt ttctaaatac attcaaatat
     8641 gtatccgctc atgagacaat aaccctgata aatgcttcaa taatattgaa aaaggaagag
     8701 tatgagtatt caacatttcc gtgtcgccct tattcccttt tttgcggcat tttgccttcc
     8761 tgtttttgct cacccagaaa cgctggtgaa agtaaaagat gctgaagatc agttgggtgc
     8821 acgagtgggt tacatcgaac tggatctcaa cagcggtaag atccttgaga gttttcgccc
     8881 cgaagaacgt tttccaatga tgagcacttt taaagttctg ctatgtggcg cggtattatc
     8941 ccgtattgac gccgggcaag agcaactcgg tcgccgcata cactattctc agaatgactt
     9001 ggttgagtac tcaccagtca cagaaaagca tcttacggat ggcatgacag taagagaatt
     9061 atgcagtgct gccataacca tgagtgataa cactgcggcc aacttacttc tgacaacgat
     9121 cggaggaccg aaggagctaa ccgctttttt gcacaacatg ggggatcatg taactcgcct
     9181 tgatcgttgg gaaccggagc tgaatgaagc cataccaaac gacgagcgtg acaccacgat
     9241 gcctgtagca atggcaacaa cgttgcgcaa actattaact ggcgaactac ttactctagc
     9301 ttcccggcaa caattaatag actggatgga ggcggataaa gttgcaggac cacttctgcg
     9361 ctcggccctt ccggctggct ggtttattgc tgataaatct ggagccggtg agcgtgggtc
     9421 tcgcggtatc attgcagcac tggggccaga tggtaagccc tcccgtatcg tagttatcta
     9481 cacgacgggg agtcaggcaa ctatggatga acgaaataga cagatcgctg agataggtgc
     9541 ctcactgatt aagcattggt aactgtcaga ccaagtttac tcatatatac tttagattga
     9601 tttaaaactt catttttaat ttaaaaggat ctaggtgaag atcctttttg ataatctcat
     9661 gaccaaaatc ccttaacgtg agttttcgtt ccactgagcg tcagaccccg tagaaaagat
     9721 caaaggatct tcttgagatc ctttttttct gcgcgtaatc tgctgcttgc aaacaaaaaa
     9781 accaccgcta ccagcggtgg tttgtttgcc ggatcaagag ctaccaactc tttttccgaa
     9841 ggtaactggc ttcagcagag cgcagatacc aaatactgtt cttctagtgt agccgtagtt
     9901 aggccaccac ttcaagaact ctgtagcacc gcctacatac ctcgctctgc taatcctgtt
     9961 accagtggct gctgccagtg gcgataagtc gtgtcttacc gggttggact caagacgata
    10021 gttaccggat aaggcgcagc ggtcgggctg aacggggggt tcgtgcacac agcccagctt
    10081 ggagcgaacg acctacaccg aactgagata cctacagcgt gagctatgag aaagcgccac
    10141 gcttcccgaa gggagaaagg cggacaggta tccggtaagc ggcagggtcg gaacaggaga
    10201 gcgcacgagg gagcttccag ggggaaacgc ctggtatctt tatagtcctg tcgggtttcg
    10261 ccacctctga cttgagcgtc gatttttgtg atgctcgtca ggggggcgga gcctatggaa
    10321 aaacgccagc aacgcggcct ttttacggtt cctggccttt tgctggcctt ttgctcacat
    10381 gttctttcct gcgttatccc ctgattctgt ggataaccgt attaccgcct ttgagtgagc
    10441 tgataccgct cgccgcagcc gaacgaccga gcgcagcgag tcagtgagcg aggaagcgga
    10501 agagcgccca atacgcaaac cgcctctccc cgcgcgttgg ccgattcatt aatgcagctg
    10561 tggaatgtgt gtcagttagg gtgtggaaag tccccaggct ccccagcagg cagaagtatg
    10621 caaagcatgc atctcaatta gtcagcaacc aggtgtggaa agtccccagg ctccccagca
    10681 ggcagaagta tgcaaagcat gcatctcaat tagtcagcaa ccatagtccc gcccctaact
    10741 ccgcccatcc cgcccctaac tccgcccagt tccgcccatt ctccgcccca tggctgacta
    10801 atttttttta tttatgcaga ggccgaggcc gcctcggcct ctgagctatt ccagaagtag
    10861 tgaggaggct tttttggagg cctaggcttt tgcaaaaagc ttggacacaa gacaggcttg
    10921 cgagatatgt ttgagaatac cactttatcc cgcgtcaggg agaggcagtg cgtaaaaaga
    10981 cgcggactca tgtgaaatac tggtttttag tgcgccagat ctctataatc tcgcgcaacc
    11041 tattttcccc tcgaacactt tttaagccgt agataaacag gctgggacac ttcacatgag
    11101 cgaaaaatac atcgtcacct gggacatgtt gcagatccat gcacgtaaac tcgcaagccg
    11161 actgatgcct tctgaacaat ggaaaggcat tattgccgta agccgtggcg gtctgtaccg
    11221 ggtgcgttac tggcgcgtga actgggtatt cgtcatgtcg ataccgtttg tatttccagc
    11281 tacgatcacg acaaccagcg cgagcttaaa gtgctgaaac gcgcagaagg cgatggcgaa
    11341 ggcttcatcg ttattgatga cctggtggat accggtggta ctgcggttgc gattcgtgaa
    11401 atgtatccaa aagcgcactt tgtcaccatc ttcgcaaaac cggctggtcg tccgctggtt
    11461 gatgactatg ttgttgatat cccgcaagat acctggattg aacagccgtg ggatatgggc
    11521 gtcgtattcg tcccgccaat ctccggtcgc taatcttttc aacgcctggc actgccgggc
    11581 gttgttcttt ttaacttcag gcgggttaca atagtttcca gtaagtattc tggaggctgc
    11641 atccatgaca caggcaaacc tgagcgaaac cctgttcaaa ccccgcttta aacatcctga
    11701 aacctcgacg ctagtccgcc gctttaatca cggcgcacaa ccgcctgtgc agtcggccct
    11761 tgatggtaaa accatccctc actggtatcg catgattaac cgtctgatgt ggatctggcg
    11821 cggcattgac ccacgcgaaa tcctcgacgt ccaggcacgt attgtgatga gcgatgccga
    11881 acgtaccgac gatgatttat acgatacggt gattggctac cgtggcggca actggattta
    11941 tgagtgggcc ccggatcttt gtgaaggaac cttacttctg tggtgtg
//
