LOCUS       sequence-176563-        3794 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Can be used for in situ hybridisation or other purposes.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3794)
  AUTHORS   Hall TE, Martel N, Lo HP, Xiong Z, Parton RG
  TITLE     A plasmid library of full-length zebrafish rab proteins for in vivo 
            cell biology.
  JOURNAL   Cell Logist. 2017 Mar 1;7(1):e1301151. doi: 
            10.1080/21592799.2017.1301151. eCollection 2017.
  PUBMED    28396820
REFERENCE   2  (bases 1 to 3794)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell 
            Logist."; date: "2017-03-1"; volume: "7(1):e1301151. doi"; pages: " 
            10.1080/21592799.2017.1301151. eCollection 2017"
COMMENT     Sequence Label: Rab38a cds in pGem-T Easy
FEATURES             Location/Qualifiers
     source          1..3794
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(26..43)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(197..785)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(277..296)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(956..1747,1748..1816))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     1579..1598
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(1817..1921)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      complement(1999..2454)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(2136..2157)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     2348..2367
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     2580..2602
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     2594..2611
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     2595..2611
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     2618..2637
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        2618..2636
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     regulatory      2667..2676
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     regulatory      2671..2680
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     promoter        complement(3553..3571)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(3554..3571)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     complement(3589..3605)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(3589..3605)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(3602..3624)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    3613..3629
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(3637..3643,3644..3661,3662..3667))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    3682..3703
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
ORIGIN
        1 tgcgtattgg gcgctcttcc gcttcctcgc tcactgactc gctgcgctcg gtcgttcggc
       61 tgcggcgagc ggtatcagct cactcaaagg cggtaatacg gttatccaca gaatcagggg
      121 ataacgcagg aaagaacatg tgagcaaaag gccagcaaaa ggccaggaac cgtaaaaagg
      181 ccgcgttgct ggcgtttttc cataggctcc gcccccctga cgagcatcac aaaaatcgac
      241 gctcaagtca gaggtggcga aacccgacag gactataaag ataccaggcg tttccccctg
      301 gaagctccct cgtgcgctct cctgttccga ccctgccgct taccggatac ctgtccgcct
      361 ttctcccttc gggaagcgtg gcgctttctc atagctcacg ctgtaggtat ctcagttcgg
      421 tgtaggtcgt tcgctccaag ctgggctgtg tgcacgaacc ccccgttcag cccgaccgct
      481 gcgccttatc cggtaactat cgtcttgagt ccaacccggt aagacacgac ttatcgccac
      541 tggcagcagc cactggtaac aggattagca gagcgaggta tgtaggcggt gctacagagt
      601 tcttgaagtg gtggcctaac tacggctaca ctagaagaac agtatttggt atctgcgctc
      661 tgctgaagcc agttaccttc ggaaaaagag ttggtagctc ttgatccggc aaacaaacca
      721 ccgctggtag cggtggtttt tttgtttgca agcagcagat tacgcgcaga aaaaaaggat
      781 ctcaagaaga tcctttgatc ttttctacgg ggtctgacgc tcagtggaac gaaaactcac
      841 gttaagggat tttggtcatg agattatcaa aaaggatctt cacctagatc cttttaaatt
      901 aaaaatgaag ttttaaatca atctaaagta tatatgagta aacttggtct gacagttacc
      961 aatgcttaat cagtgaggca cctatctcag cgatctgtct atttcgttca tccatagttg
     1021 cctgactccc cgtcgtgtag ataactacga tacgggaggg cttaccatct ggccccagtg
     1081 ctgcaatgat accgcgagac ccacgctcac cggctccaga tttatcagca ataaaccagc
     1141 cagccggaag ggccgagcgc agaagtggtc ctgcaacttt atccgcctcc atccagtcta
     1201 ttaattgttg ccgggaagct agagtaagta gttcgccagt taatagtttg cgcaacgttg
     1261 ttgccattgc tacaggcatc gtggtgtcac gctcgtcgtt tggtatggct tcattcagct
     1321 ccggttccca acgatcaagg cgagttacat gatcccccat gttgtgcaaa aaagcggtta
     1381 gctccttcgg tcctccgatc gttgtcagaa gtaagttggc cgcagtgtta tcactcatgg
     1441 ttatggcagc actgcataat tctcttactg tcatgccatc cgtaagatgc ttttctgtga
     1501 ctggtgagta ctcaaccaag tcattctgag aatagtgtat gcggcgaccg agttgctctt
     1561 gcccggcgtc aatacgggat aataccgcgc cacatagcag aactttaaaa gtgctcatca
     1621 ttggaaaacg ttcttcgggg cgaaaactct caaggatctt accgctgttg agatccagtt
     1681 cgatgtaacc cactcgtgca cccaactgat cttcagcatc ttttactttc accagcgttt
     1741 ctgggtgagc aaaaacagga aggcaaaatg ccgcaaaaaa gggaataagg gcgacacgga
     1801 aatgttgaat actcatactc ttcctttttc aatattattg aagcatttat cagggttatt
     1861 gtctcatgag cggatacata tttgaatgta tttagaaaaa taaacaaata ggggttccgc
     1921 gcacatttcc ccgaaaagtg ccacctgatg cggtgtgaaa taccgcacag atgcgtaagg
     1981 agaaaatacc gcatcaggaa attgtaagcg ttaatatttt gttaaaattc gcgttaaatt
     2041 tttgttaaat cagctcattt tttaaccaat aggccgaaat cggcaaaatc ccttataaat
     2101 caaaagaata gaccgagata gggttgagtg ttgttccagt ttggaacaag agtccactat
     2161 taaagaacgt ggactccaac gtcaaagggc gaaaaaccgt ctatcagggc gatggcccac
     2221 tacgtgaacc atcaccctaa tcaagttttt tggggtcgag gtgccgtaaa gcactaaatc
     2281 ggaaccctaa agggagcccc cgatttagag cttgacgggg aaagccggcg aacgtggcga
     2341 gaaaggaagg gaagaaagcg aaaggagcgg gcgctagggc gctggcaagt gtagcggtca
     2401 cgctgcgcgt aaccaccaca cccgccgcgc ttaatgcgcc gctacagggc gcgtccattc
     2461 gccattcagg ctgcgcaact gttgggaagg gcgatcggtg cgggcctctt cgctattacg
     2521 ccagctggcg aaagggggat gtgctgcaag gcgattaagt tgggtaacgc cagggttttc
     2581 ccagtcacga cgttgtaaaa cgacggccag tgaattgtaa tacgactcac tatagggcga
     2641 attgggcccg acgtcgcatg ctcccggccg ccatggcggc cgcgggaatt cgattctggt
     2701 ctcatgctcg actggcgagt cacaagttgt gctttttccc tccgcggggc gtttctgttt
     2761 ctcccagagt ctctcccagc ggatcacacg cgtccatgtt cgcctctgtc tcacacagcc
     2821 atgcagaacc atcacctgaa ggaacatctc tataaaatcc tggtgatcgg ggacctggga
     2881 gtggggaaaa ccagcattat caagcgctac gtgcaccaga acttctcacc aaactaccgt
     2941 gccaccatcg gcgtggattt cgcgctcaaa gttctgaatc tcgaccagga gaccgttagg
     3001 ctgcagctct gggacatcgc aggtcaggag cgtttcggga acatgacgcg ggtctattac
     3061 agagaagcaa tgggagccat cattgttttt gacgtcacgc ggccgtcgac gtttgaggcg
     3121 gtgacgaagt ggaaggagga cttggatgca aagctgagtt tatccaacgg caaacaagtg
     3181 gcggcggtgc tgctggccaa caaatgtgat cagggccgag acgtgctcac caacaacggc
     3241 atcaagatgg agcagttctg ccaggagaac ggcttcgtgg gatggttcga aacttcagcc
     3301 aaggagaata ttaatataga tgaagccgca cactgtctgg tgaagcacat tatagctaat
     3361 gagaacgatc tgctgcagtc ggaggttgca gacactattt caccgcagca ggagtccagt
     3421 cgtggaggaa cctgctcggc ctgtttcagg tcctgaagac gctccgtttc tcaatcacta
     3481 gtgaattcgc ggccgcctgc aggtcgacca tatgggagag ctcccaacgc gttggatgca
     3541 tagcttgagt attctatagt gtcacctaaa tagcttggcg taatcatggt catagctgtt
     3601 tcctgtgtga aattgttatc cgctcacaat tccacacaac atacgagccg gaagcataaa
     3661 gtgtaaagcc tggggtgcct aatgagtgag ctaactcaca ttaattgcgt tgcgctcact
     3721 gcccgctttc cagtcgggaa acctgtcgtg ccagctgcat taatgaatcg gccaacgcgc
     3781 ggggagaggc ggtt
//
