LOCUS       sequence-176564-        3696 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Can be used for in situ hybridisation or other purposes.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3696)
  AUTHORS   Hall TE, Martel N, Lo HP, Xiong Z, Parton RG
  TITLE     A plasmid library of full-length zebrafish rab proteins for in vivo 
            cell biology.
  JOURNAL   Cell Logist. 2017 Mar 1;7(1):e1301151. doi: 
            10.1080/21592799.2017.1301151. eCollection 2017.
  PUBMED    28396820
REFERENCE   2  (bases 1 to 3696)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell 
            Logist."; date: "2017-03-1"; volume: "7(1):e1301151. doi"; pages: " 
            10.1080/21592799.2017.1301151. eCollection 2017"
COMMENT     Sequence Label: Rab42b cds in pGem-T Easy
FEATURES             Location/Qualifiers
     source          1..3696
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(8..25)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(179..767)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(259..278)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(938..1729,1730..1798))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     1561..1580
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(1799..1903)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      complement(1981..2436)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(2118..2139)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     2330..2349
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     2562..2584
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     2576..2593
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     2577..2593
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     2600..2619
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        2600..2618
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     regulatory      2649..2658
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     regulatory      2653..2662
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     promoter        complement(3437..3455)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(3438..3455)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     complement(3473..3489)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(3473..3489)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(3486..3508)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    3497..3513
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(3521..3527,3528..3545,3546..3551))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    3566..3587
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
ORIGIN
        1 ccgcttcctc gctcactgac tcgctgcgct cggtcgttcg gctgcggcga gcggtatcag
       61 ctcactcaaa ggcggtaata cggttatcca cagaatcagg ggataacgca ggaaagaaca
      121 tgtgagcaaa aggccagcaa aaggccagga accgtaaaaa ggccgcgttg ctggcgtttt
      181 tccataggct ccgcccccct gacgagcatc acaaaaatcg acgctcaagt cagaggtggc
      241 gaaacccgac aggactataa agataccagg cgtttccccc tggaagctcc ctcgtgcgct
      301 ctcctgttcc gaccctgccg cttaccggat acctgtccgc ctttctccct tcgggaagcg
      361 tggcgctttc tcatagctca cgctgtaggt atctcagttc ggtgtaggtc gttcgctcca
      421 agctgggctg tgtgcacgaa ccccccgttc agcccgaccg ctgcgcctta tccggtaact
      481 atcgtcttga gtccaacccg gtaagacacg acttatcgcc actggcagca gccactggta
      541 acaggattag cagagcgagg tatgtaggcg gtgctacaga gttcttgaag tggtggccta
      601 actacggcta cactagaaga acagtatttg gtatctgcgc tctgctgaag ccagttacct
      661 tcggaaaaag agttggtagc tcttgatccg gcaaacaaac caccgctggt agcggtggtt
      721 tttttgtttg caagcagcag attacgcgca gaaaaaaagg atctcaagaa gatcctttga
      781 tcttttctac ggggtctgac gctcagtgga acgaaaactc acgttaaggg attttggtca
      841 tgagattatc aaaaaggatc ttcacctaga tccttttaaa ttaaaaatga agttttaaat
      901 caatctaaag tatatatgag taaacttggt ctgacagtta ccaatgctta atcagtgagg
      961 cacctatctc agcgatctgt ctatttcgtt catccatagt tgcctgactc cccgtcgtgt
     1021 agataactac gatacgggag ggcttaccat ctggccccag tgctgcaatg ataccgcgag
     1081 acccacgctc accggctcca gatttatcag caataaacca gccagccgga agggccgagc
     1141 gcagaagtgg tcctgcaact ttatccgcct ccatccagtc tattaattgt tgccgggaag
     1201 ctagagtaag tagttcgcca gttaatagtt tgcgcaacgt tgttgccatt gctacaggca
     1261 tcgtggtgtc acgctcgtcg tttggtatgg cttcattcag ctccggttcc caacgatcaa
     1321 ggcgagttac atgatccccc atgttgtgca aaaaagcggt tagctccttc ggtcctccga
     1381 tcgttgtcag aagtaagttg gccgcagtgt tatcactcat ggttatggca gcactgcata
     1441 attctcttac tgtcatgcca tccgtaagat gcttttctgt gactggtgag tactcaacca
     1501 agtcattctg agaatagtgt atgcggcgac cgagttgctc ttgcccggcg tcaatacggg
     1561 ataataccgc gccacatagc agaactttaa aagtgctcat cattggaaaa cgttcttcgg
     1621 ggcgaaaact ctcaaggatc ttaccgctgt tgagatccag ttcgatgtaa cccactcgtg
     1681 cacccaactg atcttcagca tcttttactt tcaccagcgt ttctgggtga gcaaaaacag
     1741 gaaggcaaaa tgccgcaaaa aagggaataa gggcgacacg gaaatgttga atactcatac
     1801 tcttcctttt tcaatattat tgaagcattt atcagggtta ttgtctcatg agcggataca
     1861 tatttgaatg tatttagaaa aataaacaaa taggggttcc gcgcacattt ccccgaaaag
     1921 tgccacctga tgcggtgtga aataccgcac agatgcgtaa ggagaaaata ccgcatcagg
     1981 aaattgtaag cgttaatatt ttgttaaaat tcgcgttaaa tttttgttaa atcagctcat
     2041 tttttaacca ataggccgaa atcggcaaaa tcccttataa atcaaaagaa tagaccgaga
     2101 tagggttgag tgttgttcca gtttggaaca agagtccact attaaagaac gtggactcca
     2161 acgtcaaagg gcgaaaaacc gtctatcagg gcgatggccc actacgtgaa ccatcaccct
     2221 aatcaagttt tttggggtcg aggtgccgta aagcactaaa tcggaaccct aaagggagcc
     2281 cccgatttag agcttgacgg ggaaagccgg cgaacgtggc gagaaaggaa gggaagaaag
     2341 cgaaaggagc gggcgctagg gcgctggcaa gtgtagcggt cacgctgcgc gtaaccacca
     2401 cacccgccgc gcttaatgcg ccgctacagg gcgcgtccat tcgccattca ggctgcgcaa
     2461 ctgttgggaa gggcgatcgg tgcgggcctc ttcgctatta cgccagctgg cgaaaggggg
     2521 atgtgctgca aggcgattaa gttgggtaac gccagggttt tcccagtcac gacgttgtaa
     2581 aacgacggcc agtgaattgt aatacgactc actatagggc gaattgggcc cgacgtcgca
     2641 tgctcccggc cgccatggcg gccgcgggaa ttcgattaag tcatggatct cacattgtgg
     2701 cagtaccagt ttcggatcat catgcttgga gactctacag ttggaaagtc gtccatgtta
     2761 aagcgctaca ctgaggactt gtttctggaa tgcatcaatc agactgtcgg agtggacttc
     2821 tatgtacact tcctagaggt ggaacctggg gtgagggtta aactgcagtt ttgggacaca
     2881 gctggtcaag agaggtttag gtctgtgact cgatcctact accgtaactc tgttggaggt
     2941 ctcctggtgt tcgatctggg caatcgcaaa tcttttgaaa atgtgcggga atggtacgcc
     3001 gaagtgtgtg aacgtgtgca tccctacact gtgctcttcg tcctggtagg acacaagagc
     3061 gaccgcgtca aaggtggaga aagagccgtg gaccgaactg aagcagaaaa gttggcgagc
     3121 cagctgggag ccccttacat cgaggcctcg gccaaaacgg gtcacaatgt gaaagaagcc
     3181 ttcgacctct tgacccggcg gatttatcag ggcttaaaaa gtggtgagat ccagttgcgc
     3241 gaagggtggg atggcgtcaa aagctcagcg cctacagccc aaaccctcca gaagttacaa
     3301 aacactgaat ctgctgagaa aaagagctgt aactgttaac tattacaaca cgtggcaatc
     3361 actagtgaat tcgcggccgc ctgcaggtcg accatatggg agagctccca acgcgttgga
     3421 tgcatagctt gagtattcta tagtgtcacc taaatagctt ggcgtaatca tggtcatagc
     3481 tgtttcctgt gtgaaattgt tatccgctca caattccaca caacatacga gccggaagca
     3541 taaagtgtaa agcctggggt gcctaatgag tgagctaact cacattaatt gcgttgcgct
     3601 cactgcccgc tttccagtcg ggaaacctgt cgtgccagct gcattaatga atcggccaac
     3661 gcgcggggag aggcggtttg cgtattgggc gctctt
//
