LOCUS       sequence-176565-        3675 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Can be used for in situ hybridisation or other purposes.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3675)
  AUTHORS   Hall TE, Martel N, Lo HP, Xiong Z, Parton RG
  TITLE     A plasmid library of full-length zebrafish rab proteins for in vivo 
            cell biology.
  JOURNAL   Cell Logist. 2017 Mar 1;7(1):e1301151. doi: 
            10.1080/21592799.2017.1301151. eCollection 2017.
  PUBMED    28396820
REFERENCE   2  (bases 1 to 3675)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell 
            Logist."; date: "2017-03-1"; volume: "7(1):e1301151. doi"; pages: " 
            10.1080/21592799.2017.1301151. eCollection 2017"
COMMENT     Sequence Label: Rab42a cds in pGem-T Easy
FEATURES             Location/Qualifiers
     source          1..3675
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     123..142
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     376..393
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    510..531
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(546..551,552..569,570..576)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    584..600
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     589..611
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     608..624
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     608..624
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     promoter        642..660
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     642..659
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     regulatory      1414..1423
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     regulatory      1418..1427
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     primer_bind     complement(1457..1476)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(1458..1476)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(1483..1500)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(1483..1499)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(1492..1514)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      1640..2095
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(1727..1746)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     1937..1958
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        2173..2277
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(2278..2346,2347..3138)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(2496..2515)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      join(3309..3675,1..222)
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
ORIGIN
        1 gaactgagat acctacagcg tgagctatga gaaagcgcca cgcttcccga agggagaaag
       61 gcggacaggt atccggtaag cggcagggtc ggaacaggag agcgcacgag ggagcttcca
      121 gggggaaacg cctggtatct ttatagtcct gtcgggtttc gccacctctg acttgagcgt
      181 cgatttttgt gatgctcgtc aggggggcgg agcctatgga aaaacgccag caacgcggcc
      241 tttttacggt tcctggcctt ttgctggcct tttgctcaca tgttctttcc tgcgttatcc
      301 cctgattctg tggataaccg tattaccgcc tttgagtgag ctgataccgc tcgccgcagc
      361 cgaacgaccg agcgcagcga gtcagtgagc gaggaagcgg aagagcgccc aatacgcaaa
      421 ccgcctctcc ccgcgcgttg gccgattcat taatgcagct ggcacgacag gtttcccgac
      481 tggaaagcgg gcagtgagcg caacgcaatt aatgtgagtt agctcactca ttaggcaccc
      541 caggctttac actttatgct tccggctcgt atgttgtgtg gaattgtgag cggataacaa
      601 tttcacacag gaaacagcta tgaccatgat tacgccaagc tatttaggtg acactataga
      661 atactcaagc tatgcatcca acgcgttggg agctctccca tatggtcgac ctgcaggcgg
      721 ccgcgaattc actagtgatt gccatggata ctttatggca gtaccagttc aggatcattt
      781 tactcgggga ctcgacggtg ggcaaatcat ctttgctcaa gaggttcacg gatggcatat
      841 acagcgatgt agcggatccg acggtcggtg tggatttcta cgcccgttca atagacatcg
      901 aacctggggt taaaatcaaa ctacagttat gggatacagc cggacaggag agattcaggt
      961 ctatcaccac ttcctactat cgcaactctg tgggcggtct cttagttttt gacctgacga
     1021 acaggaagac ctttgatcat gtgcgggaat ggcaccgaga agtcagcgag cacatcctgc
     1081 ctcatcacat ggtctacatc ctgatcggcc acaagagcga cctgagccgc gatcgcaagg
     1141 tgacacgaga tgaggccgag cagctagcgg ccgatctcgg aatccgctat gtggaaacat
     1201 cagccaaatg caacagcaat gtggaacgtg ctttcgagct gctcacacgg gacatttatg
     1261 agctgatgaa gatgggtgag atctccactc gtgacggctg ggatggggtc aagagcggcc
     1321 tcactgccaa agttctttat ccagctgagg aggaagcgga gcgagaaaag agctgcaact
     1381 gctgacttcc acaatccaaa tcgaattccc gcggccgcca tggcggccgg gagcatgcga
     1441 cgtcgggccc aattcgccct atagtgagtc gtattacaat tcactggccg tcgttttaca
     1501 acgtcgtgac tgggaaaacc ctggcgttac ccaacttaat cgccttgcag cacatccccc
     1561 tttcgccagc tggcgtaata gcgaagaggc ccgcaccgat cgcccttccc aacagttgcg
     1621 cagcctgaat ggcgaatgga cgcgccctgt agcggcgcat taagcgcggc gggtgtggtg
     1681 gttacgcgca gcgtgaccgc tacacttgcc agcgccctag cgcccgctcc tttcgctttc
     1741 ttcccttcct ttctcgccac gttcgccggc tttccccgtc aagctctaaa tcgggggctc
     1801 cctttagggt tccgatttag tgctttacgg cacctcgacc ccaaaaaact tgattagggt
     1861 gatggttcac gtagtgggcc atcgccctga tagacggttt ttcgcccttt gacgttggag
     1921 tccacgttct ttaatagtgg actcttgttc caaactggaa caacactcaa ccctatctcg
     1981 gtctattctt ttgatttata agggattttg ccgatttcgg cctattggtt aaaaaatgag
     2041 ctgatttaac aaaaatttaa cgcgaatttt aacaaaatat taacgcttac aatttcctga
     2101 tgcggtattt tctccttacg catctgtgcg gtatttcaca ccgcatcagg tggcactttt
     2161 cggggaaatg tgcgcggaac ccctatttgt ttatttttct aaatacattc aaatatgtat
     2221 ccgctcatga gacaataacc ctgataaatg cttcaataat attgaaaaag gaagagtatg
     2281 agtattcaac atttccgtgt cgcccttatt cccttttttg cggcattttg ccttcctgtt
     2341 tttgctcacc cagaaacgct ggtgaaagta aaagatgctg aagatcagtt gggtgcacga
     2401 gtgggttaca tcgaactgga tctcaacagc ggtaagatcc ttgagagttt tcgccccgaa
     2461 gaacgttttc caatgatgag cacttttaaa gttctgctat gtggcgcggt attatcccgt
     2521 attgacgccg ggcaagagca actcggtcgc cgcatacact attctcagaa tgacttggtt
     2581 gagtactcac cagtcacaga aaagcatctt acggatggca tgacagtaag agaattatgc
     2641 agtgctgcca taaccatgag tgataacact gcggccaact tacttctgac aacgatcgga
     2701 ggaccgaagg agctaaccgc ttttttgcac aacatggggg atcatgtaac tcgccttgat
     2761 cgttgggaac cggagctgaa tgaagccata ccaaacgacg agcgtgacac cacgatgcct
     2821 gtagcaatgg caacaacgtt gcgcaaacta ttaactggcg aactacttac tctagcttcc
     2881 cggcaacaat taatagactg gatggaggcg gataaagttg caggaccact tctgcgctcg
     2941 gcccttccgg ctggctggtt tattgctgat aaatctggag ccggtgagcg tgggtctcgc
     3001 ggtatcattg cagcactggg gccagatggt aagccctccc gtatcgtagt tatctacacg
     3061 acggggagtc aggcaactat ggatgaacga aatagacaga tcgctgagat aggtgcctca
     3121 ctgattaagc attggtaact gtcagaccaa gtttactcat atatacttta gattgattta
     3181 aaacttcatt tttaatttaa aaggatctag gtgaagatcc tttttgataa tctcatgacc
     3241 aaaatccctt aacgtgagtt ttcgttccac tgagcgtcag accccgtaga aaagatcaaa
     3301 ggatcttctt gagatccttt ttttctgcgc gtaatctgct gcttgcaaac aaaaaaacca
     3361 ccgctaccag cggtggtttg tttgccggat caagagctac caactctttt tccgaaggta
     3421 actggcttca gcagagcgca gataccaaat actgttcttc tagtgtagcc gtagttaggc
     3481 caccacttca agaactctgt agcaccgcct acatacctcg ctctgctaat cctgttacca
     3541 gtggctgctg ccagtggcga taagtcgtgt cttaccgggt tggactcaag acgatagtta
     3601 ccggataagg cgcagcggtc gggctgaacg gggggttcgt gcacacagcc cagcttggag
     3661 cgaacgacct acacc
//
