LOCUS       sequence-176567-        3654 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Can be used for in situ hybridisation or other purposes.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3654)
  AUTHORS   Hall TE, Martel N, Lo HP, Xiong Z, Parton RG
  TITLE     A plasmid library of full-length zebrafish rab proteins for in vivo 
            cell biology.
  JOURNAL   Cell Logist. 2017 Mar 1;7(1):e1301151. doi: 
            10.1080/21592799.2017.1301151. eCollection 2017.
  PUBMED    28396820
REFERENCE   2  (bases 1 to 3654)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell 
            Logist."; date: "2017-03-1"; volume: "7(1):e1301151. doi"; pages: " 
            10.1080/21592799.2017.1301151. eCollection 2017"
COMMENT     Sequence Label: Rab30 cds in pGem-T Easy
FEATURES             Location/Qualifiers
     source          1..3654
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     101..120
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     354..371
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    488..509
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(524..529,530..547,548..554)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    562..578
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     567..589
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     586..602
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     586..602
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     promoter        620..638
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     620..637
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     regulatory      1371..1380
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     regulatory      1375..1384
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     primer_bind     complement(1414..1433)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(1415..1433)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(1440..1457)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(1440..1456)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(1449..1471)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      1597..2052
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(1684..1703)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     1894..1915
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        2130..2234
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(2235..2303,2304..3095)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(2453..2472)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      join(3266..3654,1..200)
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
ORIGIN
        1 agctatgaga aagcgccacg cttcccgaag ggagaaaggc ggacaggtat ccggtaagcg
       61 gcagggtcgg aacaggagag cgcacgaggg agcttccagg gggaaacgcc tggtatcttt
      121 atagtcctgt cgggtttcgc cacctctgac ttgagcgtcg atttttgtga tgctcgtcag
      181 gggggcggag cctatggaaa aacgccagca acgcggcctt tttacggttc ctggcctttt
      241 gctggccttt tgctcacatg ttctttcctg cgttatcccc tgattctgtg gataaccgta
      301 ttaccgcctt tgagtgagct gataccgctc gccgcagccg aacgaccgag cgcagcgagt
      361 cagtgagcga ggaagcggaa gagcgcccaa tacgcaaacc gcctctcccc gcgcgttggc
      421 cgattcatta atgcagctgg cacgacaggt ttcccgactg gaaagcgggc agtgagcgca
      481 acgcaattaa tgtgagttag ctcactcatt aggcacccca ggctttacac tttatgcttc
      541 cggctcgtat gttgtgtgga attgtgagcg gataacaatt tcacacagga aacagctatg
      601 accatgatta cgccaagcta tttaggtgac actatagaat actcaagcta tgcatccaac
      661 gcgttgggag ctctcccata tggtcgacct gcaggcggcc gcgaattcac tagtgattct
      721 gagtgtgagc atggaggatt atgattactt attcaaaatt gtgctgatag gaaatgcagg
      781 agtgggtaaa acctgcctgg tgcgacgttt cactcagggg ctgtttcctc ctggacaggg
      841 tgccacaatt ggagttgatt ttatgattaa aactgtggaa ataaaaggag ttaaggtgaa
      901 gctccagatt tgggacacag ctggtcaaga aagatttcgc tctataactc agagctacta
      961 ccgcagtgcc aatgccctga tcctcaccta cgacatcacc tgcgaagatt cgttccggtg
     1021 ccttcctgag tggctgaggg agattgagca gtacgccaac aaccaagtgg tgacaatcct
     1081 tgtagggaat aagatagact tggctgataa gcgagaagtc cttcgtcaac gagcagagga
     1141 gtttgcagat tctcagagca tgctttatct ggagacctct gccaaagaat cagacaacgt
     1201 ggagaagctc tttctagatc tggcctgcga actaatccgt gaggccaagc aaaacacgtt
     1261 ggacaacaac gactccactc ccatgcccgg agaagggaaa aacatcaact atttgggctg
     1321 ctgcagcatg aactagtaga cttggccacg ctgtcaatcg aattcccgcg gccgccatgg
     1381 cggccgggag catgcgacgt cgggcccaat tcgccctata gtgagtcgta ttacaattca
     1441 ctggccgtcg ttttacaacg tcgtgactgg gaaaaccctg gcgttaccca acttaatcgc
     1501 cttgcagcac atcccccttt cgccagctgg cgtaatagcg aagaggcccg caccgatcgc
     1561 ccttcccaac agttgcgcag cctgaatggc gaatggacgc gccctgtagc ggcgcattaa
     1621 gcgcggcggg tgtggtggtt acgcgcagcg tgaccgctac acttgccagc gccctagcgc
     1681 ccgctccttt cgctttcttc ccttcctttc tcgccacgtt cgccggcttt ccccgtcaag
     1741 ctctaaatcg ggggctccct ttagggttcc gatttagtgc tttacggcac ctcgacccca
     1801 aaaaacttga ttagggtgat ggttcacgta gtgggccatc gccctgatag acggtttttc
     1861 gccctttgac gttggagtcc acgttcttta atagtggact cttgttccaa actggaacaa
     1921 cactcaaccc tatctcggtc tattcttttg atttataagg gattttgccg atttcggcct
     1981 attggttaaa aaatgagctg atttaacaaa aatttaacgc gaattttaac aaaatattaa
     2041 cgcttacaat ttcctgatgc ggtattttct ccttacgcat ctgtgcggta tttcacaccg
     2101 catcaggtgg cacttttcgg ggaaatgtgc gcggaacccc tatttgttta tttttctaaa
     2161 tacattcaaa tatgtatccg ctcatgagac aataaccctg ataaatgctt caataatatt
     2221 gaaaaaggaa gagtatgagt attcaacatt tccgtgtcgc ccttattccc ttttttgcgg
     2281 cattttgcct tcctgttttt gctcacccag aaacgctggt gaaagtaaaa gatgctgaag
     2341 atcagttggg tgcacgagtg ggttacatcg aactggatct caacagcggt aagatccttg
     2401 agagttttcg ccccgaagaa cgttttccaa tgatgagcac ttttaaagtt ctgctatgtg
     2461 gcgcggtatt atcccgtatt gacgccgggc aagagcaact cggtcgccgc atacactatt
     2521 ctcagaatga cttggttgag tactcaccag tcacagaaaa gcatcttacg gatggcatga
     2581 cagtaagaga attatgcagt gctgccataa ccatgagtga taacactgcg gccaacttac
     2641 ttctgacaac gatcggagga ccgaaggagc taaccgcttt tttgcacaac atgggggatc
     2701 atgtaactcg ccttgatcgt tgggaaccgg agctgaatga agccatacca aacgacgagc
     2761 gtgacaccac gatgcctgta gcaatggcaa caacgttgcg caaactatta actggcgaac
     2821 tacttactct agcttcccgg caacaattaa tagactggat ggaggcggat aaagttgcag
     2881 gaccacttct gcgctcggcc cttccggctg gctggtttat tgctgataaa tctggagccg
     2941 gtgagcgtgg gtctcgcggt atcattgcag cactggggcc agatggtaag ccctcccgta
     3001 tcgtagttat ctacacgacg gggagtcagg caactatgga tgaacgaaat agacagatcg
     3061 ctgagatagg tgcctcactg attaagcatt ggtaactgtc agaccaagtt tactcatata
     3121 tactttagat tgatttaaaa cttcattttt aatttaaaag gatctaggtg aagatccttt
     3181 ttgataatct catgaccaaa atcccttaac gtgagttttc gttccactga gcgtcagacc
     3241 ccgtagaaaa gatcaaagga tcttcttgag atcctttttt tctgcgcgta atctgctgct
     3301 tgcaaacaaa aaaaccaccg ctaccagcgg tggtttgttt gccggatcaa gagctaccaa
     3361 ctctttttcc gaaggtaact ggcttcagca gagcgcagat accaaatact gttcttctag
     3421 tgtagccgta gttaggccac cacttcaaga actctgtagc accgcctaca tacctcgctc
     3481 tgctaatcct gttaccagtg gctgctgcca gtggcgataa gtcgtgtctt accgggttgg
     3541 actcaagacg atagttaccg gataaggcgc agcggtcggg ctgaacgggg ggttcgtgca
     3601 cacagcccag cttggagcga acgacctaca ccgaactgag atacctacag cgtg
//
