LOCUS       sequence-176571-        4179 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Express Rotavirus SA11 NSP3 in mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4179)
  AUTHORS   Kanai Y, Komoto S, Kawagishi T, Nouda R, Nagasawa N, Onishi M, 
            Matsuura Y, Taniguchi K, Kobayashi T
  TITLE     Entirely plasmid-based reverse genetics system for rotaviruses.
  JOURNAL   Proc Natl Acad Sci U S A. 2017 Jan 30. pii: 201618424. doi: 
            10.1073/pnas.1618424114.
  PUBMED    28137864
REFERENCE   2  (bases 1 to 4179)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Proc Natl 
            Acad Sci U S A. 2017 Jan 30. pii: 201618424. doi: 
            10.1073/pnas.1618424114."
COMMENT     Sequence Label: pT7-NSP3SA11
FEATURES             Location/Qualifiers
     source          1..4179
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(30..47)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(201..789)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(281..300)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(960..1751,1752..1820))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     1583..1602
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(1821..1925)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     1993..2011
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     complement(2049..2071)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     2171..2190
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     2400..2419
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
     promoter        2709..2727
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     2709..2726
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     terminator      complement(2795..2842)
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     primer_bind     2838..2856
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
     promoter        complement(4106..4124)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
ORIGIN
        1 ggtttgcgta ttgggcgctc ttccgcttcc tcgctcactg actcgctgcg ctcggtcgtt
       61 cggctgcggc gagcggtatc agctcactca aaggcggtaa tacggttatc cacagaatca
      121 ggggataacg caggaaagaa catgtgagca aaaggccagc aaaaggccag gaaccgtaaa
      181 aaggccgcgt tgctggcgtt tttccatagg ctccgccccc ctgacgagca tcacaaaaat
      241 cgacgctcaa gtcagaggtg gcgaaacccg acaggactat aaagatacca ggcgtttccc
      301 cctggaagct ccctcgtgcg ctctcctgtt ccgaccctgc cgcttaccgg atacctgtcc
      361 gcctttctcc cttcgggaag cgtggcgctt tctcatagct cacgctgtag gtatctcagt
      421 tcggtgtagg tcgttcgctc caagctgggc tgtgtgcacg aaccccccgt tcagcccgac
      481 cgctgcgcct tatccggtaa ctatcgtctt gagtccaacc cggtaagaca cgacttatcg
      541 ccactggcag cagccactgg taacaggatt agcagagcga ggtatgtagg cggtgctaca
      601 gagttcttga agtggtggcc taactacggc tacactagaa gaacagtatt tggtatctgc
      661 gctctgctga agccagttac cttcggaaaa agagttggta gctcttgatc cggcaaacaa
      721 accaccgctg gtagcggtgg tttttttgtt tgcaagcagc agattacgcg cagaaaaaaa
      781 ggatctcaag aagatccttt gatcttttct acggggtctg acgctcagtg gaacgaaaac
      841 tcacgttaag ggattttggt catgagatta tcaaaaagga tcttcaccta gatcctttta
      901 aattaaaaat gaagttttaa atcaatctaa agtatatatg agtaaacttg gtctgacagt
      961 taccaatgct taatcagtga ggcacctatc tcagcgatct gtctatttcg ttcatccata
     1021 gttgcctgac tccccgtcgt gtagataact acgatacggg agggcttacc atctggcccc
     1081 agtgctgcaa tgataccgcg agacccacgc tcaccggctc cagatttatc agcaataaac
     1141 cagccagccg gaagggccga gcgcagaagt ggtcctgcaa ctttatccgc ctccatccag
     1201 tctattaatt gttgccggga agctagagta agtagttcgc cagttaatag tttgcgcaac
     1261 gttgttgcca ttgctacagg catcgtggtg tcacgctcgt cgtttggtat ggcttcattc
     1321 agctccggtt cccaacgatc aaggcgagtt acatgatccc ccatgttgtg caaaaaagcg
     1381 gttagctcct tcggtcctcc gatcgttgtc agaagtaagt tggccgcagt gttatcactc
     1441 atggttatgg cagcactgca taattctctt actgtcatgc catccgtaag atgcttttct
     1501 gtgactggtg agtactcaac caagtcattc tgagaatagt gtatgcggcg accgagttgc
     1561 tcttgcccgg cgtcaatacg ggataatacc gcgccacata gcagaacttt aaaagtgctc
     1621 atcattggaa aacgttcttc ggggcgaaaa ctctcaagga tcttaccgct gttgagatcc
     1681 agttcgatgt aacccactcg tgcacccaac tgatcttcag catcttttac tttcaccagc
     1741 gtttctgggt gagcaaaaac aggaaggcaa aatgccgcaa aaaagggaat aagggcgaca
     1801 cggaaatgtt gaatactcat actcttcctt tttcaatatt attgaagcat ttatcagggt
     1861 tattgtctca tgagcggata catatttgaa tgtatttaga aaaataaaca aataggggtt
     1921 ccgcgcacat ttccccgaaa agtgccacct gacgtctaag aaaccattat tatcatgaca
     1981 ttaacctata aaaataggcg tatcacgagg ccctttcgtc tcgcgcgttt cggtgatgac
     2041 ggtgaaaacc tctgacacat gcagctcccg gagacggtca cagcttgtct gtaagcggat
     2101 gccgggagca gacaagcccg tcagggcgcg tcagcgggtg ttggcgggtg tcggggctgg
     2161 cttaactatg cggcatcaga gcagattgta ctgagagtgc accattcgac gctctccctt
     2221 atgcgactcc tgcattaaga agcagcccag tagtaggttg aggccgttga gcaccgccgc
     2281 cgcaaggaat ggtgcaagga gatggcgccc aacagtcccc cggccacggg gcctgccacc
     2341 atacccacgc cgaaacaagc gctcatgagc ccgaagtggc gagcccgatc ttccccatcg
     2401 gtgatgtcgg cgatataggc gccagcaacc gcacctgtgg cgccggtgat gccggccacg
     2461 atgcgtccgg cgtagaggat ctggctagcg atgaccctgc tgattggttc gctgaccatt
     2521 tccgggtgcg ggacggcgtt accagaaact cagaaggttc gtccaaccaa accgactctg
     2581 acggcagttt acgagagaga tgatagggtc tgcttcagta agccagatgc tacacaatta
     2641 ggcttgtaca tattgtcgtt agaacgcggc tacaattaat acataacctt atgtatcata
     2701 cacatacgat ttaggtgaca ctatagaata caagcttgca tgcctgcagg tcgactctag
     2761 aggatctcga tccggatata gttcctcctt tcagcaaaaa acccctcaag acccgtttag
     2821 aggccccaag gggttatgct agttattgct cagcggtggc agcagccaac tcagcttcct
     2881 ttcgggcttt gttagcagcc ggatcctttt ttttgagctc tcccttagcc atccgagtgg
     2941 acgacgtcct ccttcggatg cccaggtcgg accgcgagga ggtggagatg ccatgccgac
     3001 ccggccacat aacgccccta tagccattta ggtttttgac agtgttagct tttaactatt
     3061 gtgctcatag agggttatgc atagatgaca acatactaac actgtatttg attgttcaac
     3121 atgtatcaaa atggttattc gcaaccaaat gaatattgat aattacatct ctgtattaat
     3181 cctttgaaca taaggaataa tttgtcataa tcagatatca aattacgtat tactgattct
     3241 aagtcatcaa aagcgtgcaa tggatttatc gcatcaattg agttaatttg ctgttcaatg
     3301 tctgccttta tttcagggtc taattccatt gatcttaaat accattcaat cgaagaagta
     3361 agggatccaa ttttattttg caaatcacgt tctagtttat tattgtacac ttgaagctca
     3421 gctatatgtg cttgctgttg agagatgaca ttttgaagag aatgcatatt ttcattcatt
     3481 tttcttgctt tcaacaccca attattatat ttttcattta ccctggattt caatgattca
     3541 aacctttgct ccaattgctc atagcgcgat ttccagtcaa tggtatccac ttccattttt
     3601 tcatccacaa atgaatcatc cacttcaact tcaccacgtt ccaatttatc acgcatcaat
     3661 tttgtgcatt taataataga tgatgatttt ccaggtattc tttttacact gaagcatgcg
     3721 tttaaaactc tcattttttg atcaattcct tttgatgata acatcattct tagtttgttt
     3781 acatcctcat ctaatttagc tgctctagaa gtatcagcaa gccagtttct atttcttata
     3841 gcagatccaa atttattatt caaagcttga tcaatagtta ttgctttacc aatcagatta
     3901 tttttaacac cagaatcgtc catcacaaaa tcaaatttat tctttactct agaatatata
     3961 tcctgataat catattctat tcccatattc tcaagagctg aggttgcagc tacaactgca
     4021 gcttcaaaag aagagttaat aattgagacg gccatctgtt gcgtagactc catcttgagc
     4081 atcaaccact gaaaagcatt aaatgcctat agtgagtcgt attaacccgg gatcgatatc
     4141 cccctgcatt aatgaatcgg ccaacgcgcg gggagaggc
//
