LOCUS       sequence-176572-        4136 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Express Rotavirus SA11 VP7 in mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4136)
  AUTHORS   Kanai Y, Komoto S, Kawagishi T, Nouda R, Nagasawa N, Onishi M, 
            Matsuura Y, Taniguchi K, Kobayashi T
  TITLE     Entirely plasmid-based reverse genetics system for rotaviruses.
  JOURNAL   Proc Natl Acad Sci U S A. 2017 Jan 30. pii: 201618424. doi: 
            10.1073/pnas.1618424114.
  PUBMED    28137864
REFERENCE   2  (bases 1 to 4136)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Proc Natl 
            Acad Sci U S A. 2017 Jan 30. pii: 201618424. doi: 
            10.1073/pnas.1618424114."
COMMENT     Sequence Label: pT7-VP7SA11
FEATURES             Location/Qualifiers
     source          1..4136
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(40..62)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     162..181
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     391..410
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
     promoter        700..718
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     700..717
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     terminator      complement(786..833)
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     primer_bind     829..847
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
     promoter        complement(2055..2073)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(2157..2174)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(2328..2916)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(2408..2427)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(3087..3878,3879..3947))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     3710..3729
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(3948..4052)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     join(4120..4136,1..2)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
ORIGIN
        1 gccctttcgt ctcgcgcgtt tcggtgatga cggtgaaaac ctctgacaca tgcagctccc
       61 ggagacggtc acagcttgtc tgtaagcgga tgccgggagc agacaagccc gtcagggcgc
      121 gtcagcgggt gttggcgggt gtcggggctg gcttaactat gcggcatcag agcagattgt
      181 actgagagtg caccattcga cgctctccct tatgcgactc ctgcattaag aagcagccca
      241 gtagtaggtt gaggccgttg agcaccgccg ccgcaaggaa tggtgcaagg agatggcgcc
      301 caacagtccc ccggccacgg ggcctgccac catacccacg ccgaaacaag cgctcatgag
      361 cccgaagtgg cgagcccgat cttccccatc ggtgatgtcg gcgatatagg cgccagcaac
      421 cgcacctgtg gcgccggtga tgccggccac gatgcgtccg gcgtagagga tctggctagc
      481 gatgaccctg ctgattggtt cgctgaccat ttccgggtgc gggacggcgt taccagaaac
      541 tcagaaggtt cgtccaacca aaccgactct gacggcagtt tacgagagag atgatagggt
      601 ctgcttcagt aagccagatg ctacacaatt aggcttgtac atattgtcgt tagaacgcgg
      661 ctacaattaa tacataacct tatgtatcat acacatacga tttaggtgac actatagaat
      721 acaagcttgc atgcctgcag gtcgactcta gaggatctcg atccggatat agttcctcct
      781 ttcagcaaaa aacccctcaa gacccgttta gaggccccaa ggggttatgc tagttattgc
      841 tcagcggtgg cagcagccaa ctcagcttcc tttcgggctt tgttagcagc cggatccttt
      901 tttttgagct ctcccttagc catccgagtg gacgacgtcc tccttcggat gcccaggtcg
      961 gaccgcgagg aggtggagat gccatgccga cccggtcaca tcatacaatt ctaacctaag
     1021 ttatacctac actctgtaat aaaatgctgc tgaatttagt gatcttgatc ttttggacat
     1081 aacttgtatt atctgatcta catagtctac tacagtataa aaaacttgcc accatttttt
     1141 ccagttaatt cgcatcatcc gttctgtctg tggtgcagta gttggatcag cagttatatc
     1201 gaggatgtca gaaccaccaa cttgtataac ggctacgttt tctcttggtc ccaatttctt
     1261 acagtttcta atagtacacg ttgctgttgt gacatccagc ttatgattaa cgccatcaac
     1321 cacgtcagta attaccaact tttcagctgt cgcaacttct tcaaaagttg tagcatcagt
     1381 tgtcaagcat ccaattccaa gagtttgtgt attaagtgga catactttaa ttgtacatga
     1441 tgagcccatt gatatccatt tattcgcttc gtcagtttgc tgataataat acagagtaat
     1501 atccattgga ttacacaacc attcgtttaa tattagatcc gcaagttctg acatatccaa
     1561 ttgcaacgtc gcgtcatatt tcattagtac tacgttataa tcacaataca actgcggatc
     1621 aacagaaaac gatgcaatgt tagtatattc tttaaaatat acggatccag ttggccaccc
     1681 tttcgtaaga aatagttgtg acagtgtgtc tttccatgaa ttatcgttta tttcagtcgc
     1741 agcctcagtc ggataatata ggcaaagtgt agaagtgagg aatggttctt cttgcgttga
     1801 attagcgtat gcaatgtcca tggagcctgt gattggaaga ttaataccat aattttgtgc
     1861 tctgagaaat ggtgacaata tcacaattat aaaaagaaat ctataaatta taaagtccat
     1921 tattctagtt aatgatttaa gtatgtaatt tagtagaata atcgatatca gaaaggttag
     1981 aactgtggta tattcaatac catacattaa aaggagctaa ccgctagcca aacggaaatt
     2041 ctctcttttt aaagcctata gtgagtcgta ttaaccggga tcgatatccc cctgcattaa
     2101 tgaatcggcc aacgcgcggg gagaggcggt ttgcgtattg ggcgctcttc cgcttcctcg
     2161 ctcactgact cgctgcgctc ggtcgttcgg ctgcggcgag cggtatcagc tcactcaaag
     2221 gcggtaatac ggttatccac agaatcaggg gataacgcag gaaagaacat gtgagcaaaa
     2281 ggccagcaaa aggccaggaa ccgtaaaaag gccgcgttgc tggcgttttt ccataggctc
     2341 cgcccccctg acgagcatca caaaaatcga cgctcaagtc agaggtggcg aaacccgaca
     2401 ggactataaa gataccaggc gtttccccct ggaagctccc tcgtgcgctc tcctgttccg
     2461 accctgccgc ttaccggata cctgtccgcc tttctccctt cgggaagcgt ggcgctttct
     2521 catagctcac gctgtaggta tctcagttcg gtgtaggtcg ttcgctccaa gctgggctgt
     2581 gtgcacgaac cccccgttca gcccgaccgc tgcgccttat ccggtaacta tcgtcttgag
     2641 tccaacccgg taagacacga cttatcgcca ctggcagcag ccactggtaa caggattagc
     2701 agagcgaggt atgtaggcgg tgctacagag ttcttgaagt ggtggcctaa ctacggctac
     2761 actagaagaa cagtatttgg tatctgcgct ctgctgaagc cagttacctt cggaaaaaga
     2821 gttggtagct cttgatccgg caaacaaacc accgctggta gcggtggttt ttttgtttgc
     2881 aagcagcaga ttacgcgcag aaaaaaagga tctcaagaag atcctttgat cttttctacg
     2941 gggtctgacg ctcagtggaa cgaaaactca cgttaaggga ttttggtcat gagattatca
     3001 aaaaggatct tcacctagat ccttttaaat taaaaatgaa gttttaaatc aatctaaagt
     3061 atatatgagt aaacttggtc tgacagttac caatgcttaa tcagtgaggc acctatctca
     3121 gcgatctgtc tatttcgttc atccatagtt gcctgactcc ccgtcgtgta gataactacg
     3181 atacgggagg gcttaccatc tggccccagt gctgcaatga taccgcgaga cccacgctca
     3241 ccggctccag atttatcagc aataaaccag ccagccggaa gggccgagcg cagaagtggt
     3301 cctgcaactt tatccgcctc catccagtct attaattgtt gccgggaagc tagagtaagt
     3361 agttcgccag ttaatagttt gcgcaacgtt gttgccattg ctacaggcat cgtggtgtca
     3421 cgctcgtcgt ttggtatggc ttcattcagc tccggttccc aacgatcaag gcgagttaca
     3481 tgatccccca tgttgtgcaa aaaagcggtt agctccttcg gtcctccgat cgttgtcaga
     3541 agtaagttgg ccgcagtgtt atcactcatg gttatggcag cactgcataa ttctcttact
     3601 gtcatgccat ccgtaagatg cttttctgtg actggtgagt actcaaccaa gtcattctga
     3661 gaatagtgta tgcggcgacc gagttgctct tgcccggcgt caatacggga taataccgcg
     3721 ccacatagca gaactttaaa agtgctcatc attggaaaac gttcttcggg gcgaaaactc
     3781 tcaaggatct taccgctgtt gagatccagt tcgatgtaac ccactcgtgc acccaactga
     3841 tcttcagcat cttttacttt caccagcgtt tctgggtgag caaaaacagg aaggcaaaat
     3901 gccgcaaaaa agggaataag ggcgacacgg aaatgttgaa tactcatact cttccttttt
     3961 caatattatt gaagcattta tcagggttat tgtctcatga gcggatacat atttgaatgt
     4021 atttagaaaa ataaacaaat aggggttccg cgcacatttc cccgaaaagt gccacctgac
     4081 gtctaagaaa ccattattat catgacatta acctataaaa ataggcgtat cacgag
//
