LOCUS       sequence-176575-        4430 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Express Rotavirus SA11 VP6 in mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4430)
  AUTHORS   Kanai Y, Komoto S, Kawagishi T, Nouda R, Nagasawa N, Onishi M, 
            Matsuura Y, Taniguchi K, Kobayashi T
  TITLE     Entirely plasmid-based reverse genetics system for rotaviruses.
  JOURNAL   Proc Natl Acad Sci U S A. 2017 Jan 30. pii: 201618424. doi: 
            10.1073/pnas.1618424114.
  PUBMED    28137864
REFERENCE   2  (bases 1 to 4430)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Proc Natl 
            Acad Sci U S A. 2017 Jan 30. pii: 201618424. doi: 
            10.1073/pnas.1618424114."
COMMENT     Sequence Label: pT7-VP6SA11
FEATURES             Location/Qualifiers
     source          1..4430
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(29..46)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(200..788)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(280..299)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(959..1750,1751..1819))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     1582..1601
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(1820..1924)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     1992..2010
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     complement(2048..2070)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     2170..2189
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     2399..2418
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
     promoter        2708..2726
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     2708..2725
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     terminator      complement(2794..2841)
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     primer_bind     2837..2855
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
     promoter        complement(4356..4374)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
ORIGIN
        1 gtttgcgtat tgggcgctct tccgcttcct cgctcactga ctcgctgcgc tcggtcgttc
       61 ggctgcggcg agcggtatca gctcactcaa aggcggtaat acggttatcc acagaatcag
      121 gggataacgc aggaaagaac atgtgagcaa aaggccagca aaaggccagg aaccgtaaaa
      181 aggccgcgtt gctggcgttt ttccataggc tccgcccccc tgacgagcat cacaaaaatc
      241 gacgctcaag tcagaggtgg cgaaacccga caggactata aagataccag gcgtttcccc
      301 ctggaagctc cctcgtgcgc tctcctgttc cgaccctgcc gcttaccgga tacctgtccg
      361 cctttctccc ttcgggaagc gtggcgcttt ctcatagctc acgctgtagg tatctcagtt
      421 cggtgtaggt cgttcgctcc aagctgggct gtgtgcacga accccccgtt cagcccgacc
      481 gctgcgcctt atccggtaac tatcgtcttg agtccaaccc ggtaagacac gacttatcgc
      541 cactggcagc agccactggt aacaggatta gcagagcgag gtatgtaggc ggtgctacag
      601 agttcttgaa gtggtggcct aactacggct acactagaag aacagtattt ggtatctgcg
      661 ctctgctgaa gccagttacc ttcggaaaaa gagttggtag ctcttgatcc ggcaaacaaa
      721 ccaccgctgg tagcggtggt ttttttgttt gcaagcagca gattacgcgc agaaaaaaag
      781 gatctcaaga agatcctttg atcttttcta cggggtctga cgctcagtgg aacgaaaact
      841 cacgttaagg gattttggtc atgagattat caaaaaggat cttcacctag atccttttaa
      901 attaaaaatg aagttttaaa tcaatctaaa gtatatatga gtaaacttgg tctgacagtt
      961 accaatgctt aatcagtgag gcacctatct cagcgatctg tctatttcgt tcatccatag
     1021 ttgcctgact ccccgtcgtg tagataacta cgatacggga gggcttacca tctggcccca
     1081 gtgctgcaat gataccgcga gacccacgct caccggctcc agatttatca gcaataaacc
     1141 agccagccgg aagggccgag cgcagaagtg gtcctgcaac tttatccgcc tccatccagt
     1201 ctattaattg ttgccgggaa gctagagtaa gtagttcgcc agttaatagt ttgcgcaacg
     1261 ttgttgccat tgctacaggc atcgtggtgt cacgctcgtc gtttggtatg gcttcattca
     1321 gctccggttc ccaacgatca aggcgagtta catgatcccc catgttgtgc aaaaaagcgg
     1381 ttagctcctt cggtcctccg atcgttgtca gaagtaagtt ggccgcagtg ttatcactca
     1441 tggttatggc agcactgcat aattctctta ctgtcatgcc atccgtaaga tgcttttctg
     1501 tgactggtga gtactcaacc aagtcattct gagaatagtg tatgcggcga ccgagttgct
     1561 cttgcccggc gtcaatacgg gataataccg cgccacatag cagaacttta aaagtgctca
     1621 tcattggaaa acgttcttcg gggcgaaaac tctcaaggat cttaccgctg ttgagatcca
     1681 gttcgatgta acccactcgt gcacccaact gatcttcagc atcttttact ttcaccagcg
     1741 tttctgggtg agcaaaaaca ggaaggcaaa atgccgcaaa aaagggaata agggcgacac
     1801 ggaaatgttg aatactcata ctcttccttt ttcaatatta ttgaagcatt tatcagggtt
     1861 attgtctcat gagcggatac atatttgaat gtatttagaa aaataaacaa ataggggttc
     1921 cgcgcacatt tccccgaaaa gtgccacctg acgtctaaga aaccattatt atcatgacat
     1981 taacctataa aaataggcgt atcacgaggc cctttcgtct cgcgcgtttc ggtgatgacg
     2041 gtgaaaacct ctgacacatg cagctcccgg agacggtcac agcttgtctg taagcggatg
     2101 ccgggagcag acaagcccgt cagggcgcgt cagcgggtgt tggcgggtgt cggggctggc
     2161 ttaactatgc ggcatcagag cagattgtac tgagagtgca ccattcgacg ctctccctta
     2221 tgcgactcct gcattaagaa gcagcccagt agtaggttga ggccgttgag caccgccgcc
     2281 gcaaggaatg gtgcaaggag atggcgccca acagtccccc ggccacgggg cctgccacca
     2341 tacccacgcc gaaacaagcg ctcatgagcc cgaagtggcg agcccgatct tccccatcgg
     2401 tgatgtcggc gatataggcg ccagcaaccg cacctgtggc gccggtgatg ccggccacga
     2461 tgcgtccggc gtagaggatc tggctagcga tgaccctgct gattggttcg ctgaccattt
     2521 ccgggtgcgg gacggcgtta ccagaaactc agaaggttcg tccaaccaaa ccgactctga
     2581 cggcagttta cgagagagat gatagggtct gcttcagtaa gccagatgct acacaattag
     2641 gcttgtacat attgtcgtta gaacgcggct acaattaata cataacctta tgtatcatac
     2701 acatacgatt taggtgacac tatagaatac aagcttgcat gcctgcaggt cgactctaga
     2761 ggatctcgat ccggatatag ttcctccttt cagcaaaaaa cccctcaaga cccgtttaga
     2821 ggccccaagg ggttatgcta gttattgctc agcggtggca gcagccaact cagcttcctt
     2881 tcgggctttg ttagcagccg gatccttttt tttgagctct cccttagcca tccgagtgga
     2941 cgacgtcctc cttcggatgc ccaggtcgga ccgcgaggag gtggagatgc catgccgacc
     3001 cggtcacatc ctctcactat accatctgag tgattactct gtgtagcgaa tgcgtatacg
     3061 catccttact tacagagttc aaacagcttg atatagctac atactcacca aagttggata
     3121 ccaagttgtt agcttggtcc tcatttaatg agcatgcttc taatggaagc cactgtaaat
     3181 acgcgttgca aattgtcctc cctagacggt gaataattgg tgattaaatc agtccagttc
     3241 atacctggtg gaaagactgg tccaactggt attgcgtact cttgcctaac ggatgttaca
     3301 tttgctaata gagtttcact tgcatcggcg agtacagact cacaaactgc agactcaatt
     3361 ctaagtgtca atcccactgt tgcatgatgt tcgaatggct gtgcattcgg gaatagtact
     3421 gctactgctg gtgtcatgtt tggtggtctc attaactgga atgatagtct aatagtatca
     3481 aaatttctag ctacgatagt tccaaatctt gcttgataag tgtttattat ctgtccattc
     3541 aatagaaact ccacttcaac gttattcggc ctgagaatca ctgggttgaa aaaccatgta
     3601 gttgccccgt cagctgaatt gatcactctt ggaaaactaa acctttccgc gtctggtaga
     3661 agagttatcg tagctgtagt taacactctt cggagtggca caatatgctc aaattgttgt
     3721 atattggctg gtgcgttaat agcacatgag tagtcaaatc cagcgacttg aatttccgat
     3781 cctgcgttta accacattgt gcccatcaaa ttatcatgag cgggttgtga tctatttagt
     3841 gtaaatgatg ctgaataagg aaaaatgttt ggtttatgaa aagtgaaacc tgtcctctgt
     3901 cttctatttt gcaaattcca gttttctatg tattccgacg aattatcaaa atttattctt
     3961 ttgaatttaa tggctgacag ctttcttagc gagtctgatt gaggtgcaat tccgttcctt
     4021 tgtgattctc taaccatctc atccatgcat acattgtcta caaaatccac gaaataatca
     4081 attgtatttc ttgccgtttc aacataatta gcgtctaagt tcagcaaagt tgttccaagt
     4141 aacccgaaat taaaattcca gtttctaatt ggcaaattac cgattcctcc agtttgaaat
     4201 tcatttccat tcatagtaat tatcatttga ttaaattgtt gaattagatc actcacgtta
     4261 gaatacaatg tgccttcgac aattttgtct ctagcgtctt taagagtctt tgacaaagag
     4321 tataggacat ccatgttgaa gacttcgttt aaaagcctat agtgagtcgt attaacccgg
     4381 gatcgatatc cccctgcatt aatgaatcgg ccaacgcgcg gggagaggcg
//
