LOCUS       sequence-176582-        3602 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Can be used for in situ hybridisation or other purposes.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3602)
  AUTHORS   Hall TE, Martel N, Lo HP, Xiong Z, Parton RG
  TITLE     A plasmid library of full-length zebrafish rab proteins for in vivo 
            cell biology.
  JOURNAL   Cell Logist. 2017 Mar 1;7(1):e1301151. doi: 
            10.1080/21592799.2017.1301151. eCollection 2017.
  PUBMED    28396820
REFERENCE   2  (bases 1 to 3602)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell 
            Logist."; date: "2017-03-1"; volume: "7(1):e1301151. doi"; pages: " 
            10.1080/21592799.2017.1301151. eCollection 2017"
COMMENT     Sequence Label: Rab31 cds in pGem-T Easy
FEATURES             Location/Qualifiers
     source          1..3602
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     184..205
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        420..524
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(525..593,594..1385)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(743..762)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      1556..2144
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     2045..2064
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     2298..2315
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    2432..2453
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(2468..2473,2474..2491,2492..2498)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    2506..2522
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     2511..2533
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     2530..2546
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     2530..2546
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     promoter        2564..2582
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     2564..2581
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     regulatory      3263..3272
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     regulatory      3267..3276
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     primer_bind     complement(3306..3325)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(3307..3325)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(3332..3349)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(3332..3348)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(3341..3363)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      join(3489..3602,1..342)
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(3576..3595)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
ORIGIN
        1 tcgccacgtt cgccggcttt ccccgtcaag ctctaaatcg ggggctccct ttagggttcc
       61 gatttagtgc tttacggcac ctcgacccca aaaaacttga ttagggtgat ggttcacgta
      121 gtgggccatc gccctgatag acggtttttc gccctttgac gttggagtcc acgttcttta
      181 atagtggact cttgttccaa actggaacaa cactcaaccc tatctcggtc tattcttttg
      241 atttataagg gattttgccg atttcggcct attggttaaa aaatgagctg atttaacaaa
      301 aatttaacgc gaattttaac aaaatattaa cgcttacaat ttcctgatgc ggtattttct
      361 ccttacgcat ctgtgcggta tttcacaccg catcaggtgg cacttttcgg ggaaatgtgc
      421 gcggaacccc tatttgttta tttttctaaa tacattcaaa tatgtatccg ctcatgagac
      481 aataaccctg ataaatgctt caataatatt gaaaaaggaa gagtatgagt attcaacatt
      541 tccgtgtcgc ccttattccc ttttttgcgg cattttgcct tcctgttttt gctcacccag
      601 aaacgctggt gaaagtaaaa gatgctgaag atcagttggg tgcacgagtg ggttacatcg
      661 aactggatct caacagcggt aagatccttg agagttttcg ccccgaagaa cgttttccaa
      721 tgatgagcac ttttaaagtt ctgctatgtg gcgcggtatt atcccgtatt gacgccgggc
      781 aagagcaact cggtcgccgc atacactatt ctcagaatga cttggttgag tactcaccag
      841 tcacagaaaa gcatcttacg gatggcatga cagtaagaga attatgcagt gctgccataa
      901 ccatgagtga taacactgcg gccaacttac ttctgacaac gatcggagga ccgaaggagc
      961 taaccgcttt tttgcacaac atgggggatc atgtaactcg ccttgatcgt tgggaaccgg
     1021 agctgaatga agccatacca aacgacgagc gtgacaccac gatgcctgta gcaatggcaa
     1081 caacgttgcg caaactatta actggcgaac tacttactct agcttcccgg caacaattaa
     1141 tagactggat ggaggcggat aaagttgcag gaccacttct gcgctcggcc cttccggctg
     1201 gctggtttat tgctgataaa tctggagccg gtgagcgtgg gtctcgcggt atcattgcag
     1261 cactggggcc agatggtaag ccctcccgta tcgtagttat ctacacgacg gggagtcagg
     1321 caactatgga tgaacgaaat agacagatcg ctgagatagg tgcctcactg attaagcatt
     1381 ggtaactgtc agaccaagtt tactcatata tactttagat tgatttaaaa cttcattttt
     1441 aatttaaaag gatctaggtg aagatccttt ttgataatct catgaccaaa atcccttaac
     1501 gtgagttttc gttccactga gcgtcagacc ccgtagaaaa gatcaaagga tcttcttgag
     1561 atcctttttt tctgcgcgta atctgctgct tgcaaacaaa aaaaccaccg ctaccagcgg
     1621 tggtttgttt gccggatcaa gagctaccaa ctctttttcc gaaggtaact ggcttcagca
     1681 gagcgcagat accaaatact gttcttctag tgtagccgta gttaggccac cacttcaaga
     1741 actctgtagc accgcctaca tacctcgctc tgctaatcct gttaccagtg gctgctgcca
     1801 gtggcgataa gtcgtgtctt accgggttgg actcaagacg atagttaccg gataaggcgc
     1861 agcggtcggg ctgaacgggg ggttcgtgca cacagcccag cttggagcga acgacctaca
     1921 ccgaactgag atacctacag cgtgagctat gagaaagcgc cacgcttccc gaagggagaa
     1981 aggcggacag gtatccggta agcggcaggg tcggaacagg agagcgcacg agggagcttc
     2041 cagggggaaa cgcctggtat ctttatagtc ctgtcgggtt tcgccacctc tgacttgagc
     2101 gtcgattttt gtgatgctcg tcaggggggc ggagcctatg gaaaaacgcc agcaacgcgg
     2161 cctttttacg gttcctggcc ttttgctggc cttttgctca catgttcttt cctgcgttat
     2221 cccctgattc tgtggataac cgtattaccg cctttgagtg agctgatacc gctcgccgca
     2281 gccgaacgac cgagcgcagc gagtcagtga gcgaggaagc ggaagagcgc ccaatacgca
     2341 aaccgcctct ccccgcgcgt tggccgattc attaatgcag ctggcacgac aggtttcccg
     2401 actggaaagc gggcagtgag cgcaacgcaa ttaatgtgag ttagctcact cattaggcac
     2461 cccaggcttt acactttatg cttccggctc gtatgttgtg tggaattgtg agcggataac
     2521 aatttcacac aggaaacagc tatgaccatg attacgccaa gctatttagg tgacactata
     2581 gaatactcaa gctatgcatc caacgcgttg ggagctctcc catatggtcg acctgcaggc
     2641 ggccgcgaat tcactagtga ttatggccat aagggagcta aaagtttgtc tgctcgggga
     2701 cactggtgtg gggaaatcga gtatcgtgtg tcggtttgtt caagatcact ttgaccacaa
     2761 cattagtccg acaataggtg catcattttt aactaagacc gtcccctctg gaaatgaact
     2821 tcacaaattt ctgatctggg acactgcagg acaagagcgg tttcattccc tggctcccat
     2881 gtattacaga ggctcggcag cggctgtcat tgtttatgac atcacaaagc ttgactcgtt
     2941 ccagacgtta aagaagtggg tgaaagagct gaaggagcac gggccggagg atatagtggt
     3001 ggccatcgct ggaaataaga acgatctggg tgacatcagg gaagttccaa ctaaggaggc
     3061 gaaagaattt gcagagtcga ttgcagccat atttatggag accagtgcca gaaatgctgt
     3121 aaacatagag gagctgttcc aaaaaatcag tcgacagatc cctccactgg agaacactga
     3181 tgcagacagc cacgactcct tcaagctgac ccgacagcct cctccagcca ccaaacgctg
     3241 ctgttagaat cgaattcccg cggccgccat ggcggccggg agcatgcgac gtcgggccca
     3301 attcgcccta tagtgagtcg tattacaatt cactggccgt cgttttacaa cgtcgtgact
     3361 gggaaaaccc tggcgttacc caacttaatc gccttgcagc acatccccct ttcgccagct
     3421 ggcgtaatag cgaagaggcc cgcaccgatc gcccttccca acagttgcgc agcctgaatg
     3481 gcgaatggac gcgccctgta gcggcgcatt aagcgcggcg ggtgtggtgg ttacgcgcag
     3541 cgtgaccgct acacttgcca gcgccctagc gcccgctcct ttcgctttct tcccttcctt
     3601 tc
//
