LOCUS       sequence-176583-        3704 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Can be used for in situ hybridisation or other purposes.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3704)
  AUTHORS   Hall TE, Martel N, Lo HP, Xiong Z, Parton RG
  TITLE     A plasmid library of full-length zebrafish rab proteins for in vivo 
            cell biology.
  JOURNAL   Cell Logist. 2017 Mar 1;7(1):e1301151. doi: 
            10.1080/21592799.2017.1301151. eCollection 2017.
  PUBMED    28396820
REFERENCE   2  (bases 1 to 3704)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell 
            Logist."; date: "2017-03-1"; volume: "7(1):e1301151. doi"; pages: " 
            10.1080/21592799.2017.1301151. eCollection 2017"
COMMENT     Sequence Label: Rab3ab cds in pGem-T Easy
FEATURES             Location/Qualifiers
     source          1..3704
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     112..131
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     365..382
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    499..520
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(535..540,541..558,559..565)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    573..589
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     578..600
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     597..613
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     597..613
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     promoter        631..649
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     631..648
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     regulatory      1432..1441
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     regulatory      1436..1445
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     primer_bind     complement(1475..1494)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(1476..1494)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(1501..1518)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(1501..1517)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(1510..1532)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      1658..2113
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(1745..1764)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     1955..1976
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        2191..2295
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(2296..2364,2365..3156)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(2514..2533)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      join(3327..3704,1..211)
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
ORIGIN
        1 cctacagcgt gagctatgag aaagcgccac gcttcccgaa gggagaaagg cggacaggta
       61 tccggtaagc ggcagggtcg gaacaggaga gcgcacgagg gagcttccag ggggaaacgc
      121 ctggtatctt tatagtcctg tcgggtttcg ccacctctga cttgagcgtc gatttttgtg
      181 atgctcgtca ggggggcgga gcctatggaa aaacgccagc aacgcggcct ttttacggtt
      241 cctggccttt tgctggcctt ttgctcacat gttctttcct gcgttatccc ctgattctgt
      301 ggataaccgt attaccgcct ttgagtgagc tgataccgct cgccgcagcc gaacgaccga
      361 gcgcagcgag tcagtgagcg aggaagcgga agagcgccca atacgcaaac cgcctctccc
      421 cgcgcgttgg ccgattcatt aatgcagctg gcacgacagg tttcccgact ggaaagcggg
      481 cagtgagcgc aacgcaatta atgtgagtta gctcactcat taggcacccc aggctttaca
      541 ctttatgctt ccggctcgta tgttgtgtgg aattgtgagc ggataacaat ttcacacagg
      601 aaacagctat gaccatgatt acgccaagct atttaggtga cactatagaa tactcaagct
      661 atgcatccaa cgcgttggga gctctcccat atggtcgacc tgcaggcggc cgcgaattca
      721 ctagtgatta gctgtgggat tttcagcagg cgcagtcccg gtgcggggcg gcgggctgct
      781 ctgtgagctg cggcccttgt ttggctcccg tcactgcagg atcggcagca tccaggctct
      841 ctgacatctt ctcgcagatt atgtccacca accgctcgaa ggtttgttta acattaatgt
      901 tgtccttggc actggcttcg aaatattcaa aaccaagatg ctcagagagc tgtctgcccc
      961 tttcagaagc cacgaccctc tcatcctcca tgtcacactt gttgcctact agaagcacct
     1021 gagcgttatc ccatgagtat gtcttaatct gtgtggacca gtcctgcact gcagcgaaag
     1081 actcctcatt ggtaatgtcg tacatgagga tgaatcccat ggctccacgg taataagcgg
     1141 tggtgatggt tctgtaacgc tcctgccccg ctgtgtccca gatttgcagc tttatcctct
     1201 tgtcgttcct gtagatggtc ttcaccttga agtcaatgcc gacggtgctt acgaacgctg
     1261 gcgtgaagga gtcatctgca tagcgaaaca gaaagctggt cttcccaacg ctgctattcc
     1321 cgatgattag gattttgaac atgtaatcaa agttctggtc agaagactcc ttttgtccat
     1381 aacggttgtc tgtagctgaa gccatcgtta aaagccaatc gaattcccgc ggccgccatg
     1441 gcggccggga gcatgcgacg tcgggcccaa ttcgccctat agtgagtcgt attacaattc
     1501 actggccgtc gttttacaac gtcgtgactg ggaaaaccct ggcgttaccc aacttaatcg
     1561 ccttgcagca catccccctt tcgccagctg gcgtaatagc gaagaggccc gcaccgatcg
     1621 cccttcccaa cagttgcgca gcctgaatgg cgaatggacg cgccctgtag cggcgcatta
     1681 agcgcggcgg gtgtggtggt tacgcgcagc gtgaccgcta cacttgccag cgccctagcg
     1741 cccgctcctt tcgctttctt cccttccttt ctcgccacgt tcgccggctt tccccgtcaa
     1801 gctctaaatc gggggctccc tttagggttc cgatttagtg ctttacggca cctcgacccc
     1861 aaaaaacttg attagggtga tggttcacgt agtgggccat cgccctgata gacggttttt
     1921 cgccctttga cgttggagtc cacgttcttt aatagtggac tcttgttcca aactggaaca
     1981 acactcaacc ctatctcggt ctattctttt gatttataag ggattttgcc gatttcggcc
     2041 tattggttaa aaaatgagct gatttaacaa aaatttaacg cgaattttaa caaaatatta
     2101 acgcttacaa tttcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc
     2161 gcatcaggtg gcacttttcg gggaaatgtg cgcggaaccc ctatttgttt atttttctaa
     2221 atacattcaa atatgtatcc gctcatgaga caataaccct gataaatgct tcaataatat
     2281 tgaaaaagga agagtatgag tattcaacat ttccgtgtcg cccttattcc cttttttgcg
     2341 gcattttgcc ttcctgtttt tgctcaccca gaaacgctgg tgaaagtaaa agatgctgaa
     2401 gatcagttgg gtgcacgagt gggttacatc gaactggatc tcaacagcgg taagatcctt
     2461 gagagttttc gccccgaaga acgttttcca atgatgagca cttttaaagt tctgctatgt
     2521 ggcgcggtat tatcccgtat tgacgccggg caagagcaac tcggtcgccg catacactat
     2581 tctcagaatg acttggttga gtactcacca gtcacagaaa agcatcttac ggatggcatg
     2641 acagtaagag aattatgcag tgctgccata accatgagtg ataacactgc ggccaactta
     2701 cttctgacaa cgatcggagg accgaaggag ctaaccgctt ttttgcacaa catgggggat
     2761 catgtaactc gccttgatcg ttgggaaccg gagctgaatg aagccatacc aaacgacgag
     2821 cgtgacacca cgatgcctgt agcaatggca acaacgttgc gcaaactatt aactggcgaa
     2881 ctacttactc tagcttcccg gcaacaatta atagactgga tggaggcgga taaagttgca
     2941 ggaccacttc tgcgctcggc ccttccggct ggctggttta ttgctgataa atctggagcc
     3001 ggtgagcgtg ggtctcgcgg tatcattgca gcactggggc cagatggtaa gccctcccgt
     3061 atcgtagtta tctacacgac ggggagtcag gcaactatgg atgaacgaaa tagacagatc
     3121 gctgagatag gtgcctcact gattaagcat tggtaactgt cagaccaagt ttactcatat
     3181 atactttaga ttgatttaaa acttcatttt taatttaaaa ggatctaggt gaagatcctt
     3241 tttgataatc tcatgaccaa aatcccttaa cgtgagtttt cgttccactg agcgtcagac
     3301 cccgtagaaa agatcaaagg atcttcttga gatccttttt ttctgcgcgt aatctgctgc
     3361 ttgcaaacaa aaaaaccacc gctaccagcg gtggtttgtt tgccggatca agagctacca
     3421 actctttttc cgaaggtaac tggcttcagc agagcgcaga taccaaatac tgttcttcta
     3481 gtgtagccgt agttaggcca ccacttcaag aactctgtag caccgcctac atacctcgct
     3541 ctgctaatcc tgttaccagt ggctgctgcc agtggcgata agtcgtgtct taccgggttg
     3601 gactcaagac gatagttacc ggataaggcg cagcggtcgg gctgaacggg gggttcgtgc
     3661 acacagccca gcttggagcg aacgacctac accgaactga gata
//
