LOCUS       sequence-176603-       10187 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  retroviral vector, conditional expression membrane Tdtomato and 
            Kif5Cdelta560.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 10187)
  AUTHORS   Lim L, Pakan JMP, Selten MM, Marques-Smith A, Llorca A, Bae SE, 
            Rochefort NL, Marin O
  TITLE     Optimization of interneuron function by direct coupling of cell 
            migration and axonal targeting.
  JOURNAL   Nat Neurosci. 2018 Jun 18. pii: 10.1038/s41593-018-0162-9. doi: 
            10.1038/s41593-018-0162-9.
  PUBMED    29915195
REFERENCE   2  (bases 1 to 10187)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Neurosci. 2018 Jun 18. pii: 10.1038/s41593-018-0162-9. doi: 
            10.1038/s41593-018-0162-9."
COMMENT     Sequence Label: RV-cag-Dio-Kif5c-2A-tdt
FEATURES             Location/Qualifiers
     source          1..10187
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     enhancer        162..541
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     CDS             complement(555..971)
                     /label=gag (truncated)
                     /note="truncated Moloney murine leukemia virus (MMLV) gag 
                     gene lacking the start codon"
     primer_bind     complement(571..587)
                     /label=pBABE 5'
                     /note="Psi packaging signal, forward primer for pBABE 
                     vectors"
     primer_bind     complement(603..625)
                     /label=pLXSN 5'
                     /note="Murine stem cell virus, forward primer. Also called 
                     MSCV"
     misc_feature    1172..1371
                     /label=MMLV Psi
                     /note="packaging signal of Moloney murine leukemia virus 
                     (MMLV)"
     LTR             1434..1609
                     /label=5' LTR (truncated)
                     /note="truncated long terminal repeat from Moloney murine 
                     sarcoma virus"
     promoter        complement(1610..1813)
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     complement(1639..1659)
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     enhancer        1814..2193
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     CDS             join(2345..2413,2414..3205)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(2563..2582)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      3365..3953
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     3854..3873
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     4107..4124
                     /label=L4440
                     /note="L4440 vector, forward primer"
     misc_feature    4995..5583
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     CDS             5101..5112
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     5510..5530
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     protein_bind    complement(5593..5626)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     protein_bind    complement(5677..5710)
                     /label=lox2272
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (AAGTATCC) (Shaw et al., 2021). lox2272 sites are 
                     compatible with each other, but incompatible with loxP or 
                     loxN sites (Lee and Saito, 1988)."
     CDS             6600..6611
                     /codon_start=1
                     /product="WELQut protease recognition and cleavage site"
                     /label=WELQut site
                     /translation="WELQ"
     regulatory      7411..7420
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(7417..7419,7420..7422,7423..8133)
                     /codon_start=1
                     /product="enhanced YFP"
                     /label=EYFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTFGYGLQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQSTPIGDGPVLLPDNHYLSYQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(7462..7483)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(7723..7742)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     8070..8091
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     CDS             8167..8223
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
     CDS             8293..9723
                     /codon_start=1
                     /product="tandem dimeric (pseudo-monomeric) derivative of 
                     DsRed (Shaner et al., 2004)"
                     /label=tdTomato
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEVIKEFMRFKVRMEGSMNGHEFEIEGEGEGRPYEGTQTA
                     KLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYKKLSFPEGFKWERVMNFEDGG
                     LVTVTQDSSLQDGTLIYKVKMRGTNFPPDGPVMQKKTMGWEASTERLYPRDGVLKGEIH
                     QALKLKDGGHYLVEFKTIYMAKKPVQLPGYYYVDTKLDITSHNEDYTIVEQYERSEGRH
                     HLFLGHGTGSTGSGSSGTASSEDNNMAVIKEFMRFKVRMEGSMNGHEFEIEGEGEGRPY
                     EGTQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYKKLSFPEGFKWERVM
                     NFEDGGLVTVTQDSSLQDGTLIYKVKMRGTNFPPDGPVMQKKTMGWEASTERLYPRDGV
                     LKGEIHQALKLKDGGHYLVEFKTIYMAKKPVQLPGYYYVDTKLDITSHNEDYTIVEQYE
                     RSEGRHHLFLYGMDELYK"
     protein_bind    9732..9765
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     protein_bind    9816..9849
                     /label=lox2272
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (AAGTATCC) (Shaw et al., 2021). lox2272 sites are 
                     compatible with each other, but incompatible with loxP or 
                     loxN sites (Lee and Saito, 1988)."
     primer_bind     complement(9892..9911)
                     /label=pCAG-F
                     /note="Rabbit beta-globin intron, for pCAG plasmids, 
                     forward primer"
     primer_bind     complement(9973..9995)
                     /label=pCAGGS-5
                     /note="Chimeric intron in CAG promoter, forward primer"
ORIGIN
        1 gccccgcccc tcgccccgcc ccgccccgcc tggcgcgcgc cccccccccc ccccgccccc
       61 atcgctgcac aaaataatta aaaaataaat aaatacaaaa ttgggggtgg ggaggggggg
      121 gagatgggga gagtgaagca gaacgtgggg ctcacctcga ccatggtaat agcgatgact
      181 aatacgtaga tgtactgcca agtaggaaag tcccataagg tcatgtactg ggcataatgc
      241 caggcgggcc atttaccgtc attgacgtca atagggggcg tacttggcat atgatacact
      301 tgatgtactg ccaagtgggc agtttaccgt aaatactcca cccattgacg tcaatggaaa
      361 gtccctattg gcgttactat gggaacatac gtcattattg acgtcaatgg gcgggggtcg
      421 ttgggcggtc agccaggcgg gccatttacc gtaagttatg taacgcggaa ctccatatat
      481 gggctatgaa ctaatgaccc cgtaattgat tactattaat aactagtcaa taatcaatgt
      541 cgacatcgaa ttccggcgcc tagagaagga gtgagggctg gataaaggga ggatcgaggc
      601 ggggtcgaac gaggaggttc aagggggaga gacggggcgg atggaggaag aggaggcgga
      661 ggcttagggt gtacaaaggg cttgacccag ggaggggggt caaaagccaa ggcttcccag
      721 gtcacgatgt aggggacctg gtctgggtgt ccatgcgggc caggtgaaaa gaccttgatc
      781 ttaacctggg tgatgaggtc tcggttaaag gtgccgtctc gcggccatcc gacgttaaag
      841 gttggccatt ctgcagagca gaaggtaacc caacgtctct tcttgacatc taccgactgg
      901 ttgtgagcga tccgctcgac atctttccag tgacctaagg tcaaacttaa gggagtggta
      961 acagtctggc cctaattttc agacaaatac agaaacacag tcagacagag acaacacaga
     1021 acgatgctgc agcgctgcag cagacaagac gcgcggcttc ggttccaaac cgaaagcaaa
     1081 aattcagacg gaggcgggaa ctgttttagg ttctcgtctc ctaccagaac cacatatcct
     1141 gacggggtcg gattccacat cgactccctt cctcaggtcg ggccacaaaa acggccccca
     1201 aagtccctgg gacgtctccc agggttgcgg ccgggtgttc agaactcgtc agttccacca
     1261 cgggtccgcc agatacagag ctagttagct aactagtaca gacgcaggcg cataacatca
     1321 aacatagaca ctagacaatc ggacagacac agataagttg ctggccagct tacctcccgg
     1381 tggtgggtcg gtggtccctg ggcaggggtc tccaaatccc ggacgagccc ccaaatgaaa
     1441 gacccccgtc gtgggtagtc aatcactcag aggagaccct cccaaggaac agcgagacca
     1501 cgattcggat gcaaacagca agaggcttta ttgggaatac gggtacccgg gcgactcagt
     1561 caatcggagg actggcgccc cgagtgaggg gttgtgggct cttttattga gctctgctta
     1621 tatagacctc ccaccgtaca cgcctaccgc ccatttgcgt caatggggcg gagttgttac
     1681 gacattttgg aaagtcccgt tgattttggt gccaaaacaa actcccattg acgtcaatgg
     1741 ggtggagact tggaaatccc cgtgagtcaa accgctatcc acgcccattg atgtactgcc
     1801 aaaaccgcat caccatggta atagcgatga ctaatacgta gatgtactgc caagtaggaa
     1861 agtcccataa ggtcatgtac tgggcataat gccaggcggg ccatttaccg tcattgacgt
     1921 caataggggg cgtacttggc atatgataca cttgatgtac tgccaagtgg gcagtttacc
     1981 gtaaatactc cacccattga cgtcaatgga aagtccctat tggcgttact atgggaacat
     2041 acgtcattat tgacgtcaat gggcgggggt cgttgggcgg tcagccaggc gggccattta
     2101 ccgtaagtta tgtaacgcgg aactccatat atgggctatg aactaatgac cccgtaattg
     2161 attactatta ataactagtc aataatcaat gtcaacatgg cggtaatgtt ggacatgagc
     2221 caatataaat gtacatatta tgatatggat acaacgtatg caatggccaa tagccaatat
     2281 tgatttatgc tatataacca atgaataata tggctaatgg ccggatcatt gaaaaaggaa
     2341 gagtatgagt attcaacatt tccgtgtcgc ccttattccc ttttttgcgg cattttgcct
     2401 tcctgttttt gctcacccag aaacgctggt gaaagtaaaa gatgctgaag atcagttggg
     2461 tgcacgagtg ggttacatcg aactggatct caacagcggt aagatccttg agagttttcg
     2521 ccccgaagaa cgttttccaa tgatgagcac ttttaaagtt ctgctatgtg gcgcggtatt
     2581 atcccgtatt gacgccgggc aagagcaact cggtcgccgc atacactatt ctcagaatga
     2641 cttggttgag tactcaccag tcacagaaaa gcatcttacg gatggcatga cagtaagaga
     2701 attatgcagt gctgccataa ccatgagtga taacactgcg gccaacttac ttctgacaac
     2761 gatcggagga ccgaaggagc taaccgcttt tttgcacaac atgggggatc atgtaactcg
     2821 ccttgatcgt tgggaaccgg agctgaatga agccatacca aacgacgagc gtgacaccac
     2881 gatgcctgta gcaatggcaa caacgttgcg caaactatta actggcgaac tacttactct
     2941 agcttcccgg caacaattaa tagactggat ggaggcggat aaagttgcag gaccacttct
     3001 gcgctcggcc cttccggctg gctggtttat tgctgataaa tctggagccg gtgagcgtgg
     3061 gtctcgcggt atcattgcag cactggggcc agatggtaag ccctcccgta tcgtagttat
     3121 ctacacgacg gggagtcagg caactatgga tgaacgaaat agacagatcg ctgagatagg
     3181 tgcctcactg attaagcatt ggtaactgtc agaccaagtt tactcatata tactttagat
     3241 tgatttgcgg ccgcaaaagg atctaggtga agatcctttt tgataatctc atgaccaaaa
     3301 tcccttaacg tgagttttcg ttccactgag cgtcagaccc cgtagaaaag atcaaaggat
     3361 cttcttgaga tccttttttt ctgcgcgtaa tctgctgctt gcaaacaaaa aaaccaccgc
     3421 taccagcggt ggtttgtttg ccggatcaag agctaccaac tctttttccg aaggtaactg
     3481 gcttcagcag agcgcagata ccaaatactg ttcttctagt gtagccgtag ttaggccacc
     3541 acttcaagaa ctctgtagca ccgcctacat acctcgctct gctaatcctg ttaccagtgg
     3601 ctgctgccag tggcgataag tcgtgtctta ccgggttgga ctcaagacga tagttaccgg
     3661 ataaggcgca gcggtcgggc tgaacggggg gttcgtgcac acagcccagc ttggagcgaa
     3721 cgacctacac cgaactgaga tacctacagc gtgagctatg agaaagcgcc acgcttcccg
     3781 aagggagaaa ggcggacagg tatccggtaa gcggcagggt cggaacagga gagcgcacga
     3841 gggagcttcc agggggaaac gcctggtatc tttatagtcc tgtcgggttt cgccacctct
     3901 gacttgagcg tcgatttttg tgatgctcgt caggggggcg gagcctatgg aaaaacgcca
     3961 gcaacgcggc ctttttacgg ttcctggcct tttgctggcc ttttgctcac atgttctttc
     4021 ctgcgttatc ccctgattct gtggataacc gtattaccgc ctttgagtga gctgataccg
     4081 ctcgccgcag ccgaacgacc gagcgcagcg agtcagtgag cgaggaagcg gaagagcgcc
     4141 caatacgcaa accgcctctc cccgcgcgtt ggccgattca ttaatgcaac tatggccatt
     4201 taatgtaaat acttaagaaa aaaaaccaaa ttaattttga tacatgctgc atgtgaaaga
     4261 cccccgctga cgggtagtca atcactcaga ggagaccctc ccaaggaaca gcgagaccac
     4321 aagtcggatg caaawgmccc ccgctgacgg gtagtcaatc actcagagga gaccctccca
     4381 aggaacagcg agaccacaag tcggatgcaa ctgcaagagg gtttattgga tacacgggta
     4441 cccgggcgac tcagtcaatc ggaggactgg cgccccgagt gaggggttgt gggctctttt
     4501 attgagctcg gggagcagaa gcgcgcgaac agaagcgaga agcgaactga ttggttagtt
     4561 caaataaggc acagggtcat ttcaggtcct tggggcaccc tggaaacatc tgatggttct
     4621 ctagaaactg ctgagggctg gaccgcatct ggggaccatc tgttcttggc cctgagccgg
     4681 ggcaggaact gcttaccaca gatatcctgt ttggcccata ttcagctgtt ccatctgttc
     4741 ttggccctga gccggggcag gaactgctta ccacagatat cctgtttggc ccatattcag
     4801 ctgttccatc tgttcctgac cttgatctga acttctctat tctcagttat gtatttttcc
     4861 atgccttgca aaatggcgtt acttaagcta gcttgccaaa cctacaggtg gggtctttca
     4921 ttcccccctt tttctggaga ctaaataaaa tcttttattt tatcgatgtt aacaagctcg
     4981 agctcgaatt ccaggcgggg aggcggccca aagggagatc cgactcgtct gagggcgaag
     5041 gcgaagacgc ggaagaggcc gcagagccgg cagcaggccg cgggaaggaa ggtccgctgg
     5101 attgagggcc gaagggacgt agcagaagga cgtcccgcgc agaatccagg tggcaacaca
     5161 ggcgagcagc catggaaagg acgtcagctt ccccgacaac accacggaat tgtcagtgcc
     5221 caacagccga gcccctgtcc agcagcgggc aaggcaggcg gcgatgagtt ccgccgtggc
     5281 aatagggagg gggaaagcga aagtcccgga aaggagctga caggtggtgg caatgcccca
     5341 accagtgggg gttgcgtcag caaacacagt gcacaccacg ccacgttgcc tgacaacggg
     5401 ccacaactcc tcataaagag acagcaacca ggatttatac aaggaggaga aaatgaaagc
     5461 catacgggaa gcaatagcat gatacaaagg cattaaagca gcgtatccac atagcgtaaa
     5521 aggagcaaca tagttaagaa taccagtcaa tctttcacaa attttgtaat ccagaggttg
     5581 attggtttaa acataacttc gtatagcata cattatacga agttattctt tgcaccattc
     5641 taaagaataa cagtgataat ttctgggtta aggcaaataa cttcgtatag gatactttat
     5701 acgaagttat cgcggcgcgc catggcggat ccagccgaat gcagcatcaa agtgatgtgc
     5761 cggttccggc ccctcaacga agctgagatc ctccgcgggg acaaattcat ccccaaattc
     5821 aaaggcgagg agacggtggc gatcgggcaa gggaagccgt atgtctttga ccgagttctg
     5881 ccgcccaaca caacccagga gcaggtctac aatgcctgtg cgaagcagat tgtcaaagat
     5941 gtcctcgagg gttacaatgg gacaattttt gcatacgggc agacttcatc aggaaaaact
     6001 cataccatgg aggggaagct gcacgatcct cagcttatgg gtatcatacc caggatcgca
     6061 cacgatattt tcgatcacat ctattccatg gatgtgaact tggagtttca tatcaaggtt
     6121 tcctattttg agatctactt ggacaaaata agggacttgc ttgacgtgtc caagaccaac
     6181 ttggctgttc atgaagacaa aaacagagtc ccctatgtaa aggggtgcac cgagaggttt
     6241 gtgtcaagcc cggaggaggt catggatgtg atcgatgagg gcaaagcaaa ccgacatgtg
     6301 gctgtgacaa acatgaacga acacagctcc aggagtcaca gtatcttcct gattaacatt
     6361 aaacaagaga atgtggagac tgaaaaaaaa ctcagcggga agctgtattt ggttgatttg
     6421 gctgggagtg aaaaggtcag caaaactggt gccgagggag ctgttcttga tgaagctaaa
     6481 aatatcaaca agtctttgtc tgctcttgga aatgtgatct ctgccttggc agaagggaca
     6541 aaaacacatg tgccataccg ggacagcaag atgactcgga ttctccagga ctctctgggt
     6601 gggaactgca ggaccaccat tgtcatttgc tgttctcctt cagtcttcaa tgaggctgag
     6661 accaagtcca cgctgatgtt tggacagaga gcaaagacca tcaagaatac ggtctctgtg
     6721 aacttggaac taacagcaga agaatggaag aagaaatatg aaaaagagaa agagaagaat
     6781 aaggccctga agagtgtcat ccagcatctg gaggtggagc tgaacaggtg gaggaacggg
     6841 gaagctgtgc ctgaggatga acagatcagt gccaaggacc agaagaacct ggagccctgc
     6901 gacaacacac ccatcataga caacatcaca cctgtggttg acggcatctc tgctgagaaa
     6961 gagaagtatg acgaggaaat caccagtctg taccgacagc tggatgacaa ggacgatgaa
     7021 attaaccagc agagccagct ggctgaaaag ctgaagcaac agatgttgga tcaggacgag
     7081 cttctagctt ccaccagaag ggactacgag aaaatccagg aggagctgac ccgcctccag
     7141 attgaaaacg aggcggctaa agacgaggtg aaggaggtcc tccaggccct ggaggagctg
     7201 gctgtcaatt acgaccagaa gtcacaggaa gtggaggaca agacccgggc caacgagcaa
     7261 ctgaccgatg agctggccca gaaaacgacg acactgacaa ccacccagcg ggagctgagt
     7321 cagctacagg agcttagcaa ccaccagaag aagagggcca cggagatcct gaacctgctt
     7381 ctcaaggatc tgggcgagat atcaccggtc gccaccatgg tgagcaaggg cgaggagctg
     7441 ttcaccgggg tggtgcccat cctggtcgag ctggacggcg acgtaaacgg ccacaagttc
     7501 agcgtgtccg gcgagggcga gggcgatgcc acctacggca agctgaccct gaagttcatc
     7561 tgcaccaccg gcaagctgcc cgtgccctgg cccaccctcg tgaccacctt cggctacggc
     7621 ctgcagtgct tcgcccgcta ccccgaccac atgaagcagc acgacttctt caagtccgcc
     7681 atgcccgaag gctacgtcca ggagcgcacc atcttcttca aggacgacgg caactacaag
     7741 acccgcgccg aggtgaagtt cgagggcgac accctggtga accgcatcga gctgaagggc
     7801 atcgacttca aggaggacgg caacatcctg gggcacaagc tggagtacaa ctacaacagc
     7861 cacaacgtct atatcatggc cgacaagcag aagaacggca tcaaggtgaa cttcaagatc
     7921 cgccacaaca tcgaggacgg cagcgtgcag ctcgccgacc actaccagca gagcaccccc
     7981 atcggcgacg gccccgtgct gctgcccgac aaccactacc tgagctacca gtccgccctg
     8041 agcaaagacc ccaacgagaa gcgcgatcac atggtcctgc tggagttcgt gaccgccgcc
     8101 gggatcactc tcggcatgga cgagctgtac aagtacgtcg acggtaccgc gggcccggga
     8161 tccggcgcca caaacttctc tctgctaaag caagcaggtg atgttgaaga aaaccccggg
     8221 cctacacgcg ccatgctgtg ctgtatgaga agaaccaaac aggttgaaaa gaatgatgag
     8281 gaccaaaaga tcatggtgag caagggcgag gaggtcatca aagagttcat gcgcttcaag
     8341 gtgcgcatgg agggctccat gaacggccac gagttcgaga tcgagggcga gggcgagggc
     8401 cgcccctacg agggcaccca gaccgccaag ctgaaggtga ccaagggcgg ccccctgccc
     8461 ttcgcctggg acatcctgtc cccccagttc atgtacggct ccaaggcgta cgtgaagcac
     8521 cccgccgaca tccccgatta caagaagctg tccttccccg agggcttcaa gtgggagcgc
     8581 gtgatgaact tcgaggacgg cggtctggtg accgtgaccc aggactcctc cctgcaggac
     8641 ggcacgctga tctacaaggt gaagatgcgc ggcaccaact tcccccccga cggccccgta
     8701 atgcagaaga agaccatggg ctgggaggcc tccaccgagc gcctgtaccc ccgcgacggc
     8761 gtgctgaagg gcgagatcca ccaggccctg aagctgaagg acggcggcca ctacctggtg
     8821 gagttcaaga ccatctacat ggccaagaag cccgtgcaac tgcccggcta ctactacgtg
     8881 gacaccaagc tggacatcac ctcccacaac gaggactaca ccatcgtgga acagtacgag
     8941 cgctccgagg gccgccacca cctgttcctg gggcatggca ccggcagcac cggcagcggc
     9001 agctccggca ccgcctcctc cgaggacaac aacatggccg tcatcaaaga gttcatgcgc
     9061 ttcaaggtgc gcatggaggg ctccatgaac ggccacgagt tcgagatcga gggcgagggc
     9121 gagggccgcc cctacgaggg cacccagacc gccaagctga aggtgaccaa gggcggcccc
     9181 ctgcccttcg cctgggacat cctgtccccc cagttcatgt acggctccaa ggcgtacgtg
     9241 aagcaccccg ccgacatccc cgattacaag aagctgtcct tccccgaggg cttcaagtgg
     9301 gagcgcgtga tgaacttcga ggacggcggt ctggtgaccg tgacccagga ctcctccctg
     9361 caggacggca cgctgatcta caaggtgaag atgcgcggca ccaacttccc ccccgacggc
     9421 cccgtaatgc agaagaagac catgggctgg gaggcctcca ccgagcgcct gtacccccgc
     9481 gacggcgtgc tgaagggcga gatccaccag gccctgaagc tgaaggacgg cggccactac
     9541 ctggtggagt tcaagaccat ctacatggcc aagaagcccg tgcaactgcc cggctactac
     9601 tacgtggaca ccaagctgga catcacctcc cacaacgagg actacaccat cgtggaacag
     9661 tacgagcgct ccgagggccg ccaccacctg ttcctgtacg gcatggacga gctgtacaag
     9721 taattaatta aataacttcg tataatgtat gctatacgaa gttattaaga ggtttcatat
     9781 tgctaatagc agctacaatc cagctaccat tctgcataac ttcgtataaa gtatcctata
     9841 cgaagttatt ccggatccgc tagcgaattc tttgccaaaa tgatgagaca gcacaataac
     9901 cagcacgttg cccaggagct gtaggaaaaa gaagaaggca tgaacatggt tagcagaggc
     9961 tctagagccg ccggtcacac gccagaagcc gaaccccgcc ctgccccgtc ccccccgaag
    10021 gcagccgtcc ccccgcggac agccccgagg ctggagaggg agaaggggac ggcggcgcgg
    10081 cgacgcacga aggccctccc cgcccatttc cttcctgccg gcgccgcacc gcttcgcccc
    10141 gcgcccgcta gagggggtgc ggcggcgcct cccagatttc ggctccg
//
