LOCUS       sequence-176638-        3951 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3951)
  AUTHORS   Johannessen CM, Boehm JS, Kim SY, Thomas SR, Wardwell L, Johnson LA,
            Emery CM, Stransky N, Cogdill AP, Barretina J, Caponigro G, 
            Hieronymus H, Murray RR, Salehi-Ashtiani K, Hill DE, Vidal M, Zhao 
            JJ, Yang X, Alkan O, Kim S, Harris JL, Wilson CJ, Myer VE, Finan PM,
            Root DE, Roberts TM, Golub T, Flaherty KT, Dummer R, Weber BL, 
            Sellers WR, Schlegel R, Wargo JA, Hahn WC, Garraway LA
  TITLE     COT drives resistance to RAF inhibition through MAP kinase pathway 
            reactivation.
  JOURNAL   Nature. 2010 Dec 16;468(7326):968-72. doi: 10.1038/nature09627
  PUBMED    21107320
REFERENCE   2  (bases 1 to 3951)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10"; journalName: 
            "Nature"; date: "2010-12-16- 16"; volume: "468"; issue: "7326"; 
            pages: "968-72"
COMMENT     Sequence Label: pDONR223-ADRBK2
FEATURES             Location/Qualifiers
     source          1..3951
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             124..915
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     rep_origin      1008..1596
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     1497..1516
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     1750..1767
                     /label=L4440
                     /note="L4440 vector, forward primer"
     terminator      complement(1926..1953)
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     terminator      complement(2045..2131)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     primer_bind     2180..2202
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     2194..2211
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     2195..2211
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    2227..2326
                     /gene="mutant version of attL"
                     /label=attL1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     protein_bind    complement(3488..3587)
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(3604..3623)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(3605..3623)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(3628..3644)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(3628..3644)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
ORIGIN
        1 gtacagtcta tgcctcgggc atccaagcag caagcgcgtt acgccgtggg tcgatgtttg
       61 atgttatgga gcagcaacga tgttacgcag cagggcagtc gccctaaaac aaagttaaac
      121 attatgaggg aagcggtgat cgccgaagta tcgactcaac tatcagaggt agttggcgtc
      181 atcgagcgcc atctcgaacc gacgttgctg gccgtacatt tgtacggctc cgcagtggat
      241 ggcggcctga agccacacag tgatattgat ttgctggtta cggtgaccgt aaggcttgat
      301 gaaacaacgc ggcgagcttt gatcaacgac cttttggaaa cttcggcttc ccctggagag
      361 agcgagattc tccgcgctgt agaagtcacc attgttgtgc acgacgacat cattccgtgg
      421 cgttatccag ctaagcgcga actgcaattt ggagaatggc agcgcaatga cattcttgca
      481 ggtatcttcg agccagccac gatcgacatt gatctggcta tcttgctgac aaaagcaaga
      541 gaacatagcg ttgccttggt aggtccagcg gcggaggaac tctttgatcc ggttcctgaa
      601 caggatctat ttgaggcgct aaatgaaacc ttaacgctat ggaactcgcc gcccgactgg
      661 gctggcgatg agcgaaatgt agtgcttacg ttgtcccgca tttggtacag cgcagtaacc
      721 ggcaaaatcg cgccgaagga tgtcgctgcc gactgggcaa tggagcgcct gccggcccag
      781 tatcagcccg tcatacttga agctagacag gcttatcttg gacaagaaga agatcgcttg
      841 gcctcgcgcg cagatcagtt ggaagaattt gtccactacg tgaaaggcga gatcaccaag
      901 gtagtcggca aataaccctc gagccaccca tgaccaaaat cccttaacgt gagttacgcg
      961 tcgttccact gagcgtcaga ccccgtagaa aagatcaaag gatcttcttg agatcctttt
     1021 tttctgcgcg taatctgctg cttgcaaaca aaaaaaccac cgctaccagc ggtggtttgt
     1081 ttgccggatc aagagctacc aactcttttt ccgaaggtaa ctggcttcag cagagcgcag
     1141 ataccaaata ctgttcttct agtgtagccg tagttaggcc accacttcaa gaactctgta
     1201 gcaccgccta catacctcgc tctgctaatc ctgttaccag tggctgctgc cagtggcgat
     1261 aagtcgtgtc ttaccgggtt ggactcaaga cgatagttac cggataaggc gcagcggtcg
     1321 ggctgaacgg ggggttcgtg cacacagccc agcttggagc gaacgaccta caccgaactg
     1381 agatacctac agcgtgagct atgagaaagc gccacgcttc ccgaagggag aaaggcggac
     1441 aggtatccgg taagcggcag ggtcggaaca ggagagcgca cgagggagct tccaggggga
     1501 aacgcctggt atctttatag tcctgtcggg tttcgccacc tctgacttga gcgtcgattt
     1561 ttgtgatgct cgtcaggggg gcggagccta tggaaaaacg ccagcaacgc ggccttttta
     1621 cggttcctgg ccttttgctg gccttttgct cacatgttct ttcctgcgtt atcccctgat
     1681 tctgtggata accgtattac cgcctttgag tgagctgata ccgctcgccg cagccgaacg
     1741 accgagcgca gcgagtcagt gagcgaggaa gcggaagagc gcccaatacg caaaccgcct
     1801 ctccccgcgc gttggccgat tcattaatgc agctggcacg acaggtttcc cgactggaaa
     1861 gcgggcagtg agcgcaacgc aattaatacg cgtaccgcta gccaggaaga gtttgtagaa
     1921 acgcaaaaag gccatccgtc aggatggcct tctgcttagt ttgatgcctg gcagtttatg
     1981 gcgggcgtcc tgcccgccac cctccgggcc gttgcttcac aacgttcaaa tccgctcccg
     2041 gcggatttgt cctactcagg agagcgttca ccgacaaaca acagataaaa cgaaaggccc
     2101 agtcttccga ctgagccttt cgttttattt gatgcctggc agttccctac tctcgcgtta
     2161 acgctagcat ggatgttttc ccagtcacga cgttgtaaaa cgacggccag tcttaagctc
     2221 gggccccaaa taatgatttt attttgactg atagtgacct gttcgttgca acaaattgat
     2281 gagcaatgct tttttataat gccaactttg tacaaaaaag ttggcatggc ggacctggag
     2341 gctgtgctgg ccgatgtcag ttacctgatg gccatggaga agagcaaggc gaccccggcc
     2401 gcccgcgcca gcaagaggat cgtcctgccg gagcccagta tccggagtgt gatgcagaag
     2461 taccttgcag agagaaatga aataaccttt gacaagattt tcaatcagaa aattggtttc
     2521 ttgctattta aagatttttg tttgaatgaa attaatgaag ctgtacctca ggtgaagttt
     2581 tatgaagaga taaaggaata tgaaaaactt gataatgagg aagaccgcct ttgcagaagt
     2641 cgacaaattt atgatgccta catcatgaag gaacttcttt cctgttcaca tcctttctca
     2701 aagcaagctg tagaacacgt acaaagtcat ttatccaaga aacaagtgac atcaactctt
     2761 tttcagccat acatagaaga aatttgtgaa agccttcgag gtgacatttt tcaaaaattt
     2821 atggaaagtg acaagttcac tagattttgt cagtggaaaa acgttgaatt aaatatccat
     2881 ttgaccatga atgagttcag tgtgcatagg attattggac gaggaggatt cggggaagtt
     2941 tatggttgca ggaaagcaga cactggaaaa atgtatgcaa tgaaatgctt agataagaag
     3001 aggatcaaaa tgaaacaagg agaaacatta gccttaaatg aaagaatcat gttgtctctt
     3061 gtcagcacag gagactgtcc tttcattgta tgtatgacct atgccttcca taccccagat
     3121 aaactctgct tcatcctgga tctgatgaac gggggcgatt tgcactacca cctttcacaa
     3181 cacggtgtgt tctctgagaa ggagatgcgg ttttatgcca ctgaaatcat tctgggtctg
     3241 gaacacatgc acaatcggtt tgttgtctac agagatttga agccagcaaa tattctcttg
     3301 gatgaacatg gacacgcaag aatatcagat cttggtcttg cctgcgattt ttccaaaaag
     3361 aagcctcatg cgagtgttgg cacccatggg tacatggctc ccgaggtgct gcagaagggg
     3421 acggcctatg acagcagtgc cgactggttc tccctgggct gcatgctttt caaacttctg
     3481 agaggatgcc caactttctt gtacaaagtt ggcattataa gaaagcattg cttatcaatt
     3541 tgttgcaacg aacaggtcac tatcagtcaa aataaaatca ttatttgcca tccagctgat
     3601 atcccctata gtgagtcgta ttacatggtc atagctgttt cctggcagct ctggcccgtg
     3661 tctcaaaatc tctgatgtta cattgcacaa gataaaaata tatcatcatg cctcctctag
     3721 accagccagg acagaaatgc ctcgacttcg ctgctaccca aggttgccgg gtgacgcaca
     3781 ccgtggaaac ggatgaaggc acgaacccag tggacataag cctgttcggt tcgtaagctg
     3841 taatgcaagt agcgtatgcg ctcacgcaac tggtccagaa ccttgaccga acgcagcggt
     3901 ggtaacggcg cagtggcggt tttcatggct tgttatgact gtttttttgg g
//
