LOCUS       sequence-176639-        3689 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Can be used for in situ hybridisation or other purposes.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3689)
  AUTHORS   Hall TE, Martel N, Lo HP, Xiong Z, Parton RG
  TITLE     A plasmid library of full-length zebrafish rab proteins for in vivo 
            cell biology.
  JOURNAL   Cell Logist. 2017 Mar 1;7(1):e1301151. doi: 
            10.1080/21592799.2017.1301151. eCollection 2017.
  PUBMED    28396820
REFERENCE   2  (bases 1 to 3689)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell 
            Logist."; date: "2017-03-1"; volume: "7(1):e1301151. doi"; pages: " 
            10.1080/21592799.2017.1301151. eCollection 2017"
COMMENT     Sequence Label: Rab11a cds in pGem-T Easy
FEATURES             Location/Qualifiers
     source          1..3689
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             complement(join(81..872,873..941))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     704..723
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(942..1046)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      complement(1124..1579)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(1261..1282)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     1473..1492
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     1705..1727
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     1719..1736
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     1720..1736
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     1743..1762
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        1743..1761
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     regulatory      1792..1801
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     regulatory      1796..1805
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     promoter        complement(2573..2591)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(2574..2591)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     complement(2609..2625)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(2609..2625)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(2622..2644)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    2633..2649
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(2657..2663,2664..2681,2682..2687))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    2702..2723
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(2840..2857)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(3011..3599)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(3091..3110)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 atcttcacct agatcctttt aaattaaaaa tgaagtttta aatcaatcta aagtatatat
       61 gagtaaactt ggtctgacag ttaccaatgc ttaatcagtg aggcacctat ctcagcgatc
      121 tgtctatttc gttcatccat agttgcctga ctccccgtcg tgtagataac tacgatacgg
      181 gagggcttac catctggccc cagtgctgca atgataccgc gagacccacg ctcaccggct
      241 ccagatttat cagcaataaa ccagccagcc ggaagggccg agcgcagaag tggtcctgca
      301 actttatccg cctccatcca gtctattaat tgttgccggg aagctagagt aagtagttcg
      361 ccagttaata gtttgcgcaa cgttgttgcc attgctacag gcatcgtggt gtcacgctcg
      421 tcgtttggta tggcttcatt cagctccggt tcccaacgat caaggcgagt tacatgatcc
      481 cccatgttgt gcaaaaaagc ggttagctcc ttcggtcctc cgatcgttgt cagaagtaag
      541 ttggccgcag tgttatcact catggttatg gcagcactgc ataattctct tactgtcatg
      601 ccatccgtaa gatgcttttc tgtgactggt gagtactcaa ccaagtcatt ctgagaatag
      661 tgtatgcggc gaccgagttg ctcttgcccg gcgtcaatac gggataatac cgcgccacat
      721 agcagaactt taaaagtgct catcattgga aaacgttctt cggggcgaaa actctcaagg
      781 atcttaccgc tgttgagatc cagttcgatg taacccactc gtgcacccaa ctgatcttca
      841 gcatctttta ctttcaccag cgtttctggg tgagcaaaaa caggaaggca aaatgccgca
      901 aaaaagggaa taagggcgac acggaaatgt tgaatactca tactcttcct ttttcaatat
      961 tattgaagca tttatcaggg ttattgtctc atgagcggat acatatttga atgtatttag
     1021 aaaaataaac aaataggggt tccgcgcaca tttccccgaa aagtgccacc tgatgcggtg
     1081 tgaaataccg cacagatgcg taaggagaaa ataccgcatc aggaaattgt aagcgttaat
     1141 attttgttaa aattcgcgtt aaatttttgt taaatcagct cattttttaa ccaataggcc
     1201 gaaatcggca aaatccctta taaatcaaaa gaatagaccg agatagggtt gagtgttgtt
     1261 ccagtttgga acaagagtcc actattaaag aacgtggact ccaacgtcaa agggcgaaaa
     1321 accgtctatc agggcgatgg cccactacgt gaaccatcac cctaatcaag ttttttgggg
     1381 tcgaggtgcc gtaaagcact aaatcggaac cctaaaggga gcccccgatt tagagcttga
     1441 cggggaaagc cggcgaacgt ggcgagaaag gaagggaaga aagcgaaagg agcgggcgct
     1501 agggcgctgg caagtgtagc ggtcacgctg cgcgtaacca ccacacccgc cgcgcttaat
     1561 gcgccgctac agggcgcgtc cattcgccat tcaggctgcg caactgttgg gaagggcgat
     1621 cggtgcgggc ctcttcgcta ttacgccagc tggcgaaagg gggatgtgct gcaaggcgat
     1681 taagttgggt aacgccaggg ttttcccagt cacgacgttg taaaacgacg gccagtgaat
     1741 tgtaatacga ctcactatag ggcgaattgg gcccgacgtc gcatgctccc ggccgccatg
     1801 gcggccgcgg gaattcgatt tttgatcgag atggggacac gagacgacga atacgactac
     1861 ttgtttaaag tggtcctaat tggagactct ggtgtgggga agagtaacct gctgtctcgt
     1921 ttcactcgca atgaattcaa cctcgagagc aaaagcacta ttggagtgga gttcgccaca
     1981 cgtagcattc aggtggatgg aaagaccgtc aaggctcaga tctgggacac ggcaggacag
     2041 gagcgctacc gagccatcac ttcagcgtat taccgtggag ctgtgggggc cctcctagtg
     2101 tatgacatcg ccaagcatct gacctatgag aatgtggaac gctggcttaa ggagctccga
     2161 gaccacgcag acagcaacat tgtcatcatg ctggtgggca ataaaagtga cttgcgtcac
     2221 cttcgggctg tgcccactga cgaagcacgt gcatttgcag agaaaaacgg tctgtccttc
     2281 ctagagacct cagctttgga ttccaccaat gtagagactg cttttcggac catcctgact
     2341 gaaatctacc gaatcgtatc ccaaaagcag atgtctgacc gccgagacaa tgacatgtca
     2401 cctagcaaca atgtggtgtc catccaggtg caacctactg agaataaacc aaagatgcag
     2461 tgctgccaga gcatctagcg actcctgtac cgaatcacta gtgaattcgc ggccgcctgc
     2521 aggtcgacca tatgggagag ctcccaacgc gttggatgca tagcttgagt attctatagt
     2581 gtcacctaaa tagcttggcg taatcatggt catagctgtt tcctgtgtga aattgttatc
     2641 cgctcacaat tccacacaac atacgagccg gaagcataaa gtgtaaagcc tggggtgcct
     2701 aatgagtgag ctaactcaca ttaattgcgt tgcgctcact gcccgctttc cagtcgggaa
     2761 acctgtcgtg ccagctgcat taatgaatcg gccaacgcgc ggggagaggc ggtttgcgta
     2821 ttgggcgctc ttccgcttcc tcgctcactg actcgctgcg ctcggtcgtt cggctgcggc
     2881 gagcggtatc agctcactca aaggcggtaa tacggttatc cacagaatca ggggataacg
     2941 caggaaagaa catgtgagca aaaggccagc aaaaggccag gaaccgtaaa aaggccgcgt
     3001 tgctggcgtt tttccatagg ctccgccccc ctgacgagca tcacaaaaat cgacgctcaa
     3061 gtcagaggtg gcgaaacccg acaggactat aaagatacca ggcgtttccc cctggaagct
     3121 ccctcgtgcg ctctcctgtt ccgaccctgc cgcttaccgg atacctgtcc gcctttctcc
     3181 cttcgggaag cgtggcgctt tctcatagct cacgctgtag gtatctcagt tcggtgtagg
     3241 tcgttcgctc caagctgggc tgtgtgcacg aaccccccgt tcagcccgac cgctgcgcct
     3301 tatccggtaa ctatcgtctt gagtccaacc cggtaagaca cgacttatcg ccactggcag
     3361 cagccactgg taacaggatt agcagagcga ggtatgtagg cggtgctaca gagttcttga
     3421 agtggtggcc taactacggc tacactagaa gaacagtatt tggtatctgc gctctgctga
     3481 agccagttac cttcggaaaa agagttggta gctcttgatc cggcaaacaa accaccgctg
     3541 gtagcggtgg tttttttgtt tgcaagcagc agattacgcg cagaaaaaaa ggatctcaag
     3601 aagatccttt gatcttttct acggggtctg acgctcagtg gaacgaaaac tcacgttaag
     3661 ggattttggt catgagatta tcaaaaagg
//
