LOCUS       sequence-176642-        3692 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Can be used for in situ hybridisation or other purposes.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3692)
  AUTHORS   Hall TE, Martel N, Lo HP, Xiong Z, Parton RG
  TITLE     A plasmid library of full-length zebrafish rab proteins for in vivo 
            cell biology.
  JOURNAL   Cell Logist. 2017 Mar 1;7(1):e1301151. doi: 
            10.1080/21592799.2017.1301151. eCollection 2017.
  PUBMED    28396820
REFERENCE   2  (bases 1 to 3692)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell 
            Logist."; date: "2017-03-1"; volume: "7(1):e1301151. doi"; pages: " 
            10.1080/21592799.2017.1301151. eCollection 2017"
COMMENT     Sequence Label: Rab27b cds in pGem-T Easy
FEATURES             Location/Qualifiers
     source          1..3692
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(3..22)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(4..22)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(29..46)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(29..45)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(38..60)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      186..641
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(273..292)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     483..504
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        719..823
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(824..892,893..1684)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(1042..1061)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      1855..2443
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     2344..2363
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     2597..2614
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    2731..2752
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(2767..2772,2773..2790,2791..2797)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    2805..2821
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     2810..2832
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     2829..2845
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     2829..2845
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     promoter        2863..2881
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     2863..2880
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     regulatory      3652..3661
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     regulatory      3656..3665
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 cgccctatag tgagtcgtat tacaattcac tggccgtcgt tttacaacgt cgtgactggg
       61 aaaaccctgg cgttacccaa cttaatcgcc ttgcagcaca tccccctttc gccagctggc
      121 gtaatagcga agaggcccgc accgatcgcc cttcccaaca gttgcgcagc ctgaatggcg
      181 aatggacgcg ccctgtagcg gcgcattaag cgcggcgggt gtggtggtta cgcgcagcgt
      241 gaccgctaca cttgccagcg ccctagcgcc cgctcctttc gctttcttcc cttcctttct
      301 cgccacgttc gccggctttc cccgtcaagc tctaaatcgg gggctccctt tagggttccg
      361 atttagtgct ttacggcacc tcgaccccaa aaaacttgat tagggtgatg gttcacgtag
      421 tgggccatcg ccctgataga cggtttttcg ccctttgacg ttggagtcca cgttctttaa
      481 tagtggactc ttgttccaaa ctggaacaac actcaaccct atctcggtct attcttttga
      541 tttataaggg attttgccga tttcggccta ttggttaaaa aatgagctga tttaacaaaa
      601 atttaacgcg aattttaaca aaatattaac gcttacaatt tcctgatgcg gtattttctc
      661 cttacgcatc tgtgcggtat ttcacaccgc atcaggtggc acttttcggg gaaatgtgcg
      721 cggaacccct atttgtttat ttttctaaat acattcaaat atgtatccgc tcatgagaca
      781 ataaccctga taaatgcttc aataatattg aaaaaggaag agtatgagta ttcaacattt
      841 ccgtgtcgcc cttattccct tttttgcggc attttgcctt cctgtttttg ctcacccaga
      901 aacgctggtg aaagtaaaag atgctgaaga tcagttgggt gcacgagtgg gttacatcga
      961 actggatctc aacagcggta agatccttga gagttttcgc cccgaagaac gttttccaat
     1021 gatgagcact tttaaagttc tgctatgtgg cgcggtatta tcccgtattg acgccgggca
     1081 agagcaactc ggtcgccgca tacactattc tcagaatgac ttggttgagt actcaccagt
     1141 cacagaaaag catcttacgg atggcatgac agtaagagaa ttatgcagtg ctgccataac
     1201 catgagtgat aacactgcgg ccaacttact tctgacaacg atcggaggac cgaaggagct
     1261 aaccgctttt ttgcacaaca tgggggatca tgtaactcgc cttgatcgtt gggaaccgga
     1321 gctgaatgaa gccataccaa acgacgagcg tgacaccacg atgcctgtag caatggcaac
     1381 aacgttgcgc aaactattaa ctggcgaact acttactcta gcttcccggc aacaattaat
     1441 agactggatg gaggcggata aagttgcagg accacttctg cgctcggccc ttccggctgg
     1501 ctggtttatt gctgataaat ctggagccgg tgagcgtggg tctcgcggta tcattgcagc
     1561 actggggcca gatggtaagc cctcccgtat cgtagttatc tacacgacgg ggagtcaggc
     1621 aactatggat gaacgaaata gacagatcgc tgagataggt gcctcactga ttaagcattg
     1681 gtaactgtca gaccaagttt actcatatat actttagatt gatttaaaac ttcattttta
     1741 atttaaaagg atctaggtga agatcctttt tgataatctc atgaccaaaa tcccttaacg
     1801 tgagttttcg ttccactgag cgtcagaccc cgtagaaaag atcaaaggat cttcttgaga
     1861 tccttttttt ctgcgcgtaa tctgctgctt gcaaacaaaa aaaccaccgc taccagcggt
     1921 ggtttgtttg ccggatcaag agctaccaac tctttttccg aaggtaactg gcttcagcag
     1981 agcgcagata ccaaatactg ttcttctagt gtagccgtag ttaggccacc acttcaagaa
     2041 ctctgtagca ccgcctacat acctcgctct gctaatcctg ttaccagtgg ctgctgccag
     2101 tggcgataag tcgtgtctta ccgggttgga ctcaagacga tagttaccgg ataaggcgca
     2161 gcggtcgggc tgaacggggg gttcgtgcac acagcccagc ttggagcgaa cgacctacac
     2221 cgaactgaga tacctacagc gtgagctatg agaaagcgcc acgcttcccg aagggagaaa
     2281 ggcggacagg tatccggtaa gcggcagggt cggaacagga gagcgcacga gggagcttcc
     2341 agggggaaac gcctggtatc tttatagtcc tgtcgggttt cgccacctct gacttgagcg
     2401 tcgatttttg tgatgctcgt caggggggcg gagcctatgg aaaaacgcca gcaacgcggc
     2461 ctttttacgg ttcctggcct tttgctggcc ttttgctcac atgttctttc ctgcgttatc
     2521 ccctgattct gtggataacc gtattaccgc ctttgagtga gctgataccg ctcgccgcag
     2581 ccgaacgacc gagcgcagcg agtcagtgag cgaggaagcg gaagagcgcc caatacgcaa
     2641 accgcctctc cccgcgcgtt ggccgattca ttaatgcagc tggcacgaca ggtttcccga
     2701 ctggaaagcg ggcagtgagc gcaacgcaat taatgtgagt tagctcactc attaggcacc
     2761 ccaggcttta cactttatgc ttccggctcg tatgttgtgt ggaattgtga gcggataaca
     2821 atttcacaca ggaaacagct atgaccatga ttacgccaag ctatttaggt gacactatag
     2881 aatactcaag ctatgcatcc aacgcgttgg gagctctccc atatggtcga cctgcaggcg
     2941 gccgcgaatt cactagtgat ttcagcatgc acacttcttc tgttgggcag gtgcggcgct
     3001 cagtttgctg ctgccgtcgc tggtgtttgc gtctgcgctg ggcttctcca cacactgctc
     3061 catccgcttc atcaccagat ccagcagcgt caccacagcc ttgtccacct cagagcccgt
     3121 cgctgcactc gtctcgaagt acgggatgcc gtatttgtct gcgagttctt tcgcttgttt
     3181 ctcctgaact tctctctggt cggctagatc tgctttattt cccaccagca cgatatctgg
     3241 attctcacaa tatgcattag cctggagctg actcatccag ttcctgacgt tgagaaagct
     3301 ctgctggctg gtcagatcaa acatcagcag aaaacccatg gcatccctga agaatgcagt
     3361 ggtcaaactc ctgaacctct cctgtcccgc agtgtcccac agctgcaggt gaaccttaaa
     3421 ggttttcccc gtcgtcccat tggggctgtt agtcgtataa accactctct tttccctgaa
     3481 gtcgatgccc acggtggtga tgaatttggg gttgaacttg ttatctgtgt atcggtagag
     3541 gaaagtggtc ttgccgaccc cggagtcccc caaagccagg agtttgatca gataatcgta
     3601 atctccatcg gtcatagtgc tgctggagct gaacataatc gaattcccgc ggccgccatg
     3661 gcggccggga gcatgcgacg tcgggcccaa tt
//
