LOCUS       sequence-176646-        3671 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Can be used for in situ hybridisation or other purposes.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3671)
  AUTHORS   Hall TE, Martel N, Lo HP, Xiong Z, Parton RG
  TITLE     A plasmid library of full-length zebrafish rab proteins for in vivo 
            cell biology.
  JOURNAL   Cell Logist. 2017 Mar 1;7(1):e1301151. doi: 
            10.1080/21592799.2017.1301151. eCollection 2017.
  PUBMED    28396820
REFERENCE   2  (bases 1 to 3671)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell 
            Logist."; date: "2017-03-1"; volume: "7(1):e1301151. doi"; pages: " 
            10.1080/21592799.2017.1301151. eCollection 2017"
COMMENT     Sequence Label: Rab3b cds in pGem-T Easy
FEATURES             Location/Qualifiers
     source          1..3671
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             complement(join(521..1312,1313..1381))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     1144..1163
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(1382..1486)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      complement(1564..2019)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(1701..1722)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     1913..1932
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     2145..2167
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     2159..2176
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     2160..2176
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     2183..2202
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        2183..2201
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     regulatory      2232..2241
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     regulatory      2236..2245
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     promoter        complement(2995..3013)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(2996..3013)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     complement(3031..3047)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(3031..3047)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(3044..3066)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    3055..3071
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(3079..3085,3086..3103,3104..3109))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    3124..3145
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(3262..3279)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(join(3433..3671,1..350))
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(3513..3532)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 ccaagctggg ctgtgtgcac gaaccccccg ttcagcccga ccgctgcgcc ttatccggta
       61 actatcgtct tgagtccaac ccggtaagac acgacttatc gccactggca gcagccactg
      121 gtaacaggat tagcagagcg aggtatgtag gcggtgctac agagttcttg aagtggtggc
      181 ctaactacgg ctacactaga agaacagtat ttggtatctg cgctctgctg aagccagtta
      241 ccttcggaaa aagagttggt agctcttgat ccggcaaaca aaccaccgct ggtagcggtg
      301 gtttttttgt ttgcaagcag cagattacgc gcagaaaaaa aggatctcaa gaagatcctt
      361 tgatcttttc tacggggtct gacgctcagt ggaacgaaaa ctcacgttaa gggattttgg
      421 tcatgagatt atcaaaaagg atcttcacct agatcctttt aaattaaaaa tgaagtttta
      481 aatcaatcta aagtatatat gagtaaactt ggtctgacag ttaccaatgc ttaatcagtg
      541 aggcacctat ctcagcgatc tgtctatttc gttcatccat agttgcctga ctccccgtcg
      601 tgtagataac tacgatacgg gagggcttac catctggccc cagtgctgca atgataccgc
      661 gagacccacg ctcaccggct ccagatttat cagcaataaa ccagccagcc ggaagggccg
      721 agcgcagaag tggtcctgca actttatccg cctccatcca gtctattaat tgttgccggg
      781 aagctagagt aagtagttcg ccagttaata gtttgcgcaa cgttgttgcc attgctacag
      841 gcatcgtggt gtcacgctcg tcgtttggta tggcttcatt cagctccggt tcccaacgat
      901 caaggcgagt tacatgatcc cccatgttgt gcaaaaaagc ggttagctcc ttcggtcctc
      961 cgatcgttgt cagaagtaag ttggccgcag tgttatcact catggttatg gcagcactgc
     1021 ataattctct tactgtcatg ccatccgtaa gatgcttttc tgtgactggt gagtactcaa
     1081 ccaagtcatt ctgagaatag tgtatgcggc gaccgagttg ctcttgcccg gcgtcaatac
     1141 gggataatac cgcgccacat agcagaactt taaaagtgct catcattgga aaacgttctt
     1201 cggggcgaaa actctcaagg atcttaccgc tgttgagatc cagttcgatg taacccactc
     1261 gtgcacccaa ctgatcttca gcatctttta ctttcaccag cgtttctggg tgagcaaaaa
     1321 caggaaggca aaatgccgca aaaaagggaa taagggcgac acggaaatgt tgaatactca
     1381 tactcttcct ttttcaatat tattgaagca tttatcaggg ttattgtctc atgagcggat
     1441 acatatttga atgtatttag aaaaataaac aaataggggt tccgcgcaca tttccccgaa
     1501 aagtgccacc tgatgcggtg tgaaataccg cacagatgcg taaggagaaa ataccgcatc
     1561 aggaaattgt aagcgttaat attttgttaa aattcgcgtt aaatttttgt taaatcagct
     1621 cattttttaa ccaataggcc gaaatcggca aaatccctta taaatcaaaa gaatagaccg
     1681 agatagggtt gagtgttgtt ccagtttgga acaagagtcc actattaaag aacgtggact
     1741 ccaacgtcaa agggcgaaaa accgtctatc agggcgatgg cccactacgt gaaccatcac
     1801 cctaatcaag ttttttgggg tcgaggtgcc gtaaagcact aaatcggaac cctaaaggga
     1861 gcccccgatt tagagcttga cggggaaagc cggcgaacgt ggcgagaaag gaagggaaga
     1921 aagcgaaagg agcgggcgct agggcgctgg caagtgtagc ggtcacgctg cgcgtaacca
     1981 ccacacccgc cgcgcttaat gcgccgctac agggcgcgtc cattcgccat tcaggctgcg
     2041 caactgttgg gaagggcgat cggtgcgggc ctcttcgcta ttacgccagc tggcgaaagg
     2101 gggatgtgct gcaaggcgat taagttgggt aacgccaggg ttttcccagt cacgacgttg
     2161 taaaacgacg gccagtgaat tgtaatacga ctcactatag ggcgaattgg gcccgacgtc
     2221 gcatgctccc ggccgccatg gcggccgcgg gaattcgatt atggcaaaag cagaccagcg
     2281 ctttggtcag agagatggat ctgatcaaaa cttcgactac atgttcaaac tcctgatcat
     2341 cggcaacagc agcgtaggaa aaaccagctt cttattccga tacgcagacg actccttcag
     2401 cagctcgttt gtcagcacag tcggcatcga cttcaaggtg aagacggtgt acagaaacga
     2461 caagcgagtg aagctccaga tatgggatac ggcgggacag gagcggtata gaactattac
     2521 cacagcttac tatagaggag ccatgggctt catcctcatg tatgacatca ctaatgagga
     2581 gtcctataat gcggtgcagg actgggcaac acaaattaag acttattcat gggataacgc
     2641 tcaggtgata ctagtgggaa acaaatgtga catggatgag gagagggtgg tgccttttga
     2701 gaagggaaaa cacttggctg accagttagg gtttgagtac tatgaagcca gcgccaaaga
     2761 gaacatcaac gtcagacaag tatttgaacg cttagtcgac attatctgcg tgaagatgtc
     2821 agagagagtt gatgtggaac cacccatggt cactggtgcc aaaaccaccc ggctaaccga
     2881 taagccaccc caactacctc agaattgttg ctgaaatcac tagtgaattc gcggccgcct
     2941 gcaggtcgac catatgggag agctcccaac gcgttggatg catagcttga gtattctata
     3001 gtgtcaccta aatagcttgg cgtaatcatg gtcatagctg tttcctgtgt gaaattgtta
     3061 tccgctcaca attccacaca acatacgagc cggaagcata aagtgtaaag cctggggtgc
     3121 ctaatgagtg agctaactca cattaattgc gttgcgctca ctgcccgctt tccagtcggg
     3181 aaacctgtcg tgccagctgc attaatgaat cggccaacgc gcggggagag gcggtttgcg
     3241 tattgggcgc tcttccgctt cctcgctcac tgactcgctg cgctcggtcg ttcggctgcg
     3301 gcgagcggta tcagctcact caaaggcggt aatacggtta tccacagaat caggggataa
     3361 cgcaggaaag aacatgtgag caaaaggcca gcaaaaggcc aggaaccgta aaaaggccgc
     3421 gttgctggcg tttttccata ggctccgccc ccctgacgag catcacaaaa atcgacgctc
     3481 aagtcagagg tggcgaaacc cgacaggact ataaagatac caggcgtttc cccctggaag
     3541 ctccctcgtg cgctctcctg ttccgaccct gccgcttacc ggatacctgt ccgcctttct
     3601 cccttcggga agcgtggcgc tttctcatag ctcacgctgt aggtatctca gttcggtgta
     3661 ggtcgttcgc t
//
