LOCUS       sequence-176655-        3699 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Can be used for in situ hybridisation or other purposes.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3699)
  AUTHORS   Hall TE, Martel N, Lo HP, Xiong Z, Parton RG
  TITLE     A plasmid library of full-length zebrafish rab proteins for in vivo 
            cell biology.
  JOURNAL   Cell Logist. 2017 Mar 1;7(1):e1301151. doi: 
            10.1080/21592799.2017.1301151. eCollection 2017.
  PUBMED    28396820
REFERENCE   2  (bases 1 to 3699)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell 
            Logist."; date: "2017-03-1"; volume: "7(1):e1301151. doi"; pages: " 
            10.1080/21592799.2017.1301151. eCollection 2017"
COMMENT     Sequence Label: Rab11ba cds in pGem-T Easy
FEATURES             Location/Qualifiers
     source          1..3699
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     regulatory      60..69
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     regulatory      64..73
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     primer_bind     complement(103..122)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(104..122)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(129..146)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(129..145)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(138..160)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      286..741
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(373..392)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     583..604
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        819..923
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(924..992,993..1784)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(1142..1161)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      1955..2543
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     2444..2463
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     2697..2714
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    2831..2852
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(2867..2872,2873..2890,2891..2897)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    2905..2921
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     2910..2932
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     2929..2945
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     2929..2945
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     promoter        2963..2981
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     2963..2980
                     /label=SP6
                     /note="SP6 promoter, forward primer"
ORIGIN
        1 tcgtattcgt cgtcacgggt ccccatggtt ggttaaccgc actgaatcga attcccgcgg
       61 ccgccatggc ggccgggagc atgcgacgtc gggcccaatt cgccctatag tgagtcgtat
      121 tacaattcac tggccgtcgt tttacaacgt cgtgactggg aaaaccctgg cgttacccaa
      181 cttaatcgcc ttgcagcaca tccccctttc gccagctggc gtaatagcga agaggcccgc
      241 accgatcgcc cttcccaaca gttgcgcagc ctgaatggcg aatggacgcg ccctgtagcg
      301 gcgcattaag cgcggcgggt gtggtggtta cgcgcagcgt gaccgctaca cttgccagcg
      361 ccctagcgcc cgctcctttc gctttcttcc cttcctttct cgccacgttc gccggctttc
      421 cccgtcaagc tctaaatcgg gggctccctt tagggttccg atttagtgct ttacggcacc
      481 tcgaccccaa aaaacttgat tagggtgatg gttcacgtag tgggccatcg ccctgataga
      541 cggtttttcg ccctttgacg ttggagtcca cgttctttaa tagtggactc ttgttccaaa
      601 ctggaacaac actcaaccct atctcggtct attcttttga tttataaggg attttgccga
      661 tttcggccta ttggttaaaa aatgagctga tttaacaaaa atttaacgcg aattttaaca
      721 aaatattaac gcttacaatt tcctgatgcg gtattttctc cttacgcatc tgtgcggtat
      781 ttcacaccgc atcaggtggc acttttcggg gaaatgtgcg cggaacccct atttgtttat
      841 ttttctaaat acattcaaat atgtatccgc tcatgagaca ataaccctga taaatgcttc
      901 aataatattg aaaaaggaag agtatgagta ttcaacattt ccgtgtcgcc cttattccct
      961 tttttgcggc attttgcctt cctgtttttg ctcacccaga aacgctggtg aaagtaaaag
     1021 atgctgaaga tcagttgggt gcacgagtgg gttacatcga actggatctc aacagcggta
     1081 agatccttga gagttttcgc cccgaagaac gttttccaat gatgagcact tttaaagttc
     1141 tgctatgtgg cgcggtatta tcccgtattg acgccgggca agagcaactc ggtcgccgca
     1201 tacactattc tcagaatgac ttggttgagt actcaccagt cacagaaaag catcttacgg
     1261 atggcatgac agtaagagaa ttatgcagtg ctgccataac catgagtgat aacactgcgg
     1321 ccaacttact tctgacaacg atcggaggac cgaaggagct aaccgctttt ttgcacaaca
     1381 tgggggatca tgtaactcgc cttgatcgtt gggaaccgga gctgaatgaa gccataccaa
     1441 acgacgagcg tgacaccacg atgcctgtag caatggcaac aacgttgcgc aaactattaa
     1501 ctggcgaact acttactcta gcttcccggc aacaattaat agactggatg gaggcggata
     1561 aagttgcagg accacttctg cgctcggccc ttccggctgg ctggtttatt gctgataaat
     1621 ctggagccgg tgagcgtggg tctcgcggta tcattgcagc actggggcca gatggtaagc
     1681 cctcccgtat cgtagttatc tacacgacgg ggagtcaggc aactatggat gaacgaaata
     1741 gacagatcgc tgagataggt gcctcactga ttaagcattg gtaactgtca gaccaagttt
     1801 actcatatat actttagatt gatttaaaac ttcattttta atttaaaagg atctaggtga
     1861 agatcctttt tgataatctc atgaccaaaa tcccttaacg tgagttttcg ttccactgag
     1921 cgtcagaccc cgtagaaaag atcaaaggat cttcttgaga tccttttttt ctgcgcgtaa
     1981 tctgctgctt gcaaacaaaa aaaccaccgc taccagcggt ggtttgtttg ccggatcaag
     2041 agctaccaac tctttttccg aaggtaactg gcttcagcag agcgcagata ccaaatactg
     2101 ttcttctagt gtagccgtag ttaggccacc acttcaagaa ctctgtagca ccgcctacat
     2161 acctcgctct gctaatcctg ttaccagtgg ctgctgccag tggcgataag tcgtgtctta
     2221 ccgggttgga ctcaagacga tagttaccgg ataaggcgca gcggtcgggc tgaacggggg
     2281 gttcgtgcac acagcccagc ttggagcgaa cgacctacac cgaactgaga tacctacagc
     2341 gtgagctatg agaaagcgcc acgcttcccg aagggagaaa ggcggacagg tatccggtaa
     2401 gcggcagggt cggaacagga gagcgcacga gggagcttcc agggggaaac gcctggtatc
     2461 tttatagtcc tgtcgggttt cgccacctct gacttgagcg tcgatttttg tgatgctcgt
     2521 caggggggcg gagcctatgg aaaaacgcca gcaacgcggc ctttttacgg ttcctggcct
     2581 tttgctggcc ttttgctcac atgttctttc ctgcgttatc ccctgattct gtggataacc
     2641 gtattaccgc ctttgagtga gctgataccg ctcgccgcag ccgaacgacc gagcgcagcg
     2701 agtcagtgag cgaggaagcg gaagagcgcc caatacgcaa accgcctctc cccgcgcgtt
     2761 ggccgattca ttaatgcagc tggcacgaca ggtttcccga ctggaaagcg ggcagtgagc
     2821 gcaacgcaat taatgtgagt tagctcactc attaggcacc ccaggcttta cactttatgc
     2881 ttccggctcg tatgttgtgt ggaattgtga gcggataaca atttcacaca ggaaacagct
     2941 atgaccatga ttacgccaag ctatttaggt gacactatag aatactcaag ctatgcatcc
     3001 aacgcgttgg gagctctccc atatggtcga cctgcaggcg gccgcgaatt cactagtgat
     3061 ttgttgggtc acaggttctg acagcactga aatttgttgc cctttaaccc gtcagtagtg
     3121 ggcggcacgc tgatgtccac tacgttattg ccaggagact catcgtgccc gggcctgtcg
     3181 accatctgtt tctgtgacac aatccgatag atttctgtta gtatgttttt aaatgcctcc
     3241 tccacatttg tggagtccaa cgccgatgtt tcaatgaagg agagattgtt cttctctgca
     3301 aaagctctgg cctcatcagt gggcacggcc ctcaggtgcc tcaggtcgct cttgttgccc
     3361 accagcatga tgacgatgtt gttatccgca tgatcccgca gctccttcag ccagcgctcc
     3421 acattctcgt aagtcagatg ttttgcgatg tcgtagacca gaagagctcc aactgcaccc
     3481 cggtagtacg ctgatgttat tgctctgtag cgttcctgtc ctgctgtatc ccagatctga
     3541 gctttaatgg tcttcccgtc cacttggatg ctgcgggttg cgaactccac accaatggtg
     3601 ctcttgctct ccaggttaaa ctcgttccga gtgaaacggg acaacaggtt actcttcccc
     3661 accccagagt ctccaatgag aaccacttta aataaataa
//
