LOCUS       sequence-176684-        5718 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  envelope pseudotyping of lentiviral vectors.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5718)
  AUTHORS   Schoderboeck L, Riad S, Bokor AM, Wicky HE, Strauss M, Bostina M, 
            Oswald MJ, Empson RM, Hughes SM
  TITLE     Chimeric rabies SADB19-VSVg-pseudotyped lentiviral vectors mediate 
            long-range retrograde transduction from the mouse spinal cord.
  JOURNAL   Gene Ther. 2015 May;22(5):357-64. doi: 10.1038/gt.2015.3. Epub 2015 
            Jan 29.
  PUBMED    25630949
REFERENCE   2  (bases 1 to 5718)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1038/gt.2015.3";
            journalName: "Gene Ther"; date: "2015-05"; volume: "22"; issue: "5";
            pages: "357-64"
COMMENT     Sequence Label: N2c/VSVg
FEATURES             Location/Qualifiers
     source          1..5718
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             1495..1527
                     /codon_start=1
                     /product="epitope tag from vesicular stomatitis virus G 
                     protein"
                     /label=VSV-G tag
                     /translation="YTDIEMNRLGK"
     polyA_signal    1626..2020
                     /label=beta-globin poly(A)
                     /note="human beta-globin polyadenylation signal"
     primer_bind     complement(2359..2376)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(2530..3118)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(2610..2629)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(3289..4080,4081..4149))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     3912..3931
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(4150..4254)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(4324..4343)
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     enhancer        4445..4824
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        4825..5028
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     4979..4999
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     5025..5049
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     intron          5162..5637
                     /label=beta-globin intron
                     /note="internally truncated intron from human beta-globin"
ORIGIN
        1 atggttcctc aggttctttt gtttgtactc cttctgggtt tttcgttgtg tttcgggaag
       61 ttccccattt acacgatacc agacgaactt ggtccctgga gccctattga catacaccat
      121 ctcagctgtc caaataacct ggttgtggag gatgaaggat gtaccaacct gtccgagttc
      181 tcctacatgg aactcaaagt gggatacatc tcagccatca aagtgaacgg gttcacttgc
      241 acaggtgttg tgacagaggc agagacctac accaactttg ttggttatgt cacaaccaca
      301 ttcaagagaa agcatttccg ccccacccca gacgcatgta gagccgcgta taactggaag
      361 atggccggtg accccagata tgaagagtcc ctacacaatc cataccccga ctaccactgg
      421 cttcgaactg taagaaccac caaagagtcc ctcattatca tatccccaag tgtgacagat
      481 ttggacccat atgacaaatc ccttcactca agggtcttcc ctggcggaaa gtgctcagga
      541 ataacggtgt cctctaccta ctgctcaact aaccatgatt acaccatttg gatgcccgag
      601 aatccgagac caaggacacc ttgtgacatt tttaccaata gcagagggaa gagagcatcc
      661 aacgggaaca agacttgcgg ctttgtggat gaaagaggcc tgtataagtc tctaaaagga
      721 gcatgcaggc tcaagttatg tggagttctt ggacttagac ttatggatgg aacatgggtc
      781 gcgatgcaaa catcagatga gaccaaatgg tgccctccag atcagttggt gaatttgcac
      841 gactttcgct cagacgagat tgagcatctc gttgtggagg agttagtcaa gaaaagagag
      901 gaatgtctgg acgcattaga gtccatcatg accaccaagt cagtaagttt cagacgtctc
      961 agtcacctga gaaaacttgt cccagggttt ggaaaagcat ataccatatt caacaaaacc
     1021 ttgatggagg ctgatgctca ctacaagtca gtccggacct ggaatgagat catcccctca
     1081 aaagggtgtt tgaaagttgg aggaaggtgc catcctcatg tgaacggggt gtttttcaat
     1141 ggtataatat tagggcctga cgaccatgtc ctaatcccag agatgcaatc atccctcctc
     1201 cagcaacata tggagttgtt ggaatcttca gttatccccc tgatgcaccc cctggcagac
     1261 ccttctacag ttttcaaaga aggtgatgag gctgaggatt ttgttgaagt tcacctcccc
     1321 gatgtgtaca aacagatctc aggggttgac ctgggtctcc cgaactgggg aaagtatgta
     1381 ttgatgactg caggggccat gattggcctg gtgttgatat tttccctaat gacatggtgc
     1441 cgagttggta tccatctttg cattaaatta aagcacacca agaaaagaca gatttataca
     1501 gacatagaga tgaaccgact tggaaagtaa atcgatgcaa gggcgaattc accccaccag
     1561 tgcaggctgc ctatcagaaa gtggtggctg gtgtggctaa tgccctggcc cacaagtatc
     1621 actaagctcg ctttcttgct gtccaatttc tattaaaggt tcctttgttc cctaagtcca
     1681 actactaaac tgggggatat tatgaagggc cttgagcatc tggattctgc ctaataaaaa
     1741 acatttattt tcattgcaat gatgtattta aattatttct gaatatttta ctaaaaaggg
     1801 aatgtgggag gtcagtgcat ttaaaacata aagaaatgaa gagctagttc aaaccttggg
     1861 aaaatacact atatcttaaa ctccatgaaa gaaggtgagg ctgcaaacag ctaatgcaca
     1921 ttggcaacag cccctgatgc ctatgcctta ttcatccctc agaaaaggat tcaagtagag
     1981 gcttgatttg gaggttaaag ttttgctatg ctgtatttta cattacttat tgttttagct
     2041 gtcctcatga atgtcttttc actacccatt tgcttatcct gcatctctca gccttgactc
     2101 cactcagttc tcttgcttag agataccacc tttcccctga agtgttcctt ccatgtttta
     2161 cggcgagatg gtttctcctc gcctggccac tcagccttag ttgtctctgt tgtcttatag
     2221 aggtctactt gaagaaggaa aaacaggggg catggtttga ctgtcctgtg agcccttctt
     2281 ccctgcctcc cccactcaca gtgacccgga atccctcgac atggcagtct agcactagtg
     2341 cggccgcaga tctgcttcct cgctcactga ctcgctgcgc tcggtcgttc ggctgcggcg
     2401 agcggtatca gctcactcaa aggcggtaat acggttatcc acagaatcag gggataacgc
     2461 aggaaagaac atgtgagcaa aaggccagca aaaggccagg aaccgtaaaa aggccgcgtt
     2521 gctggcgttt ttccataggc tccgcccccc tgacgagcat cacaaaaatc gacgctcaag
     2581 tcagaggtgg cgaaacccga caggactata aagataccag gcgtttcccc ctggaagctc
     2641 cctcgtgcgc tctcctgttc cgaccctgcc gcttaccgga tacctgtccg cctttctccc
     2701 ttcgggaagc gtggcgcttt ctcatagctc acgctgtagg tatctcagtt cggtgtaggt
     2761 cgttcgctcc aagctgggct gtgtgcacga accccccgtt cagcccgacc gctgcgcctt
     2821 atccggtaac tatcgtcttg agtccaaccc ggtaagacac gacttatcgc cactggcagc
     2881 agccactggt aacaggatta gcagagcgag gtatgtaggc ggtgctacag agttcttgaa
     2941 gtggtggcct aactacggct acactagaag aacagtattt ggtatctgcg ctctgctgaa
     3001 gccagttacc ttcggaaaaa gagttggtag ctcttgatcc ggcaaacaaa ccaccgctgg
     3061 tagcggtggt ttttttgttt gcaagcagca gattacgcgc agaaaaaaag gatctcaaga
     3121 agatcctttg atcttttcta cggggtctga cgctcagtgg aacgaaaact cacgttaagg
     3181 gattttggtc atgagattat caaaaaggat cttcacctag atccttttaa attaaaaatg
     3241 aagttttaaa tcaatctaaa gtatatatga gtaaacttgg tctgacagtt accaatgctt
     3301 aatcagtgag gcacctatct cagcgatctg tctatttcgt tcatccatag ttgcctgact
     3361 ccccgtcgtg tagataacta cgatacggga gggcttacca tctggcccca gtgctgcaat
     3421 gataccgcga gacccacgct caccggctcc agatttatca gcaataaacc agccagccgg
     3481 aagggccgag cgcagaagtg gtcctgcaac tttatccgcc tccatccagt ctattaattg
     3541 ttgccgggaa gctagagtaa gtagttcgcc agttaatagt ttgcgcaacg ttgttgccat
     3601 tgctacaggc atcgtggtgt cacgctcgtc gtttggtatg gcttcattca gctccggttc
     3661 ccaacgatca aggcgagtta catgatcccc catgttgtgc aaaaaagcgg ttagctcctt
     3721 cggtcctccg atcgttgtca gaagtaagtt ggccgcagtg ttatcactca tggttatggc
     3781 agcactgcat aattctctta ctgtcatgcc atccgtaaga tgcttttctg tgactggtga
     3841 gtactcaacc aagtcattct gagaatagtg tatgcggcga ccgagttgct cttgcccggc
     3901 gtcaatacgg gataataccg cgccacatag cagaacttta aaagtgctca tcattggaaa
     3961 acgttcttcg gggcgaaaac tctcaaggat cttaccgctg ttgagatcca gttcgatgta
     4021 acccactcgt gcacccaact gatcttcagc atcttttact ttcaccagcg tttctgggtg
     4081 agcaaaaaca ggaaggcaaa atgccgcaaa aaagggaata agggcgacac ggaaatgttg
     4141 aatactcata ctcttccttt ttcaatatta ttgaagcatt tatcagggtt attgtctcat
     4201 gagcggatac atatttgaat gtatttagaa aaataaacaa ataggggttc cgcgcacatt
     4261 tccccgaaaa gtgccacctg acgtggatcc cctgaggggg cccccatggg ctagaggatc
     4321 cggcctcggc ctctgcataa ataaaaaaaa ttagtcagcc atgagcttgg cccattgcat
     4381 acgttgtatc catatcataa tatgtacatt tatattggct catgtccaac attaccgcca
     4441 tgttgacatt gattattgac tagttattaa tagtaatcaa ttacggggtc attagttcat
     4501 agcccatata tggagttccg cgttacataa cttacggtaa atggcccgcc tggctgaccg
     4561 cccaacgacc cccgcccatt gacgtcaata atgacgtatg ttcccatagt aacgccaata
     4621 gggactttcc attgacgtca atgggtggag tatttacggt aaactgccca cttggcagta
     4681 catcaagtgt atcatatgcc aagtacgccc cctattgacg tcaatgacgg taaatggccc
     4741 gcctggcatt atgcccagta catgacctta tgggactttc ctacttggca gtacatctac
     4801 gtattagtca tcgctattac catggtgatg cggttttggc agtacatcaa tgggcgtgga
     4861 tagcggtttg actcacgggg atttccaagt ctccacccca ttgacgtcaa tgggagtttg
     4921 ttttggcacc aaaatcaacg ggactttcca aaatgtcgta acaactccgc cccattgacg
     4981 caaatgggcg gtaggcgtgt acggtgggag gtctatataa gcagagctcg tttagtgaac
     5041 cgtcagatcg cctggagacg ccatccacgc tgttttgacc tccatagaag acaccgggac
     5101 cgatccagcc tcccctcgaa gcttacatgt ggtaccgagc tcggatcctg agaacttcag
     5161 ggtgagtcta tgggaccctt gatgttttct ttccccttct tttctatggt taagttcatg
     5221 tcataggaag gggagaagta acagggtaca catattgacc aaatcagggt aattttgcat
     5281 ttgtaatttt aaaaaatgct ttcttctttt aatatacttt tttgtttatc ttatttctaa
     5341 tactttccct aatctctttc tttcagggca ataatgatac aatgtatcat gcctctttgc
     5401 accattctaa agaataacag tgataatttc tgggttaagg caatagcaat atttctgcat
     5461 ataaatattt ctgcatataa attgtaactg atgtaagagg tttcatattg ctaatagcag
     5521 ctacaatcca gctaccattc tgcttttatt ttatggttgg gataaggctg gattattctg
     5581 agtccaagct aggccctttt gctaatcatg ttcatacctc ttatcttcct cccacagctc
     5641 ctgggcaacg tgctggtctg tgtgctggcc catcactttg gcaaagcacg tgagatctga
     5701 attcgccctt atccgcgg
//
