LOCUS       sequence-176705-       11518 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Plant expression vector (2x35S) for direct cloning of artificial 
            microRNAs into Arabidopsis thaliana MIR390a precursor.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11518)
  AUTHORS   Carbonell A, Takeda A, Fahlgren N, Johnson SC, Cuperus JT, 
            Carrington JC
  TITLE     New Generation of Artificial MicroRNA and Synthetic Trans-Acting 
            Small Interfering RNA Vectors for Efficient Gene Silencing in 
            Arabidopsis.
  JOURNAL   Plant Physiol. 2014 Mar 19.
  PUBMED    24647477
REFERENCE   2  (bases 1 to 11518)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Plant 
            Physiol. 2014 Mar 19."
COMMENT     Sequence Label: pMDC123SB-AtMIR390a-B/c
FEATURES             Location/Qualifiers
     source          1..11518
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             612..1163
                     /codon_start=1
                     /gene="Streptomyces hygroscopicus bar"
                     /product="phosphinothricin acetyltransferase"
                     /label=BlpR
                     /note="confers resistance to bialophos or phosphinothricin"
                     /translation="MSPERRPADIRRATEADMPAVCTIVNHYIETSTVNFRTEPQEPQE
                     WTDDLVRLRERYPWLVAEVDGEVAGIAYAGPWKARNAYDWTAESTVYVSPRHQRTGLGS
                     TLYTHLLKSLEAQGFKSVVAVIGLPNDPSVRMHEALGYAPRGMLRAAGFKHGNWHDVGF
                     WQLDFSLPVPPRPVLPVTEI"
     polyA_signal    1170..1344
                     /label=CaMV poly(A) signal
                     /note="cauliflower mosaic virus polyadenylation signal"
     misc_feature    1422..1446
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     CDS             1871..2665
                     /codon_start=1
                     /gene="aphA-3"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin"
                     /translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLKM
                     TDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEYED
                     EQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWEEDT
                     PFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKWYDIA
                     FCVRSIREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF"
     rep_origin      2752..3340
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     3241..3260
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     3494..3511
                     /label=L4440
                     /note="L4440 vector, forward primer"
     misc_feature    3526..3666
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     complement(3592..3611)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     3752..3774
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     rep_origin      4010..4204
                     /label=pVS1 oriV
                     /note="origin of replication for the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
     CDS             complement(4270..5343)
                     /codon_start=1
                     /product="replication protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 RepA
                     /translation="MSGRKPSGPVQIGAALGDDLVEKLKAAQAAQRQRIEAEARPGESW
                     QAAADRIRKESRQPPAAGAPSIRKPPKGDEQPDFFVPMLYDVGTRDSRSIMDVAVFRLS
                     KRDRRAGEVIRYELPDGHVEVSAGPAGMASVWDYDLVLMAVSHLTESMNRYREGKGDKP
                     GRVFRPHVADVLKFCRRADGGKQKDDLVETCIRLNTTHVAMQRTKKAKNGRLVTVSEGE
                     ALISRYKIVKSETGRPEYIEIELADWMYREITEGKNPDVLTVHPDYFLIDPGIGRFLYR
                     LARRAAGKAEARWLFKTIYERSGSAGEFKKFCFTVRKLIGSNDLPEYDLKEEAGQAGPI
                     LVMRYRNLIEGEASAGS"
     CDS             complement(5772..6401)
                     /codon_start=1
                     /product="stability protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 StaA
                     /translation="MKVIAVLNQKGGSGKTTIATHLARALQLAGADVLLVDSDPQGSAR
                     DWAAVREDQPLTVVGIDRPTIDRDVKAIGRRDFVVIDGAPQAADLAVSAIKAADFVLIP
                     VQPSPYDIWATADLVELVKQRIEVTDGRLQAAFVVSRAIKGTRIGGEVAEALAGYELPI
                     LESRITQRVSYPGTAAAGTTVLESEPEGDAAREVQALAAEIKSKLI"
     misc_feature    7702..7726
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     primer_bind     7914..7936
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     7928..7945
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     7929..7945
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        8371..8715
                     /label=CaMV 35S promoter
                     /note="strong constitutive promoter from cauliflower mosaic
                     virus"
     protein_bind    8775..8799
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     promoter        join(9093..9098,9099..9116,9117..9123)
                     /label=lac UV5 promoter
                     /note="E. coli lac promoter with an ""up"" mutation"
     CDS             9177..9836
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     complement(9243..9262)
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     CDS             10178..10483
                     /codon_start=1
                     /gene="ccdB"
                     /product="CcdB, a bacterial toxin that poisons DNA gyrase"
                     /label=ccdB
                     /note="Plasmids containing the ccdB gene cannot be 
                     propagated in standard E. coli strains."
                     /translation="MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDK
                     VSRELYPVVHIGDESWRMMTTDMASVPVSVIGEEVADLSHRENDIKNAINLMFWGI"
     primer_bind     10362..10381
                     /label=ccdB-fwd
                     /note="ccdB gene, forward primer"
     protein_bind    complement(10747..10771)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     terminator      10835..11087
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     primer_bind     complement(11104..11120)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(11104..11120)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(11117..11139)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    11128..11144
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(11152..11158,11159..11176,11177..11182))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    11197..11218
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(11409..11518,1..567)
                     /label=CaMV 35S promoter (enhanced)
                     /note="cauliflower mosaic virus 35S promoter with a 
                     duplicated enhancer region"
ORIGIN
        1 gccatcattg cgataaagga aaggctatcg ttcaagatgc ctctgccgac agtggtccca
       61 aagatggacc cccacccacg aggagcatcg tggaaaaaga agacgttcca accacgtctt
      121 caaagcaagt ggattgatgt gaacatggtg gagcacgaca ctctcgtcta ctccaagaat
      181 atcaaagata cagtctcaga agaccaaagg gctattgaga cttttcaaca aagggtaata
      241 tcgggaaacc tcctcggatt ccattgccca gctatctgtc acttcatcaa aaggacagta
      301 gaaaaggaag gtggcaccta caaatgccat cattgcgata aaggaaaggc tatcgttcaa
      361 gatgcctctg ccgacagtgg tcccaaagat ggacccccac ccacgaggag catcgtggaa
      421 aaagaagacg ttccaaccac gtcttcaaag caagtggatt gatgtgatat ctccactgac
      481 gtaagggatg acgcacaatc ccactatcct tcgcaagacc cttcctctat ataaggaagt
      541 tcatttcatt tggagaggac acgctgaaat caccagtctc tctctacaaa tctatctctc
      601 tcgagtctac catgagccca gaacgacgcc cggccgacat ccgccgtgcc accgaggcgg
      661 acatgccggc ggtctgcacc atcgtcaacc actacatcga gacaagcacg gtcaacttcc
      721 gtaccgagcc gcaggaaccg caggagtgga cggacgacct cgtccgtctg cgggagcgct
      781 atccctggct cgtcgccgag gtggacggcg aggtcgccgg catcgcctac gcgggcccct
      841 ggaaggcacg caacgcctac gactggacgg ccgagtcgac cgtgtacgtc tccccccgcc
      901 accagcggac gggactgggc tccacgctct acacccacct gctgaagtcc ctggaggcac
      961 agggcttcaa gagcgtggtc gctgtcatcg ggctgcccaa cgacccgagc gtgcgcatgc
     1021 acgaggcgct cggatatgcc ccccgcggca tgctgcgggc ggccggcttc aagcacggga
     1081 actggcatga cgtgggtttc tggcagctgg acttcagcct gccggtaccg ccccgtccgg
     1141 tcctgcccgt caccgagatt tgactcgagt ttctccataa taatgtgtga gtagttccca
     1201 gataagggaa ttagggttcc tatagggttt cgctcatgtg ttgagcatat aagaaaccct
     1261 tagtatgtat ttgtatttgt aaaatacttc tatcaataaa atttctaatt cctaaaacca
     1321 aaatccagta ctaaaatcca gatcccccga attaattcgg cgttaattca gtacattaaa
     1381 aacgtccgca atgtgttatt aagttgtcta agcgtcaatt tgtttacacc acaatatatc
     1441 ctgccaccag ccagccaaca gctccccgac cggcagctcg gcacaaaatc accactcgat
     1501 acaggcagcc catcagtccg ggacggcgtc agcgggagag ccgttgtaag gcggcagact
     1561 ttgctcatgt taccgatgct attcggaaga acggcaacta agctgccggg tttgaaacac
     1621 ggatgatctc gcggagggta gcatgttgat tgtaacgatg acagagcgtt gctgcctgtg
     1681 atcaccgcgg tttcaaaatc ggctccgtcg atactatgtt atacgccaac tttgaaaaca
     1741 actttgaaaa agctgttttc tggtatttaa ggttttagaa tgcaaggaac agtgaattgg
     1801 agttcgtctt gttataatta gcttcttggg gtatctttaa atactgtaga aaagaggaag
     1861 gaaataataa atggctaaaa tgagaatatc accggaattg aaaaaactga tcgaaaaata
     1921 ccgctgcgta aaagatacgg aaggaatgtc tcctgctaag gtatataagc tggtgggaga
     1981 aaatgaaaac ctatatttaa aaatgacgga cagccggtat aaagggacca cctatgatgt
     2041 ggaacgggaa aaggacatga tgctatggct ggaaggaaag ctgcctgttc caaaggtcct
     2101 gcactttgaa cggcatgatg gctggagcaa tctgctcatg agtgaggccg atggcgtcct
     2161 ttgctcggaa gagtatgaag atgaacaaag ccctgaaaag attatcgagc tgtatgcgga
     2221 gtgcatcagg ctctttcact ccatcgacat atcggattgt ccctatacga atagcttaga
     2281 cagccgctta gccgaattgg attacttact gaataacgat ctggccgatg tggattgcga
     2341 aaactgggaa gaagacactc catttaaaga tccgcgcgag ctgtatgatt ttttaaagac
     2401 ggaaaagccc gaagaggaac ttgtcttttc ccacggcgac ctgggagaca gcaacatctt
     2461 tgtgaaagat ggcaaagtaa gtggctttat tgatcttggg agaagcggca gggcggacaa
     2521 gtggtatgac attgccttct gcgtccggtc gatcagggag gatatcgggg aagaacagta
     2581 tgtcgagcta ttttttgact tactggggat caagcctgat tgggagaaaa taaaatatta
     2641 tattttactg gatgaattgt tttagtacct agaatgcatg accaaaatcc cttaacgtga
     2701 gttttcgttc cactgagcgt cagaccccgt agaaaagatc aaaggatctt cttgagatcc
     2761 tttttttctg cgcgtaatct gctgcttgca aacaaaaaaa ccaccgctac cagcggtggt
     2821 ttgtttgccg gatcaagagc taccaactct ttttccgaag gtaactggct tcagcagagc
     2881 gcagatacca aatactgtcc ttctagtgta gccgtagtta ggccaccact tcaagaactc
     2941 tgtagcaccg cctacatacc tcgctctgct aatcctgtta ccagtggctg ctgccagtgg
     3001 cgataagtcg tgtcttaccg ggttggactc aagacgatag ttaccggata aggcgcagcg
     3061 gtcgggctga acggggggtt cgtgcacaca gcccagcttg gagcgaacga cctacaccga
     3121 actgagatac ctacagcgtg agctatgaga aagcgccacg cttcccgaag ggagaaaggc
     3181 ggacaggtat ccggtaagcg gcagggtcgg aacaggagag cgcacgaggg agcttccagg
     3241 gggaaacgcc tggtatcttt atagtcctgt cgggtttcgc cacctctgac ttgagcgtcg
     3301 atttttgtga tgctcgtcag gggggcggag cctatggaaa aacgccagca acgcggcctt
     3361 tttacggttc ctggcctttt gctggccttt tgctcacatg ttctttcctg cgttatcccc
     3421 tgattctgtg gataaccgta ttaccgcctt tgagtgagct gataccgctc gccgcagccg
     3481 aacgaccgag cgcagcgagt cagtgagcga ggaagcggaa gagcgcctga tgcggtattt
     3541 tctccttacg catctgtgcg gtatttcaca ccgcatatgg tgcactctca gtacaatctg
     3601 ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg
     3661 gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg
     3721 gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca
     3781 ccgtcatcac cgaaacgcgc gaggcagggt gccttgatgt gggcgccggc ggtcgagtgg
     3841 cgacggcgcg gcttgtccgc gccctggtag attgcctggc cgtaggccag ccatttttga
     3901 gcggccagcg gccgcgatag gccgacgcga agcggcgggg cgtagggagc gcagcgaccg
     3961 aagggtaggc gctttttgca gctcttcggc tgtgcgctgg ccagacagtt atgcacaggc
     4021 caggcgggtt ttaagagttt taataagttt taaagagttt taggcggaaa aatcgccttt
     4081 tttctctttt atatcagtca cttacatgtg tgaccggttc ccaatgtacg gctttgggtt
     4141 cccaatgtac gggttccggt tcccaatgta cggctttggg ttcccaatgt acgtgctatc
     4201 cacaggaaag agaacttttc gacctttttc ccctgctagg gcaatttgcc ctagcatctg
     4261 ctccgtacat taggaaccgg cggatgcttc gccctcgatc aggttgcggt agcgcatgac
     4321 taggatcggg ccagcctgcc ccgcctcctc cttcaaatcg tactccggca ggtcatttga
     4381 cccgatcagc ttgcgcacgg tgaaacagaa cttcttgaac tctccggcgc tgccactgcg
     4441 ttcgtagatc gtcttgaaca accatctggc ttctgccttg cctgcggcgc ggcgtgccag
     4501 gcggtagaga aaacggccga tgccgggatc gatcaaaaag taatcggggt gaaccgtcag
     4561 cacgtccggg ttcttgcctt ctgtgatctc gcggtacatc caatcagcta gctcgatctc
     4621 gatgtactcc ggccgcccgg tttcgctctt tacgatcttg tagcggctaa tcaaggcttc
     4681 accctcggat accgtcacca ggcggccgtt cttggccttc ttcgtacgct gcatggcaac
     4741 gtgcgtggtg tttaaccgaa tgcaggtttc taccaggtcg tctttctgct ttccgccatc
     4801 ggctcgccgg cagaacttga gtacgtccgc aacgtgtgga cggaacacgc ggccgggctt
     4861 gtctcccttc ccttcccggt atcggttcat ggattcggtt agatgggaaa ccgccatcag
     4921 taccaggtcg taatcccaca cactggccat gccggccggc cctgcggaaa cctctacgtg
     4981 cccgtctgga agctcgtagc ggatcacctc gccagctcgt cggtcacgct tcgacagacg
     5041 gaaaacggcc acgtccatga tgctgcgact atcgcgggtg cccacgtcat agagcatcgg
     5101 aacgaaaaaa tctggttgct cgtcgccctt gggcggcttc ctaatcgacg gcgcaccggc
     5161 tgccggcggt tgccgggatt ctttgcggat tcgatcagcg gccgcttgcc acgattcacc
     5221 ggggcgtgct tctgcctcga tgcgttgccg ctgggcggcc tgcgcggcct tcaacttctc
     5281 caccaggtca tcacccagcg ccgcgccgat ttgtaccggg ccggatggtt tgcgaccgct
     5341 cacgccgatt cctcgggctt gggggttcca gtgccattgc agggccggca ggcaacccag
     5401 ccgcttacgc ctggccaacc gcccgttcct ccacacatgg ggcattccac ggcgtcggtg
     5461 cctggttgtt cttgattttc catgccgcct cctttagccg ctaaaattca tctactcatt
     5521 tattcatttg ctcatttact ctggtagctg cgcgatgtat tcagatagca gctcggtaat
     5581 ggtcttgcct tggcgtaccg cgtacatctt cagcttggtg tgatcctccg ccggcaactg
     5641 aaagttgacc cgcttcatgg ctggcgtgtc tgccaggctg gccaacgttg cagccttgct
     5701 gctgcgtgcg ctcggacggc cggcacttag cgtgtttgtg cttttgctca ttttctcttt
     5761 acctcattaa ctcaaatgag ttttgattta atttcagcgg ccagcgcctg gacctcgcgg
     5821 gcagcgtcgc cctcgggttc tgattcaaga acggttgtgc cggcggcggc agtgcctggg
     5881 tagctcacgc gctgcgtgat acgggactca agaatgggca gctcgtaccc ggccagcgcc
     5941 tcggcaacct caccgccgat gcgcgtgcct ttgatcgccc gcgacacgac aaaggccgct
     6001 tgtagccttc catccgtgac ctcaatgcgc tgcttaacca gctccaccag gtcggcggtg
     6061 gcccatatgt cgtaagggct tggctgcacc ggaatcagca cgaagtcggc tgccttgatc
     6121 gcggacacag ccaagtccgc cgcctggggc gctccgtcga tcactacgaa gtcgcgccgg
     6181 ccgatggcct tcacgtcgcg gtcaatcgtc gggcggtcga tgccgacaac ggttagcggt
     6241 tgatcttccc gcacggccgc ccaatcgcgg gcactgccct ggggatcgga atcgactaac
     6301 agaacatcgg ccccggcgag ttgcagggcg cgggctagat gggttgcgat ggtcgtcttg
     6361 cctgacccgc ctttctggtt aagtacagcg ataaccttca tgcgttcccc ttgcgtattt
     6421 gtttatttac tcatcgcatc atatacgcag cgaccgcatg acgcaagctg ttttactcaa
     6481 atacacatca cctttttaga cggcggcgct cggtttcttc agcggccaag ctggccggcc
     6541 aggccgccag cttggcatca gacaaaccgg ccaggatttc atgcagccgc acggttgaga
     6601 cgtgcgcggg cggctcgaac acgtacccgg ccgcgatcat ctccgcctcg atctcttcgg
     6661 taatgaaaaa cggttcgtcc tggccgtcct ggtgcggttt catgcttgtt cctcttggcg
     6721 ttcattctcg gcggccgcca gggcgtcggc ctcggtcaat gcgtcctcac ggaaggcacc
     6781 gcgccgcctg gcctcggtgg gcgtcacttc ctcgctgcgc tcaagtgcgc ggtacagggt
     6841 cgagcgatgc acgccaagca gtgcagccgc ctctttcacg gtgcggcctt cctggtcgat
     6901 cagctcgcgg gcgtgcgcga tctgtgccgg ggtgagggta gggcgggggc caaacttcac
     6961 gcctcgggcc ttggcggcct cgcgcccgct ccgggtgcgg tcgatgatta gggaacgctc
     7021 gaactcggca atgccggcga acacggtcaa caccatgcgg ccggccggcg tggtggtgtc
     7081 ggcccacggc tctgccaggc tacgcaggcc cgcgccggcc tcctggatgc gctcggcaat
     7141 gtccagtagg tcgcgggtgc tgcgggccag gcggtctagc ctggtcactg tcacaacgtc
     7201 gccagggcgt aggtggtcaa gcatcctggc cagctccggg cggtcgcgcc tggtgccggt
     7261 gatcttctcg gaaaacagct tggtgcagcc ggccgcgtgc agttcggccc gttggttggt
     7321 caagtcctgg tcgtcggtgc tgacgcgggc atagcccagc aggccagcgg cggcgctctt
     7381 gttcatggcg taatgtctcc ggttctagtc gcaagtattc tactttatgc gactaaaaca
     7441 cgcgacaaga aaacgccagg aaaagggcag ggcggcagcc tgtcgcgtaa cttaggactt
     7501 gtgcgacatg tcgttttcag aagacggctg cactgaacgt cagaagccga ctgcactata
     7561 gcagcggagg ggttggatca aagtactttg atcccgaggg gaaccctgtg gttggcatgc
     7621 acatacaaat ggacgaacgg ataaaccttt tcacgccctt ttaaatatcc gattattcta
     7681 ataaacgctc ttttctctta ggtttacccg ccaatatatc ctgtcaaaca ctgatagttt
     7741 aaactgaagg cgggaaacga caatctgatc caagctcaag ctgctctagc attcgccatt
     7801 caggctgcgc aactgttggg aagggcgatc ggtgcgggcc tcttcgctat tacgccagct
     7861 ggcgaaaggg ggatgtgctg caaggcgatt aagttgggta acgccagggt tttcccagtc
     7921 acgacgttgt aaaacgacgg ccagtgccaa gcttgcatgc ctgcaggtca acatggtgga
     7981 gcacgacaca cttgtctact ccaaaaatat caaagataca gtctcagaag accaaagggc
     8041 aattgagact tttcaacaaa gggtaatatc cggaaacctc ctcggattcc attgcccagc
     8101 tatctgtcac tttattgtga agatagtgga aaaggaaggt ggctcctaca aatgccatca
     8161 ttgcgataaa ggaaaggcca tcgttgaaga tgcctctgcc gacagtggtc ccaaagatgg
     8221 acccccaccc acgaggagca tcgtggaaaa agaagacgtt ccaaccacgt cttcaaagca
     8281 agtggattga tgtgataaca tggtggagca cgacacactt gtctactcca aaaatatcaa
     8341 agatacagtc tcagaagacc aaagggcaat tgagactttt caacaaaggg taatatccgg
     8401 aaacctcctc ggattccatt gcccagctat ctgtcacttt attgtgaaga tagtggaaaa
     8461 ggaaggtggc tcctacaaat gccatcattg cgataaagga aaggccatcg ttgaagatgc
     8521 ctctgccgac agtggtccca aagatggacc cccacccacg aggagcatcg tggaaaaaga
     8581 agacgttcca accacgtctt caaagcaagt ggattgatgt gatatctcca ctgacgtaag
     8641 ggatgacgca caatcccact atccttcgca agacccttcc tctatataag gaagttcatt
     8701 tcatttggag aggacctcga ctctagagga tccccgggta ccgggccccc cctcgaggcg
     8761 cgccaagcta tcaaacaagt ttgtacaaaa aagcaggctc cgcggccgcc cccttcacct
     8821 atagggggga aaaaaaggta gtcatcagat atatattttg gtaagaaaat atagaaatga
     8881 ataatttcac gtttaacgaa gaggagatga cgtgtgttcc ttcgaacccg agttttgttc
     8941 gtctataaat agcaccttct cttctccttc ttcctcactt ccatcttttt agcttcacta
     9001 tctctctata atcggtttta tctttctcta agtcacaacc caaaaaaaca aagtagagaa
     9061 gaatctgtaa gagaccatta ggcaccccag gctttacact ttatgcttcc ggctcgtata
     9121 atgtgtggat tttgagttag gagccgtcga gattttcagg agctaaggaa gctaaaatgg
     9181 agaaaaaaat cactggatat accaccgttg atatatccca atggcatcgt aaagaacatt
     9241 ttgaggcatt tcagtcagtt gctcaatgta cctataacca gaccgttcag ctggatatta
     9301 cggccttttt aaagaccgta aagaaaaata agcacaagtt ttatccggcc tttattcaca
     9361 ttcttgcccg cctgatgaat gctcatccgg agttccgtat ggcaatgaaa gacggtgagc
     9421 tggtgatatg ggatagtgtt cacccttgtt acaccgtttt ccatgagcaa actgaaacgt
     9481 tttcatcgct ctggagtgaa taccacgacg atttccggca gtttctacac atatattcgc
     9541 aagatgtggc gtgttacggt gaaaacctgg cctatttccc taaagggttt attgagaata
     9601 tgtttttcgt ctcagccaat ccctgggtga gtttcaccag ttttgattta aacgtggcca
     9661 atatggacaa cttcttcgcc cccgttttca ccatgggcaa atattatacg caaggcgaca
     9721 aggtgctgat gccgctggcg attcaggttc atcatgccgt ttgtgatggc ttccatgtcg
     9781 gcagaatgct taatgaatta caacagtact gcgatgagtg gcagggcggg gcgtaaacgc
     9841 gtggagccgg cttactaaaa gccagataac agtatgcgta tttgcgcgct gatttttgcg
     9901 gtataagaat atatactgat atgtataccc gaagtatgtc aaaaagaggt atgctatgaa
     9961 gcagcgtatt acagtgacag ttgacagcga cagctatcag ttgctcaagg catatatgat
    10021 gtcaatatct ccggtctggt aagcacaacc atgcagaatg aagcccgtcg tctgcgtgcc
    10081 gaacgctgga aagcggaaaa tcaggaaggg atggctgagg tcgcccggtt tattgaaatg
    10141 aacggctctt ttgctgacga gaacaggggc tggtgaaatg cagtttaagg tttacaccta
    10201 taaaagagag agccgttatc gtctgtttgt ggatgtacag agtgatatta ttgacacgcc
    10261 cggccgacgg atggtgatcc ccctggccag tgcacgtctg ctgtcagata aagtctcccg
    10321 tgaactttac ccggtggtgc atatcgggga tgaaagctgg cgcatgatga ccaccgatat
    10381 ggccagtgtg ccggtttccg ttatcgggga agaagtggct gatctcagcc accgcgaaaa
    10441 tgacatcaaa aacgccatta acctgatgtt ctggggaata taaatgtcag gctcccttat
    10501 acacagccag tctgcacctc gacggtctca cattggctct tcttactaca atgaaaaagg
    10561 ccgaggcaaa acgcctaaaa tcacttgaga atcaattctt tttactgtcc atttaagcta
    10621 tcttttataa acgtgtctta ttttctatct cttttgttta aactaagaaa ctatagtatt
    10681 ttgtctaaaa caaaacatga aagaacagat tagatctcat ctttagtctc aagggtgggc
    10741 gcgccgaccc agctttcttg tacaaagtgg ttcgataatt cttaattaac tagttctaga
    10801 gcggccgcca ccgcggtgga gctcgaattt ccccgatcgt tcaaacattt ggcaataaag
    10861 tttcttaaga ttgaatcctg ttgccggtct tgcgatgatt atcatataat ttctgttgaa
    10921 ttacgttaag catgtaataa ttaacatgta atgcatgacg ttatttatga gatgggtttt
    10981 tatgattaga gtcccgcaat tatacattta atacgcgata gaaaacaaaa tatagcgcgc
    11041 aaactaggat aaattatcgc gcgcggtgtc atctatgtta ctagatcggg aattcgtaat
    11101 catgtcatag ctgtttcctg tgtgaaattg ttatccgctc acaattccac acaacatacg
    11161 agccggaagc ataaagtgta aagcctgggg tgcctaatga gtgagctaac tcacattaat
    11221 tgcgttgcgc tcactgcccg ctttccagtc gggaaacctg tcgtgccagc tgcattaatg
    11281 aatcggccaa cgcgcgggga gaggcggttt gcgtattggc tagagcagct tgccaacatg
    11341 gtggagcacg acactctcgt ctactccaag aatatcaaag atacagtctc agaagaccaa
    11401 agggctattg agacttttca acaaagggta atatcgggaa acctcctcgg attccattgc
    11461 ccagctatct gtcacttcat caaaaggaca gtagaaaagg aaggtggcac ctacaaat
//
