LOCUS       sequence-176710-        9839 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Lentiviral over-expression vector for YAP.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9839)
  AUTHORS   Qin H, Hejna M, Liu Y, Percharde M, Wossidlo M, Blouin L, 
            Durruthy-Durruthy J, Wong P, Qi Z, Yu J, Qi LS, Sebastiano V, Song 
            JS, Ramalho-Santos M
  TITLE     YAP Induces Human Naive Pluripotency.
  JOURNAL   Cell Rep. 2016 Mar 15;14(10):2301-12. doi: 
            10.1016/j.celrep.2016.02.036. Epub 2016 Mar 3.
  PUBMED    26947063
REFERENCE   2  (bases 1 to 9839)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.celrep.2016.02.036"; journalName: "Cell Rep"; date: 
            "2016-03-15- 15"; volume: "14"; issue: "10"; pages: "2301-12"
COMMENT     Sequence Label: pGAMA-YAP
FEATURES             Location/Qualifiers
     source          1..9839
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    complement(1529..1553)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             1579..1620
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     CDS             1624..1677
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     regulatory      1684..1693
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             1690..2400
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     primer_bind     complement(1835..1853)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     2103..2122
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     protein_bind    complement(2420..2453)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     misc_feature    2509..3097
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(2562..2582)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(2980..2991)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(3100..3116)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(3101..3117)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     LTR             3626..3806
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     rep_origin      complement(3868..4456)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(3948..3967)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(4627..5418,5419..5487))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     5250..5269
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(5488..5592)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(5667..5686)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        5858..6237
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        6239..6437
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     6392..6412
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     LTR             6455..6635
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    6682..6807
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    7304..7537
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             7722..7766
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    8064..8181
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     protein_bind    complement(8434..8467)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     primer_bind     8471..8491
                     /label=mU6-F
                     /note="Mouse U6 promoter, forward primer"
     promoter        8629..8840
                     /label=EF-1-alpha core promoter
                     /note="core promoter for human elongation factor 
                     EF-1-alpha"
     intron          8841..9779
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     9736..9756
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     protein_bind    9795..9819
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
ORIGIN
        1 atggatcccg ggcagcagcc gccgcctcaa ccggcccccc agggccaagg gcagccgcct
       61 tcgcagcccc cgcaggggca gggcccgccg tccggacccg ggcaaccggc acccgcggcg
      121 acccaggcgg cgccgcaggc accccccgcc gggcatcaga tcgtgcacgt ccgcggggac
      181 tcggagaccg acctggaggc gctcttcaac gccgtcatga accccaagac ggccaacgtg
      241 ccccagaccg tgcccatgag gctccggaag ctgcccgact ccttcttcaa gccgccggag
      301 cccaaatccc actcccgaca ggccagtact gatgcaggca ctgcaggagc cctgactcca
      361 cagcatgttc gagctcattc ctctccagct tctctgcagt tgggagctgt ttctcctggg
      421 acactgaccc ccactggagt agtctctggc ccagcagcta cacccacagc tcagcatctt
      481 cgacagtctt cttttgagat acctgatgat gtacctctgc cagcaggttg ggagatggca
      541 aagacatctt ctggtcagag atacttctta aatcacatcg atcagacaac aacatggcag
      601 gaccccagga aggccatgct gtcccagatg aacgtcacag cccccaccag tccaccagtg
      661 cagcagaata tgatgaactc ggcttcaggt cctcttcctg atggatggga acaagccatg
      721 actcaggatg gagaaattta ctatataaac cataagaaca agaccacctc ttggctagac
      781 ccaaggcttg accctcgttt tgccatgaac cagagaatca gtcagagtgc tccagtgaaa
      841 cagccaccac ccctggctcc ccagagccca cagggaggcg tcatgggtgg cagcaactcc
      901 aaccagcagc aacagatgcg actgcagcaa ctgcagatgg agaaggagag gctgcggctg
      961 aaacagcaag aactgcttcg gcaggcaatg cggaatatca atcccagcac agcaaattct
     1021 ccaaaatgtc aggagttagc cctgcgtagc cagttaccaa cactggagca ggatggtggg
     1081 actcaaaatc cagtgtcttc tcccgggatg tctcaggaat tgagaacaat gacgaccaat
     1141 agctcagatc ctttccttaa cagtggcacc tatcactctc gagatgagag tacagacagt
     1201 ggactaagca tgagcagcta cagtgtccct cgaaccccag atgacttcct gaacagtgtg
     1261 gatgagatgg atacaggtga tactatcaac caaagcaccc tgccctcaca gcagaaccgt
     1321 ttcccagact accttgaagc cattcctggg acaaatgtgg accttggaac actggaagga
     1381 gatggaatga acatagaagg agaggagctg atgccaagtc tgcaggaagc tttgagttct
     1441 gacatcctta atgacatgga gtctgttttg gctgccacca agctagataa agaaagcttt
     1501 cttacatggt taaagggtgg gcgcgccgac ccagctttct tgtacaaagt ggttgatcta
     1561 gagggcccgc ggttcgaagg taagcctatc cctaaccctc tcctcggtct cgattctacg
     1621 cgtgagggca gaggaagtct gctaacatgc ggtgacgtcg aggagaatcc tggcccaccg
     1681 gtcgccacca tggtgagcaa gggcgaggag gataacatgg ccatcatcaa ggagttcatg
     1741 cgcttcaagg tgcacatgga gggctccgtg aacggccacg agttcgagat cgagggcgag
     1801 ggcgagggcc gcccctacga gggcacccag accgccaagc tgaaggtgac caagggtggc
     1861 cccctgccct tcgcctggga catcctgtcc cctcagttca tgtacggctc caaggcctac
     1921 gtgaagcacc ccgccgacat ccccgactac ttgaagctgt ccttccccga gggcttcaag
     1981 tgggagcgcg tgatgaactt cgaggacggc ggcgtggtga ccgtgaccca ggactcctcc
     2041 ctgcaggacg gcgagttcat ctacaaggtg aagctgcgcg gcaccaactt cccctccgac
     2101 ggccccgtaa tgcagaagaa gaccatgggc tgggaggcct cctccgagcg gatgtacccc
     2161 gaggacggcg ccctgaaggg cgagatcaag cagaggctga agctgaagga cggcggccac
     2221 tacgacgctg aggtcaagac cacctacaag gccaagaagc ccgtgcagct gcccggcgcc
     2281 tacaacgtca acatcaagtt ggacatcacc tcccacaacg aggactacac catcgtggaa
     2341 cagtacgaac gcgccgaggg ccgccactcc accggcggca tggacgagct gtacaagtaa
     2401 gaattcgtcg agggacctaa taacttcgta tagcatacat tatacgaagt tatacatgtt
     2461 taagggttcc ggttccacta ggtacaattc gatatcaagc ttatcgataa tcaacctctg
     2521 gattacaaaa tttgtgaaag attgactggt attcttaact atgttgctcc ttttacgcta
     2581 tgtggatacg ctgctttaat gcctttgtat catgctattg cttcccgtat ggctttcatt
     2641 ttctcctcct tgtataaatc ctggttgctg tctctttatg aggagttgtg gcccgttgtc
     2701 aggcaacgtg gcgtggtgtg cactgtgttt gctgacgcaa cccccactgg ttggggcatt
     2761 gccaccacct gtcagctcct ttccgggact ttcgctttcc ccctccctat tgccacggcg
     2821 gaactcatcg ccgcctgcct tgcccgctgc tggacagggg ctcggctgtt gggcactgac
     2881 aattccgtgg tgttgtcggg gaaatcatcg tcctttcctt ggctgctcgc ctgtgttgcc
     2941 acctggattc tgcgcgggac gtccttctgc tacgtccctt cggccctcaa tccagcggac
     3001 cttccttccc gcggcctgct gccggctctg cggcctcttc cgcgtcttcg ccttcgccct
     3061 cagacgagtc ggatctccct ttgggccgcc tccccgcatc gataccgtcg acctcgatcg
     3121 agacctagaa aaacatggag caatcacaag tagcaataca gcagctacca atgctgattg
     3181 tgcctggcta gaagcacaag aggaggagga ggtgggtttt ccagtcacac ctcaggtacc
     3241 tttaagacca atgacttaca aggcagctgt agatcttagc cactttttaa aagaaaaggg
     3301 gggactggaa gggctaattc actcccaacg aagacaagat atccttgatc tgtggatcta
     3361 ccacacacaa ggctacttcc ctgattggca gaactacaca ccagggccag ggatcagata
     3421 tccactgacc tttggatggt gctacaagct agtaccagtt gagcaagaga aggtagaaga
     3481 agccaatgaa ggagagaaca cccgcttgtt acaccctgtg agcctgcatg ggatggatga
     3541 cccggagaga gaagtattag agtggaggtt tgacagccgc ctagcatttc atcacatggc
     3601 ccgagagctg catccggact gtactgggtc tctctggtta gaccagatct gagcctggga
     3661 gctctctggc taactaggga acccactgct taagcctcaa taaagcttgc cttgagtgct
     3721 tcaagtagtg tgtgcccgtc tgttgtgtga ctctggtaac tagagatccc tcagaccctt
     3781 ttagtcagtg tggaaaatct ctagcagcat gtgagcaaaa ggccagcaaa aggccaggaa
     3841 ccgtaaaaag gccgcgttgc tggcgttttt ccataggctc cgcccccctg acgagcatca
     3901 caaaaatcga cgctcaagtc agaggtggcg aaacccgaca ggactataaa gataccaggc
     3961 gtttccccct ggaagctccc tcgtgcgctc tcctgttccg accctgccgc ttaccggata
     4021 cctgtccgcc tttctccctt cgggaagcgt ggcgctttct catagctcac gctgtaggta
     4081 tctcagttcg gtgtaggtcg ttcgctccaa gctgggctgt gtgcacgaac cccccgttca
     4141 gcccgaccgc tgcgccttat ccggtaacta tcgtcttgag tccaacccgg taagacacga
     4201 cttatcgcca ctggcagcag ccactggtaa caggattagc agagcgaggt atgtaggcgg
     4261 tgctacagag ttcttgaagt ggtggcctaa ctacggctac actagaagaa cagtatttgg
     4321 tatctgcgct ctgctgaagc cagttacctt cggaaaaaga gttggtagct cttgatccgg
     4381 caaacaaacc accgctggta gcggtggttt ttttgtttgc aagcagcaga ttacgcgcag
     4441 aaaaaaagga tctcaagaag atcctttgat cttttctacg gggtctgacg ctcagtggaa
     4501 cgaaaactca cgttaaggga ttttggtcat gagattatca aaaaggatct tcacctagat
     4561 ccttttaaat taaaaatgaa gttttaaatc aatctaaagt atatatgagt aaacttggtc
     4621 tgacagttac caatgcttaa tcagtgaggc acctatctca gcgatctgtc tatttcgttc
     4681 atccatagtt gcctgactcc ccgtcgtgta gataactacg atacgggagg gcttaccatc
     4741 tggccccagt gctgcaatga taccgcgaga cccacgctca ccggctccag atttatcagc
     4801 aataaaccag ccagccggaa gggccgagcg cagaagtggt cctgcaactt tatccgcctc
     4861 catccagtct attaattgtt gccgggaagc tagagtaagt agttcgccag ttaatagttt
     4921 gcgcaacgtt gttgccattg ctacaggcat cgtggtgtca cgctcgtcgt ttggtatggc
     4981 ttcattcagc tccggttccc aacgatcaag gcgagttaca tgatccccca tgttgtgcaa
     5041 aaaagcggtt agctccttcg gtcctccgat cgttgtcaga agtaagttgg ccgcagtgtt
     5101 atcactcatg gttatggcag cactgcataa ttctcttact gtcatgccat ccgtaagatg
     5161 cttttctgtg actggtgagt actcaaccaa gtcattctga gaatagtgta tgcggcgacc
     5221 gagttgctct tgcccggcgt caatacggga taataccgcg ccacatagca gaactttaaa
     5281 agtgctcatc attggaaaac gttcttcggg gcgaaaactc tcaaggatct taccgctgtt
     5341 gagatccagt tcgatgtaac ccactcgtgc acccaactga tcttcagcat cttttacttt
     5401 caccagcgtt tctgggtgag caaaaacagg aaggcaaaat gccgcaaaaa agggaataag
     5461 ggcgacacgg aaatgttgaa tactcatact cttccttttt caatattatt gaagcattta
     5521 tcagggttat tgtctcatga gcggatacat atttgaatgt atttagaaaa ataaacaaat
     5581 aggggttccg cgcacatttc cccgaaaagt gccacctgac gtcgacggat cgggagatct
     5641 cccgatcccc tatggtgcac tctcagtaca atctgctctg atgccgcata gttaagccag
     5701 tatctgctcc ctgcttgtgt gttggaggtc gctgagtagt gcgcgagcaa aatttaagct
     5761 acaacaaggc aaggcttgac cgacaattgc atgaagaatc tgcttagggt taggcgtttt
     5821 gcgctgcttc gcgatgtacg ggccagatat acgcgttgac attgattatt gactagttat
     5881 taatagtaat caattacggg gtcattagtt catagcccat atatggagtt ccgcgttaca
     5941 taacttacgg taaatggccc gcctggctga ccgcccaacg acccccgccc attgacgtca
     6001 ataatgacgt atgttcccat agtaacgcca atagggactt tccattgacg tcaatgggtg
     6061 gagtatttac ggtaaactgc ccacttggca gtacatcaag tgtatcatat gccaagtacg
     6121 ccccctattg acgtcaatga cggtaaatgg cccgcctggc attatgccca gtacatgacc
     6181 ttatgggact ttcctacttg gcagtacatc tacgtattag tcatcgctat taccatggtg
     6241 atgcggtttt ggcagtacat caatgggcgt ggatagcggt ttgactcacg gggatttcca
     6301 agtctccacc ccattgacgt caatgggagt ttgttttggc accaaaatca acgggacttt
     6361 ccaaaatgtc gtaacaactc cgccccattg acgcaaatgg gcggtaggcg tgtacggtgg
     6421 gaggtctata taagcagcgc gttttgcctg tactgggtct ctctggttag accagatctg
     6481 agcctgggag ctctctggct aactagggaa cccactgctt aagcctcaat aaagcttgcc
     6541 ttgagtgctt caagtagtgt gtgcccgtct gttgtgtgac tctggtaact agagatccct
     6601 cagacccttt tagtcagtgt ggaaaatctc tagcagtggc gcccgaacag ggacttgaaa
     6661 gcgaaaggga aaccagagga gctctctcga cgcaggactc ggcttgctga agcgcgcacg
     6721 gcaagaggcg aggggcggcg actggtgagt acgccaaaaa ttttgactag cggaggctag
     6781 aaggagagag atgggtgcga gagcgtcagt attaagcggg ggagaattag atcgcgatgg
     6841 gaaaaaattc ggttaaggcc agggggaaag aaaaaatata aattaaaaca tatagtatgg
     6901 gcaagcaggg agctagaacg attcgcagtt aatcctggcc tgttagaaac atcagaaggc
     6961 tgtagacaaa tactgggaca gctacaacca tcccttcaga caggatcaga agaacttaga
     7021 tcattatata atacagtagc aaccctctat tgtgtgcatc aaaggataga gataaaagac
     7081 accaaggaag ctttagacaa gatagaggaa gagcaaaaca aaagtaagac caccgcacag
     7141 caagcggccg gccgctgatc ttcagacctg gaggaggaga tatgagggac aattggagaa
     7201 gtgaattata taaatataaa gtagtaaaaa ttgaaccatt aggagtagca cccaccaagg
     7261 caaagagaag agtggtgcag agagaaaaaa gagcagtggg aataggagct ttgttccttg
     7321 ggttcttggg agcagcagga agcactatgg gcgcagcgtc aatgacgctg acggtacagg
     7381 ccagacaatt attgtctggt atagtgcagc agcagaacaa tttgctgagg gctattgagg
     7441 cgcaacagca tctgttgcaa ctcacagtct ggggcatcaa gcagctccag gcaagaatcc
     7501 tggctgtgga aagataccta aaggatcaac agctcctggg gatttggggt tgctctggaa
     7561 aactcatttg caccactgct gtgccttgga atgctagttg gagtaataaa tctctggaac
     7621 agatttggaa tcacacgacc tggatggagt gggacagaga aattaacaat tacacaagct
     7681 taatacactc cttaattgaa gaatcgcaaa accagcaaga aaagaatgaa caagaattat
     7741 tggaattaga taaatgggca agtttgtgga attggtttaa cataacaaat tggctgtggt
     7801 atataaaatt attcataatg atagtaggag gcttggtagg tttaagaata gtttttgctg
     7861 tactttctat agtgaataga gttaggcagg gatattcacc attatcgttt cagacccacc
     7921 tcccaacccc gaggggaccc gacaggcccg aaggaataga agaagaaggt ggagagagag
     7981 acagagacag atccattcga ttagtgaacg gatcggcact gcgtgcgcca attctgcaga
     8041 caaatggcag tattcatcca caattttaaa agaaaagggg ggattggggg gtacagtgca
     8101 ggggaaagaa tagtagacat aatagcaaca gacatacaaa ctaaagaatt acaaaaacaa
     8161 attacaaaaa ttcaaaattt tcgggtttat tacagggaca gcagagatcc agtttggtta
     8221 gtaccgggcc cgctctagag atccgacgcg ccatctctag gcccgcgccg gccccctcgc
     8281 acggacttgt gggagaagct cggctactcc cctgccccgg ttaatttgca tataatattt
     8341 cctagtaact atagaggctt aatgtgcgat aaaagacaga taatctgttc tttttaatac
     8401 tagctacatt ttacatgata ggcttggatt tctataactt cgtatagcat acattatacg
     8461 aagttataaa cagcacaaaa ggaaactcac cctaactgta aagtaattgt gtgttttgag
     8521 actataagta tcccttggag aaccaccttg ttggtttttc tcgagtacta ggatccatta
     8581 ggcggccgcg tggataaccg tattaccgcc atgcatgtgc ccgtcagtgg gcagagcgca
     8641 catcgcccac agtccccgag aagttggggg gaggggtcgg caattgaacc ggtgcctaga
     8701 gaaggtggcg cggggtaaac tgggaaagtg atgtcgtgta ctggctccgc ctttttcccg
     8761 agggtggggg agaaccgtat ataagtgcag tagtcgccgt gaacgttctt tttcgcaacg
     8821 ggtttgccgc cagaacacag gtaagtgccg tgtgtggttc ccgcgggcct ggcctcttta
     8881 cgggttatgg cccttgcgtg ccttgaatta cttccacctg gctgcagtac gtgattcttg
     8941 atcccgagct tcgggttgga agtgggtggg agagttcgag gccttgcgct taaggagccc
     9001 cttcgcctcg tgcttgagtt gaggcctggc ctgggcgctg gggccgccgc gtgcgaatct
     9061 ggtggcacct tcgcgcctgt ctcgctgctt tcgataagtc tctagccatt taaaattttt
     9121 gatgacctgc tgcgacgctt tttttctggc aagatagtct tgtaaatgcg ggccaagatc
     9181 tgcacactgg tatttcggtt tttggggccg cgggcggcga cggggcccgt gcgtcccagc
     9241 gcacatgttc ggcgaggcgg ggcctgcgag cgcggccacc gagaatcgga cgggggtagt
     9301 ctcaagctgg ccggcctgct ctggtgcctg gcctcgcgcc gccgtgtatc gccccgccct
     9361 gggcggcaag gctggcccgg tcggcaccag ttgcgtgagc ggaaagatgg ccgcttcccg
     9421 gccctgctgc agggagctca aaatggagga cgcggcgctc gggagagcgg gcgggtgagt
     9481 cacccacaca aaggaaaagg gcctttccgt cctcagccgt cgcttcatgt gactccacgg
     9541 agtaccgggc gccgtccagg cacctcgatt agttctcgag cttttggagt acgtcgtctt
     9601 taggttgggg ggaggggttt tatgcgatgg agtttcccca cactgagtgg gtggagactg
     9661 aagttaggcc agcttggcac ttgatgtaat tctccttgga atttgccctt tttgagtttg
     9721 gatcttggtt cattctcaag cctcagacag tggttcaaag tttttttctt ccatttcagg
     9781 tgtcgtgagc tagcacaagt ttgtacaaaa aagcaggctc cgcggccgcc cccttcacc
//
