LOCUS       sequence-176721-        3682 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Can be used for in situ hybridisation or other purposes.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3682)
  AUTHORS   Hall TE, Martel N, Lo HP, Xiong Z, Parton RG
  TITLE     A plasmid library of full-length zebrafish rab proteins for in vivo 
            cell biology.
  JOURNAL   Cell Logist. 2017 Mar 1;7(1):e1301151. doi: 
            10.1080/21592799.2017.1301151. eCollection 2017.
  PUBMED    28396820
REFERENCE   2  (bases 1 to 3682)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell 
            Logist."; date: "2017-03-1"; volume: "7(1):e1301151. doi"; pages: " 
            10.1080/21592799.2017.1301151. eCollection 2017"
COMMENT     Sequence Label: Rab14 cds in pGem-T Easy
FEATURES             Location/Qualifiers
     source          1..3682
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     regulatory      392..401
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     regulatory      396..405
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     primer_bind     complement(435..454)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(436..454)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(461..478)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(461..477)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(470..492)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      618..1073
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(705..724)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     915..936
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        1151..1255
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(1256..1324,1325..2116)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(1474..1493)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      2287..2875
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     2776..2795
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     3029..3046
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    3163..3184
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(3199..3204,3205..3222,3223..3229)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    3237..3253
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     3242..3264
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     3261..3277
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     3261..3277
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     promoter        3295..3313
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     3295..3312
                     /label=SP6
                     /note="SP6 promoter, forward primer"
ORIGIN
        1 ttgttaccga ttagaatgat gacagtattg gggttggtga gattcctggc atcagtcagc
       61 cagctgctaa ggtgattgta cgtgcttcgc ctggtgatgt catacaccat gagggcccca
      121 gcggctcctc tgtagtagct tcgtgtgaca gcacgaaacc tctcctgacc tgccgtgtcc
      181 caaatctgaa gcttcacctt ctgcccactc acttcaatta tccgtgtgcc aaactcaaca
      241 ccgattgtat gaggacagtc agccataaat ttcttttctg tgaactggtg gagtaaacag
      301 gacttgccaa cccccatgtc accaataatg atatatttga aaatatagga atagttatat
      361 ggtgcggtcg tcatttaatc gaattcccgc ggccgccatg gcggccggga gcatgcgacg
      421 tcgggcccaa ttcgccctat agtgagtcgt attacaattc actggccgtc gttttacaac
      481 gtcgtgactg ggaaaaccct ggcgttaccc aacttaatcg ccttgcagca catccccctt
      541 tcgccagctg gcgtaatagc gaagaggccc gcaccgatcg cccttcccaa cagttgcgca
      601 gcctgaatgg cgaatggacg cgccctgtag cggcgcatta agcgcggcgg gtgtggtggt
      661 tacgcgcagc gtgaccgcta cacttgccag cgccctagcg cccgctcctt tcgctttctt
      721 cccttccttt ctcgccacgt tcgccggctt tccccgtcaa gctctaaatc gggggctccc
      781 tttagggttc cgatttagtg ctttacggca cctcgacccc aaaaaacttg attagggtga
      841 tggttcacgt agtgggccat cgccctgata gacggttttt cgccctttga cgttggagtc
      901 cacgttcttt aatagtggac tcttgttcca aactggaaca acactcaacc ctatctcggt
      961 ctattctttt gatttataag ggattttgcc gatttcggcc tattggttaa aaaatgagct
     1021 gatttaacaa aaatttaacg cgaattttaa caaaatatta acgcttacaa tttcctgatg
     1081 cggtattttc tccttacgca tctgtgcggt atttcacacc gcatcaggtg gcacttttcg
     1141 gggaaatgtg cgcggaaccc ctatttgttt atttttctaa atacattcaa atatgtatcc
     1201 gctcatgaga caataaccct gataaatgct tcaataatat tgaaaaagga agagtatgag
     1261 tattcaacat ttccgtgtcg cccttattcc cttttttgcg gcattttgcc ttcctgtttt
     1321 tgctcaccca gaaacgctgg tgaaagtaaa agatgctgaa gatcagttgg gtgcacgagt
     1381 gggttacatc gaactggatc tcaacagcgg taagatcctt gagagttttc gccccgaaga
     1441 acgttttcca atgatgagca cttttaaagt tctgctatgt ggcgcggtat tatcccgtat
     1501 tgacgccggg caagagcaac tcggtcgccg catacactat tctcagaatg acttggttga
     1561 gtactcacca gtcacagaaa agcatcttac ggatggcatg acagtaagag aattatgcag
     1621 tgctgccata accatgagtg ataacactgc ggccaactta cttctgacaa cgatcggagg
     1681 accgaaggag ctaaccgctt ttttgcacaa catgggggat catgtaactc gccttgatcg
     1741 ttgggaaccg gagctgaatg aagccatacc aaacgacgag cgtgacacca cgatgcctgt
     1801 agcaatggca acaacgttgc gcaaactatt aactggcgaa ctacttactc tagcttcccg
     1861 gcaacaatta atagactgga tggaggcgga taaagttgca ggaccacttc tgcgctcggc
     1921 ccttccggct ggctggttta ttgctgataa atctggagcc ggtgagcgtg ggtctcgcgg
     1981 tatcattgca gcactggggc cagatggtaa gccctcccgt atcgtagtta tctacacgac
     2041 ggggagtcag gcaactatgg atgaacgaaa tagacagatc gctgagatag gtgcctcact
     2101 gattaagcat tggtaactgt cagaccaagt ttactcatat atactttaga ttgatttaaa
     2161 acttcatttt taatttaaaa ggatctaggt gaagatcctt tttgataatc tcatgaccaa
     2221 aatcccttaa cgtgagtttt cgttccactg agcgtcagac cccgtagaaa agatcaaagg
     2281 atcttcttga gatccttttt ttctgcgcgt aatctgctgc ttgcaaacaa aaaaaccacc
     2341 gctaccagcg gtggtttgtt tgccggatca agagctacca actctttttc cgaaggtaac
     2401 tggcttcagc agagcgcaga taccaaatac tgttcttcta gtgtagccgt agttaggcca
     2461 ccacttcaag aactctgtag caccgcctac atacctcgct ctgctaatcc tgttaccagt
     2521 ggctgctgcc agtggcgata agtcgtgtct taccgggttg gactcaagac gatagttacc
     2581 ggataaggcg cagcggtcgg gctgaacggg gggttcgtgc acacagccca gcttggagcg
     2641 aacgacctac accgaactga gatacctaca gcgtgagcta tgagaaagcg ccacgcttcc
     2701 cgaagggaga aaggcggaca ggtatccggt aagcggcagg gtcggaacag gagagcgcac
     2761 gagggagctt ccagggggaa acgcctggta tctttatagt cctgtcgggt ttcgccacct
     2821 ctgacttgag cgtcgatttt tgtgatgctc gtcagggggg cggagcctat ggaaaaacgc
     2881 cagcaacgcg gcctttttac ggttcctggc cttttgctgg ccttttgctc acatgttctt
     2941 tcctgcgtta tcccctgatt ctgtggataa ccgtattacc gcctttgagt gagctgatac
     3001 cgctcgccgc agccgaacga ccgagcgcag cgagtcagtg agcgaggaag cggaagagcg
     3061 cccaatacgc aaaccgcctc tccccgcgcg ttggccgatt cattaatgca gctggcacga
     3121 caggtttccc gactggaaag cgggcagtga gcgcaacgca attaatgtga gttagctcac
     3181 tcattaggca ccccaggctt tacactttat gcttccggct cgtatgttgt gtggaattgt
     3241 gagcggataa caatttcaca caggaaacag ctatgaccat gattacgcca agctatttag
     3301 gtgacactat agaatactca agctatgcat ccaacgcgtt gggagctctc ccatatggtc
     3361 gacctgcagg cggccgcgaa ttcactagtg attaagtctc catggtcatt aacagctgca
     3421 gccctctttc tggggctgtg cgtcgctgct gagccgccct ccctgcgggg cggtgggctt
     3481 gtgctggacc cctgactcgg ctgcgttcag gtccaggctg ccgtcctgaa tgttctggta
     3541 gatcttcttg gcagcttcca gaaaagcatc ctccacattc tcgcctgttt ttgcacttgc
     3601 ctcaaggaac aacaaaccgt tctcttcagc aaactgcttg gcctcttcat atgtgacatc
     3661 acgctgggct tctaggtctg ct
//
