LOCUS       sequence-176722-        3701 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Can be used for in situ hybridisation or other purposes.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3701)
  AUTHORS   Hall TE, Martel N, Lo HP, Xiong Z, Parton RG
  TITLE     A plasmid library of full-length zebrafish rab proteins for in vivo 
            cell biology.
  JOURNAL   Cell Logist. 2017 Mar 1;7(1):e1301151. doi: 
            10.1080/21592799.2017.1301151. eCollection 2017.
  PUBMED    28396820
REFERENCE   2  (bases 1 to 3701)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell 
            Logist."; date: "2017-03-1"; volume: "7(1):e1301151. doi"; pages: " 
            10.1080/21592799.2017.1301151. eCollection 2017"
COMMENT     Sequence Label: Rab33bb cds in pGem-T Easy
FEATURES             Location/Qualifiers
     source          1..3701
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      complement(141..729)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(221..240)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(900..1691,1692..1760))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     1523..1542
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(1761..1865)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      complement(1943..2398)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(2080..2101)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     2292..2311
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     2524..2546
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     2538..2555
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     2539..2555
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     2562..2581
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        2562..2580
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     regulatory      2611..2620
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     regulatory      2615..2624
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     promoter        complement(3404..3422)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(3405..3422)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     complement(3440..3456)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(3440..3456)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(3453..3475)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    3464..3480
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(3488..3494,3495..3512,3513..3518))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    3533..3554
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(3671..3688)
                     /label=L4440
                     /note="L4440 vector, forward primer"
ORIGIN
        1 cggctgcggc gagcggtatc agctcactca aaggcggtaa tacggttatc cacagaatca
       61 ggggataacg caggaaagaa catgtgagca aaaggccagc aaaaggccag gaaccgtaaa
      121 aaggccgcgt tgctggcgtt tttccatagg ctccgccccc ctgacgagca tcacaaaaat
      181 cgacgctcaa gtcagaggtg gcgaaacccg acaggactat aaagatacca ggcgtttccc
      241 cctggaagct ccctcgtgcg ctctcctgtt ccgaccctgc cgcttaccgg atacctgtcc
      301 gcctttctcc cttcgggaag cgtggcgctt tctcatagct cacgctgtag gtatctcagt
      361 tcggtgtagg tcgttcgctc caagctgggc tgtgtgcacg aaccccccgt tcagcccgac
      421 cgctgcgcct tatccggtaa ctatcgtctt gagtccaacc cggtaagaca cgacttatcg
      481 ccactggcag cagccactgg taacaggatt agcagagcga ggtatgtagg cggtgctaca
      541 gagttcttga agtggtggcc taactacggc tacactagaa gaacagtatt tggtatctgc
      601 gctctgctga agccagttac cttcggaaaa agagttggta gctcttgatc cggcaaacaa
      661 accaccgctg gtagcggtgg tttttttgtt tgcaagcagc agattacgcg cagaaaaaaa
      721 ggatctcaag aagatccttt gatcttttct acggggtctg acgctcagtg gaacgaaaac
      781 tcacgttaag ggattttggt catgagatta tcaaaaagga tcttcaccta gatcctttta
      841 aattaaaaat gaagttttaa atcaatctaa agtatatatg agtaaacttg gtctgacagt
      901 taccaatgct taatcagtga ggcacctatc tcagcgatct gtctatttcg ttcatccata
      961 gttgcctgac tccccgtcgt gtagataact acgatacggg agggcttacc atctggcccc
     1021 agtgctgcaa tgataccgcg agacccacgc tcaccggctc cagatttatc agcaataaac
     1081 cagccagccg gaagggccga gcgcagaagt ggtcctgcaa ctttatccgc ctccatccag
     1141 tctattaatt gttgccggga agctagagta agtagttcgc cagttaatag tttgcgcaac
     1201 gttgttgcca ttgctacagg catcgtggtg tcacgctcgt cgtttggtat ggcttcattc
     1261 agctccggtt cccaacgatc aaggcgagtt acatgatccc ccatgttgtg caaaaaagcg
     1321 gttagctcct tcggtcctcc gatcgttgtc agaagtaagt tggccgcagt gttatcactc
     1381 atggttatgg cagcactgca taattctctt actgtcatgc catccgtaag atgcttttct
     1441 gtgactggtg agtactcaac caagtcattc tgagaatagt gtatgcggcg accgagttgc
     1501 tcttgcccgg cgtcaatacg ggataatacc gcgccacata gcagaacttt aaaagtgctc
     1561 atcattggaa aacgttcttc ggggcgaaaa ctctcaagga tcttaccgct gttgagatcc
     1621 agttcgatgt aacccactcg tgcacccaac tgatcttcag catcttttac tttcaccagc
     1681 gtttctgggt gagcaaaaac aggaaggcaa aatgccgcaa aaaagggaat aagggcgaca
     1741 cggaaatgtt gaatactcat actcttcctt tttcaatatt attgaagcat ttatcagggt
     1801 tattgtctca tgagcggata catatttgaa tgtatttaga aaaataaaca aataggggtt
     1861 ccgcgcacat ttccccgaaa agtgccacct gatgcggtgt gaaataccgc acagatgcgt
     1921 aaggagaaaa taccgcatca ggaaattgta agcgttaata ttttgttaaa attcgcgtta
     1981 aatttttgtt aaatcagctc attttttaac caataggccg aaatcggcaa aatcccttat
     2041 aaatcaaaag aatagaccga gatagggttg agtgttgttc cagtttggaa caagagtcca
     2101 ctattaaaga acgtggactc caacgtcaaa gggcgaaaaa ccgtctatca gggcgatggc
     2161 ccactacgtg aaccatcacc ctaatcaagt tttttggggt cgaggtgccg taaagcacta
     2221 aatcggaacc ctaaagggag cccccgattt agagcttgac ggggaaagcc ggcgaacgtg
     2281 gcgagaaagg aagggaagaa agcgaaagga gcgggcgcta gggcgctggc aagtgtagcg
     2341 gtcacgctgc gcgtaaccac cacacccgcc gcgcttaatg cgccgctaca gggcgcgtcc
     2401 attcgccatt caggctgcgc aactgttggg aagggcgatc ggtgcgggcc tcttcgctat
     2461 tacgccagct ggcgaaaggg ggatgtgctg caaggcgatt aagttgggta acgccagggt
     2521 tttcccagtc acgacgttgt aaaacgacgg ccagtgaatt gtaatacgac tcactatagg
     2581 gcgaattggg cccgacgtcg catgctcccg gccgccatgg cggccgcggg aattcgatta
     2641 tggattcgtc tttagaaagc tccagctcct ctaacggccc cccgacgcgc tgctgtcgga
     2701 cctgtaagat catcgtgatc ggggatgccg gtgtgggcaa gacctgtctg acacatcgat
     2761 tctgcaccgg acaatttcct accagaacag aggcgaccat cggggtggat tttcgagaga
     2821 aacttcttca gatagacggc gaaaaaataa aagtacagct atgggacacg gccggtcagg
     2881 agcgcttccg taaaagcatg gtgcagcact actatcgaaa tgtgcatggt atcgtcttcg
     2941 tctttgacgt cacaaaccaa tccagtttct gcaatctgcc tttgtggatg gacgagtgcc
     3001 gtcagcactc tcttggtctg gaaattccac gtgccctagt ttgcaacaag gctgacctga
     3061 tgtcttcaaa tgcttctgtg ctaatcgagc aggctcgtca tttggctgaa gtccacggga
     3121 tgtcttttta tctcacttca gccaaaggtc ttaaaggcga ccaggtggac agcatcttca
     3181 tggctttagc acagaggctg aagagccaga gaagggagag cgtaaatgga cgtgtgctag
     3241 agtgcagagc tgagagctta aaaatccagg cacgggttat aacagcccaa gagactaaaa
     3301 agaaatggac ctgcatctgc taaaatcact agtgaattcg cggccgcctg caggtcgacc
     3361 atatgggaga gctcccaacg cgttggatgc atagcttgag tattctatag tgtcacctaa
     3421 atagcttggc gtaatcatgg tcatagctgt ttcctgtgtg aaattgttat ccgctcacaa
     3481 ttccacacaa catacgagcc ggaagcataa agtgtaaagc ctggggtgcc taatgagtga
     3541 gctaactcac attaattgcg ttgcgctcac tgcccgcttt ccagtcggga aacctgtcgt
     3601 gccagctgca ttaatgaatc ggccaacgcg cggggagagg cggtttgcgt attgggcgct
     3661 cttccgcttc ctcgctcact gactcgctgc gctcggtcgt t
//
