LOCUS       sequence-176731-        3650 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Can be used for in situ hybridisation or other purposes.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3650)
  AUTHORS   Hall TE, Martel N, Lo HP, Xiong Z, Parton RG
  TITLE     A plasmid library of full-length zebrafish rab proteins for in vivo 
            cell biology.
  JOURNAL   Cell Logist. 2017 Mar 1;7(1):e1301151. doi: 
            10.1080/21592799.2017.1301151. eCollection 2017.
  PUBMED    28396820
REFERENCE   2  (bases 1 to 3650)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell 
            Logist."; date: "2017-03-1"; volume: "7(1):e1301151. doi"; pages: " 
            10.1080/21592799.2017.1301151. eCollection 2017"
COMMENT     Sequence Label: Rab38b cds in pGem-T Easy
FEATURES             Location/Qualifiers
     source          1..3650
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     192..214
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     206..223
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     207..223
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     230..249
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        230..248
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     regulatory      279..288
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     regulatory      283..292
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     promoter        complement(1021..1039)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(1022..1039)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     complement(1057..1073)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(1057..1073)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(1070..1092)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    1081..1097
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(1105..1111,1112..1129,1130..1135))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    1150..1171
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(1288..1305)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(1459..2047)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(1539..1558)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(2218..3009,3010..3078))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     2841..2860
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(3079..3183)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      complement(join(3261..3650,1..66))
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(3398..3419)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     3610..3629
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
ORIGIN
        1 gtgtagcggt cacgctgcgc gtaaccacca cacccgccgc gcttaatgcg ccgctacagg
       61 gcgcgtccat tcgccattca ggctgcgcaa ctgttgggaa gggcgatcgg tgcgggcctc
      121 ttcgctatta cgccagctgg cgaaaggggg atgtgctgca aggcgattaa gttgggtaac
      181 gccagggttt tcccagtcac gacgttgtaa aacgacggcc agtgaattgt aatacgactc
      241 actatagggc gaattgggcc cgacgtcgca tgctcccggc cgccatggcg gccgcgggaa
      301 ttcgattatg cataacaatc agaaggagca tttgtataaa attcttgtca tcggtgattt
      361 gggagtaggg aagaccagca tcattaagcg ttatgtgcac cagacgtact ccaccaacta
      421 cagagccacc ataggcgtgg atttcgcgct caaagttctg aactgggact cggagacggt
      481 ccggctgcag ctttgggaca tcgcaggtca ggagcggttc ggcaacatga cgcgggttta
      541 ttacagagag gccatggggg cattcattgt gtttgatgtt accagacccg ccacgtttga
      601 ggcagtctcc aaatggaagg aggatctgga ctctaaatta atactttcaa atggccagag
      661 tattgccaca gtgctcctgg ccaacaagtg cgaccaaaac agagacttcc tgactaacaa
      721 tggcctgaaa atggaccagt actgcaggga gaatggattt gtggggtggt ttgagacatc
      781 tgctaaggaa aacgtcaaca ttaatgatgc agccaacttc cttgtgaagc acataattgc
      841 cagtgagaac gatatcctga aatcagtggt tccagatacc atctctcctc aactcaactc
      901 ggataaagaa attacttgtt ccgcttgctt caaaccttga aatcactagt gaattcgcgg
      961 ccgcctgcag gtcgaccata tgggagagct cccaacgcgt tggatgcata gcttgagtat
     1021 tctatagtgt cacctaaata gcttggcgta atcatggtca tagctgtttc ctgtgtgaaa
     1081 ttgttatccg ctcacaattc cacacaacat acgagccgga agcataaagt gtaaagcctg
     1141 gggtgcctaa tgagtgagct aactcacatt aattgcgttg cgctcactgc ccgctttcca
     1201 gtcgggaaac ctgtcgtgcc agctgcatta atgaatcggc caacgcgcgg ggagaggcgg
     1261 tttgcgtatt gggcgctctt ccgcttcctc gctcactgac tcgctgcgct cggtcgttcg
     1321 gctgcggcga gcggtatcag ctcactcaaa ggcggtaata cggttatcca cagaatcagg
     1381 ggataacgca ggaaagaaca tgtgagcaaa aggccagcaa aaggccagga accgtaaaaa
     1441 ggccgcgttg ctggcgtttt tccataggct ccgcccccct gacgagcatc acaaaaatcg
     1501 acgctcaagt cagaggtggc gaaacccgac aggactataa agataccagg cgtttccccc
     1561 tggaagctcc ctcgtgcgct ctcctgttcc gaccctgccg cttaccggat acctgtccgc
     1621 ctttctccct tcgggaagcg tggcgctttc tcatagctca cgctgtaggt atctcagttc
     1681 ggtgtaggtc gttcgctcca agctgggctg tgtgcacgaa ccccccgttc agcccgaccg
     1741 ctgcgcctta tccggtaact atcgtcttga gtccaacccg gtaagacacg acttatcgcc
     1801 actggcagca gccactggta acaggattag cagagcgagg tatgtaggcg gtgctacaga
     1861 gttcttgaag tggtggccta actacggcta cactagaaga acagtatttg gtatctgcgc
     1921 tctgctgaag ccagttacct tcggaaaaag agttggtagc tcttgatccg gcaaacaaac
     1981 caccgctggt agcggtggtt tttttgtttg caagcagcag attacgcgca gaaaaaaagg
     2041 atctcaagaa gatcctttga tcttttctac ggggtctgac gctcagtgga acgaaaactc
     2101 acgttaaggg attttggtca tgagattatc aaaaaggatc ttcacctaga tccttttaaa
     2161 ttaaaaatga agttttaaat caatctaaag tatatatgag taaacttggt ctgacagtta
     2221 ccaatgctta atcagtgagg cacctatctc agcgatctgt ctatttcgtt catccatagt
     2281 tgcctgactc cccgtcgtgt agataactac gatacgggag ggcttaccat ctggccccag
     2341 tgctgcaatg ataccgcgag acccacgctc accggctcca gatttatcag caataaacca
     2401 gccagccgga agggccgagc gcagaagtgg tcctgcaact ttatccgcct ccatccagtc
     2461 tattaattgt tgccgggaag ctagagtaag tagttcgcca gttaatagtt tgcgcaacgt
     2521 tgttgccatt gctacaggca tcgtggtgtc acgctcgtcg tttggtatgg cttcattcag
     2581 ctccggttcc caacgatcaa ggcgagttac atgatccccc atgttgtgca aaaaagcggt
     2641 tagctccttc ggtcctccga tcgttgtcag aagtaagttg gccgcagtgt tatcactcat
     2701 ggttatggca gcactgcata attctcttac tgtcatgcca tccgtaagat gcttttctgt
     2761 gactggtgag tactcaacca agtcattctg agaatagtgt atgcggcgac cgagttgctc
     2821 ttgcccggcg tcaatacggg ataataccgc gccacatagc agaactttaa aagtgctcat
     2881 cattggaaaa cgttcttcgg ggcgaaaact ctcaaggatc ttaccgctgt tgagatccag
     2941 ttcgatgtaa cccactcgtg cacccaactg atcttcagca tcttttactt tcaccagcgt
     3001 ttctgggtga gcaaaaacag gaaggcaaaa tgccgcaaaa aagggaataa gggcgacacg
     3061 gaaatgttga atactcatac tcttcctttt tcaatattat tgaagcattt atcagggtta
     3121 ttgtctcatg agcggataca tatttgaatg tatttagaaa aataaacaaa taggggttcc
     3181 gcgcacattt ccccgaaaag tgccacctga tgcggtgtga aataccgcac agatgcgtaa
     3241 ggagaaaata ccgcatcagg aaattgtaag cgttaatatt ttgttaaaat tcgcgttaaa
     3301 tttttgttaa atcagctcat tttttaacca ataggccgaa atcggcaaaa tcccttataa
     3361 atcaaaagaa tagaccgaga tagggttgag tgttgttcca gtttggaaca agagtccact
     3421 attaaagaac gtggactcca acgtcaaagg gcgaaaaacc gtctatcagg gcgatggccc
     3481 actacgtgaa ccatcaccct aatcaagttt tttggggtcg aggtgccgta aagcactaaa
     3541 tcggaaccct aaagggagcc cccgatttag agcttgacgg ggaaagccgg cgaacgtggc
     3601 gagaaaggaa gggaagaaag cgaaaggagc gggcgctagg gcgctggcaa
//
