LOCUS       sequence-176736-        3795 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Can be used for in situ hybridisation or other purposes.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3795)
  AUTHORS   Hall TE, Martel N, Lo HP, Xiong Z, Parton RG
  TITLE     A plasmid library of full-length zebrafish rab proteins for in vivo 
            cell biology.
  JOURNAL   Cell Logist. 2017 Mar 1;7(1):e1301151. doi: 
            10.1080/21592799.2017.1301151. eCollection 2017.
  PUBMED    28396820
REFERENCE   2  (bases 1 to 3795)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell 
            Logist."; date: "2017-03-1"; volume: "7(1):e1301151. doi"; pages: " 
            10.1080/21592799.2017.1301151. eCollection 2017"
COMMENT     Sequence Label: Rab34b cds in pGem-T Easy
FEATURES             Location/Qualifiers
     source          1..3795
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     345..364
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     598..615
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    732..753
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(768..773,774..791,792..798)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    806..822
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     811..833
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     830..846
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     830..846
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     promoter        864..882
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     864..881
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     regulatory      1756..1765
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     regulatory      1760..1769
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     primer_bind     complement(1799..1818)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(1800..1818)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(1825..1842)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(1825..1841)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(1834..1856)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      1982..2437
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(2069..2088)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     2279..2300
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        2515..2619
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(2620..2688,2689..3480)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(2838..2857)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      join(3651..3795,1..444)
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
ORIGIN
        1 gttcttctag tgtagccgta gttaggccac cacttcaaga actctgtagc accgcctaca
       61 tacctcgctc tgctaatcct gttaccagtg gctgctgcca gtggcgataa gtcgtgtctt
      121 accgggttgg actcaagacg atagttaccg gataaggcgc agcggtcggg ctgaacgggg
      181 ggttcgtgca cacagcccag cttggagcga acgacctaca ccgaactgag atacctacag
      241 cgtgagctat gagaaagcgc cacgcttccc gaagggagaa aggcggacag gtatccggta
      301 agcggcaggg tcggaacagg agagcgcacg agggagcttc cagggggaaa cgcctggtat
      361 ctttatagtc ctgtcgggtt tcgccacctc tgacttgagc gtcgattttt gtgatgctcg
      421 tcaggggggc ggagcctatg gaaaaacgcc agcaacgcgg cctttttacg gttcctggcc
      481 ttttgctggc cttttgctca catgttcttt cctgcgttat cccctgattc tgtggataac
      541 cgtattaccg cctttgagtg agctgatacc gctcgccgca gccgaacgac cgagcgcagc
      601 gagtcagtga gcgaggaagc ggaagagcgc ccaatacgca aaccgcctct ccccgcgcgt
      661 tggccgattc attaatgcag ctggcacgac aggtttcccg actggaaagc gggcagtgag
      721 cgcaacgcaa ttaatgtgag ttagctcact cattaggcac cccaggcttt acactttatg
      781 cttccggctc gtatgttgtg tggaattgtg agcggataac aatttcacac aggaaacagc
      841 tatgaccatg attacgccaa gctatttagg tgacactata gaatactcaa gctatgcatc
      901 caacgcgttg ggagctctcc catatggtcg acctgcaggc ggccgcgaat tcactagtga
      961 ttatgttcag tcaatggcag cagctggatt gcctcttctt aaaattgttg tcacaattat
     1021 tggagatttt gacgatatca ccggctcgtc tagaattgtt cttctccagc tcagataaaa
     1081 cagtggcctc aaatgtaagc gaagcaaccc gcaggaagaa atctctcaca ctcgcccctg
     1141 acaaagcaga aactgcccag tactctgctc tgatctcctc agacaatcga atggcctcct
     1201 gctccatttg ggccaacaga tctggatgac taagatcctt cttggttccc acgaggaaga
     1261 gcaggacact ggacgggtca ttgtccctca tggcatcttc aagccattct ctcgcgtgat
     1321 ccagagactc atagttgctc aggtcaaaca caacgatgat ggcttgcgct cctctgtagt
     1381 atgtggaggc aatgcatcga aatctttcct gacctgctgt gtcccacagc tgcaaactga
     1441 agggtacccc gagaacttca aatctctcca tctcaaaatc cactccaata gttgccttgt
     1501 acgttctgtc aaatgagtct ttgcagaatc tgtttattaa acaggtcttc ccgaccccaa
     1561 catcaccaac gacaatgact ttggctacat ttaatcctgt tttctgccct tgacaagcct
     1621 ctctaacagt aatattgaag ccatctttgg tgtgcactgc agcatctctt gtaaaataca
     1681 aagggagttc acagatgatc cggtctcctt tcacaggggg taaagaattc atcatgtgag
     1741 aatcgaattc ccgcggccgc catggcggcc gggagcatgc gacgtcgggc ccaattcgcc
     1801 ctatagtgag tcgtattaca attcactggc cgtcgtttta caacgtcgtg actgggaaaa
     1861 ccctggcgtt acccaactta atcgccttgc agcacatccc cctttcgcca gctggcgtaa
     1921 tagcgaagag gcccgcaccg atcgcccttc ccaacagttg cgcagcctga atggcgaatg
     1981 gacgcgccct gtagcggcgc attaagcgcg gcgggtgtgg tggttacgcg cagcgtgacc
     2041 gctacacttg ccagcgccct agcgcccgct cctttcgctt tcttcccttc ctttctcgcc
     2101 acgttcgccg gctttccccg tcaagctcta aatcgggggc tccctttagg gttccgattt
     2161 agtgctttac ggcacctcga ccccaaaaaa cttgattagg gtgatggttc acgtagtggg
     2221 ccatcgccct gatagacggt ttttcgccct ttgacgttgg agtccacgtt ctttaatagt
     2281 ggactcttgt tccaaactgg aacaacactc aaccctatct cggtctattc ttttgattta
     2341 taagggattt tgccgatttc ggcctattgg ttaaaaaatg agctgattta acaaaaattt
     2401 aacgcgaatt ttaacaaaat attaacgctt acaatttcct gatgcggtat tttctcctta
     2461 cgcatctgtg cggtatttca caccgcatca ggtggcactt ttcggggaaa tgtgcgcgga
     2521 acccctattt gtttattttt ctaaatacat tcaaatatgt atccgctcat gagacaataa
     2581 ccctgataaa tgcttcaata atattgaaaa aggaagagta tgagtattca acatttccgt
     2641 gtcgccctta ttcccttttt tgcggcattt tgccttcctg tttttgctca cccagaaacg
     2701 ctggtgaaag taaaagatgc tgaagatcag ttgggtgcac gagtgggtta catcgaactg
     2761 gatctcaaca gcggtaagat ccttgagagt tttcgccccg aagaacgttt tccaatgatg
     2821 agcactttta aagttctgct atgtggcgcg gtattatccc gtattgacgc cgggcaagag
     2881 caactcggtc gccgcataca ctattctcag aatgacttgg ttgagtactc accagtcaca
     2941 gaaaagcatc ttacggatgg catgacagta agagaattat gcagtgctgc cataaccatg
     3001 agtgataaca ctgcggccaa cttacttctg acaacgatcg gaggaccgaa ggagctaacc
     3061 gcttttttgc acaacatggg ggatcatgta actcgccttg atcgttggga accggagctg
     3121 aatgaagcca taccaaacga cgagcgtgac accacgatgc ctgtagcaat ggcaacaacg
     3181 ttgcgcaaac tattaactgg cgaactactt actctagctt cccggcaaca attaatagac
     3241 tggatggagg cggataaagt tgcaggacca cttctgcgct cggcccttcc ggctggctgg
     3301 tttattgctg ataaatctgg agccggtgag cgtgggtctc gcggtatcat tgcagcactg
     3361 gggccagatg gtaagccctc ccgtatcgta gttatctaca cgacggggag tcaggcaact
     3421 atggatgaac gaaatagaca gatcgctgag ataggtgcct cactgattaa gcattggtaa
     3481 ctgtcagacc aagtttactc atatatactt tagattgatt taaaacttca tttttaattt
     3541 aaaaggatct aggtgaagat cctttttgat aatctcatga ccaaaatccc ttaacgtgag
     3601 ttttcgttcc actgagcgtc agaccccgta gaaaagatca aaggatcttc ttgagatcct
     3661 ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc agcggtggtt
     3721 tgtttgccgg atcaagagct accaactctt tttccgaagg taactggctt cagcagagcg
     3781 cagataccaa atact
//
