LOCUS       sequence-176739-       10570 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Vector for reporter assay of one zebrafish super-enhancer region.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10570)
  AUTHORS   Perez-Rico YA, Boeva V, Mallory AC, Bitetti A, Majello S, Barillot 
            E, Shkumatava A
  TITLE     Comparative analyses of super-enhancers reveal conserved elements in
            vertebrate genomes.
  JOURNAL   Genome Res. 2017 Feb;27(2):259-268. doi: 10.1101/gr.203679.115. Epub
            2016 Dec 13.
  PUBMED    27965291
REFERENCE   2  (bases 1 to 10570)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1101/gr.203679.115"; journalName: "Genome Res"; date: "2017-02";
            volume: "27"; issue: "2"; pages: "259-268"
COMMENT     Sequence Label: E1b-GFP-Tol2-Gateway dre SE-irf2bpl D
FEATURES             Location/Qualifiers
     source          1..10570
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    complement(1..125)
                     /gene="mutant version of attR"
                     /label=attR2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     intron          1482..2054
                     /label=beta-globin intron
                     /note="intron from rabbit beta-globin gene"
     regulatory      2128..2137
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(2134..2136,2137..2139,2140..2853)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             join(2134..2136,2137..2139,2140..2850)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(2179..2200)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(2440..2459)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     2787..2808
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     polyA_signal    2898..3032
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(2922..2941)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     2976..2995
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     misc_feature    join(3469..3656,3657..3668)
                     /label=Tol2 left end
                     /note="left end of the Tol2 transposon from the medaka fish
                     Oryzias latipes (Kawakami, 2007)"
     primer_bind     complement(3702..3718)
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(3702..3718)
                     /label=pBluescriptSK
                     /note="For pBluescript vector"
     primer_bind     complement(3755..3775)
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        complement(3755..3773)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(3794..3810)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(3794..3810)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(3807..3829)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    3818..3834
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(3842..3848,3849..3866,3867..3872))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    3887..3908
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(4025..4042)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(4196..4784)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(4276..4295)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(4955..5746,5747..5815))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     5578..5597
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(5816..5920)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      complement(5946..6401)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(6083..6104)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     6295..6314
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     6528..6550
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     6542..6559
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     6543..6559
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     6569..6588
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        6569..6587
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     misc_feature    join(8438..8449,8450..8612)
                     /label=Tol2 right end
                     /note="right end of the Tol2 transposon from the medaka 
                     fish Oryzias latipes (Kawakami, 2007)"
     protein_bind    8991..9115
                     /gene="mutant version of attR"
                     /label=attR1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     promoter        join(9140..9145,9146..9163,9164..9170)
                     /label=lac UV5 promoter
                     /note="E. coli lac promoter with an ""up"" mutation"
     CDS             9224..9883
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     complement(9290..9309)
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     CDS             10225..10530
                     /codon_start=1
                     /gene="ccdB"
                     /product="CcdB, a bacterial toxin that poisons DNA gyrase"
                     /label=ccdB
                     /note="Plasmids containing the ccdB gene cannot be 
                     propagated in standard E. coli strains."
                     /translation="MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDK
                     VSRELYPVVHIGDESWRMMTTDMASVPVSVIGEEVADLSHRENDIKNAINLMFWGI"
     primer_bind     10409..10428
                     /label=ccdB-fwd
                     /note="ccdB gene, forward primer"
ORIGIN
        1 catagtgact ggatatgttg tgttttacag tattatgtag tctgtttttt atgcaaaatc
       61 taatttaata tattgatatt tatatcattt tacgtttctc gttcagcttt cttgtacaaa
      121 gtggtgatat caagatctga gaaggataca gacaagcaat caccaataca gaacgtctca
      181 atgacatctt ctgttgtaaa aaataataat aaaagaacgt ttggcagaaa tgcctttttt
      241 acacagcaga tttgcatttg ggatttaatt ccaacagtaa tgtccaaaaa cgctttataa
      301 tcctgatcat tattaagaaa acagcacaag aataatcaca aagaaaaacc tattcccatt
      361 atataaagaa aacaaagcat acggtaaaac aggaacattt ctataaacac cagcattcag
      421 acagccaaat gctggcacac gcagggttaa cgctgaaccc ctccgctctt tgtgtccgca
      481 cattcctgaa taaatgatgt tttgaacaag gctgttacca tgggcaactg tgcacgatgg
      541 cactgcagcg taacctttgg agtggctgta tttttatccg ctggaaactt ctcccttgaa
      601 gggagggagg gagagaagtg cgtgtgtgtg tgtgtgtgat ggggatgaag ggaattggag
      661 gaggggaaga agctgctggc atccctagct agcgaagggt ggacagactg cagtgatttt
      721 actatgcaac caaacacaaa tgttccaaaa acagacgagc taccatgtta aaggagcatt
      781 tattcggaaa cgattgcgtc agcactaata aaatcagtgc aaatgcattt taacaacaca
      841 aacattgtcg aaaaattgga acacaaaggc agtgactgga ttttcagggt cttgcagtta
      901 ttaatatgtt acaaagaaca tatccgttta caacggcaac tcagaaacac ctaaacatct
      961 ctgcatttac aaggatcata attattcaac tgtagtggct tctccacatc tccgagttgt
     1021 cggtatccaa taaagcattg caagaaaagg tgcagaattt aacacacaaa ctcgagacac
     1081 cacagaagcg tgtgtgtgta gtaaacagga agtgagaccc ccgccttctc taatgggtgg
     1141 ggttggtgga tctgtttatt ttctttttct ttgtatgtgc gctgcaactt gaaacccagg
     1201 tgattacgga ggcagccaag ggcacctcaa aaccaagtca gttttagcat gacaatagaa
     1261 gcccactcca tcctgaacca gacaccacac ctttgattcc tcacagacag agcagcagcg
     1321 acaataacac tgcaatgcac cacaaagcac gacaactaaa ctcttcactg gcttcagatg
     1381 gagatctctc gactctagag ggtatataat ggatcccatc gcgtctcagc ctcactttga
     1441 gctcctccac acgaatttcg accgatcctg agaacttcag ggtgagtttg gggacccttg
     1501 attgttcttt ctttttcgct attgtaaaat tcatgttata tggagggggc aaagttttca
     1561 gggtgttgtt tagaatggga agatgtccct tgtatcacca tggaccctca tgataatttt
     1621 gtttctttca ctttctactc tgttgacaac cattgtctcc tcttattttc ttttcatttt
     1681 ctgtaacttt ttcgttaaac tttagcttgc atttgtaacg aatttttaaa ttcacttttg
     1741 tttatttgtc agattgtaag tactttctct aatcactttt ttttcaaggc aatcagggta
     1801 tattatattg tacttcagca cagttttaga gaacaattgt tataattaaa tgataaggta
     1861 gaatatttct gcatataaat tctggctggc gtggaaatat tcttattggt agaaacaact
     1921 acaccctggt catcatcctg cctttctctt tatggttaca atgatataca ctgtttgaga
     1981 tgaggataaa atactctgag tccaaaccgg gcccctctgc taaccatgtt catgccttct
     2041 tctctttcct acagctcctg ggcaacgtgc tggttgttgt gctgtctcat cattttggca
     2101 aagaattcct cgacggatcc accggtcgcc accatggtga gcaagggcga ggagctgttc
     2161 accggggtgg tgcccatcct ggtcgagctg gacggcgacg taaacggcca caagttcagc
     2221 gtgtccggcg agggcgaggg cgatgccacc tacggcaagc tgaccctgaa gttcatctgc
     2281 accaccggca agctgcccgt gccctggccc accctcgtga ccaccctgac ctacggcgtg
     2341 cagtgcttca gccgctaccc cgaccacatg aagcagcacg acttcttcaa gtccgccatg
     2401 cccgaaggct acgtccagga gcgcaccatc ttcttcaagg acgacggcaa ctacaagacc
     2461 cgcgccgagg tgaagttcga gggcgacacc ctggtgaacc gcatcgagct gaagggcatc
     2521 gacttcaagg aggacggcaa catcctgggg cacaagctgg agtacaacta caacagccac
     2581 aacgtctata tcatggccga caagcagaag aacggcatca aggtgaactt caagatccgc
     2641 cacaacatcg aggacggcag cgtgcagctc gccgaccact accagcagaa cacccccatc
     2701 ggcgacggcc ccgtgctgct gcccgacaac cactacctga gcacccagtc cgccctgagc
     2761 aaagacccca acgagaagcg cgatcacatg gtcctgctgg agttcgtgac cgccgccggg
     2821 atcactctcg gcatggacga gctgtacaag taaagcggcc gccaccgcgg tggagctcga
     2881 attaattcat cgatgatgat ccagacatga taagatacat tgatgagttt ggacaaacca
     2941 caactagaat gcagtgaaaa aaatgcttta tttgtgaaat ttgtgatgct attgctttat
     3001 ttgtaaccat tataagctgc aataaacaag ttaacaacaa caattgcatt cattttatgt
     3061 ttcaggttca gggggaggtg tgggaggttt tttaaagcaa gtaaaacctc tacaaatgtg
     3121 gtatggctga ttatgatcct ctagatcaga tccgaagata cggccacggg tgctcttgat
     3181 cctgtggctg attttggact gtgctgctcg cagctgctga tgaatcacat acttcctcca
     3241 ttttcttcca ctgattgact gttataattt ccctaatttc caggtcaagg tgctgtgcat
     3301 tgtggtaata gatgtgacat gacgtcactt ccaaaggacc aatgaacatg tctgaccaat
     3361 ttcatataat gtgaaaacga ttttcatagg cagaataaat aacatttaaa ttaaactggg
     3421 catcagcgca attcaattgg tttggtaata gcaagggaaa atagaatgaa gtgatctcca
     3481 aaaaataagt actttttgac tgtaaataaa attgtaagga gtaaaaagta cttttttttc
     3541 taaaaaaatg taattaagta aaagtaaaag tattgatttt taattgtact caagtaaagt
     3601 aaaaatcccc aaaaataata cttaagtaca gtaatcaagt aaaattactc aagtacttta
     3661 cacctctggt tcttgacccc ctaccttcag caagcccagc agatccacta gttctagagc
     3721 ggccgccacc gcggtggagc tccagctttt gttcccttta gtgagggtta attgcgcgct
     3781 tggcgtaatc atggtcatag ctgtttcctg tgtgaaattg ttatccgctc acaattccac
     3841 acaacatacg agccggaagc ataaagtgta aagcctgggg tgcctaatga gtgagctaac
     3901 tcacattaat tgcgttgcgc tcactgcccg ctttccagtc gggaaacctg tcgtgccagc
     3961 tgcattaatg aatcggccaa cgcgcgggga gaggcggttt gcgtattggg cgctcttccg
     4021 cttcctcgct cactgactcg ctgcgctcgg tcgttcggct gcggcgagcg gtatcagctc
     4081 actcaaaggc ggtaatacgg ttatccacag aatcagggga taacgcagga aagaacatgt
     4141 gagcaaaagg ccagcaaaag gccaggaacc gtaaaaaggc cgcgttgctg gcgtttttcc
     4201 ataggctccg cccccctgac gagcatcaca aaaatcgacg ctcaagtcag aggtggcgaa
     4261 acccgacagg actataaaga taccaggcgt ttccccctgg aagctccctc gtgcgctctc
     4321 ctgttccgac cctgccgctt accggatacc tgtccgcctt tctcccttcg ggaagcgtgg
     4381 cgctttctca tagctcacgc tgtaggtatc tcagttcggt gtaggtcgtt cgctccaagc
     4441 tgggctgtgt gcacgaaccc cccgttcagc ccgaccgctg cgccttatcc ggtaactatc
     4501 gtcttgagtc caacccggta agacacgact tatcgccact ggcagcagcc actggtaaca
     4561 ggattagcag agcgaggtat gtaggcggtg ctacagagtt cttgaagtgg tggcctaact
     4621 acggctacac tagaagaaca gtatttggta tctgcgctct gctgaagcca gttaccttcg
     4681 gaaaaagagt tggtagctct tgatccggca aacaaaccac cgctggtagc ggtggttttt
     4741 ttgtttgcaa gcagcagatt acgcgcagaa aaaaaggatc tcaagaagat cctttgatct
     4801 tttctacggg gtctgacgct cagtggaacg aaaactcacg ttaagggatt ttggtcatga
     4861 gattatcaaa aaggatcttc acctagatcc ttttaaatta aaaatgaagt tttaaatcaa
     4921 tctaaagtat atatgagtaa acttggtctg acagttacca atgcttaatc agtgaggcac
     4981 ctatctcagc gatctgtcta tttcgttcat ccatagttgc ctgactcccc gtcgtgtaga
     5041 taactacgat acgggagggc ttaccatctg gccccagtgc tgcaatgata ccgcgagacc
     5101 cacgctcacc ggctccagat ttatcagcaa taaaccagcc agccggaagg gccgagcgca
     5161 gaagtggtcc tgcaacttta tccgcctcca tccagtctat taattgttgc cgggaagcta
     5221 gagtaagtag ttcgccagtt aatagtttgc gcaacgttgt tgccattgct acaggcatcg
     5281 tggtgtcacg ctcgtcgttt ggtatggctt cattcagctc cggttcccaa cgatcaaggc
     5341 gagttacatg atcccccatg ttgtgcaaaa aagcggttag ctccttcggt cctccgatcg
     5401 ttgtcagaag taagttggcc gcagtgttat cactcatggt tatggcagca ctgcataatt
     5461 ctcttactgt catgccatcc gtaagatgct tttctgtgac tggtgagtac tcaaccaagt
     5521 cattctgaga atagtgtatg cggcgaccga gttgctcttg cccggcgtca atacgggata
     5581 ataccgcgcc acatagcaga actttaaaag tgctcatcat tggaaaacgt tcttcggggc
     5641 gaaaactctc aaggatctta ccgctgttga gatccagttc gatgtaaccc actcgtgcac
     5701 ccaactgatc ttcagcatct tttactttca ccagcgtttc tgggtgagca aaaacaggaa
     5761 ggcaaaatgc cgcaaaaaag ggaataaggg cgacacggaa atgttgaata ctcatactct
     5821 tcctttttca atattattga agcatttatc agggttattg tctcatgagc ggatacatat
     5881 ttgaatgtat ttagaaaaat aaacaaatag gggttccgcg cacatttccc cgaaaagtgc
     5941 cacctaaatt gtaagcgtta atattttgtt aaaattcgcg ttaaattttt gttaaatcag
     6001 ctcatttttt aaccaatagg ccgaaatcgg caaaatccct tataaatcaa aagaatagac
     6061 cgagataggg ttgagtgttg ttccagtttg gaacaagagt ccactattaa agaacgtgga
     6121 ctccaacgtc aaagggcgaa aaaccgtcta tcagggcgat ggcccactac gtgaaccatc
     6181 accctaatca agttttttgg ggtcgaggtg ccgtaaagca ctaaatcgga accctaaagg
     6241 gagcccccga tttagagctt gacggggaaa gccggcgaac gtggcgagaa aggaagggaa
     6301 gaaagcgaaa ggagcgggcg ctagggcgct ggcaagtgta gcggtcacgc tgcgcgtaac
     6361 caccacaccc gccgcgctta atgcgccgct acagggcgcg tcccattcgc cattcaggct
     6421 gcgcaactgt tgggaagggc gatcggtgcg ggcctcttcg ctattacgcc agctggcgaa
     6481 agggggatgt gctgcaaggc gattaagttg ggtaacgcca gggttttccc agtcacgacg
     6541 ttgtaaaacg acggccagtg agcgcgcgta atacgactca ctatagggcg aattgggtac
     6601 caaatagtag gaattaccca cctgtacaag tgctgaaaac ttggatgaat aagcccgttt
     6661 gccattttct actgctattt taaatctttt ctgtatttgt ctgcatttgt ctttaccctt
     6721 gaataaatgt tttagttgtt ttttttcaat tatgactgtg tttaacagac attattacaa
     6781 taatatgtaa tagtagtaca cactattatt atgtaatagt acttgttgac tgtatttgag
     6841 aactggaccg agtgagtgtt acgtcaccca ttcaaaatga cttacttctg gctccaacaa
     6901 aatgaagtta attcagttgc catttttcac tgtatggaca tcgccgtgtt ggagctagac
     6961 gttgccaata agcaagaaag agccgagatg cgtcgagtct gagtcaccgt tcctatggca
     7021 acccctctaa ccaatcagaa gtaagcttgt tggaagtcca cagcctacca cttgaaagcg
     7081 ggctgcacaa aatctgtcaa acgttttgaa cgttggatgt gagagcacat acttttatta
     7141 aggcatctgg ttggtcagtt tataatatca acaacttggg ctacagaaaa gaaaagttat
     7201 tacagaaatt atgattaaca agtacatgtt aaataaagat tttaatatga atgccaccac
     7261 tggagcattc atgccatttg gagcttcttc ctgtttggat cactagaagg aggaggtcac
     7321 tcattacagt tctcatatac agtcgttggt tggttggttg gttggtagat tgattgattg
     7381 attgattgat tgattgattg attgattgat tgattgattg attgattgat tgattgattg
     7441 attggtagtc aaaataagaa ataatttcca cagattcatt acagaaatga ttaaatgcat
     7501 acataaaaaa ctgggggggg gggggataca acaacacact taagtcacat ttgcctacgt
     7561 aaagaaaagt aaagaaaatc aatagctata ttttacatct cctttttttg ctgtctttta
     7621 attagccttg ttttgctgtt atcttgattg aaactgtaac ttcttcacct gcttcttttc
     7681 tttgtaggtt ttgccagccc agaaacaggc atggctgtgc aaggccagag caccatgcac
     7741 aatgcgctgc atgtcttcat gaacggctca atgtcctcag tccagggctc agccaacgac
     7801 cccatcttcc tccttcacca tgctttcatt gacaggtaac aaacacgtca tgacattaga
     7861 ctgcacagtt tttgacaaag ttcatacaat ctgttgttta tagctgctac aattagtgaa
     7921 gtttgtgaat gtacttggat gagcagcgaa agatcaattg agatcaattg ttagagtttg
     7981 gttgccctgc agagcaaaga acaaaaaata atctggtggc tttactgcgt gaggttatta
     8041 ttggtggaat agaaacacaa aacataattg catttatttg tttaattttt tatcttatct
     8101 taactttcat cttgcatatt tgtttcttac atcatttcta gcatctttga gcgctggcta
     8161 agaactcatc agcctccccg gtccatctac ccacgtacca atgcaccaat tggccacaat
     8221 gacggctact acatggtgcc attccttcct ctttatagga atggagacta cctcctgtcc
     8281 aacaatgctc ttggatacga gtacgcctac ctgttggacc caggtcattg cacaacacca
     8341 gaaatgccct ctgatctgca aaagacgtga atatctgttc agacacccat atccactctg
     8401 ttccacacag gtcagaggtt tgtccaggag ttcttgacag aggtgtaaaa agtactcaaa
     8461 aattttactc aagtgaaagt acaagtactt agggaaaatt ttactcaatt aaaagtaaaa
     8521 gtatctggct agaatcttac ttgagtaaaa gtaaaaaagt actccattaa aattgtactt
     8581 gagtattaag gaagtaaaag taaaagcaag aaagaaaact agagattctt gtttaagctt
     8641 ttaatctcaa aaaacattaa atgaaatgca tacaaggttt tatcctgctt tagaactgtt
     8701 tgtatttaat tatcaaacta taagacagac aatctaatgc cagtacacgc tactcaaagt
     8761 tgtaaaacct cagatttaac ttcagtagaa gctgattctc aaaattgtta gtgtcaagcc
     8821 tagctctttt ggggctgaaa agcaatcctg cagtgctgaa aagcctctca caggcagccg
     8881 atgcgggaag aggtgtatta gtcttgatag agaggctgca aatagcagga aacgtgagca
     8941 gagactccct ggtgtctgaa acacaggcca gatgggccct cgaggatatc acaagtttgt
     9001 acaaaaaagc tgaacgagaa acgtaaaatg atataaatat caatatatta aattagattt
     9061 tgcataaaaa acagactaca taatactgta aaacacaaca tatccagtca ctatggcggc
     9121 cgcattaggc accccaggct ttacacttta tgcttccggc tcgtataatg tgtggatttt
     9181 gagttaggat ccgtcgagat tttcaggagc taaggaagct aaaatggaga aaaaaatcac
     9241 tggatatacc accgttgata tatcccaatg gcatcgtaaa gaacattttg aggcatttca
     9301 gtcagttgct caatgtacct ataaccagac cgttcagctg gatattacgg cctttttaaa
     9361 gaccgtaaag aaaaataagc acaagtttta tccggccttt attcacattc ttgcccgcct
     9421 gatgaatgct catccggaat tccgtatggc aatgaaagac ggtgagctgg tgatatggga
     9481 tagtgttcac ccttgttaca ccgttttcca tgagcaaact gaaacgtttt catcgctctg
     9541 gagtgaatac cacgacgatt tccggcagtt tctacacata tattcgcaag atgtggcgtg
     9601 ttacggtgaa aacctggcct atttccctaa agggtttatt gagaatatgt ttttcgtctc
     9661 agccaatccc tgggtgagtt tcaccagttt tgatttaaac gtggccaata tggacaactt
     9721 cttcgccccc gttttcacca tgggcaaata ttatacgcaa ggcgacaagg tgctgatgcc
     9781 gctggcgatt caggttcatc atgccgtttg tgatggcttc catgtcggca gaatgcttaa
     9841 tgaattacaa cagtactgcg atgagtggca gggcggggcg taaacgcgtg gatccggctt
     9901 actaaaagcc agataacagt atgcgtattt gcgcgctgat ttttgcggta taagaatata
     9961 tactgatatg tatacccgaa gtatgtcaaa aagaggtatg ctatgaagca gcgtattaca
    10021 gtgacagttg acagcgacag ctatcagttg ctcaaggcat atatgatgtc aatatctccg
    10081 gtctggtaag cacaaccatg cagaatgaag cccgtcgtct gcgtgccgaa cgctggaaag
    10141 cggaaaatca ggaagggatg gctgaggtcg cccggtttat tgaaatgaac ggctcttttg
    10201 ctgacgagaa caggggctgg tgaaatgcag tttaaggttt acacctataa aagagagagc
    10261 cgttatcgtc tgtttgtgga tgtacagagt gatattattg acacgcccgg gcgacggatg
    10321 gtgatccccc tggccagtgc acgtctgctg tcagataaag tctcccgtga actttacccg
    10381 gtggtgcata tcggggatga aagctggcgc atgatgacca ccgatatggc cagtgtgccg
    10441 gtctccgtta tcggggaaga agtggctgat ctcagccacc gcgaaaatga catcaaaaac
    10501 gccattaacc tgatgttctg gggaatataa atgtcaggct cccttataca cagccagtct
    10561 gcaggtcgac
//
