LOCUS       sequence-176743-       10375 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses BE3-NLS with an N-terminal His Tag (His6) for bacterial 
            expression.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10375)
  AUTHORS   Rees HA, Komor AC, Yeh WH, Caetano-Lopes J, Warman M, Edge ASB, Liu 
            DR
  TITLE     Improving the DNA specificity and applicability of base editing 
            through protein engineering and protein delivery.
  JOURNAL   Nat Commun. 2017 Jun 6;8:15790. doi: 10.1038/ncomms15790.
  PUBMED    28585549
REFERENCE   2  (bases 1 to 10375)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2017-06-6"; volume: "8:15790. doi"; pages: " 
            10.1038/ncomms15790"
COMMENT     Sequence Label: pET42b-BE3
FEATURES             Location/Qualifiers
     source          1..10375
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             21..38
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             39..722
                     /codon_start=1
                     /product="cytidine deaminase (C to U editing enzyme) from 
                     rat"
                     /label=APOBEC-1
                     /note="can use ssDNA as a substrate (Komor et al., 2016)"
                     /translation="SSETGPVAVDPTLRRRIEPHEFEVFFDPRELRKETCLLYEINWGG
                     RHSIWRHTSQNTNKHVEVNFIEKFTTERYFCPNTRCSITWFLSWSPCGECSRAITEFLS
                     RYPHVTLFIYIARLYHHADPRNRQGLRDLISSGVTIQIMTEQESGYCWRNFVNYSPSNE
                     AHWPRYPHLWVRLYVLELYCIILGLPPCLNILRRKQPQLTFFTIALQSCHYQRLPPHIL
                     WATGLK"
     CDS             join(771..794,795..797,798..4871)
                     /codon_start=1
                     /product="nickase mutant of the Cas9 endonuclease from the 
                     Streptococcus pyogenes Type II CRISPR/Cas system"
                     /label=Cas9(D10A)
                     /note="generates RNA-guided single strand nicks in DNA due 
                     to the D10A mutation in the RuvC catalytic domain"
                     /translation="DKKYSIGLAIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKKN
                     LIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEESF
                     LVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIKF
                     RGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRLE
                     NLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQI
                     GDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVRQ
                     QLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLLR
                     KQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARGN
                     SRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEYF
                     TVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFDS
                     VEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEERL
                     KTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFMQ
                     LIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGRH
                     KPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLYL
                     YYLQNGRDMYVDQELDINRLSDYDVDHIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVPS
                     EEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKHV
                     AQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYLN
                     AVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKTE
                     ITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSKE
                     SILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGIT
                     IMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNEL
                     ALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILADA
                     NLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVLD
                     ATLIHQSITGLYETRIDLSQLGGD"
     CDS             4884..5132
                     /codon_start=1
                     /product="uracil-DNA glycosylase inhibitor from a Bacillus 
                     subtilis bacteriophage (Mol et al., 1995)"
                     /label=UGI
                     /translation="TNLSDIIEKETGKQLVIQESILMLPEEVEEVIGNKPESDILVHTA
                     YDESTDENVMLLTSDAPEYKPWALVIQDSNGENKIKML"
     CDS             5145..5165
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     primer_bind     complement(5231..5249)
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
     terminator      5245..5292
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     rep_origin      5329..5784
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(5416..5435)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     5626..5647
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     CDS             complement(5876..6691)
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     CDS             complement(5876..6691)
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     6599..6618
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     primer_bind     6683..6702
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     rep_origin      6813..7401
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     7302..7321
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     7555..7572
                     /label=L4440
                     /note="L4440 vector, forward primer"
     misc_feature    7587..7726
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     complement(7652..7671)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     7812..7834
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     CDS             complement(7828..8019)
                     /codon_start=1
                     /gene="rop"
                     /product="Rop protein, which maintains plasmids at low copy
                     number"
                     /label=rop
                     /translation="MTKQEKTALNMARFIRSQTLTLLEKLNELDADEQADICESLHDHA
                     YELYRSCLARFGDDGENL"
     CDS             complement(8828..9910)
                     /codon_start=1
                     /gene="lacI"
                     /product="lac repressor"
                     /label=lacI
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
                     /translation="MKPVTLYDVAEYAGVSYQTVSRVVNHASHVSAKTREKVEAAMAEL
                     NYIPNRVAQQLAGKQLLLIGVATSSLALHAPSQIVAAIKSRADQLGASVVVSMVERSGV
                     EACKAAVHNLLAQRVSGLIINYPLDDQDAIAVEAACTNVPALFLDVSDQTPINSIIFSH
                     EDGTRLGVEHLVALGHQQIALLAGPLSSVSARLRLAGWHKYLTRNQIQPIAEREGDWSA
                     MSGFQQTMQMLNEGIVPTAMLVANDQMALGAMRAITESGLRVGADISVVGYDDTEDSSC
                     YIPPLTTIKQDFRLLGQTSVDRLLQLSQGQAVKGNQLLPVSLVKRKTTLAPNTQTASPR
                     ALADSLMQLARQVSRLESWQ"
     primer_bind     9872..9891
                     /label=LacI-R
                     /note="LacI, reverse primer"
     promoter        complement(9911..9988)
                     /gene="lacI"
                     /label=lacI promoter
     primer_bind     10194..10213
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
     primer_bind     10297..10316
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        10297..10315
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    10316..10340
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     RBS             join(10355..10375,1..2)
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
ORIGIN
        1 gatataccat gggcagcagt catcatcatc accatcactc ttctgaaacc ggtccggttg
       61 cggttgaccc gaccctgcgt cgtcgtatcg aaccgcacga attcgaagtt ttcttcgacc
      121 cgcgtgaact gcgtaaagaa acctgcctgc tgtacgaaat caactggggt ggtcgtcact
      181 ctatctggcg tcacacctct cagaacacca acaaacacgt tgaagttaac ttcatcgaaa
      241 aattcaccac cgaacgttac ttctgcccga acacccgttg ctctatcacc tggttcctgt
      301 cttggtctcc gtgcggtgaa tgctctcgtg cgatcaccga attcctgtct cgttacccgc
      361 acgttaccct gttcatctac atcgcgcgtc tgtaccacca cgcggacccg cgtaaccgtc
      421 agggtctgcg tgacctgatc tcttctggtg ttaccatcca gatcatgacc gaacaggaat
      481 ctggttactg ctggcgtaac ttcgttaact actctccgtc taacgaagcg cactggccgc
      541 gttacccgca cctgtgggtt cgtctgtacg ttctggaact gtactgcatc atcctgggtc
      601 tgccgccgtg cctgaacatc ctgcgtcgta aacagccgca gctgaccttc ttcaccatcg
      661 cgctgcagtc ttgccactac cagcgtctgc cgccgcacat cctgtgggcg accggtctga
      721 aatccggtag cgaaacaccg gggacttcag aatcggccac cccggagtct gataagaaat
      781 actcaatagg cttagctatc ggcacaaata gcgtcggatg ggcggtgatc actgatgaat
      841 ataaggttcc gtctaaaaag ttcaaggttc tgggaaatac agaccgccac agtatcaaaa
      901 aaaatcttat aggggctctt ttatttgaca gtggagagac agcggaagcg actcgtctca
      961 aacggacagc tcgtagaagg tatacacgtc ggaagaatcg tatttgttat ctacaggaga
     1021 ttttttcaaa tgagatggcg aaagtagatg atagtttctt tcatcgactt gaagagtctt
     1081 ttttggtgga agaagacaag aagcatgaac gtcatcctat ttttggaaat atagtagatg
     1141 aagttgctta tcatgagaaa tatccaacta tctatcatct gcgaaaaaaa ttggtagatt
     1201 ctactgataa agcggatttg cgcttaatct atttggcctt agcgcatatg attaagtttc
     1261 gtggtcattt tttgattgag ggagatttaa atcctgataa tagtgatgtg gacaaactat
     1321 ttatccagtt ggtacaaacc tacaatcaat tatttgaaga aaaccctatt aacgcaagtg
     1381 gagtagatgc taaagcgatt ctttctgcac gattgagtaa atcaagacga ttagaaaatc
     1441 tcattgctca gctccccggt gagaagaaaa atggcttatt tgggaatctc attgctttgt
     1501 cattgggttt gacccctaat tttaaatcaa attttgattt ggcagaagat gctaaattac
     1561 agctttcaaa agatacttac gatgatgatt tagataattt attggcgcaa attggagatc
     1621 aatatgctga tttgtttttg gcagctaaga atttatcaga tgctatttta ctttcagata
     1681 tcctaagagt aaatactgaa ataactaagg ctcccctatc agcttcaatg attaaacgct
     1741 acgatgaaca tcatcaagac ttgactcttt taaaagcttt agttcgacaa caacttccag
     1801 aaaagtataa agaaatcttt tttgatcaat caaaaaacgg atatgcaggt tatattgatg
     1861 ggggagctag ccaagaagaa ttttataaat ttatcaaacc aattttagaa aaaatggatg
     1921 gtactgagga attattggtg aaactaaatc gtgaagattt gctgcgcaag caacggacct
     1981 ttgacaacgg ctctattccc catcaaattc acttgggtga gctgcatgct attttgagaa
     2041 gacaagaaga cttttatcca tttttaaaag acaatcgtga gaagattgaa aaaatcttga
     2101 cttttcgaat tccttattat gttggtccat tggcgcgtgg caatagtcgt tttgcatgga
     2161 tgactcggaa gtctgaagaa acaattaccc catggaattt tgaagaagtt gtcgataaag
     2221 gtgcttcagc tcaatcattt attgaacgca tgacaaactt tgataaaaat cttccaaatg
     2281 aaaaagtact accaaaacat agtttgcttt atgagtattt tacggtttat aacgaattga
     2341 caaaggtcaa atatgttact gaaggaatgc gaaaaccagc atttctttca ggtgaacaga
     2401 agaaagccat tgttgattta ctcttcaaaa caaatcgaaa agtaaccgtt aagcaattaa
     2461 aagaagatta tttcaaaaaa atagaatgtt ttgatagtgt tgaaatttca ggagttgaag
     2521 atagatttaa tgcttcatta ggtacctacc atgatttgct aaaaattatt aaagataaag
     2581 attttttgga taatgaagaa aatgaagata tcttagagga tattgtttta acattgacct
     2641 tatttgaaga tagggagatg attgaggaaa gacttaaaac atatgctcac ctctttgatg
     2701 ataaggtgat gaaacagctt aaacgtcgcc gttatactgg ttggggacgt ttgtctcgaa
     2761 aattgattaa tggtattagg gataagcaat ctggcaaaac aatattagat tttttgaaat
     2821 cagatggttt tgccaatcgc aattttatgc agctgatcca tgatgatagt ttgacattta
     2881 aagaagacat tcaaaaagca caagtgtctg gacaaggcga tagtttacat gaacatattg
     2941 caaatttagc tggtagccct gctattaaaa aaggtatttt acagactgta aaagttgttg
     3001 atgaattggt caaagtaatg gggcggcata agccagaaaa tatcgttatt gaaatggcac
     3061 gtgaaaatca gacaactcaa aagggccaga aaaattcgcg agagcgtatg aaacgaatcg
     3121 aagaaggtat caaagaatta ggaagtcaga ttcttaaaga gcatcctgtt gaaaatactc
     3181 aattgcaaaa tgaaaagctc tatctctatt atctccaaaa tggaagagac atgtatgtgg
     3241 accaagaatt agatattaat cgtttaagtg attatgatgt cgatcacatt gttccacaaa
     3301 gtttccttaa agacgattca atagacaata aggtcttaac gcgttctgat aaaaatcgtg
     3361 gtaaatcgga taacgttcca agtgaagaag tagtcaaaaa gatgaaaaac tattggagac
     3421 aacttctaaa cgccaagtta atcactcaac gtaagtttga taatttaacg aaagctgaac
     3481 gtggaggttt gagtgaactt gataaagctg gttttatcaa acgccaattg gttgaaactc
     3541 gccaaatcac taagcatgtg gcacaaattt tggatagtcg catgaatact aaatacgatg
     3601 aaaatgataa acttattcga gaggttaaag tgattacctt aaaatctaaa ttagtttctg
     3661 acttccgaaa agatttccaa ttctataaag tacgtgagat taacaattac catcatgccc
     3721 atgatgcgta tctaaatgcc gtcgttggaa ctgctttgat taagaaatat ccaaaacttg
     3781 aatcggagtt tgtctatggt gattataaag tttatgatgt tcgtaaaatg attgctaagt
     3841 ctgagcaaga aataggcaaa gcaaccgcaa aatatttctt ttactctaat atcatgaact
     3901 tcttcaaaac agaaattaca cttgcaaatg gagagattcg caaacgccct ctaatcgaaa
     3961 ctaatgggga aactggagaa attgtctggg ataaagggcg agattttgcc acagtgcgca
     4021 aagtattgtc catgccccaa gtcaatattg tcaagaaaac agaagtacag acaggcggat
     4081 tctccaagga gtcaatttta ccaaaaagaa attcggacaa gcttattgct cgtaaaaaag
     4141 actgggatcc aaaaaaatat ggtggttttg atagtccaac ggtagcttat tcagtcctag
     4201 ttgttgctaa ggtggaaaaa gggaaatcga agaagttaaa atccgttaaa gagttactag
     4261 ggatcacaat tatggaaaga agttcctttg aaaaaaatcc gattgacttt ttagaagcta
     4321 aaggatataa ggaagttaaa aaagacttaa tcattaaact acctaaatat agtctttttg
     4381 agttagaaaa cggtcgtaaa cggatgctgg ctagtgccgg agaattacaa aaaggaaatg
     4441 agctggctct gccaagcaaa tatgtgaatt ttttatattt agctagtcat tatgaaaagt
     4501 tgaagggtag tccagaagat aacgaacaaa aacaattgtt tgtggagcag cataagcatt
     4561 atttagatga gattattgag caaatcagtg aattttctaa gcgtgttatt ttagcagatg
     4621 ccaatttaga taaagttctt agtgcatata acaaacatag agacaaacca atacgtgaac
     4681 aagcagaaaa tattattcat ttatttacgt tgacgaatct tggagctccc gctgctttta
     4741 aatattttga tacaacaatt gatcgtaaac gatatacgtc tacaaaagaa gttttagatg
     4801 ccactcttat ccatcaatcc atcactggtc tttatgaaac acgcattgat ttgagtcagc
     4861 taggaggtga ctctggtggt tctactaatc tgtcagatat tattgaaaag gagaccggta
     4921 agcaactggt tatccaggaa tccatcctca tgctcccaga ggaggtggaa gaagtcattg
     4981 ggaacaagcc ggaaagcgat atactcgtgc acaccgccta cgacgagagc accgacgaga
     5041 atgtcatgct tctgactagc gacgcccctg aatacaagcc ttgggctctg gtcatacagg
     5101 atagcaacgg tgagaacaag attaagatgc tctctggtgg ttctcccaag aagaagagga
     5161 aagtctaata attgattaat acctaggctg ctaaacaaag cccgaaagga agctgagttg
     5221 gctgctgcca ccgctgagca ataactagca taaccccttg gggcctctaa acgggtcttg
     5281 aggggttttt tgctgaaagg aggaactata tccggattgg cgaatgggac gcgccctgta
     5341 gcggcgcatt aagcgcggcg ggtgtggtgg ttacgcgcag cgtgaccgct acacttgcca
     5401 gcgccctagc gcccgctcct ttcgctttct tcccttcctt tctcgccacg ttcgccggct
     5461 ttccccgtca agctctaaat cgggggctcc ctttagggtt ccgatttagt gctttacggc
     5521 acctcgaccc caaaaaactt gattagggtg atggttcacg tagtgggcca tcgccctgat
     5581 agacggtttt tcgccctttg acgttggagt ccacgttctt taatagtgga ctcttgttcc
     5641 aaactggaac aacactcaac catatctcgg tctattcttt tgatttataa gggattttgc
     5701 cgatttcggc ctattggtta aaaaatgagc tgatttaaca aaaatttaac gcgaatttta
     5761 acaaaatatt aacgcttaca atttaggtgg cacttttcgg ggaaatgtgc gcggaacccc
     5821 tatttgttta tttttctaaa tacattcaaa tatgtatccg ctcatgaatt aattcttaga
     5881 aaaactcatc gagcatcaaa tgaaactgca atttattcat atcaggatta tcaataccat
     5941 atttttgaaa aagccgtttc tgtaatgaag gagaaaactc accgaggcag ttccatagga
     6001 tggcaagatc ctggtatcgg tctgcgattc cgactcgtcc aacatcaata caacctatta
     6061 atttcccctc gtcaaaaata aggttatcaa gtgagaaatc accatgagtg acgactgaat
     6121 ccggtgagaa tggcaaaagt ttatgcattt ctttccagac ttgttcaaca ggccagccat
     6181 tacgctcgtc atcaaaatca ctcgcatcaa ccaaaccgtt attcattcgt gattgcgcct
     6241 gagcgagacg aaatacgcga tcgctgttaa aaggacaatt acaaacagga atcgaatgca
     6301 accggcgcag gaacactgcc agcgcatcaa caatattttc acctgaatca ggatattctt
     6361 ctaatacctg gaatgctgtt ttcccgggga tcgcagtggt gagtaaccat gcatcatcag
     6421 gagtacggat aaaatgcttg atggtcggaa gaggcataaa ttccgtcagc cagtttagtc
     6481 tgaccatctc atctgtaaca tcattggcaa cgctaccttt gccatgtttc agaaacaact
     6541 ctggcgcatc gggcttccca tacaatcgat agattgtcgc acctgattgc ccgacattat
     6601 cgcgagccca tttataccca tataaatcag catccatgtt ggaatttaat cgcggcctag
     6661 agcaagacgt ttcccgttga atatggctca taacacccct tgtattactg tttatgtaag
     6721 cagacagttt tattgttcat gaccaaaatc ccttaacgtg agttttcgtt ccactgagcg
     6781 tcagaccccg tagaaaagat caaaggatct tcttgagatc ctttttttct gcgcgtaatc
     6841 tgctgattgc aaacaaaaaa accaccgcta ccagcggtgg tttgtttgcc ggatcaagag
     6901 ctaccaactc tttttccgaa ggtaactggc ttcagcagag cgcagatacc aaatactgtc
     6961 cttctagtgt agccgtagtt aggccaccac ttcaagaact ctgtagcacc gcctacatac
     7021 ctcgctctgc taatcatgtt accagtggct gctgccagtg gcgataagtc gtgtcttacc
     7081 gggttggact caagacgata gttaccggat aaggcgcagc ggtcgggctg aacggggggt
     7141 tcgtgcacac agccaagctt ggagcgaacg acctacaccg aactgagata cctacagcgt
     7201 gagctatgag aaagcgccac gcttcccgaa gggagaaagg cggacaggta tccggtaagc
     7261 ggcagggtcg gaacaggaga gcgcacgagg gagcttccag ggggaaacgc ctggtatctt
     7321 tatagtcctg tcgggtttcg ccacctctga cttgagcgtc gatttttgtg atgctcgtca
     7381 ggggggcgga gcctatggaa aaacgccagc aacgcggcct ttttacggtt cctggccttt
     7441 tgctggcctt ttgctcacat gttctttcct gcgttatccc ctgattctgt ggataaccgt
     7501 attaccgcct ttgagtgagc tgataccgct cgccgcagcc gaacgaccga gcgcagcgag
     7561 tcagtgagcg aggaagcgga agagcgcctg atgcggtatt ttctccttac gcatctgtgc
     7621 ggtatttcac accgcaatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa
     7681 gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc
     7741 aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc
     7801 tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc
     7861 gaggcagctg cggtaaagct cataagcgtg gtcgtgaagc gattcacaga tgtctgcctg
     7921 ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa
     7981 gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg
     8041 atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt
     8101 tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg
     8161 gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga
     8221 tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt
     8281 gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca
     8341 tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat
     8401 cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga
     8461 caggagcacg atcatgcgca cccgtggggc cgccatgccg gcgataatgg cctgcttctc
     8521 gccgaaacgt ttggtggcgg gaccagtgac gaaggcttga gcgagggagt gcaagattcc
     8581 gaataccgca agcgacaggc cgatcatcgt cgcgctccag cgaaagcggt cctcgccgaa
     8641 aatgacccag agcgctgccg gcacctgtcc tacgagttgc atgataaaga agacagtcat
     8701 aagtgcggcg acgatagtca tgccccgcgc ccaccggaag gagctgactg ggttgaaggc
     8761 tctcaagggc atcggtcgag atcccggtgc ctaatgagtg agctaactta cattaattgc
     8821 gttgcgctca ctgccagctt tccagtcggg aaacctgtcg tgccagctgc attaatgaat
     8881 cggccaacgc gcggggagag gcggtttgcg tattgggcgc cagggtggtt tttcttttca
     8941 ccagtgagac gggcaacagc tgattgccct tcaccgcctg gccctgagag agttgcagca
     9001 agcggtccac gctggtttgc cccagcaggc gaaaatcctg tttgatggtg gttaacggcg
     9061 ggatataaca tgagctgtct tcggtatcgt cgtatcccac taccgagata tccgcaccaa
     9121 cgcgcagccc ggactcggta atggcgcgca ttgcgcccag cgccatctga tcgttggcaa
     9181 ccagcatcgc agtgggaacg atgccctcat tcagcatttg catggtttgt tgaaaaccgg
     9241 acatggcact ccagtcgcct tcccgttccg ctatcggctg aatttgattg cgagtgagat
     9301 atttatgcca gccagccaga cgcagacgcg ccgagacaga acttaatggg cccgctaaca
     9361 gcgcgatttg ctggtgaccc aatgcgacca gatgctccac gcccagtcgc gtaccgtctt
     9421 catgggagaa aataatactg ttgatgggtg tctggtcaga gacatcaaga aataacgccg
     9481 gaacattagt gcaggcagct tccacagcaa tggcatcctg gtcatccagc ggatagttaa
     9541 tgatcagccc actgacgcgt tgcgcgagaa gattgtgcac cgccgcttta caggcttcga
     9601 cgccgcttcg ttctaccatc gacaccacca cgctggcacc cagttgatcg gcgcgagatt
     9661 taatcgccgc gacaatttgc gacggcgcgt gcagggccag actggaggtg gcaacgccaa
     9721 tcagcaacaa ctgtttgccc gccagttgtt gtgccacgcg gttgggaatg taattcagct
     9781 ccgccatcgc cgcttccact ttttcccgcg ttttcgcaga aacgtggctg gcatggttca
     9841 ccacgcggga aacggtctga taagagacac cggcatactc tgcgacatcg tataacgtta
     9901 ctggtttcac attcaccacc ctgaattgac tctcttccgg gcgctatcat gccataccgc
     9961 gaaaggtttt gcgccattcg atggtgtccg ggatctcgac gctctccctt atgcgactcc
    10021 tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat
    10081 ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc
    10141 acgccgaaac aagcgctcat gagcccaaag tggcgagccc gatcttcccc atcggtgatg
    10201 tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt
    10261 ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact ataggggaat
    10321 tgtgagcgga taacaattcc cctctagaaa taattttgtt taactttaag aagga
//
