LOCUS       sequence-176791-        4330 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4330)
  AUTHORS   Lim LP, Lau NC, Garrett-Engele P, Grimson A, Schelter JM, Castle J, 
            Bartel DP, Linsley PS, Johnson JM
  TITLE     Microarray analysis shows that some microRNAs downregulate large 
            numbers of target mRNAs.
  JOURNAL   Nature. 2005 Feb 17. 433(7027):769-73.
  PUBMED    15685193
REFERENCE   2  (bases 1 to 4330)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nature. 
            2005 Feb 17. 433(7027):769-73."
COMMENT     Sequence Label: pIS54 ADAR 3'UTR mut
FEATURES             Location/Qualifiers
     source          1..4330
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        1..19
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             29..964
                     /codon_start=1
                     /product="luciferase from the anthozoan coelenterate 
                     Renilla reniformis (sea pansy)"
                     /label=Rluc
                     /translation="MTSKVYDPEQRKRMITGPQWWARCKQMNVLDSFINYYDSEKHAEN
                     AVIFLHGNAASSYLWRHVVPHIEPVARCIIPDLIGMGKSGKSGNGSYRLLDHYKYLTAW
                     FELLNLPKKIIFVGHDWGACLAFHYSYEHQDKIKAIVHAESVVDVIESWDEWPDIEEDI
                     ALIKSEEGEKMVLENNFFVETMLPSKIMRKLEPEEFAAYLEPFKEKGEVRRPTLSWPRE
                     IPLVKGGKPDVVQIVRNYNAYLRASDDLPKMFIESDPGFFSNAIVEGAKKFPNTEFVKV
                     KGLHFSQEDAPDEMGKYIKSFVERVLKNEQ"
     polyA_signal    1280..1401
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(1291..1310)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     1345..1364
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     promoter        1534..1638
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(1639..1707,1708..2499)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(1857..1876)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      2670..3258
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     3159..3178
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     promoter        3333..4084
                     /label=HSV TK promoter
                     /note="herpes simplex virus thymidine kinase promoter"
     intron          4154..4286
                     /label=chimeric intron
                     /note="chimera between introns from human beta-globin and 
                     immunoglobulin heavy chain genes"
     primer_bind     4269..4288
                     /label=pMT2-F
                     /note="Synthetic intron, forward primer"
ORIGIN
        1 taatacgact cactataggc tagccaccat gacttcgaaa gtttatgatc cagaacaaag
       61 gaaacggatg ataactggtc cgcagtggtg ggccagatgt aaacaaatga atgttcttga
      121 ttcatttatt aattattatg attcagaaaa acatgcagaa aatgctgtta tttttttaca
      181 tggtaacgcg gcctcttctt atttatggcg acatgttgtg ccacatattg agccagtagc
      241 gcggtgtatt ataccagacc ttattggtat gggcaaatca ggcaaatctg gtaatggttc
      301 ttataggtta cttgatcatt acaaatatct tactgcatgg tttgaacttc ttaatttacc
      361 aaagaagatc atttttgtcg gccatgattg gggtgcttgt ttggcatttc attatagcta
      421 tgagcatcaa gataagatca aagcaatagt tcacgctgaa agtgtagtag atgtgattga
      481 atcatgggat gaatggcctg atattgaaga agatattgcg ttgatcaaat ctgaagaagg
      541 agaaaaaatg gttttggaga ataacttctt cgtggaaacc atgttgccat caaaaatcat
      601 gagaaagtta gaaccagaag aatttgcagc atatcttgaa ccattcaaag agaaaggtga
      661 agttcgtcgt ccaacattat catggcctcg tgaaatcccg ttagtaaaag gtggtaaacc
      721 tgacgttgta caaattgtta ggaattataa tgcttatcta cgtgcaagtg atgatttacc
      781 aaaaatgttt attgaatcgg acccaggatt cttttccaat gctattgttg aaggtgccaa
      841 gaagtttcct aatactgaat ttgtcaaagt aaaaggtctt catttttcgc aagaagatgc
      901 acctgatgaa atgggaaaat atatcaaatc gttcgttgag cgagttctca aaaatgaaca
      961 ataattctag gagctcttga tttgtctgaa ctgtggctac tgtgaagtac ttgaataatc
     1021 acttgtaaaa attgtcagtg cttgaagctg tttcctttac tcacattgaa gggacttcgt
     1081 tggttttttg gagtcttggt tgtgactcca agagcagagt gaggaagacc cccaagcata
     1141 gactcgggta ctgtgatgat ggctgcagtc cagttttatg attctgcttt tatgtgtccc
     1201 ttgataacag tgacttaaca atataaagta ctcataaata aatctagcgt tctagagcgg
     1261 ccgcttcgag cagacatgat aagatacatt gatgagtttg gacaaaccac aactagaatg
     1321 cagtgaaaaa aatgctttat ttgtgaaatt tgtgatgcta ttgctttatt tgtaaccatt
     1381 ataagctgca ataaacaagt taacaacaac aattgcattc attttatgtt tcaggttcag
     1441 ggggaggtgt gggaggtttt ttaaagcaag taaaacctct acaaatgtgg taaaatcgat
     1501 aaggatccag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc
     1561 taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa
     1621 tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt
     1681 gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct
     1741 gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc
     1801 cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta
     1861 tgtggcgcgg tattatcccg tattgacgcc gggcaagagc aactcggtcg ccgcatacac
     1921 tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc
     1981 atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac
     2041 ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg
     2101 gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac
     2161 gagcgtgaca ccacgatgcc tgtagcaatg gcaacaacgt tgcgcaaact attaactggc
     2221 gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt
     2281 gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga
     2341 gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc
     2401 cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag
     2461 atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca
     2521 tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc
     2581 ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca
     2641 gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc
     2701 tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta
     2761 ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgttctt
     2821 ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc
     2881 gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg
     2941 ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg
     3001 tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag
     3061 ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc
     3121 agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat
     3181 agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg
     3241 gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc
     3301 tggccttttg ctcacatggc tcgacagatc taaatgagtc ttcggacctc gcgggggccg
     3361 cttaagcggt ggttagggtt tgtctgacgc ggggggaggg ggaaggaacg aaacactctc
     3421 attcggaggc ggctcggggt ttggtcttgg tggccacggg cacgcagaag agcgccgcga
     3481 tcctcttaag cacccccccg ccctccgtgg aggcgggggt ttggtcggcg ggtggtaact
     3541 ggcgggccgc tgactcgggc gggtcgcgcg ccccagagtg tgaccttttc ggtctgctcg
     3601 cagacccccg ggcggcgccg ccgcggcggc gacgggctcg ctgggtccta ggctccatgg
     3661 ggaccgtata cgtggacagg ctctggagca tccgcacgac tgcggtgata ttaccggaga
     3721 ccttctgcgg gacgagccgg gtcacgcggc tgacgcggag cgtccgttgg gcgacaaaca
     3781 ccaggacggg gcacaggtac actatcttgt cacccggagg cgcgagggac tgcaggagct
     3841 tcagggagtg gcgcagctgc ttcatccccg tggcccgttg ctcgcgtttg ctggcggtgt
     3901 ccccggaaga aatatatttg catgtcttta gttctatgat gacacaaacc ccgcccagcg
     3961 tcttgtcatt ggcgaattcg aacacgcaga tgcagtcggg gcggcgcggt cccaggtcca
     4021 cttcgcatat taaggtgacg cgtgtggcct cgaacaccga gcgaccctgc agcgacccgc
     4081 ttaaaagctt gattcttctg acacaacagt ctcgaactta agctgcagaa gttggtcgtg
     4141 aggcactggg caggtaagta tcaaggttac aagacaggtt taaggagacc aatagaaact
     4201 gggcttgtcg agacagagaa gactcttgcg tttctgatag gcacctattg gtcttactga
     4261 catccacttt gcctttctct ccacaggtgt ccactcccag ttcaattaca gctcttaagg
     4321 ctagagtact
//
