LOCUS       sequence-176793-        3440 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  bacterial expression of moxDendra2.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3440)
  AUTHORS   Kaberniuk AA, Morano NC, Verkhusha VV, Snapp EL
  TITLE     moxDendra2: an inert photoswitchable protein for oxidizing 
            environments.
  JOURNAL   Chem Commun (Camb). 2017 Feb 9;53(13):2106-2109. doi: 
            10.1039/c6cc09997a.
  PUBMED    28133646
REFERENCE   2  (bases 1 to 3440)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10"; journalName: 
            "Chem Commun (Camb)"; date: "2017-02-9- 9"; volume: "53"; issue: 
            "13"; pages: "2106-2109"
COMMENT     Sequence Label: pUC57 moxDendra2
FEATURES             Location/Qualifiers
     source          1..3440
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    1..22
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(37..42,43..60,61..67)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    75..91
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     80..102
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     99..115
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     99..115
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     CDS             194..886
                     /codon_start=1
                     /product="monomeric photoswitchable green-to-red 
                     fluorescent protein from Dendronephthya"
                     /label=Dendra2
                     /note="mammalian codon-optimized"
                     /translation="MNTPGINLIKEDMRVKVHMEGNVNGHAFVIEGEGKGKPYEGTQTA
                     QLTVKEGAPLPFSYDILTTAVHYGNRVFTKYPEDIPDYFKQSFPEGYSWERTMTFEDKG
                     IATIRSDISLEGDTFFQNVRFKGTNFPPNGPVMQKKTLKWEPSTEKLHVRDGLLVGNIN
                     MALLLEGGGHYLADFKTTYKAKKVVQLPDAHFVDHRIEILGQDSDYNKVKLYEHAVARY
                     SPLPSQVW"
     primer_bind     complement(939..956)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(939..955)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(948..970)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     complement(1164..1183)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     1283..1305
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(1343..1361)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        1429..1533
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(1534..1602,1603..2394)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(1752..1771)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      2565..3153
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     3054..3073
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     3307..3324
                     /label=L4440
                     /note="L4440 vector, forward primer"
ORIGIN
        1 taatgtgagt tagctcactc attaggcacc ccaggcttta cactttatgc ttccggctcg
       61 tatgttgtgt ggaattgtga gcggataaca atttcacaca ggaaacagct atgaccatga
      121 ttacgccaag cttgcatgca ggcctctgca gtcgacgggc ccgggatccg atgacccaat
      181 accggtcgcc accatgaaca ccccgggaat taacctgatc aaggaggaca tgcgcgtgaa
      241 ggtgcacatg gagggcaacg tgaacggcca cgccttcgtg atcgagggcg agggcaaggg
      301 caagccctac gagggcaccc agaccgccca gctgaccgtg aaggagggcg cccccctgcc
      361 cttcagctac gacatcctga ccaccgccgt gcactacggc aaccgggtgt tcaccaagta
      421 ccccgaggac atccccgact acttcaagca gagcttcccc gagggctaca gctgggagcg
      481 caccatgacc ttcgaggaca agggcatcgc caccatccgc agcgacatca gcctggaggg
      541 cgacaccttc ttccagaacg tgcgcttcaa gggcaccaac ttccccccca acggccccgt
      601 gatgcagaag aagaccctga agtgggagcc cagcaccgag aagctgcacg tgcgcgacgg
      661 cctgctggtg ggcaacatca acatggccct gctgctggag ggcggcggcc actacctggc
      721 cgacttcaag accacctaca aggccaagaa ggtggtgcag ctgcccgacg cccacttcgt
      781 ggaccaccgc atcgagatcc tgggccagga cagcgactac aacaaggtga agctgtacga
      841 gcacgccgtg gcccgctaca gccccctgcc cagccaggtg tggtaagcgg ccgcattggg
      901 tcatctagat gcattcgcga ggtaccgagc tcgaattcac tggccgtcgt tttacaacgt
      961 cgtgactggg aaaaccctgg cgttacccaa cttaatcgcc ttgcagcaca tccccctttc
     1021 gccagctggc gtaatagcga agaggcccgc accgatcgcc cttcccaaca gttgcgcagc
     1081 ctgaatggcg aatggcgcct gatgcggtat tttctcctta cgcatctgtg cggtatttca
     1141 caccgcatat ggtgcactct cagtacaatc tgctctgatg ccgcatagtt aagccagccc
     1201 cgacacccgc caacacccgc tgacgcgccc tgacgggctt gtctgctccc ggcatccgct
     1261 tacagacaag ctgtgaccgt ctccgggagc tgcatgtgtc agaggttttc accgtcatca
     1321 ccgaaacgcg cgagacgaaa gggcctcgtg atacgcctat ttttataggt taatgtcatg
     1381 ataataatgg tttcttagac gtcaggtggc acttttcggg gaaatgtgcg cggaacccct
     1441 atttgtttat ttttctaaat acattcaaat atgtatccgc tcatgagaca ataaccctga
     1501 taaatgcttc aataatattg aaaaaggaag agtatgagta ttcaacattt ccgtgtcgcc
     1561 cttattccct tttttgcggc attttgcctt cctgtttttg ctcacccaga aacgctggtg
     1621 aaagtaaaag atgctgaaga tcagttgggt gcacgagtgg gttacatcga actggatctc
     1681 aacagcggta agatccttga gagttttcgc cccgaagaac gttttccaat gatgagcact
     1741 tttaaagttc tgctatgtgg cgcggtatta tcccgtattg acgccgggca agagcaactc
     1801 ggtcgccgca tacactattc tcagaatgac ttggttgagt actcaccagt cacagaaaag
     1861 catcttacgg atggcatgac agtaagagaa ttatgcagtg ctgccataac catgagtgat
     1921 aacactgcgg ccaacttact tctgacaacg atcggaggac cgaaggagct aaccgctttt
     1981 ttgcacaaca tgggggatca tgtaactcgc cttgatcgtt gggaaccgga gctgaatgaa
     2041 gccataccaa acgacgagcg tgacaccacg atgcctgtag caatggcaac aacgttgcgc
     2101 aaactattaa ctggcgaact acttactcta gcttcccggc aacaattaat agactggatg
     2161 gaggcggata aagttgcagg accacttctg cgctcggccc ttccggctgg ctggtttatt
     2221 gctgataaat ctggagccgg tgagcgtggg tctcgcggta tcattgcagc actggggcca
     2281 gatggtaagc cctcccgtat cgtagttatc tacacgacgg ggagtcaggc aactatggat
     2341 gaacgaaata gacagatcgc tgagataggt gcctcactga ttaagcattg gtaactgtca
     2401 gaccaagttt actcatatat actttagatt gatttaaaac ttcattttta atttaaaagg
     2461 atctaggtga agatcctttt tgataatctc atgaccaaaa tcccttaacg tgagttttcg
     2521 ttccactgag cgtcagaccc cgtagaaaag atcaaaggat cttcttgaga tccttttttt
     2581 ctgcgcgtaa tctgctgctt gcaaacaaaa aaaccaccgc taccagcggt ggtttgtttg
     2641 ccggatcaag agctaccaac tctttttccg aaggtaactg gcttcagcag agcgcagata
     2701 ccaaatactg ttcttctagt gtagccgtag ttaggccacc acttcaagaa ctctgtagca
     2761 ccgcctacat acctcgctct gctaatcctg ttaccagtgg ctgctgccag tggcgataag
     2821 tcgtgtctta ccgggttgga ctcaagacga tagttaccgg ataaggcgca gcggtcgggc
     2881 tgaacggggg gttcgtgcac acagcccagc ttggagcgaa cgacctacac cgaactgaga
     2941 tacctacagc gtgagctatg agaaagcgcc acgcttcccg aagggagaaa ggcggacagg
     3001 tatccggtaa gcggcagggt cggaacagga gagcgcacga gggagcttcc agggggaaac
     3061 gcctggtatc tttatagtcc tgtcgggttt cgccacctct gacttgagcg tcgatttttg
     3121 tgatgctcgt caggggggcg gagcctatgg aaaaacgcca gcaacgcggc ctttttacgg
     3181 ttcctggcct tttgctggcc ttttgctcac atgttctttc ctgcgttatc ccctgattct
     3241 gtggataacc gtattaccgc ctttgagtga gctgataccg ctcgccgcag ccgaacgacc
     3301 gagcgcagcg agtcagtgag cgaggaagcg gaagagcgcc caatacgcaa accgcctctc
     3361 cccgcgcgtt ggccgattca ttaatgcagc tggcacgaca ggtttcccga ctggaaagcg
     3421 ggcagtgagc gcaacgcaat
//
