LOCUS       sequence-176794-        4876 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  mammalian expression of GC localized moxDendra2.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4876)
  AUTHORS   Kaberniuk AA, Morano NC, Verkhusha VV, Snapp EL
  TITLE     moxDendra2: an inert photoswitchable protein for oxidizing 
            environments.
  JOURNAL   Chem Commun (Camb). 2017 Feb 9;53(13):2106-2109. doi: 
            10.1039/c6cc09997a.
  PUBMED    28133646
REFERENCE   2  (bases 1 to 4876)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10"; journalName: 
            "Chem Commun (Camb)"; date: "2017-02-9- 9"; volume: "53"; issue: 
            "13"; pages: "2106-2109"
COMMENT     Sequence Label: GalT-moxDendra2
FEATURES             Location/Qualifiers
     source          1..4876
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        1..304
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        305..508
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     459..479
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     CDS             790..1482
                     /codon_start=1
                     /product="monomeric photoswitchable green-to-red 
                     fluorescent protein from Dendronephthya"
                     /label=Dendra2
                     /note="mammalian codon-optimized"
                     /translation="MNTPGINLIKEDMRVKVHMEGNVNGHAFVIEGEGKGKPYEGTQTA
                     QLTVKEGAPLPFSYDILTTAVHYGNRVFTKYPEDIPDYFKQSFPEGYSWERTMTFEDKG
                     IATIRSDISLEGDTFFQNVRFKGTNFPPNGPVMQKKTLKWEPSTEKLHVRDGLLVGNIN
                     MALLLEGGGHYLADFKTTYKAKKVVQLPDAHFVDHRIEILGQDSDYNKVKLYEHAVARY
                     SPLPSQVW"
     polyA_signal    1604..1725
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(1641..1660)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     1695..1714
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      complement(1732..2187)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(1869..1890)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     2081..2100
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     promoter        2214..2318
                     /gene="bla"
                     /label=AmpR promoter
     promoter        2320..2677
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     enhancer        2320..2511
                     /label=SV40 enhancer
                     /note="enhancer for the SV40 early promoter (Herr, 1993)"
     primer_bind     complement(2343..2363)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     rep_origin      2528..2663
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             2712..3506
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     CDS             2712..3506
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(2766..2785)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     3376..3395
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     primer_bind     complement(3694..3713)
                     /label=TK-pA-R
                     /note="Thymidine kinase polyA, reverse primer"
     polyA_signal    3738..3785
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase 
                     polyadenylation signal (Cole and Stacy, 1985)"
     rep_origin      4114..4702
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     4603..4622
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc cccgcccatt
       61 gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc attgacgtca
      121 atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt atcatatgcc
      181 aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt atgcccagta
      241 catgacctta tgggactttc ctacttggca gtacatctac gtattagtca tcgctattac
      301 catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg actcacgggg
      361 atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc aaaatcaacg
      421 ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg gtaggcgtgt
      481 acggtgggag gtctatataa gcagagctgg tttagtgaac cgtcagatcc gctagcgcta
      541 ccggactcag atctcgagct cgccaccatg aggcttcggg agccgctcct gagcggcagc
      601 gccgcgatgc caggcgcgtc cctacagcgg gcctgccgcc tgctcgtggc cgtctgcgct
      661 ctgcaccttg gcgtcaccct cgtttactac ctggctggcc gcgacctgag ccgcctgccc
      721 caactggtcg gagtctccac accgctgcag tcgacggtac cgcgggcccg ggatccaccg
      781 gtcgccacca tgaacacccc gggaattaac ctgatcaagg aggacatgcg cgtgaaggtg
      841 cacatggagg gcaacgtgaa cggccacgcc ttcgtgatcg agggcgaggg caagggcaag
      901 ccctacgagg gcacccagac cgcccagctg accgtgaagg agggcgcccc cctgcccttc
      961 agctacgaca tcctgaccac cgccgtgcac tacggcaacc gggtgttcac caagtacccc
     1021 gaggacatcc ccgactactt caagcagagc ttccccgagg gctacagctg ggagcgcacc
     1081 atgaccttcg aggacaaggg catcgccacc atccgcagcg acatcagcct ggagggcgac
     1141 accttcttcc agaacgtgcg cttcaagggc accaacttcc cccccaacgg ccccgtgatg
     1201 cagaagaaga ccctgaagtg ggagcccagc accgagaagc tgcacgtgcg cgacggcctg
     1261 ctggtgggca acatcaacat ggccctgctg ctggagggcg gcggccacta cctggccgac
     1321 ttcaagacca cctacaaggc caagaaggtg gtgcagctgc ccgacgccca cttcgtggac
     1381 caccgcatcg agatcctggg ccaggacagc gactacaaca aggtgaagct gtacgagcac
     1441 gccgtggccc gctacagccc cctgcccagc caggtgtggt aagcggccgc gactctagat
     1501 cataatcagc cataccacat ttgtagaggt tttacttgct ttaaaaaacc tcccacacct
     1561 ccccctgaac ctgaaacata aaatgaatgc aattgttgtt gttaacttgt ttattgcagc
     1621 ttataatggt tacaaataaa gcaatagcat cacaaatttc acaaataaag catttttttc
     1681 actgcattct agttgtggtt tgtccaaact catcaatgta tcttaaggcg taaattgtaa
     1741 gcgttaatat tttgttaaaa ttcgcgttaa atttttgtta aatcagctca ttttttaacc
     1801 aataggccga aatcggcaaa atcccttata aatcaaaaga atagaccgag atagggttga
     1861 gtgttgttcc agtttggaac aagagtccac tattaaagaa cgtggactcc aacgtcaaag
     1921 ggcgaaaaac cgtctatcag ggcgatggcc cactacgtga accatcaccc taatcaagtt
     1981 ttttggggtc gaggtgccgt aaagcactaa atcggaaccc taaagggagc ccccgattta
     2041 gagcttgacg gggaaagccg gcgaacgtgg cgagaaagga agggaagaaa gcgaaaggag
     2101 cgggcgctag ggcgctggca agtgtagcgg tcacgctgcg cgtaaccacc acacccgccg
     2161 cgcttaatgc gccgctacag ggcgcgtcag gtggcacttt tcggggaaat gtgcgcggaa
     2221 cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac
     2281 cctgataaat gcttcaataa tattgaaaaa ggaagagtcc tgaggcggaa agaaccagct
     2341 gtggaatgtg tgtcagttag ggtgtggaaa gtccccaggc tccccagcag gcagaagtat
     2401 gcaaagcatg catctcaatt agtcagcaac caggtgtgga aagtccccag gctccccagc
     2461 aggcagaagt atgcaaagca tgcatctcaa ttagtcagca accatagtcc cgcccctaac
     2521 tccgcccatc ccgcccctaa ctccgcccag ttccgcccat tctccgcccc atggctgact
     2581 aatttttttt atttatgcag aggccgaggc cgcctcggcc tctgagctat tccagaagta
     2641 gtgaggaggc ttttttggag gcctaggctt ttgcaaagat cgatcaagag acaggatgag
     2701 gatcgtttcg catgattgaa caagatggat tgcacgcagg ttctccggcc gcttgggtgg
     2761 agaggctatt cggctatgac tgggcacaac agacaatcgg ctgctctgat gccgccgtgt
     2821 tccggctgtc agcgcagggg cgcccggttc tttttgtcaa gaccgacctg tccggtgccc
     2881 tgaatgaact gcaagacgag gcagcgcggc tatcgtggct ggccacgacg ggcgttcctt
     2941 gcgcagctgt gctcgacgtt gtcactgaag cgggaaggga ctggctgcta ttgggcgaag
     3001 tgccggggca ggatctcctg tcatctcacc ttgctcctgc cgagaaagta tccatcatgg
     3061 ctgatgcaat gcggcggctg catacgcttg atccggctac ctgcccattc gaccaccaag
     3121 cgaaacatcg catcgagcga gcacgtactc ggatggaagc cggtcttgtc gatcaggatg
     3181 atctggacga agagcatcag gggctcgcgc cagccgaact gttcgccagg ctcaaggcga
     3241 gcatgcccga cggcgaggat ctcgtcgtga cccatggcga tgcctgcttg ccgaatatca
     3301 tggtggaaaa tggccgcttt tctggattca tcgactgtgg ccggctgggt gtggcggacc
     3361 gctatcagga catagcgttg gctacccgtg atattgctga agagcttggc ggcgaatggg
     3421 ctgaccgctt cctcgtgctt tacggtatcg ccgctcccga ttcgcagcgc atcgccttct
     3481 atcgccttct tgacgagttc ttctgagcgg gactctgggg ttcgaaatga ccgaccaagc
     3541 gacgcccaac ctgccatcac gagatttcga ttccaccgcc gccttctatg aaaggttggg
     3601 cttcggaatc gttttccggg acgccggctg gatgatcctc cagcgcgggg atctcatgct
     3661 ggagttcttc gcccacccta gggggaggct aactgaaaca cggaaggaga caataccgga
     3721 aggaacccgc gctatgacgg caataaaaag acagaataaa acgcacggtg ttgggtcgtt
     3781 tgttcataaa cgcggggttc ggtcccaggg ctggcactct gtcgataccc caccgagacc
     3841 ccattggggc caatacgccc gcgtttcttc cttttcccca ccccaccccc caagttcggg
     3901 tgaaggccca gggctcgcag ccaacgtcgg ggcggcaggc cctgccatag cctcaggtta
     3961 ctcatatata ctttagattg atttaaaact tcatttttaa tttaaaagga tctaggtgaa
     4021 gatccttttt gataatctca tgaccaaaat cccttaacgt gagttttcgt tccactgagc
     4081 gtcagacccc gtagaaaaga tcaaaggatc ttcttgagat cctttttttc tgcgcgtaat
     4141 ctgctgcttg caaacaaaaa aaccaccgct accagcggtg gtttgtttgc cggatcaaga
     4201 gctaccaact ctttttccga aggtaactgg cttcagcaga gcgcagatac caaatactgt
     4261 tcttctagtg tagccgtagt taggccacca cttcaagaac tctgtagcac cgcctacata
     4321 cctcgctctg ctaatcctgt taccagtggc tgctgccagt ggcgataagt cgtgtcttac
     4381 cgggttggac tcaagacgat agttaccgga taaggcgcag cggtcgggct gaacgggggg
     4441 ttcgtgcaca cagcccagct tggagcgaac gacctacacc gaactgagat acctacagcg
     4501 tgagctatga gaaagcgcca cgcttcccga agggagaaag gcggacaggt atccggtaag
     4561 cggcagggtc ggaacaggag agcgcacgag ggagcttcca gggggaaacg cctggtatct
     4621 ttatagtcct gtcgggtttc gccacctctg acttgagcgt cgatttttgt gatgctcgtc
     4681 aggggggcgg agcctatgga aaaacgccag caacgcggcc tttttacggt tcctggcctt
     4741 ttgctggcct tttgctcaca tgttctttcc tgcgttatcc cctgattctg tggataaccg
     4801 tattaccgcc atgcattagt tattaatagt aatcaattac ggggtcatta gttcatagcc
     4861 catatatgga gttccg
//
