LOCUS       sequence-176796-        8858 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses BFP, GFP, iRFP, and mRuby in response to combinations of 
            Cre and Flp recombinases.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 8858)
  AUTHORS   Weinberg BH, Pham NT, Caraballo LD, Lozanoski T, Engel A, Bhatia S, 
            Wong WW
  TITLE     Large-scale design of robust genetic circuits with multiple inputs 
            and outputs for mammalian cells.
  JOURNAL   Nat Biotechnol. 2017 Mar 27. doi: 10.1038/nbt.3805.
  PUBMED    28346402
REFERENCE   2  (bases 1 to 8858)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Biotechnol. 2017 Mar 27. doi: 10.1038/nbt.3805."
COMMENT     Sequence Label: pBW842_pCAG-pExpr-FP-DECODER
FEATURES             Location/Qualifiers
     source          1..8858
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     336..358
                     /label=pCAGGS-5
                     /note="Chimeric intron in CAG promoter, forward primer"
     primer_bind     420..439
                     /label=pCAG-F
                     /note="Rabbit beta-globin intron, for pCAG plasmids, 
                     forward primer"
     protein_bind    538..571
                     /label=FRT (minimal)
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="supports FLP-mediated excision but not integration 
                     (Turan and Bode, 2011)"
     protein_bind    complement(573..606)
                     /label=lox2272
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (AAGTATCC) (Shaw et al., 2021). lox2272 sites are 
                     compatible with each other, but incompatible with loxP or 
                     loxN sites (Lee and Saito, 1988)."
     CDS             622..1323
                     /codon_start=1
                     /product="monomeric blue fluorescent protein"
                     /label=TagBFP
                     /note="mammalian codon-optimized"
                     /translation="MSELIKENMHMKLYMEGTVDNHHFKCTSEGEGKPYEGTQTMRIKV
                     VEGGPLPFAFDILATSFLYGSKTFINHTQGIPDFFKQSFPEGFTWERVTTYEDGGVLTA
                     TQDTSLQDGCLIYNVKIRGVNFTSNGPVMQKKTLGWEAFTETLYPADGGLEGRNDMALK
                     LVGGSHLIANIKTTYRSKKPAKNLKMPGVYYVDYRLERIKEANNETYVEQHEVAVARYC
                     DLPSKLGHKLN"
     polyA_signal    1324..1548
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     protein_bind    complement(1669..1702)
                     /label=lox2272
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (AAGTATCC) (Shaw et al., 2021). lox2272 sites are 
                     compatible with each other, but incompatible with loxP or 
                     loxN sites (Lee and Saito, 1988)."
     regulatory      1712..1721
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(1718..1720,1721..1723,1724..2437)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             join(1718..1720,1721..1723,1724..2434)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(1763..1784)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(2024..2043)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     2371..2392
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     polyA_signal    2438..2662
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     protein_bind    2783..2816
                     /label=FRT (minimal)
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="supports FLP-mediated excision but not integration 
                     (Turan and Bode, 2011)"
     protein_bind    complement(2818..2851)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     regulatory      2861..2870
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             2867..3817
                     /codon_start=1
                     /product="near-infrared fluorescent protein with an 
                     emission peak at 720 nm, engineered from a bacterial 
                     phytochrome (Shcherbakova and Verkhusha, 2013)"
                     /label=iRFP720
                     /note="derived from Rhodopseudomonas palustris RpBphP2"
                     /translation="MAEGSVARQPDLLTCDDEPIHIPGAIQPHGLLLALAADMTIVAGS
                     DNLPELTGLAIGALIGRSAADVFDSETHNRLTIALAEPGAAVGAPITVGFTMRKDAGFI
                     GSWHRHDQLIFLELEPPQRDVAEPQAFFRRTNSAIRRLQAAETLESACAAAAQEVRKIT
                     GFDRVMIYRFASDFSGSVIAEDRCAEVESKLGLHYPASFIPAQARRLYTINPVRIIPDI
                     NYRPVPVTPDLNPVTGRPIDLSFAILRSVSPNHLEFMRNIGMHGTMSISILRGERLWGL
                     IVCHHRTPYYVDLDGRQACELVAQVLAWQIGVMEE"
     polyA_signal    3818..4042
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     protein_bind    complement(4163..4196)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     regulatory      4206..4215
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             4212..4922
                     /codon_start=1
                     /product="monomeric red fluorescent protein derived from 
                     mRuby, with improved photophysical properties (Lam et al., 
                     2012)"
                     /label=mRuby2
                     /translation="MVSKGEELIKENMRMKVVMEGSVNGHQFKCTGEGEGNPYMGTQTM
                     RIKVIEGGPLPFAFDILATSFMYGSRTFIKYPKGIPDFFKQSFPEGFTWERVTRYEDGG
                     VVTVMQDTSLEDGCLVYHVQVRGVNFPSNGPVMQKKTKGWEPNTEMMYPADGGLRGYTH
                     MALKVDGGGHLSCSFVTTYRSKKTVGNIKMPGIHAVDHRLERLEESDNEMFVVQREHAV
                     AKFAGLGGGMDELYK"
     polyA_signal    4926..5150
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     primer_bind     complement(5396..5415)
                     /label=Bglob-pA-R
                     /note="Rabbit beta-globin polyA region, reverse primer"
     polyA_signal    5461..5516
                     /label=beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal (Gil and 
                     Proudfoot, 1987)"
     primer_bind     complement(5515..5534)
                     /label=rbglobpA-R
                     /note="Rabbit beta-globin polyA, reverse primer. Also 
                     called rb-glob-pA-term-R"
     primer_bind     complement(5877..5893)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(5877..5893)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(5890..5912)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    5901..5917
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(5925..5931,5932..5949,5950..5955))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    5970..5991
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     rep_origin      6076..6210
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     polyA_signal    6230..6364
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(6267..6286)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     6321..6340
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(6432..6449)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(6603..7191)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(6683..6702)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(7362..8153,8154..8222))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     7985..8004
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(8223..8327)
                     /gene="bla"
                     /label=AmpR promoter
     enhancer        8358..8737
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
ORIGIN
        1 gcctcgggcc ggggagggct cgggggaggg gcgcggcggc ccccggagcg ccggcggctg
       61 tcgaggcgcg gcgagccgca gccattgcct tttatggtaa tcgtgcgaga gggcgcaggg
      121 acttcctttg tcccaaatct gtgcggagcc gaaatctggg aggcgccgcc gcaccccctc
      181 tagcgggcgc ggggcgaagc ggtgcggcgc cggcaggaag gaaatgggcg gggagggcct
      241 tcgtgcgtcg ccgcgccgcc gtccccttct ccctctccag cctcggggct gtccgcgggg
      301 ggacggctgc cttcgggggg gacggggcag ggcggggttc ggcttctggc gtgtgaccgg
      361 cggctctaga gcctctgcta accatgttca tgccttcttc tttttcctac agctcctggg
      421 caacgtgctg gttattgtgc tgtctcatca ttttggcaaa gaattgatta attcgagcga
      481 aggcgcgccc attactcgca tccattctca ggctgtctcg tctcgtctcg cacgcgtgaa
      541 gttcctattc tctagaaagt ataggaactt cgataacttc gtataggata ctttatacga
      601 agttataccg gtgccgccac catgagcgag ctgattaagg agaacatgca catgaagctg
      661 tacatggagg gcaccgtgga caaccatcac ttcaagtgca catccgaggg cgaaggcaag
      721 ccctacgagg gcacccagac catgagaatc aaggtggtcg agggcggccc tctccccttc
      781 gccttcgaca tcctggctac tagcttcctc tacggcagca agaccttcat caaccacacc
      841 cagggcatcc ccgacttctt caagcagtcc ttccctgagg gcttcacatg ggagagagtc
      901 accacatacg aagacggggg cgtgctgacc gctacccagg acaccagcct ccaggacggc
      961 tgcctcatct acaacgtcaa gatcagaggg gtgaacttca catccaacgg ccctgtgatg
     1021 cagaagaaaa cactcggctg ggaggccttc accgagacgc tgtaccccgc tgacggcggc
     1081 ctggaaggca gaaacgacat ggccctgaag ctcgtgggcg ggagccatct gatcgcaaac
     1141 atcaagacca catatagatc caagaaaccc gctaagaacc tcaagatgcc tggcgtctac
     1201 tatgtggact acagactgga aagaatcaag gaggccaaca acgagaccta cgtcgagcag
     1261 cacgaggtgg cagtggccag atactgcgac ctccctagca aactggggca caagcttaat
     1321 taactgtgcc ttctagttgc cagccatctg ttgtttgccc ctcccccgtg ccttccttga
     1381 ccctggaagg tgccactccc actgtccttt cctaataaaa tgaggaaatt gcatcgcatt
     1441 gtctgagtag gtgtcattct attctggggg gtggggtggg gcaggacagc aagggggagg
     1501 attgggaaga caatagcagg catgctgggg atgcggtggg ctctatggct tctgaggcgg
     1561 aaagaaccag ctggggctct agggggtatc cccacgcgcc ctgtagcgga attcggtacc
     1621 gctgggagtt cgtagacgga aacaaacgca gaatccaagc gcacgcgtat aacttcgtat
     1681 aggatacttt atacgaagtt ataccggtgc cgccaccatg gtgagcaagg gcgaggagct
     1741 gttcaccggg gtggtgccca tcctggtcga gctggacggc gacgtaaacg gccacaagtt
     1801 cagcgtgtcc ggcgagggcg agggcgatgc cacctacggc aagctgaccc tgaagttcat
     1861 ctgcaccacc ggcaagctgc ccgtgccctg gcccaccctc gtgaccaccc tgacctacgg
     1921 cgtgcagtgc ttcagccgct accccgacca catgaagcag cacgacttct tcaagtccgc
     1981 catgcccgaa ggctacgtcc aggagcgcac catcttcttc aaggacgacg gcaactacaa
     2041 gacccgcgcc gaggtgaagt tcgagggcga caccctggtg aaccgcatcg agctgaaggg
     2101 catcgacttc aaggaggacg gcaacatcct ggggcacaag ctggagtaca actacaacag
     2161 ccacaacgtc tatatcatgg ccgacaagca gaagaacggc atcaaggtga acttcaagat
     2221 ccgccacaac atcgaggacg gcagcgtgca gctcgccgac cactaccagc agaacacccc
     2281 catcggcgac ggccccgtgc tgctgcccga caaccactac ctgagcaccc agtccgccct
     2341 gagcaaagac cccaacgaga agcgcgatca catggtcctg ctggagttcg tgaccgccgc
     2401 cgggatcact ctcggcatgg acgagctgta caagtaactg tgccttctag ttgccagcca
     2461 tctgttgttt gcccctcccc cgtgccttcc ttgaccctgg aaggtgccac tcccactgtc
     2521 ctttcctaat aaaatgagga aattgcatcg cattgtctga gtaggtgtca ttctattctg
     2581 gggggtgggg tggggcagga cagcaagggg gaggattggg aagacaatag caggcatgct
     2641 ggggatgcgg tgggctctat ggcttctgag gcggaaagaa ccagctgggg ctctaggggg
     2701 tatccccacg cgccctgtag cggaattcgg taccgcactg aaggtcctca atcgcactgg
     2761 aaacatcaag gtcggcacgc gtgaagttcc tattctctag aaagtatagg aacttcgata
     2821 acttcgtata gcatacatta tacgaagtta taccggtgcc gccaccatgg cggaaggatc
     2881 cgtcgccagg cagcctgacc tcttgacctg cgacgatgag ccgatccata tccccggtgc
     2941 catccaaccg catggactgc tgctcgccct cgccgccgac atgacgatcg ttgccggcag
     3001 cgacaacctt cccgaactca ccggactggc gatcggcgcc ctgatcggcc gctctgcggc
     3061 cgatgtcttc gactcggaga cgcacaaccg tctgacgatc gccttggccg agcccggggc
     3121 ggccgtcgga gcaccgatca ctgtcggctt cacgatgcga aaggacgcag gcttcatcgg
     3181 ctcctggcat cgccatgatc agctcatctt cctcgagctc gagcctcccc agcgggacgt
     3241 cgccgagccg caggcgttct tccgccgcac caacagcgcc atccgccgcc tgcaggccgc
     3301 cgaaaccttg gaaagcgcct gcgccgccgc ggcgcaagag gtgcggaaga ttaccggctt
     3361 cgatcgggtg atgatctatc gcttcgcctc cgacttcagc gggtccgtga tcgcagagga
     3421 tcggtgcgcc gaggtcgagt caaaactagg cctgcactat cctgcctcat tcatcccggc
     3481 gcaggcccgt cggctctata ccatcaaccc ggtacggatc attcccgata tcaattatcg
     3541 gccggtgccg gtcaccccag acctcaatcc ggtcaccggg cggccgattg atcttagctt
     3601 cgccatcctg cgcagcgtct cgcccaacca tctggagttc atgcgcaaca taggcatgca
     3661 cggcacgatg tcgatctcga ttttgcgcgg cgagcgactg tggggattga tcgtttgcca
     3721 tcaccgaacg ccgtactacg tcgatctcga tggccgccaa gcctgcgagc tagtcgccca
     3781 ggttctggcc tggcagatcg gcgtgatgga agagtgactg tgccttctag ttgccagcca
     3841 tctgttgttt gcccctcccc cgtgccttcc ttgaccctgg aaggtgccac tcccactgtc
     3901 ctttcctaat aaaatgagga aattgcatcg cattgtctga gtaggtgtca ttctattctg
     3961 gggggtgggg tggggcagga cagcaagggg gaggattggg aagacaatag caggcatgct
     4021 ggggatgcgg tgggctctat ggcttctgag gcggaaagaa ccagctgggg ctctaggggg
     4081 tatccccacg cgccctgtag cggaattcgg taccctgacc tcctgccagc aatagtaaga
     4141 caacacgcaa agtcgcacgc gtataacttc gtatagcata cattatacga agttataccg
     4201 gtgccgccac catggtgtct aagggcgaag agctgatcaa ggaaaatatg cgtatgaagg
     4261 tggtcatgga aggttcggtc aacggccacc aattcaaatg cacaggtgaa ggagaaggca
     4321 atccgtacat gggaactcaa accatgagga tcaaagtcat cgagggagga cccctgccat
     4381 ttgcctttga cattcttgcc acgtcgttca tgtatggcag ccgtactttt atcaagtacc
     4441 cgaaaggcat tcctgatttc tttaaacagt cctttcctga gggttttact tgggaaagag
     4501 ttacgagata cgaagatggt ggagtcgtca ccgtcatgca ggacaccagc cttgaggatg
     4561 gctgtctcgt ttaccacgtc caagtcagag gggtaaactt tccctccaat ggtcccgtga
     4621 tgcagaagaa gaccaagggt tgggagccta atacagagat gatgtatcca gcagatggtg
     4681 gtctgagggg atacactcat atggcactga aagttgatgg tggtggccat ctgtcttgct
     4741 ctttcgtaac aacttacagg tcaaaaaaga ccgtcgggaa catcaagatg cccggtatcc
     4801 atgccgttga tcaccgcctg gaaaggttag aggaaagtga caatgaaatg ttcgtagtac
     4861 aacgcgaaca cgcagttgcc aagttcgccg ggcttggtgg tgggatggac gagctgtaca
     4921 agtaactgtg ccttctagtt gccagccatc tgttgtttgc ccctcccccg tgccttcctt
     4981 gaccctggaa ggtgccactc ccactgtcct ttcctaataa aatgaggaaa ttgcatcgca
     5041 ttgtctgagt aggtgtcatt ctattctggg gggtggggtg gggcaggaca gcaaggggga
     5101 ggattgggaa gacaatagca ggcatgctgg ggatgcggtg ggctctatgg cttctgaggc
     5161 ggaaagaacc agctggggct ctagggggta tccccacgcg ccctgtagcg gaattcggta
     5221 ccgagccaac tccctttaca acctcactca agtccgttag aggcggccgc ccaggataca
     5281 tagattacca caactccgag cccttccacc gctagcgcac tcctcaggtg caggctgcct
     5341 atcagaaggt ggtggctggt gtggccaatg ccctggctca caaataccac tgagatcttt
     5401 ttccctctgc caaaaattat ggggacatca tgaagcccct tgagcatctg acttctggct
     5461 aataaaggaa atttattttc attgcaatag tgtgttggaa ttttttgtgt ctctcactcg
     5521 gaaggacata tgggagggca aatcatttaa aacatcagaa tgagtatttg gtttagagtt
     5581 tggcaacata tgcccatatg ctggctgcca tgaacaaagg ttggctataa agaggtcatc
     5641 agtatatgaa acagccccct gctgtccatt ccttattcca tagaaaagcc ttgacttgag
     5701 gttagatttt ttttatattt tgttttgtgt tatttttttc tttaacatcc ctaaaatttt
     5761 ccttacatgt tttactagcc agatttttcc tcctctcctg actactccca gtcatagctg
     5821 tccctcttct cttatggaga tccctcgacc tgcagcccaa gcttggcgta atcatggtca
     5881 tagctgtttc ctgtgtgaaa ttgttatccg ctcacaattc cacacaacat acgagccgga
     5941 agcataaagt gtaaagcctg gggtgcctaa tgagtgagct aactcacatt aattgcgttg
     6001 cgctcactgc ccgctttcca gtcgggaaac ctgtcgtgcc agcggatccg catctcaatt
     6061 agtcagcaac catagtcccg cccctaactc cgcccagttc cgcccattct ccgccccatg
     6121 gctgactaat tttttttatt tatgcagagg ccgaggccgc ctcggcctct gagctattcc
     6181 agaagtagtg aggaggcttt tttggaggcc taggcttttg caaaaagcta acttgtttat
     6241 tgcagcttat aatggttaca aataaagcaa tagcatcaca aatttcacaa ataaagcatt
     6301 tttttcactg cattctagtt gtggtttgtc caaactcatc aatgtatctt atcatgtctg
     6361 gatccgctgc attaatgaat cggccaacgc gcggggagag gcggtttgcg tattgggcgc
     6421 tcttccgctt cctcgctcac tgactcgctg cgctcggtcg ttcggctgcg gcgagcggta
     6481 tcagctcact caaaggcggt aatacggtta tccacagaat caggggataa cgcaggaaag
     6541 aacatgtgag caaaaggcca gcaaaaggcc aggaaccgta aaaaggccgc gttgctggcg
     6601 tttttccata ggctccgccc ccctgacgag catcacaaaa atcgacgctc aagtcagagg
     6661 tggcgaaacc cgacaggact ataaagatac caggcgtttc cccctggaag ctccctcgtg
     6721 cgctctcctg ttccgaccct gccgcttacc ggatacctgt ccgcctttct cccttcggga
     6781 agcgtggcgc tttctcatag ctcacgctgt aggtatctca gttcggtgta ggtcgttcgc
     6841 tccaagctgg gctgtgtgca cgaacccccc gttcagcccg accgctgcgc cttatccggt
     6901 aactatcgtc ttgagtccaa cccggtaaga cacgacttat cgccactggc agcagccact
     6961 ggtaacagga ttagcagagc gaggtatgta ggcggtgcta cagagttctt gaagtggtgg
     7021 cctaactacg gctacactag aagaacagta tttggtatct gcgctctgct gaagccagtt
     7081 accttcggaa aaagagttgg tagctcttga tccggcaaac aaaccaccgc tggtagcggt
     7141 ggtttttttg tttgcaagca gcagattacg cgcagaaaaa aaggatctca agaagatcct
     7201 ttgatctttt ctacggggtc tgacgctcag tggaacgaaa actcacgtta agggattttg
     7261 gtcatgagat tatcaaaaag gatcttcacc tagatccttt taaattaaaa atgaagtttt
     7321 aaatcaatct aaagtatata tgagtaaact tggtctgaca gttaccaatg cttaatcagt
     7381 gaggcaccta tctcagcgat ctgtctattt cgttcatcca tagttgcctg actccccgtc
     7441 gtgtagataa ctacgatacg ggagggctta ccatctggcc ccagtgctgc aatgataccg
     7501 cgagacccac gctcaccggc tccagattta tcagcaataa accagccagc cggaagggcc
     7561 gagcgcagaa gtggtcctgc aactttatcc gcctccatcc agtctattaa ttgttgccgg
     7621 gaagctagag taagtagttc gccagttaat agtttgcgca acgttgttgc cattgctaca
     7681 ggcatcgtgg tgtcacgctc gtcgtttggt atggcttcat tcagctccgg ttcccaacga
     7741 tcaaggcgag ttacatgatc ccccatgttg tgcaaaaaag cggttagctc cttcggtcct
     7801 ccgatcgttg tcagaagtaa gttggccgca gtgttatcac tcatggttat ggcagcactg
     7861 cataattctc ttactgtcat gccatccgta agatgctttt ctgtgactgg tgagtactca
     7921 accaagtcat tctgagaata gtgtatgcgg cgaccgagtt gctcttgccc ggcgtcaata
     7981 cgggataata ccgcgccaca tagcagaact ttaaaagtgc tcatcattgg aaaacgttct
     8041 tcggggcgaa aactctcaag gatcttaccg ctgttgagat ccagttcgat gtaacccact
     8101 cgtgcaccca actgatcttc agcatctttt actttcacca gcgtttctgg gtgagcaaaa
     8161 acaggaaggc aaaatgccgc aaaaaaggga ataagggcga cacggaaatg ttgaatactc
     8221 atactcttcc tttttcaata ttattgaagc atttatcagg gttattgtct catgagcgga
     8281 tacatatttg aatgtattta gaaaaataaa caaatagggg ttccgcgcac atttccccga
     8341 aaagtgccac ctgggtcgac attgattatt gactagttat taatagtaat caattacggg
     8401 gtcattagtt catagcccat atatggagtt ccgcgttaca taacttacgg taaatggccc
     8461 gcctggctga ccgcccaacg acccccgccc attgacgtca ataatgacgt atgttcccat
     8521 agtaacgcca atagggactt tccattgacg tcaatgggtg gagtatttac ggtaaactgc
     8581 ccacttggca gtacatcaag tgtatcatat gccaagtacg ccccctattg acgtcaatga
     8641 cggtaaatgg cccgcctggc attatgccca gtacatgacc ttatgggact ttcctacttg
     8701 gcagtacatc tacgtattag tcatcgctat taccatggtc gaggtgagcc ccacgttctg
     8761 cttcactctc cccatctccc ccccctcccc acccccaatt ttgtatttat ttatttttta
     8821 attattttgt gcagcgatgg gggcgggggg gggggggg
//
