LOCUS       sequence-17680-e         654 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 654)
  AUTHORS   Sellers WR, Novitch BG, Miyake S, Heith A, Otterson GA, Kaye FJ, 
            Lassar AB, Kaelin WG
  TITLE     Stable binding to E2F is not required for the retinoblastoma protein
            to activate transcription, promote differentiation, and suppress 
            tumor cell growth.
  JOURNAL   Genes Dev. 1998 Jan 1. 12(1):95-106.
  PUBMED    9420334
REFERENCE   2  (bases 1 to 654)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Genes Dev. 
            1998 Jan 1. 12(1):95-106."
COMMENT     Sequence Label: 498 pSG5L HA RB del663 (NAAIRS)
FEATURES             Location/Qualifiers
     source          1..654
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     polyA_signal    204..325
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(241..260)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     295..314
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(359..376)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     primer_bind     complement(610..629)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 tattgaagga tcagatgaag cagatggaag taaacatctc ccaggagagt ccaaatttca
       61 gcagaaactg gcagaaatga cttctactcg aacacgaatg caaaagcaga aaatgaatga
      121 tagcatggat acctcaaaca aggaagagaa atgagaattc cccgatatcg cctcgagtct
      181 agatccagat cttattaaag cagaacttgt ttattgcagc ttataatggt tacaaataaa
      241 gcaatagcat cacaaatttc acaaataaag catttttttc actgcattct agttgtggtt
      301 tgtccaaact catcaatgta tcttatcatg tctggtcgac tctagactct tccgcttcct
      361 cgctcactga ctcgctgcgc tcggtcgttc ggctgcggcg agcggtatca gctcactcaa
      421 aggcggtaat acggttatcc acagaatcag gggataacgc aggaaagaac atgtgagcaa
      481 aaggccagca aaaggccagg aaccgtaaaa aggccgcgtt gctggcgttt ttccataggc
      541 tccgcccccc tgacgagcat cacaaaaatc gacgctcaag tcagaggtgg cgaaacccga
      601 caggactata aagataccag gcgtttcccc ctggaagctc cctcgtgcgc tctc
//
