LOCUS       sequence-176826-        9378 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Mammalian expression of human c-Met.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9378)
  AUTHORS   Tward AD, Jones KD, Yant S, Cheung ST, Fan ST, Chen X, Kay MA, Wang 
            R, Bishop JM
  TITLE     Distinct pathways of genomic progression to benign and malignant 
            tumors of the liver.
  JOURNAL   Proc Natl Acad Sci U S A. 2007 Sep 11;104(37):14771-6. Epub 2007 Sep
            4.
  PUBMED    17785413
REFERENCE   2  (bases 1 to 9378)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Proc Natl 
            Acad Sci U S A."; date: "2007-09-11"; volume: "104(37)"; pages: 
            "14771-6. Epub 2007 Sep 4"
COMMENT     Sequence Label: pT3-EF1aH c-Met
FEATURES             Location/Qualifiers
     source          1..9378
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        1..1179
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          232..1170
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     1127..1147
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     protein_bind    1210..1234
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(5485..5509)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     primer_bind     5537..5556
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(5591..5608)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    5597..5821
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     primer_bind     complement(6239..6255)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6239..6255)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(6252..6274)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    6263..6279
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(6287..6293,6294..6311,6312..6317))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    6332..6353
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(6470..6487)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(6641..7229)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(6721..6740)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(7400..8191,8192..8260))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     8023..8042
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(8261..8365)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     8433..8451
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     complement(8489..8511)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     8611..8630
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     8824..8846
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     8838..8855
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     8839..8855
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(9237..9257)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
ORIGIN
        1 ggctccggtg cccgtcagtg ggcagagcgc acatcgccca cagtccccga gaagttgggg
       61 ggaggggtcg gcaattgaac cggtgcctag agaaggtggc gcggggtaaa ctgggaaagt
      121 gatgtcgtgt actggctccg cctttttccc gagggtgggg gagaaccgta tataagtgca
      181 gtagtcgccg tgaacgttct ttttcgcaac gggtttgccg ccagaacaca ggtaagtgcc
      241 gtgtgtggtt cccgcgggcc tggcctcttt acgggttatg gcccttgcgt gccttgaatt
      301 acttccacct ggctgcagta cgtgattctt gatcccgagc ttcgggttgg aagtgggtgg
      361 gagagttcga ggccttgcgc ttaaggagcc ccttcgcctc gtgcttgagt tgaggcctgg
      421 cctgggcgct ggggccgccg cgtgcgaatc tggtggcacc ttcgcgcctg tctcgctgct
      481 ttcgataagt ctctagccat ttaaaatttt tgatgacctg ctgcgacgct ttttttctgg
      541 caagatagtc ttgtaaatgc gggccaagat ctgcacactg gtatttcggt ttttggggcc
      601 gcgggcggcg acggggcccg tgcgtcccag cgcacatgtt cggcgaggcg gggcctgcga
      661 gcgcggccac cgagaatcgg acgggggtag tctcaagctg gccggcctgc tctggtgcct
      721 ggcctcgcgc cgccgtgtat cgccccgccc tgggcggcaa ggctggcccg gtcggcacca
      781 gttgcgtgag cggaaagatg gccgcttccc ggccctgctg cagggagctc aaaatggagg
      841 acgcggcgct cgggagagcg ggcgggtgag tcacccacac aaaggaaaag ggcctttccg
      901 tcctcagccg tcgcttcatg tgactccacg gagtaccggg cgccgtccag gcacctcgat
      961 tagttctcga gcttttggag tacgtcgtct ttaggttggg gggaggggtt ttatgcgatg
     1021 gagtttcccc acactgagtg ggtggagact gaagttaggc cagcttggca cttgatgtaa
     1081 ttctccttgg aatttgccct ttttgagttt ggatcttggt tcattctcaa gcctcagaca
     1141 gtggttcaaa gtttttttct tccatttcag gtgtcgtgag gaattagctt ggtaccaata
     1201 cggatatcaa caagtttgta caaaaaagca ggctttaaag gaaccaattc agtcgactgg
     1261 atccggtacc gaattcgcgg ccgccaccat gaaggccccc gctgtgcttg cacctggcat
     1321 cctcgtgctc ctgtttacct tggtgcagag gagcaatggg gagtgtaaag aggcactagc
     1381 aaagtccgag atgaatgtga atatgaagta tcagcttccc aacttcaccg cggaaacacc
     1441 catccagaat gtcattctac atgagcatca cattttcctt ggtgccacta actacattta
     1501 tgttttaaat gaggaagacc ttcagaaggt tgctgagtac aagactgggc ctgtgctgga
     1561 acacccagat tgtttcccat gtcaggactg cagcagcaaa gccaatttat caggaggtgt
     1621 ttggaaagat aacatcaaca tggctctagt tgtcgacacc tactatgatg atcaactcat
     1681 tagctgtggc agcgtcaaca gagggacctg ccagcgacat gtctttcccc acaatcatac
     1741 tgctgacata cagtcggagg ttcactgcat attctcccca cagatagaag agcccagcca
     1801 gtgtcctgac tgtgtggtga gcgccctggg agccaaagtc ctttcatctg taaaggaccg
     1861 gttcatcaac ttctttgtag gcaataccat aaattcttct tatttcccag atcatccatt
     1921 gcattcgata tcagtgagaa ggctaaagga aacgaaagat ggttttatgt ttttgacgga
     1981 ccagtcctac attgatgttt tacctgagtt cagagattct taccccatta agtatgtcca
     2041 tgcctttgaa agcaacaatt ttatttactt cttgacggtc caaagggaaa ctctagatgc
     2101 tcagactttt cacacaagaa taatcaggtt ctgttccata aactctggat tgcattccta
     2161 catggaaatg cctctggagt gtattctcac agaaaagaga aaaaagagat ccacaaagaa
     2221 ggaagtgttt aatatacttc aggctgcgta tgtcagcaag cctggggccc agcttgctag
     2281 acaaatagga gccagcctga atgatgacat tcttttcggg gtgttcgcac aaagcaagcc
     2341 agattctgcc gaaccaatgg atcgatctgc catgtgtgca ttccctatca aatatgtcaa
     2401 cgacttcttc aacaagatcg tcaacaaaaa caatgtgaga tgtctccagc atttttacgg
     2461 acccaatcat gagcactgct ttaataggac acttctgaga aattcatcag gctgtgaagc
     2521 gcgccgtgat gaatatcgaa cagagtttac cacagctttg cagcgcgttg acttattcat
     2581 gggtcaattc agcgaagtcc tcttaacatc tatatccacc ttcattaaag gagacctcac
     2641 catagctaat cttgggacat cagagggtcg cttcatgcag gttgtggttt ctcgatcagg
     2701 accatcaacc cctcatgtga attttctcct ggactcccat ccagtgtctc cagaagtgat
     2761 tgtggagcat acattaaacc aaaatggcta cacactggtt atcactggga agaagatcac
     2821 gaagatccca ttgaatggct tgggctgcag acatttccag tcctgcagtc aatgcctctc
     2881 tgccccaccc tttgttcagt gtggctggtg ccacgacaaa tgtgtgcgat cggaggaatg
     2941 cctgagcggg acatggactc aacagatctg tctgcctgca atctacaagg ttttcccaaa
     3001 tagtgcaccc cttgaaggag ggacaaggct gaccatatgt ggctgggact ttggatttcg
     3061 gaggaataat aaatttgatt taaagaaaac tagagttctc cttggaaatg agagctgcac
     3121 cttgacttta agtgagagca cgatgaatac attgaaatgc acagttggtc ctgccatgaa
     3181 taagcatttc aatatgtcca taattatttc aaatggccac gggacaacac aatacagtac
     3241 attctcctat gtggatcctg taataacaag tatttcgccg aaatacggtc ctatggctgg
     3301 tggcacttta cttactttaa ctggaaatta cctaaacagt gggaattcta gacacatttc
     3361 aattggtgga aaaacatgta ctttaaaaag tgtgtcaaac agtattcttg aatgttatac
     3421 cccagcccaa accatttcaa ctgagtttgc tgttaaattg aaaattgact tagccaaccg
     3481 agagacaagc atcttcagtt accgtgaaga tcccattgtc tatgaaattc atccaaccaa
     3541 atcttttatt agtggtggga gcacaataac aggtgttggg aaaaacctga attcagttag
     3601 tgtcccgaga atggtcataa atgtgcatga agcaggaagg aactttacag tggcatgtca
     3661 acatcgctct aattcagaga taatctgttg taccactcct tccctgcaac agctgaatct
     3721 gcaactcccc ctgaaaacca aagccttttt catgttagat gggatccttt ccaaatactt
     3781 tgatctcatt tatgtacata atcctgtgtt taagcctttt gaaaagccag tgatgatctc
     3841 aatgggcaat gaaaatgtac tggaaattaa gggaaatgat attgaccctg aagcagttaa
     3901 aggtgaagtg ttaaaagttg gaaataagag ctgtgagaat atacacttac attctgaagc
     3961 cgttttatgc acggtcccca atgacctgct gaaattgaac agcgagctaa atatagagtg
     4021 gaagcaagca atttcttcaa ccgtccttgg aaaagtaata gttcaaccag atcagaattt
     4081 cacaggattg attgctggtg ttgtctcaat atcaacagca ctgttattac tacttgggtt
     4141 tttcctgtgg ctgaaaaaga gaaagcaaat taaagatctg ggcagtgaat tagttcgcta
     4201 cgatgcaaga gtacacactc ctcatttgga taggcttgta agtgcccgaa gtgtaagccc
     4261 aactacagaa atggtttcaa atgaatctgt agactaccga gctacttttc cagaagatca
     4321 gtttcctaat tcatctcaga acggttcatg ccgacaagtg cagtatcctc tgacagacat
     4381 gtcccccatc ctaactagtg gggactctga tatatccagt ccattactgc aaaatactgt
     4441 ccacattgac ctcagtgctc taaatccaga gctggtccag gcagtgcagc atgtagtgat
     4501 tgggcccagt agcctgattg tgcatttcaa tgaagtcata ggaagagggc attttggttg
     4561 tgtatatcat gggactttgt tggacaatga tggcaagaaa attcactgtg ctgtgaaatc
     4621 cttgaacaga atcactgaca taggagaagt ttcccaattt ctgaccgagg gaatcatcat
     4681 gaaagatttt agtcatccca atgtcctctc gctcctggga atctgcctgc gaagtgaagg
     4741 gtctccgctg gtggtcctac catacatgaa acatggagat cttcgaaatt tcattcgaaa
     4801 tgagactcat aatccaactg taaaagatct tattggcttt ggtcttcaag tagccaaagg
     4861 catgaaatat cttgcaagca aaaagtttgt ccacagagac ttggctgcaa gaaactgtat
     4921 gctggatgaa aaattcacag tcaaggttgc tgattttggt cttgccagag acatgtatga
     4981 taaagaatac tatagtgtac acaacaaaac aggtgcaaag ctgccagtga agtggatggc
     5041 tttggaaagt ctgcaaactc aaaagtttac caccaagtca gatgtgtggt cctttggcgt
     5101 gctcctctgg gagctgatga caagaggagc cccaccttat cctgacgtaa acacctttga
     5161 tataactgtt tacttgttgc aagggagaag actcctacaa cccgaatact gcccagaccc
     5221 cttatatgaa gtaatgctaa aatgctggca ccctaaagcc gaaatgcgcc catccttttc
     5281 tgaactggtg tcccggatat cagcgatctt ctctactttc attggggagc actatgtcca
     5341 tgtgaacgct acttatgtga acgtaaaatg tgtcgctccg tatccttctc tgttgtcatc
     5401 agaagataac gctgatgatg aggtggacac acgaccagcc tccttctggg agacatcata
     5461 ggcggccgca ctcgagatat ctagacccag ctttcttgta caaagtggtt gatatcgcgg
     5521 ccgctgcatt ctagttgtgg tttgtccaaa ctcatcaatg tatcttatca tgtctggatc
     5581 cgctgatcag cctcgactgt gccttctagt tgccagccat ctgttgtttg cccctccccc
     5641 gtgccttcct tgaccctgga aggtgccact cccactgtcc tttcctaata aaatgaggaa
     5701 attgcatcgc attgtctgag taggtgtcat tctattctgg ggggtggggt ggggcaggac
     5761 agcaaggggg aggattggga agacaatagc aggcatgctg gggatgcggt gggctctatg
     5821 gcttctgagg cgggatcccc ttgaaataca tccacaggta cacctccaat tgactcaaat
     5881 gatgtcaatt agtctatcag aagcttctaa agccatgaca tcattttctg gaattttcca
     5941 agctgtttaa aggcacagtc aacttagtgt atgtaaactt ctgacccact ggaattgtga
     6001 tacagtgaat tataagtgaa ataatctgtc tgtaaacaat tgttggaaaa atgacttgtg
     6061 tcatgcacaa agtagatgtc ctaactgact tgccaaaact attgtttgtt aacaagaaat
     6121 ttgtggagta gttgaaaaac gagttttaat gactccaact taagtgtatg taaacttccg
     6181 acttcaactg tataggtcta gagtcgacct gcaggcatgc aagcttggcg taatcatggt
     6241 catagctgtt tcctgtgtga aattgttatc cgctcacaat tccacacaac atacgagccg
     6301 gaagcataaa gtgtaaagcc tggggtgcct aatgagtgag ctaactcaca ttaattgcgt
     6361 tgcgctcact gcccgctttc cagtcgggaa acctgtcgtg ccagctgcat taatgaatcg
     6421 gccaacgcgc ggggagaggc ggtttgcgta ttgggcgctc ttccgcttcc tcgctcactg
     6481 actcgctgcg ctcggtcgtt cggctgcggc gagcggtatc agctcactca aaggcggtaa
     6541 tacggttatc cacagaatca ggggataacg caggaaagaa catgtgagca aaaggccagc
     6601 aaaaggccag gaaccgtaaa aaggccgcgt tgctggcgtt tttccatagg ctccgccccc
     6661 ctgacgagca tcacaaaaat cgacgctcaa gtcagaggtg gcgaaacccg acaggactat
     6721 aaagatacca ggcgtttccc cctggaagct ccctcgtgcg ctctcctgtt ccgaccctgc
     6781 cgcttaccgg atacctgtcc gcctttctcc cttcgggaag cgtggcgctt tctcatagct
     6841 cacgctgtag gtatctcagt tcggtgtagg tcgttcgctc caagctgggc tgtgtgcacg
     6901 aaccccccgt tcagcccgac cgctgcgcct tatccggtaa ctatcgtctt gagtccaacc
     6961 cggtaagaca cgacttatcg ccactggcag cagccactgg taacaggatt agcagagcga
     7021 ggtatgtagg cggtgctaca gagttcttga agtggtggcc taactacggc tacactagaa
     7081 gaacagtatt tggtatctgc gctctgctga agccagttac cttcggaaaa agagttggta
     7141 gctcttgatc cggcaaacaa accaccgctg gtagcggtgg tttttttgtt tgcaagcagc
     7201 agattacgcg cagaaaaaaa ggatctcaag aagatccttt gatcttttct acggggtctg
     7261 acgctcagtg gaacgaaaac tcacgttaag ggattttggt catgagatta tcaaaaagga
     7321 tcttcaccta gatcctttta aattaaaaat gaagttttaa atcaatctaa agtatatatg
     7381 agtaaacttg gtctgacagt taccaatgct taatcagtga ggcacctatc tcagcgatct
     7441 gtctatttcg ttcatccata gttgcctgac tccccgtcgt gtagataact acgatacggg
     7501 agggcttacc atctggcccc agtgctgcaa tgataccgcg agacccacgc tcaccggctc
     7561 cagatttatc agcaataaac cagccagccg gaagggccga gcgcagaagt ggtcctgcaa
     7621 ctttatccgc ctccatccag tctattaatt gttgccggga agctagagta agtagttcgc
     7681 cagttaatag tttgcgcaac gttgttgcca ttgctacagg catcgtggtg tcacgctcgt
     7741 cgtttggtat ggcttcattc agctccggtt cccaacgatc aaggcgagtt acatgatccc
     7801 ccatgttgtg caaaaaagcg gttagctcct tcggtcctcc gatcgttgtc agaagtaagt
     7861 tggccgcagt gttatcactc atggttatgg cagcactgca taattctctt actgtcatgc
     7921 catccgtaag atgcttttct gtgactggtg agtactcaac caagtcattc tgagaatagt
     7981 gtatgcggcg accgagttgc tcttgcccgg cgtcaatacg ggataatacc gcgccacata
     8041 gcagaacttt aaaagtgctc atcattggaa aacgttcttc ggggcgaaaa ctctcaagga
     8101 tcttaccgct gttgagatcc agttcgatgt aacccactcg tgcacccaac tgatcttcag
     8161 catcttttac tttcaccagc gtttctgggt gagcaaaaac aggaaggcaa aatgccgcaa
     8221 aaaagggaat aagggcgaca cggaaatgtt gaatactcat actcttcctt tttcaatatt
     8281 attgaagcat ttatcagggt tattgtctca tgagcggata catatttgaa tgtatttaga
     8341 aaaataaaca aataggggtt ccgcgcacat ttccccgaaa agtgccacct gacgtctaag
     8401 aaaccattat tatcatgaca ttaacctata aaaataggcg tatcacgagg ccctttcgtc
     8461 tcgcgcgttt cggtgatgac ggtgaaaacc tctgacacat gcagctcccg gagacggtca
     8521 cagcttgtct gtaagcggat gccgggagca gacaagcccg tcagggcgcg tcagcgggtg
     8581 ttggcgggtg tcggggctgg cttaactatg cggcatcaga gcagattgta ctgagagtgc
     8641 accatatgcg gtgtgaaata ccgcacagat gcgtaaggag aaaataccgc atcaggcgcc
     8701 attcgccatt caggctgcgc aactgttggg aagggcgatc ggtgcgggcc tcttcgctat
     8761 tacgccagct ggcgaaaggg ggatgtgctg caaggcgatt aagttgggta acgccagggt
     8821 tttcccagtc acgacgttgt aaaacgacgg ccagtgaatt cgagctcggt accctacagt
     8881 tgaagtcgga agtttacata cacttaagtt ggagtcatta aaactcgttt ttcaactact
     8941 ccacaaattt cttgttaaca aacaatagtt ttggcaagtc agttaggaca tctactttgt
     9001 gcatgacaca agtcattttt ccaacaattg tttacagaca gattatttca cttataattc
     9061 actgtatcac aattccagtg ggtcagaagt ttacatacac taagttgact gtgcctttaa
     9121 acagcttgga aaattccaga aaatgatgtc atggctttag aagcttctga tagactaatt
     9181 gacatcattt gagtcaattg gaggtgtacc tgtggatgta tttcaaggaa ttctgtggaa
     9241 tgtgtgtcag ttagggtgtg gaaagtcccc aggctcccca gcaggcagaa gtatgcaaag
     9301 catgcatatc gatactagtt taattaagat cctctttgca gctaatggac cttctaggtc
     9361 ttgaaaggag tgggaatt
//
