LOCUS       sequence-176843-        9884 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Donor vector for creation of CRISPR/Cas9-mediated Bxb1 attP-flanked 
            alleles..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9884)
  AUTHORS   Voutev R, Mann RS
  TITLE     Bxb1 phage recombinase assists genome engineering in Drosophila 
            melanogaster.
  JOURNAL   Biotechniques. 2017 Jan 1;62(1):37-38. doi: 10.2144/000114494.
  PUBMED    28118814
REFERENCE   2  (bases 1 to 9884)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10"; journalName: 
            "Biotechniques"; date: "2017-01-1- 1"; volume: "62"; issue: "1"; 
            pages: "37-38"
COMMENT     Sequence Label: pRVV653
FEATURES             Location/Qualifiers
     source          1..9884
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      complement(30..458)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(140..161)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     352..371
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     585..607
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     599..616
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     600..616
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     623..642
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        623..641
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(675..693)
                     /label=pCasper-F
                     /note="Drosophila mini-white gene, reverse primer"
     CDS             join(1145..1216,1618..1891,1966..2620,2682..2997,3201..3332,
                     3403..4017)
                     /codon_start=1
                     /gene="white"
                     /product="Drosophila white gene eye color pigment"
                     /label=mini-white
                     /note="This is a modified version of the white gene lacking
                     part of the first intron."
                     /translation="MGQEDQELLIRGGSKHPSAEHLNNGDSGAASQSCINQGFGQAKNY
                     GTLRPPSPPEDSGSGSGQLAENLTYAWHNMDIFGAVNQPGSGWRQLVNRTRGLFCNERH
                     IPAPRKHLLKNVCGVAYPGELLAVMGSSGAGKTTLLNALAFRSPQGIQVSPSGMRLLNG
                     QPVDAKEMQARCAYVQQDDLFIGSLTAREHLIFQAMVRMPRHLTYRQRVARVDQVIQEL
                     SLSKCQHTIIGVPGRVKGLSGGERKRLAFASEALTDPPLLICDEPTSGLDSFTAHSVVQ
                     VLKKLSQKGKTVILTIHQPSSELFELFDKILLMAEGRVAFLGTPSEAVDFFSYVGAQCP
                     TNYNPADFYVQVLAVVPGREIESRDRIAKICDNFAISKVARDMEQLLATKNLEKPLEQP
                     ENGYTYKATWFMQFRAVLWRSWLSVLKEPLLVKVRLIQTTMVAILIGLIFLGQQLTQVG
                     VMNINGAIFLFLTNMTFQNVFATINVFTSELPVFMREARSRLYRCDTYFLGKTIAELPL
                     FLTVPLVFTAIAYPMIGLRAGVLHFFNCLALVTLVANVSTSFGYLISCASSSTSMALSV
                     GPPVIIPFLLFGGFFLNSGSVPVYLKWLSYLSWFRYANEGLLINQWADVEPGEISCTSS
                     NTTCPSSGKVILETLNFSAADLPLDYVGLAILIVSFRVLAYLALRLRARRKE"
     protein_bind    4845..4892
                     /label=Bxb1 attP
                     /bound_moiety="Mycobacteriophage Bxb1 integrase"
                     /note="Minimal attP site for the Mycopbacteriophage Bxb1 
                     integrase (Voutev and Mann, 2017)"
     protein_bind    6739..6786
                     /label=Bxb1 attP
                     /bound_moiety="Mycobacteriophage Bxb1 integrase"
                     /note="Minimal attP site for the Mycopbacteriophage Bxb1 
                     integrase (Voutev and Mann, 2017)"
     intron          7049..7114
                     /label=small t intron
                     /note="SV40 (simian virus 40) small t antigen intron"
     CDS             7244..7264
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     polyA_signal    7536..7670
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(7573..7592)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     7627..7646
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     promoter        complement(7696..7714)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(7735..7751)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(7735..7751)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(7748..7770)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    7759..7775
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(7783..7789,7790..7807,7808..7813))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7828..7849
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(7966..7983)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(8137..8725)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(8217..8236)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(8896..9687,9688..9756))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     9519..9538
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(9757..9861)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 ctaaattgta agcgttaata ttttgttaaa attcgcgtta aatttttgtt aaatcagctc
       61 attttttaac caataggccg aaatcggcaa aatcccttat aaatcaaaag aatagaccga
      121 gatagggttg agtgttgttc cagtttggaa caagagtcca ctattaaaga acgtggactc
      181 caacgtcaaa gggcgaaaaa ccgtctatca gggcgatggc ccactacgtg aaccatcacc
      241 ctaatcaagt tttttggggt cgaggtgccg taaagcacta aatcggaacc ctaaagggag
      301 cccccgattt agagcttgac ggggaaagcc ggcgaacgtg gcgagaaagg aagggaagaa
      361 agcgaaagga gcgggcgcta gggcgctggc aagtgtagcg gtcacgctgc gcgtaaccac
      421 cacacccgcc gcgcttaatg cgccgctaca gggcgcgtcc cattcgccat tcaggctgcg
      481 caactgttgg gaagggcgat cggtgcgggc ctcttcgcta ttacgccagc tggcgaaagg
      541 gggatgtgct gcaaggcgat taagttgggt aacgccaggg ttttcccagt cacgacgttg
      601 taaaacgacg gccagtgaat tgtaatacga ctcactatag ggcgaattgg gtacgtaccg
      661 ggcccctagt atgtatgtaa gttaataaaa cccatttttg cggaaagtag ataaaaaaaa
      721 catttttttt ttttactgca ctggatatca ttgaacttat ctgatcagtt ttaaatttac
      781 ttcgatccaa gggtatttga tgtaccaggt tctttcgatt acctctcact caaaatgaca
      841 ttccactcaa agtcagcgct gtttgcctcc ttctctgtcc acagaaatat cgccgtctct
      901 ttcgccgctg cgtccgctat ctctttcgcc accgtttgta gcgttacgta gcgtcaatgt
      961 ccgccttcag ttgcattttg tcagcggttt cgtgacgaag ctccaagcgg tttacgccat
     1021 caattaaaca caaagtgctg tgccaaaact cctctcgctt cttatttttg tttgtttttt
     1081 gagtgattgg ggtggtgatt ggttttgggt gggtaagcag gggaaagtgt gaaaaatccc
     1141 ggcaatgggc caagaggatc aggagctatt aattcgcgga ggcagcaaac acccatctgc
     1201 cgagcatctg aacaatgtga gtagtacatg tgcatacatc ttaagttcac ttgatctata
     1261 ggaactgcga ttgcaacatc aaattgtctg cggcgtgaga actgcgaccc acaaaaatcc
     1321 caaaccgcaa ttgcacaaac aaatagtgac acgaaacaga ttattctggt agctgttctc
     1381 gctatataag acaatttttg agatcatatc atgatcaaga catctaaagg cattcatttt
     1441 cgactatatt cttttttaca aaaaatataa caaccagata ttttaagctg atcctagatg
     1501 cacaaaaaat aaataaaagt ataaacctac ttcgtaggat acttcggggt actttttgtt
     1561 cggggttaga tgagcataac gcttgtagtt gatatttgag atcccctatc attgcagggt
     1621 gacagcggag cggcttcgca gagctgcatt aaccagggct tcgggcaggc caaaaactac
     1681 ggcacgctcc ggccacccag tccgccggag gactccggtt cagggagcgg ccaactagcc
     1741 gagaacctca cctatgcctg gcacaatatg gacatctttg gggcggtcaa tcagccgggc
     1801 tccggatggc ggcagctggt caaccggaca cgcggactat tctgcaacga gcgacacata
     1861 ccggcgccca ggaaacattt gctcaagaac ggtgagtttc tattcgcagt cggctgatct
     1921 gtgtgaaatc ttaataaagg gtccaattac caatttgaaa ctcagtttgc ggcgtggcct
     1981 atccgggcga acttttggcc gtgatgggca gttccggtgc cggaaagacg accctgctga
     2041 atgcccttgc ctttcgatcg ccgcagggca tccaagtatc gccatccggg atgcgactgc
     2101 tcaatggcca acctgtggac gccaaggaga tgcaggccag gtgcgcctat gtccagcagg
     2161 atgacctctt tatcggctcc ctaacggcca gggaacacct gattttccaa gccatggtgc
     2221 ggatgccacg acatctgacc tatcggcagc gagtggcccg cgtggatcag gtgatccagg
     2281 agctttcgct cagcaaatgt cagcacacga tcatcggtgt gcccggcagg gtgaaaggtc
     2341 tgtccggcgg agaaaggaag cgtctggcat tcgcctccga ggctctaacc gatccgccgc
     2401 ttctgatctg cgatgagccc acctccggac tggactcctt taccgcccac agcgtcgtcc
     2461 aggtgctgaa gaagctgtcg cagaagggca agaccgtcat cctgaccatt catcagccgt
     2521 cttccgagct gtttgagctc tttgacaaga tccttctgat ggccgagggc agggtagctt
     2581 tcttgggcac tcccagcgaa gccgtcgact tcttttccta gtgagttcga tgtgtttatt
     2641 aagggtatct agtattacat aacatctcaa ctcctatcca gcgtgggtgc ccagtgtcct
     2701 accaactaca atccggcgga cttttacgta caggtgttgg ccgttgtgcc cggacgggag
     2761 atcgagtccc gtgatcggat cgccaagata tgcgacaatt ttgccattag caaagtagcc
     2821 cgggatatgg agcagttgtt ggccaccaaa aatctggaga agccactgga gcagccggag
     2881 aatgggtaca cctacaaggc cacctggttc atgcagttcc gggcggtcct gtggcgatcc
     2941 tggctgtcgg tgctcaagga accactcctc gtaaaagtgc gacttattca gacaacggtg
     3001 agtggttcca gtggaaacaa atgatataac gcttacaatt cttggaaaca aattcgctag
     3061 attttagata gaattgcctg attccacacc cttcttagtt tttttcaatg agatgtatag
     3121 tttatagttt tgcagaagat aaataaattt catttaactc gcgaatatta atgagatgcg
     3181 agtaacattt taatttgcag atggttgcca tcttgattgg cctcatcttt ttgggccaac
     3241 aactcacgca agtgggtgtg atgaatatca acggagccat cttcctcttc ctgaccaaca
     3301 tgacctttca aaacgtcttt gccacgataa atgtaagtca tgtttagaat acatttgcat
     3361 ttcaataatt tactaacttt ctaatgaatc gattcgattt aggtgttcac ctcagagctg
     3421 ccagttttta tgagggaggc ccgaagtcga ctttatcgct gtgacacata ctttctgggc
     3481 aaaacgattg ccgaattgcc gctttttctc acagtgccac tggtcttcac ggcgattgcc
     3541 tatccgatga tcggactgcg ggccggagtg ctgcacttct tcaactgcct ggcgctggtc
     3601 actctggtgg ccaatgtgtc aacgtccttc ggatatctaa tatcctgcgc cagctcctcg
     3661 acctcgatgg cgctgtctgt gggtccgccg gttatcatac cattcctgct ctttggcggc
     3721 ttcttcttga actcgggctc ggtgccagta tacctcaaat ggttgtcgta cctctcatgg
     3781 ttccgttacg ccaacgaggg tctgctgatt aaccaatggg cggacgtgga gccgggcgaa
     3841 attagctgca catcgtcgaa caccacgtgc cccagttcgg gcaaggtcat cctggagacg
     3901 cttaacttct ccgccgccga tctgccgctg gactacgtgg gtctggccat tctcatcgtg
     3961 agcttccggg tgctcgcata tctggctcta agacttcggg cccgacgcaa ggagtagccg
     4021 acatatatcc gaaataactg cttgtttttt ttttttacca ttattaccat cgtgtttact
     4081 gtttattgcc ccctcaaaaa gctaatgtaa ttatatttgt gccaataaaa acaagatatg
     4141 acctatagaa tacaagtatt tccccttcga acatccccac aagtagactt tggatttgtc
     4201 ttctaaccaa aagacttaca cacctgcata ccttacatca aaaactcgtt tatcgctaca
     4261 taaaacaccg ggatatattt tttatataca tacttttcaa atcgcgcgcc ctcttcataa
     4321 ttcacctcca ccacaccacg tttcgtagtt gctctttcgc tgtctcccac ccgctctccg
     4381 caacacattc accttttgtt cgacgacctt ggagcgactg tcgttagttc cgcgcgattc
     4441 ggttcgctca aatggttccg agtggttcat ttcgtctcaa tagaaattag taataaatat
     4501 ttgtatgtac aatttatttg ctccaatata tttgtatata tttccctcac agctatattt
     4561 attctaattt aatattatga ctttttaagg taattttttg tgacctgttc ggagtgatta
     4621 gcgttacaat ttgaactgaa agtgacatcc agtgtttgtt ccttgtgtag atgcatctca
     4681 aaaaaatggt gggcataata gtgttgttta tatatatcaa aaataacaac tataataata
     4741 agaatacatt taatttagaa aatgcttgga tttcactgga actaggctag atttaaatgc
     4801 atgccctgca ggaagcttcc taggaggcct agatctgcgg ccgcggtttg taccgtacac
     4861 cactgagacc gcggtggttg accagacaaa ccttaattaa gaagttccta ttcttcaaaa
     4921 ggtataggaa cttcccgcga gctcgcccgg ggatctaatt caattagaga ctaattcaat
     4981 tagagctaat tcaattagga tccaagctta tcgatttcga accctcgacc gccggagtat
     5041 aaatagaggc gcttcgtcta cggagcgaca attcaattca aacaagcaaa gtgaacacgt
     5101 cgctaagcga aagctaagca aataaacaag cgcagctgaa caagctaaac aatcgggcgg
     5161 ccgcactaga gccggtcgcc accatgaggt cttccaagaa tgttatcaag gagttcatga
     5221 ggtttaaggt tcgcatggaa ggaacggtca atgggcacga gtttgaaata gaaggcgaag
     5281 gagaggggag gccatacgaa ggccacaata ccgtaaagct taaggtaacc aaggggggac
     5341 ctttgccatt tgcttgggat attttgtcac cacaatttca gtatggaagc aaggtatatg
     5401 tcaagcaccc tgccgacata ccagactata aaaagctgtc atttcctgaa ggatttaaat
     5461 gggaaagggt catgaacttt gaagacggtg gcgtcgttac tgtaacccag gattccagtt
     5521 tgcaggatgg ctgtttcatc tacaaggtca agttcattgg cgtgaacttt ccttccgatg
     5581 gacctgttat gcaaaagaag acaatgggct gggaagccag cactgagcgt ttgtatcctc
     5641 gtgatggcgt gttgaaagga gagattcata aggctctgaa gctgaaagac ggtggtcatt
     5701 acctagttga attcaaaagt atttacatgg caaagaagcc tgtgcagcta ccagggtact
     5761 actatgttga ctccaaactg gatataacaa gccacaacga agactataca atcgttgagc
     5821 agtatgaaag aaccgaggga cgccaccatc tgttccttta gcggccatcg aattcgagct
     5881 cgcccactaa gcgtcgcgcc acttcaacgc tcgatgggag cgtcattggt gggcggggta
     5941 accgtcgaaa tcagtgttta cgcttccaat cgcaacaaaa aattcactgc aacactgaaa
     6001 agcatacgaa aacgatgaag attgtacgag aaaccataaa gtattttatc cacaaagaca
     6061 cgtatagcag aaaagccaag ttaactcggc gataagttgt gtacacaaga ataaaatcgg
     6121 ccagattcag tgttgtcaga aataagaaaa ccccactatg tttttctttg ccttttcttt
     6181 ctcccagcga tcattcattt cgtggtgaaa gaacggggtc attgcacgga gtttcgactg
     6241 cgggaaagca gagctgccgt tcacttcgtc tataattagc gctttctatt ttccccgatt
     6301 cgggccgctg ctgcgctttt ccgcctgctg tttgtggcaa gtgtagcagc aggctgtgca
     6361 cgcagtgtgg catgcacttg gctttccacc gttggtatcg attctctggg acgatgagtc
     6421 attcctttcg gggccacagc ataatcgttg ccagctcacc gaaatggtga cttcatttct
     6481 taactgccgt caagcatgcg attgtacata catacatatt tatatatgta catatttatg
     6541 tgactatggt aggtcgatat aatagcaatc aacgcaagca aatgtgtcag tcctgcttac
     6601 aggaacgatt ctatttagta attttcgttg tataaagtaa ttatgtatgt atgtaagccc
     6661 cataaatctg aaacaattag gcaaaaccat gcgaagctga agttcctatt cttcaaaagg
     6721 tataggaact tcaccggtgg tttgtctggt caaccaccgc ggtctcagtg gtgtacggta
     6781 caaaccggcg cgccgctagc catatgggga ttggtggcga cgactcctgg agcccgtcag
     6841 tatcggcgga attacagctg agcgccggtc gctaccatta ccagttggtc tggtgtcaaa
     6901 aataataata accgggcagg ccatgtctgc ccgtatttcg cgtaaggaaa tccctcgagg
     6961 gtacctctag aggatctttg tgaaggaacc ttacttctgt ggtgtgacat aattggacaa
     7021 actacctaca gagatttaaa gctctaaggt aaatataaaa tttttaagtg tataatgtgt
     7081 taaactactg attctaattg tttgtgtatt ttagattcca acctatggaa ctgatgaatg
     7141 ggagcagtgg tggaatgcct ttaatgagga aaacctgttt tgctcagaag aaatgccatc
     7201 tagtgatgat gaggctactg ctgactctca acattctact cctccaaaaa agaagagaaa
     7261 ggtagaagac cccaaggact ttccttcaga attgctaagt tttttgagtc atgctgtgtt
     7321 tagtaataga actcttgctt gctttgctat ttacaccaca aaggaaaaag ctgcactgct
     7381 atacaagaaa attatggaaa aatatttgat gtatagtgcc ttgactagag atcataatca
     7441 gccataccac atttgtagag gttttacttg ctttaaaaaa cctcccacac ctccccctga
     7501 acctgaaaca taaaatgaat gcaattgttg ttgttaactt gtttattgca gcttataatg
     7561 gttacaaata aagcaatagc atcacaaatt tcacaaataa agcatttttt tcactgcatt
     7621 ctagttgtgg tttgtccaaa ctcatcaatg tatcttatca tgtctggatc cactagttct
     7681 agccagcttt tgttcccttt agtgagggtt aatttcgagc ttggcgtaat catggtcata
     7741 gctgtttcct gtgtgaaatt gttatccgct cacaattcca cacaacatac gagccggaag
     7801 cataaagtgt aaagcctggg gtgcctaatg agtgagctaa ctcacattaa ttgcgttgcg
     7861 ctcactgccc gctttccagt cgggaaacct gtcgtgccag ctgcattaat gaatcggcca
     7921 acgcgcgggg agaggcggtt tgcgtattgg gcgctcttcc gcttcctcgc tcactgactc
     7981 gctgcgctcg gtcgttcggc tgcggcgagc ggtatcagct cactcaaagg cggtaatacg
     8041 gttatccaca gaatcagggg ataacgcagg aaagaacatg tgagcaaaag gccagcaaaa
     8101 ggccaggaac cgtaaaaagg ccgcgttgct ggcgtttttc cataggctcc gcccccctga
     8161 cgagcatcac aaaaatcgac gctcaagtca gaggtggcga aacccgacag gactataaag
     8221 ataccaggcg tttccccctg gaagctccct cgtgcgctct cctgttccga ccctgccgct
     8281 taccggatac ctgtccgcct ttctcccttc gggaagcgtg gcgctttctc atagctcacg
     8341 ctgtaggtat ctcagttcgg tgtaggtcgt tcgctccaag ctgggctgtg tgcacgaacc
     8401 ccccgttcag cccgaccgct gcgccttatc cggtaactat cgtcttgagt ccaacccggt
     8461 aagacacgac ttatcgccac tggcagcagc cactggtaac aggattagca gagcgaggta
     8521 tgtaggcggt gctacagagt tcttgaagtg gtggcctaac tacggctaca ctagaagaac
     8581 agtatttggt atctgcgctc tgctgaagcc agttaccttc ggaaaaagag ttggtagctc
     8641 ttgatccggc aaacaaacca ccgctggtag cggtggtttt tttgtttgca agcagcagat
     8701 tacgcgcaga aaaaaaggat ctcaagaaga tcctttgatc ttttctacgg ggtctgacgc
     8761 tcagtggaac gaaaactcac gttaagggat tttggtcatg agattatcaa aaaggatctt
     8821 cacctagatc cttttaaatt aaaaatgaag ttttaaatca atctaaagta tatatgagta
     8881 aacttggtct gacagttacc aatgcttaat cagtgaggca cctatctcag cgatctgtct
     8941 atttcgttca tccatagttg cctgactccc cgtcgtgtag ataactacga tacgggaggg
     9001 cttaccatct ggccccagtg ctgcaatgat accgcgagac ccacgctcac cggctccaga
     9061 tttatcagca ataaaccagc cagccggaag ggccgagcgc agaagtggtc ctgcaacttt
     9121 atccgcctcc atccagtcta ttaattgttg ccgggaagct agagtaagta gttcgccagt
     9181 taatagtttg cgcaacgttg ttgccattgc tacaggcatc gtggtgtcac gctcgtcgtt
     9241 tggtatggct tcattcagct ccggttccca acgatcaagg cgagttacat gatcccccat
     9301 gttgtgcaaa aaagcggtta gctccttcgg tcctccgatc gttgtcagaa gtaagttggc
     9361 cgcagtgtta tcactcatgg ttatggcagc actgcataat tctcttactg tcatgccatc
     9421 cgtaagatgc ttttctgtga ctggtgagta ctcaaccaag tcattctgag aatagtgtat
     9481 gcggcgaccg agttgctctt gcccggcgtc aatacgggat aataccgcgc cacatagcag
     9541 aactttaaaa gtgctcatca ttggaaaacg ttcttcgggg cgaaaactct caaggatctt
     9601 accgctgttg agatccagtt cgatgtaacc cactcgtgca cccaactgat cttcagcatc
     9661 ttttactttc accagcgttt ctgggtgagc aaaaacagga aggcaaaatg ccgcaaaaaa
     9721 gggaataagg gcgacacgga aatgttgaat actcatactc ttcctttttc aatattattg
     9781 aagcatttat cagggttatt gtctcatgag cggatacata tttgaatgta tttagaaaaa
     9841 taaacaaata ggggttccgc gcacatttcc ccgaaaagtg ccac
//
