LOCUS       sequence-176861-       10064 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Luciferase reporter plasmid targeted at csr-1 locus.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10064)
  AUTHORS   Matsu-Ura T, Baek M, Kwon J, Hong C
  TITLE     Efficient gene editing in Neurospora crassa with CRISPR technology.
  JOURNAL   Fungal Biol Biotechnol. 2015 Jul 15;2:4. doi: 
            10.1186/s40694-015-0015-1. eCollection 2015.
  PUBMED    28955455
REFERENCE   2  (bases 1 to 10064)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Fungal Biol
            Biotechnol."; date: "2015-07-15"; volume: "2:4. doi"; pages: " 
            10.1186/s40694-015-0015-1. eCollection 2015"
COMMENT     Sequence Label: M249-5'csr-1-Pgsy-1-luciferase-Bar-3'csr-1
FEATURES             Location/Qualifiers
     source          1..10064
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(29..51)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     151..170
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     promoter        194..408
                     /gene="S. cerevisiae URA3"
                     /label=URA3 promoter
     CDS             complement(4540..5091)
                     /codon_start=1
                     /gene="Streptomyces hygroscopicus bar"
                     /product="phosphinothricin acetyltransferase"
                     /label=BlpR
                     /note="confers resistance to bialophos or phosphinothricin"
                     /translation="MSPERRPADIRRATEADMPAVCTIVNHYIETSTVNFRTEPQEPQE
                     WTDDLVRLRERYPWLVAEVDGEVAGIAYAGPWKARNAYDWTAESTVYVSPRHQRTGLGS
                     TLYTHLLKSLEAQGFKSVVAVIGLPNDPSVRMHEALGYAPRGMLRAAGFKHGNWHDVGF
                     WQLDFSLPVPPRPVLPVTEI"
     promoter        complement(5114..5473)
                     /label=trpC promoter
                     /note="promoter for Aspergillus nidulans trpC"
     primer_bind     complement(6514..6536)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    6525..6541
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(6549..6555,6556..6573,6574..6579))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    6594..6615
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(6732..6749)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(6903..7491)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(6983..7002)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(7662..8453,8454..8522))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     8285..8304
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(8523..8627)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      8654..9996
                     /label=2u ori
                     /note="yeast 2u plasmid origin of replication"
     protein_bind    complement(9617..9664)
                     /label=FRT
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="FLP-mediated recombination occurs in the 8-bp core 
                     sequence TCTAGAAA (Turan and Bode, 2011)."
     primer_bind     10037..10055
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
ORIGIN
        1 tcgcgcgttt cggtgatgac ggtgaaaacc tctgacacat gcagctcccg gagacggtca
       61 cagcttgtct gtaagcggat gccgggagca gacaagcccg tcagggcgcg tcagcgggtg
      121 ttggcgggtg tcggggctgg cttaactatg cggcatcaga gcagattgta ctgagagtgc
      181 accataccac cttttcaatt catcattttt tttttattct tttttttgat ttcggtttcc
      241 ttgaaatttt tttgattcgg taatctccga acagaaggaa gaacgaagga aggagcacag
      301 acttagattg gtatatatac gcatatgtag tgttgaagaa acatgaaatt gcccagtatt
      361 cttaacccaa ctgcacagaa caaaaacctg caggaaacga agataaatta gatccaatct
      421 gagcctcgcg gtgatgattg ccagacgggc gggatgctgt ggcgacttgg tggtttttgt
      481 tcgggctgat cgctttcagg gcgacgtttc acggtcttgt cgggatcaga tggacattga
      541 ggtggcctag cgtcagggtg ccgctgagcg ctgacctcaa ctgacgccgt ccaaggccga
      601 cccctgcgct tgacccaaca ccctgacacc aagaagccag tggaacccag gaggtctgcc
      661 aaggctttga aagcttggca cgagcgtctc agctctttga agcctgagtt ttcaggcaag
      721 aattctgctt gtaattgccg ggccggccga caacgcgggg cggcgggagg ggacgctcgg
      781 ccgccggagc tcgcttccat gcattttgga tggagtaaaa attgttaaac acccgacccg
      841 actccgttct ccctctcgtc tcaggtcttt gttgtgcatc catcgccgtg tagcagattt
      901 ccatcgttcg tttgttcgga ctttaccttg tacttttagc ttcattatca tcctacaatc
      961 atccaggatt tcatcttcca caaccgatac gttatcattt ttacttttgt tgccatgttc
     1021 ttcttgagcc ttttctgtct agaggagctc tccctgccgg ctgggagctt gcccaaagct
     1081 ccaagctcta tctgtggcag agctctgaga gcttccattc ccgggatttc ttggcatcag
     1141 attcaattcg aatgtccttt tttccatcca cgctcgagat gaggggtgag gtttgttagt
     1201 ttgacaatac caccaccgcc ttttggtccc taccccagac atggccatca aggtcgttaa
     1261 tgccgtccat ccctcttgtt cggcttgggg tatcgtccat ggctccgcaa tgtcgtcaat
     1321 ggctgcattg acagaggggg actccaaatg aaagaggcaa tcgcaacgct atgaagaccc
     1381 aacaagccat atgatggagc catgggtcta cggtcctttg gcccggatgt ctttggatag
     1441 ctcttgccag cgctttagct gccaactata cacacaccgg aattatacga tttaggtgac
     1501 tgcaggcgag atcgagcaac ccagaaagaa ggggaacctt ttggcggcgg caacctggaa
     1561 tgcatagcat tggatcccat tgagttaggt ttgcaatatc atgatctgta cgtaggaaga
     1621 aggtatctgt gtagcagtgt tgccccccag ttcaagagta gccgagtatg tataaacaaa
     1681 cctcaaactt ctcccctctt ctatagcgtt actgcccaat tacctacctc tacctatcct
     1741 atccaaagaa accggcggca ctacctacct accactaccg acttaccctc tcgctcccgc
     1801 tttcagactt ccttcagtca tcctgtttca gcttcacttc agactgtcaa cgtagcgtag
     1861 gtagtctttt gtctgtccta ccgtgccctt cccgttcgca gccgacttca tctcgcagcg
     1921 agcccactta cacgcgcctc cccttgttca cgaggacctc gcgttactca gattctgact
     1981 cttcgggttg ggtctagtat agtgagattc agttccagat ctgcctctgt tgctgcccga
     2041 aggcactgtt ttccagttcc gtttgtgcct ccctgcccta gaggtggaac tcttcgaggt
     2101 acgttccggt ttgtgagaca cacacttttt ctcttcctgc gacaccgcag cacctgtgtc
     2161 gagccgtcga gccggtggta ttcgcagccc agactcccaa gcgaacctag ctcaagagat
     2221 cgaagccgtg cgccgggaag aggttggaaa ctcggttcag gttgaggttc cagtagcttc
     2281 agtgaggccc cgttccccgc ttccggccgg gcttttaaag cttcgcatcc ccagctgcac
     2341 ccctgaagct tcccacccag accccattcc gggtgacatt cctcgaagct ctaccccacc
     2401 agtgcgtgtt gtctcgtcct ggctacgtcg acctgatctg cttcaaatag aacccaaccg
     2461 tctgctggtc cctcttcttg ctacgtctac tcgccctctc ttcctctcta cgaacctgaa
     2521 aaatcagtgt tctgtggcag cttctggtta cctactaaca cgaagctcgg acgacagaag
     2581 catcttgact tcggcgcgcc cgtcacccat tcatattgag ccacaatgga ggacgccaag
     2641 aacatcaaga agggccccgc ccccttctac cccctcgagg acggcaccgc cggcgagcag
     2701 ctccacaagg ccatgaagcg ctacgccctc gtccccggca ccatcgcctt cgtgggtttc
     2761 ctccagctct cgcctccagc acctgaggca catctcgggc atcttcacaa caacagacac
     2821 tgacatctca ttctcacaga ccgacgccca catcgaggtc aacatcacct acgccgagta
     2881 cttcgagatg tccgtccgcc tcgccgaggc catgaagcgc tacggcctca acaccaacca
     2941 ccgcatcgtc gtctgctccg agaactccct ccagttcttc atgcccgtcc tcggcgccct
     3001 cttcatcggc gtcgccgtcg cccccgccaa cgacatctac aacgagcgcg agctcctcaa
     3061 ctccatgaac atctcccagc ccaccgtcgt cttcgtctcc aagaagggcc tccagaagat
     3121 cctcaacgtc cagaagaagc tccccatcat ccagaagatc atcatcatgg actccaagac
     3181 cgactaccag ggcttccagt ccatgtacac cttcgtcacc tcccacctcc cccccggctt
     3241 caacgagtac gacttcgtcc ccgagtcctt cgaccgcgac aagaccatcg ccctcatcat
     3301 gaactcctcc ggctccaccg gcctccccaa gggcgtcgcc ctcccccacc gcaccgcctg
     3361 cgtccgcttc tcccacgccc gcgaccccat cttcggcaac cagatcatcc ccgacaccgc
     3421 catcctctcc gtcgtcccct tccaccacgg cttcggcatg ttcaccaccc tcggctacct
     3481 catctgcggc ttccgcgtcg tcctcatgta ccgcttcgag gaggagctct tcctccgctc
     3541 cctccaggac tacaagatcc agtccgccct cctcgtcccc accctcttct ccttcttcgc
     3601 caagtccacc ctcatcgaca agtacgacct ctccaacctc cacgagatcg cctccggcgg
     3661 cgcccccctc tccaaggagg tcggcgaggc cgtcgccaag cgcttccacc tccccggcat
     3721 ccgccagggc tacggcctca ccgagaccac ctccgccatc ctcatcaccc ccgagggcga
     3781 cgacaagccc ggcgccgtcg gcaaggtcgt ccccttcttc gaggccaagg tcgtcgacct
     3841 cgacaccggc aagaccctcg gcgtcaacca gcgcggcgag ctctgcgtcc gcggccccat
     3901 gatcatgtcc ggctacgtca acaaccccga ggccaccaac gccctcatcg acaaggacgg
     3961 ctggctccac tccggcgaca tcgcctactg ggacgaggac gagcacttct tcatcgtcga
     4021 ccgcctcaag tccctcctca agtacaaggg ctaccaggtc gcccccgccg agctcgagtc
     4081 catcctcctc cagcacccca acatcttcga cgccggcgtc gccggcctcc ccgacgacga
     4141 cgccggcgag ctccccgccg ccgtcgtcgt cctcgagcac ggcaagacca tgaccgagaa
     4201 ggagatcgtc gactacgtcg cctcccaggt caccaccgcc aagaagctcc gcggcggcgt
     4261 cgtcttcgtc gacgaggtcc ccaagggcct caccggcaag ctcgacgccc gcaagatccg
     4321 cgagatcctc atcaaggcca agaagggcgg caagtccaag ctcctagagg atccgacact
     4381 atagaatacc tattctcggt actatgcatt acatgttaat tattacatgc ttaacgtaat
     4441 tcaacagaaa ttatatgata atcatcgcaa gaccggcaac aggattcaat cttaagaaac
     4501 tttattgcca aatgtttgaa cgatctgcag gtcgacggat cagatctcgg tgacgggcag
     4561 gaccggacgg ggcggtaccg gcaggctgaa gtccagctgc cagaaaccca cgtcatgcca
     4621 gttcccgtgc ttgaagccgg ccgcccgcag catgccgcgg ggggcatatc cgagcgcctc
     4681 gtgcatgcgc acgctcgggt cgttgggcag cccgatgaca gcgaccacgc tcttgaagcc
     4741 ctgtgcctcc agggacttca gcaggtgggt gtagagcgtg gagcccagtc ccgtccgctg
     4801 gtggcggggg gagacgtaca cggtcgactc ggccgtccag tcgtaggcgt tgcgtgcctt
     4861 ccaggggccc gcgtaggcga tgccggcgac ctcgccgtcc acctcggcga cgagccaggg
     4921 atagcgctcc cgcagacgga cgaggtcgtc cgtccactcc tgcggttcct gcggctcggt
     4981 acggaagttg accgtgcttg tctcgatgta gtggttgacg atggtgcaga ccgccggcat
     5041 gtccgcctcg gtggcacggc ggatgtcggc cgggcgtcgt tctgggctca tggttacttc
     5101 ctaatcgaag ctttgcttgg gtagaatagg taagtcagat tgaatctgaa ataaagggag
     5161 gaagggcgaa cttaagaagg tatgaccggg tcgttcactt accttgcttg acaaacgcac
     5221 caagttatcg tgcaccaagc agcagatgat aataatgtcc tcgttcctgt ctgctaataa
     5281 gagtcacact tcgagcgccg ccgctactgc tacaagtggg gctgatctga ccagttgcct
     5341 aaatgaacca tcttgtcaaa cgacacaaat tttgtgctca ccgcctggac gactaaacca
     5401 aaataggcat tcattgttga cctccactag ctccagccaa gcccaaaaag tgctccttca
     5461 atatcatctt ctgtcgagtc tagatgactt gttccgtacc tcgtcaccat ccagccaccg
     5521 tctgaaatag acgcagttgc tggggctgac aggtccgcct tcctcgcaca tatcatagac
     5581 agggcaccgc ccacaaggcg cctccataag gccacttgca ctcggctcaa ctcccataaa
     5641 tgaggtaaca atgcctcctt tggttggatc atggtcgagg cgtcgttgat tgcacgtcct
     5701 cggatcctgt ttggggatgc gcgtcacacg gtaccccttg cgcccattga ccttgacagg
     5761 ctctactaag ccatcgtagt atagcacatc caccaactgc tggatatccg cctcgctcaa
     5821 cgtcgtatta ttcgtgatgc cggcgtggtg gatgaacttt gcaatgtccg acacagtcgg
     5881 gtacgacttg taaccggccg ggagaggcag caattgcggc ttgccgggcc tggtcttctt
     5941 ggccggtgcc ggtgccgccg tgtcgtcgtt ggaaatctcg cccgcgctgc gcttcgtccc
     6001 cttggaaata tcctccgagt ggggaatggt cccctgaacc acgcccttct tgggattctt
     6061 ttcacgctgc tgtgctcctc ctccttgtgc tcctactcct ggtactccag caccgccgcc
     6121 gtgggaagta tatgtcgatt tctgcttgat aaagtcaaac acaatttcct gtagctcgtt
     6181 gatgaaggcc gtgtcgagct cgccctcagt aaaccagggg ccacctgtgg cgcgatcgga
     6241 gggacgtagg ttcgccttga tgtacatctt cttgttgggg tgctcgacgt tcttcatgct
     6301 ggcgatgtag cccttttgct cgagatactt gatgcaatgc ttcataagcg agtcggccat
     6361 gttgagcttg ttcttgatgg tccggttcca gatgccgtcg gccccggctt cgtcgaccat
     6421 ggcgtatacc atcattattt cggagttggg gagggaggtg taccttttgg gaggaagggg
     6481 aggttagttg gtgtgctcat ttaaatgctg tttcctgtgt gaaattgtta tccgctcaca
     6541 attccacaca acatacgagc cggaagcata aagtgtaaag cctggggtgc ctaatgagtg
     6601 agctaactca cattaattgc gttgcgctca ctgcccgctt tccagtcggg aaacctgtcg
     6661 tgccagctgc attaatgaat cggccaacgc gcggggagag gcggtttgcg tattgggcgc
     6721 tcttccgctt cctcgctcac tgactcgctg cgctcggtcg ttcggctgcg gcgagcggta
     6781 tcagctcact caaaggcggt aatacggtta tccacagaat caggggataa cgcaggaaag
     6841 aacatgtgag caaaaggcca gcaaaaggcc aggaaccgta aaaaggccgc gttgctggcg
     6901 tttttccata ggctccgccc ccctgacgag catcacaaaa atcgacgctc aagtcagagg
     6961 tggcgaaacc cgacaggact ataaagatac caggcgtttc cccctggaag ctccctcgtg
     7021 cgctctcctg ttccgaccct gccgcttacc ggatacctgt ccgcctttct cccttcggga
     7081 agcgtggcgc tttctcatag ctcacgctgt aggtatctca gttcggtgta ggtcgttcgc
     7141 tccaagctgg gctgtgtgca cgaacccccc gttcagcccg accgctgcgc cttatccggt
     7201 aactatcgtc ttgagtccaa cccggtaaga cacgacttat cgccactggc agcagccact
     7261 ggtaacagga ttagcagagc gaggtatgta ggcggtgcta cagagttctt gaagtggtgg
     7321 cctaactacg gctacactag aagaacagta tttggtatct gcgctctgct gaagccagtt
     7381 accttcggaa aaagagttgg tagctcttga tccggcaaac aaaccaccgc tggtagcggt
     7441 ggtttttttg tttgcaagca gcagattacg cgcagaaaaa aaggatctca agaagatcct
     7501 ttgatctttt ctacggggtc tgacgctcag tggaacgaaa actcacgtta agggattttg
     7561 gtcatgagat tatcaaaaag gatcttcacc tagatccttt taaattaaaa atgaagtttt
     7621 aaatcaatct aaagtatata tgagtaaact tggtctgaca gttaccaatg cttaatcagt
     7681 gaggcaccta tctcagcgat ctgtctattt cgttcatcca tagttgcctg actccccgtc
     7741 gtgtagataa ctacgatacg ggagggctta ccatctggcc ccagtgctgc aatgataccg
     7801 cgagacccac gctcaccggc tccagattta tcagcaataa accagccagc cggaagggcc
     7861 gagcgcagaa gtggtcctgc aactttatcc gcctccatcc agtctattaa ttgttgccgg
     7921 gaagctagag taagtagttc gccagttaat agtttgcgca acgttgttgc cattgctaca
     7981 ggcatcgtgg tgtcacgctc gtcgtttggt atggcttcat tcagctccgg ttcccaacga
     8041 tcaaggcgag ttacatgatc ccccatgttg tgcaaaaaag cggttagctc cttcggtcct
     8101 ccgatcgttg tcagaagtaa gttggccgca gtgttatcac tcatggttat ggcagcactg
     8161 cataattctc ttactgtcat gccatccgta agatgctttt ctgtgactgg tgagtactca
     8221 accaagtcat tctgagaata gtgtatgcgg cgaccgagtt gctcttgccc ggcgtcaata
     8281 cgggataata ccgcgccaca tagcagaact ttaaaagtgc tcatcattgg aaaacgttct
     8341 tcggggcgaa aactctcaag gatcttaccg ctgttgagat ccagttcgat gtaacccact
     8401 cgtgcaccca actgatcttc agcatctttt actttcacca gcgtttctgg gtgagcaaaa
     8461 acaggaaggc aaaatgccgc aaaaaaggga ataagggcga cacggaaatg ttgaatactc
     8521 atactcttcc tttttcaata ttattgaagc atttatcagg gttattgtct catgagcgga
     8581 tacatatttg aatgtattta gaaaaataaa caaatagggg ttccgcgcac atttccccga
     8641 aaagtgccac ctgaacgaag catctgtgct tcattttgta gaacaaaaat gcaacgcgag
     8701 agcgctaatt tttcaaacaa agaatctgag ctgcattttt acagaacaga aatgcaacgc
     8761 gaaagcgcta ttttaccaac gaagaatctg tgcttcattt ttgtaaaaca aaaatgcaac
     8821 gcgagagcgc taatttttca aacaaagaat ctgagctgca tttttacaga acagaaatgc
     8881 aacgcgagag cgctatttta ccaacaaaga atctatactt cttttttgtt ctacaaaaat
     8941 gcatcccgag agcgctattt ttctaacaaa gcatcttaga ttactttttt tctcctttgt
     9001 gcgctctata atgcagtctc ttgataactt tttgcactgt aggtccgtta aggttagaag
     9061 aaggctactt tggtgtctat tttctcttcc ataaaaaaag cctgactcca cttcccgcgt
     9121 ttactgatta ctagcgaagc tgcgggtgca ttttttcaag ataaaggcat ccccgattat
     9181 attctatacc gatgtggatt gcgcatactt tgtgaacaga aagtgatagc gttgatgatt
     9241 cttcattggt cagaaaatta tgaacggttt cttctatttt gtctctatat actacgtata
     9301 ggaaatgttt acattttcgt attgttttcg attcactcta tgaatagttc ttactacaat
     9361 ttttttgtct aaagagtaat actagagata aacataaaaa atgtagaggt cgagtttaga
     9421 tgcaagttca aggagcgaaa ggtggatggg taggttatat agggatatag cacagagata
     9481 tatagcaaag agatactttt gagcaatgtt tgtggaagcg gtattcgcaa tattttagta
     9541 gctcgttaca gtccggtgcg tttttggttt tttgaaagtg cgtcttcaga gcgcttttgg
     9601 ttttcaaaag cgctctgaag ttcctatact ttctagagaa taggaacttc ggaataggaa
     9661 cttcaaagcg tttccgaaaa cgagcgcttc cgaaaatgca acgcgagctg cgcacataca
     9721 gctcactgtt cacgtcgcac ctatatctgc gtgttgcctg tatatatata tacatgagaa
     9781 gaacggcata gtgcgtgttt atgcttaaat gcgtacttat atgcgtctat ttatgtagga
     9841 tgaaaggtag tctagtacct cctgtgatat tatcccattc catgcggggt atcgtatgct
     9901 tccttcagca ctacccttta gctgttctat atgctgccac tcctcaattg gattagtctc
     9961 atccttcaat gctatcattt cctttgatat tggatcatac taagaaacca ttattatcat
    10021 gacattaacc tataaaaata ggcgtatcac gaggcccttt cgtc
//
