LOCUS       sequence-176876-        4900 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  PCR tagging module with yeast codon optimized mTurquoise2.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4900)
  AUTHORS   Burns S, Avena JS, Unruh JR, Yu Z, Smith SE, Slaughter BD, Winey M, 
            Jaspersen SL
  TITLE     Structured illumination with particle averaging reveals novel roles 
            for yeast centrosome components during duplication.
  JOURNAL   Elife. 2015 Sep 15;4. doi: 10.7554/eLife.08586.
  PUBMED    26371506
REFERENCE   2  (bases 1 to 4900)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Elife."; 
            date: "2015-09-15"; volume: "4. doi"; pages: " 10.7554/eLife.08586"
COMMENT     Sequence Label: pSJ1458 (pFA6-link-mTurquoise2-CaURA3MX)
FEATURES             Location/Qualifiers
     source          1..4900
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     68..86
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     complement(124..146)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     246..265
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     promoter        346..364
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     346..363
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     terminator      1171..1358
                     /gene="S. cerevisiae ADH1"
                     /label=ADH1 terminator
                     /note="transcription terminator for the S. cerevisiae 
                     alcohol dehydrogenase 1 (ADH1) gene"
     primer_bind     complement(1226..1250)
                     /label=pHybLex-R
                     /note="ADH terminator, reverse primer"
     promoter        1433..1776
                     /label=TEF promoter
                     /note="Ashbya gossypii TEF promoter"
     terminator      2595..2792
                     /label=TEF terminator
                     /note="Ashbya gossypii TEF terminator"
     primer_bind     complement(2899..2918)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(2900..2918)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(3005..3022)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(3176..3764)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(3256..3275)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(3935..4726,4727..4795))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     4558..4577
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(4796..4900)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 cacatttccc cgaaaagtgc cacctgacgt ctaagaaacc attattatca tgacattaac
       61 ctataaaaat aggcgtatca cgaggccctt tcgtctcgcg cgtttcggtg atgacggtga
      121 aaacctctga cacatgcagc tcccggagac ggtcacagct tgtctgtaag cggatgccgg
      181 gagcagacaa gcccgtcagg gcgcgtcagc gggtgttggc gggtgtcggg gctggcttaa
      241 ctatgcggca tcagagcaga ttgtactgag agtgcaccat atggacatat tgtcgttaga
      301 acgcggctac aattaataca taaccttatg tatcatacac atacgattta ggtgacacta
      361 tagaacgcgg ccgccagctg aagcttcgta cgctgcaggt cgacggatcg gtgacggtgc
      421 tggtttaatt aacatgtcta aaggtgaaga attgtttact ggtgttgttc caattttggt
      481 tgaattggat ggtgatgtta atggtcataa attttctgtt tctggtgaag gtgaaggtga
      541 tgctacttat ggtaaattga ctttgaaatt tatttgtact actggtaaat tgccagttcc
      601 atggccaact ttggttacta ctttgtcttg gggtgttcaa tgttttgcta gatatccaga
      661 tcatatgaaa caacatgatt tttttaaatc tgctatgcca gaaggttatg ttcaagaaag
      721 aactattttt tttaaagatg atggtaatta taaaactaga gctgaagtta aatttgaagg
      781 tgatactttg gttaatagaa ttgaattgaa aggtattgat tttaaagaag atggtaatat
      841 tttgggtcat aaattggaat ataattattt ttctgataat gtttatatta ctgctgataa
      901 acaaaaaaat ggtattaaag ctaattttaa aattagacat aatattgaag atggtggtgt
      961 tcaattggct gatcattatc aacaaaatac tccaattggt gatggtccag ttttgttgcc
     1021 agataatcat tatttgtcta ctcaatctgc tttgtctaaa gatccaaatg aaaaaagaga
     1081 tcatatggtt ttgttggaat ttgttactgc tgctggtatt actttgggta tggatgaatt
     1141 gtataaataa ggcgcgccac ttctaaataa gcgaatttct tatgatttat gatttttatt
     1201 attaaataag ttataaaaaa aataagtgta tacaaatttt aaagtgactc ttaggtttta
     1261 aaacgaaaat tcttattctt gagtaactct ttcctgtagg tcaggttgct ttctcaggta
     1321 tagtatgagg tcgctcttat tgaccacacc tctaccggca gatccgctag ggataacagg
     1381 gtaatataga tctgtttagc ttgcctcgtc cccgccgggt cacccggcca gcgacatgga
     1441 ggcccagaat accctccttg acagtcttga cgtgcgcagc tcaggggcat gatgtgactg
     1501 tcgcccgtac atttagccca tacatcccca tgtataatca tttgcatcca tacattttga
     1561 tggccgcacg gcgcgaagca aaaattacgg ctcctcgctg cggacctgcg agcagggaaa
     1621 cgctcccctc acagacgcgt tgaattgtcc ccacgccgcg cccctgtaga gaaatataaa
     1681 aggttaggat ttgccactga ggttcttctt tcatatactt ccttttaaaa tcttgctagg
     1741 atacagttct cacatcacat ccgaacataa acaaccatga cagtcaacac taagacctat
     1801 agtgagagag cagaaactca tgcctcacca gtagcacaac gattatttcg attaatggaa
     1861 ctgaagaaaa ccaatttatg tgcatcaatt gatgttgata ccactaagga attccttgaa
     1921 ttaattgata aattgggtcc ttatgtatgc ttaatcaaga ctcatattga tataatcaat
     1981 gatttttcct atgaatccac tattgaacca ttattagaac tttcacgtaa acatcaattt
     2041 atgatttttg aagatagaaa atttgctgat attggtaata ccgtgaagaa acaatatatt
     2101 ggtggagttt ataaaattag tagttgggca gatattacta atgctcatgg tgtcactggg
     2161 aatggagtag ttgaaggatt aaaacaggga gctaaagaaa ccaccaccaa ccaagagcca
     2221 agagggttat tgatgttagc tgaattatca tcagtgggat cattagcata tggagaatat
     2281 tctcaaaaaa ctgttgaaat tgctaaatcc gataaggaat ttgttattgg atttattgcc
     2341 caacgtgata tgggtggaca agaagaagga tttgattggc ttattatgac acctggagtt
     2401 ggattagatg ataaaggtga tggattagga caacaatata gaactgttga tgaagttgtt
     2461 agcactggaa ctgatattat cattgttggt agaggattgt ttggtaaagg aagagatcca
     2521 gatattgaag gtaaaaggta tagagatgct ggttggaatg cttatttgaa aaagactggc
     2581 caattataat cagtactgac aataaaaaga ttcttgtttt caagaacttg tcatttgtat
     2641 agttttttta tattgtagtt gttctatttt aatcaaatgt tagcgtgatt tatatttttt
     2701 ttcgcctcga catcatctgc ccagatgcga agttaagtgc gcagaaagta atatcatgcg
     2761 tcaatcgtat gtgaatgctg gtcgctatac tgctgtcgat tcgatactaa cgccgccatc
     2821 cagtgtcgaa aacgagctcg aattcatcga tgatatcaga tccactagtg gcctatgcgg
     2881 ccgcggatct gccggtctcc ctatagtgag tcgtattaat ttcgataagc caggttaacc
     2941 tgcattaatg aatcggccaa cgcgcgggga gaggcggttt gcgtattggg cgctcttccg
     3001 cttcctcgct cactgactcg ctgcgctcgg tcgttcggct gcggcgagcg gtatcagctc
     3061 actcaaaggc ggtaatacgg ttatccacag aatcagggga taacgcagga aagaacatgt
     3121 gagcaaaagg ccagcaaaag gccaggaacc gtaaaaaggc cgcgttgctg gcgtttttcc
     3181 ataggctccg cccccctgac gagcatcaca aaaatcgacg ctcaagtcag aggtggcgaa
     3241 acccgacagg actataaaga taccaggcgt ttccccctgg aagctccctc gtgcgctctc
     3301 ctgttccgac cctgccgctt accggatacc tgtccgcctt tctcccttcg ggaagcgtgg
     3361 cgctttctca tagctcacgc tgtaggtatc tcagttcggt gtaggtcgtt cgctccaagc
     3421 tgggctgtgt gcacgaaccc cccgttcagc ccgaccgctg cgccttatcc ggtaactatc
     3481 gtcttgagtc caacccggta agacacgact tatcgccact ggcagcagcc actggtaaca
     3541 ggattagcag agcgaggtat gtaggcggtg ctacagagtt cttgaagtgg tggcctaact
     3601 acggctacac tagaagaaca gtatttggta tctgcgctct gctgaagcca gttaccttcg
     3661 gaaaaagagt tggtagctct tgatccggca aacaaaccac cgctggtagc ggtggttttt
     3721 ttgtttgcaa gcagcagatt acgcgcagaa aaaaaggatc tcaagaagat cctttgatct
     3781 tttctacggg gtctgacgct cagtggaacg aaaactcacg ttaagggatt ttggtcatga
     3841 gattatcaaa aaggatcttc acctagatcc ttttaaatta aaaatgaagt tttaaatcaa
     3901 tctaaagtat atatgagtaa acttggtctg acagttacca atgcttaatc agtgaggcac
     3961 ctatctcagc gatctgtcta tttcgttcat ccatagttgc ctgactcccc gtcgtgtaga
     4021 taactacgat acgggagggc ttaccatctg gccccagtgc tgcaatgata ccgcgagacc
     4081 cacgctcacc ggctccagat ttatcagcaa taaaccagcc agccggaagg gccgagcgca
     4141 gaagtggtcc tgcaacttta tccgcctcca tccagtctat taattgttgc cgggaagcta
     4201 gagtaagtag ttcgccagtt aatagtttgc gcaacgttgt tgccattgct acaggcatcg
     4261 tggtgtcacg ctcgtcgttt ggtatggctt cattcagctc cggttcccaa cgatcaaggc
     4321 gagttacatg atcccccatg ttgtgcaaaa aagcggttag ctccttcggt cctccgatcg
     4381 ttgtcagaag taagttggcc gcagtgttat cactcatggt tatggcagca ctgcataatt
     4441 ctcttactgt catgccatcc gtaagatgct tttctgtgac tggtgagtac tcaaccaagt
     4501 cattctgaga atagtgtatg cggcgaccga gttgctcttg cccggcgtca atacgggata
     4561 ataccgcgcc acatagcaga actttaaaag tgctcatcat tggaaaacgt tcttcggggc
     4621 gaaaactctc aaggatctta ccgctgttga gatccagttc gatgtaaccc actcgtgcac
     4681 ccaactgatc ttcagcatct tttactttca ccagcgtttc tgggtgagca aaaacaggaa
     4741 ggcaaaatgc cgcaaaaaag ggaataaggg cgacacggaa atgttgaata ctcatactct
     4801 tcctttttca atattattga agcatttatc agggttattg tctcatgagc ggatacatat
     4861 ttgaatgtat ttagaaaaat aaacaaatag gggttccgcg
//
