LOCUS       sequence-176914-        2236 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 2236)
  TITLE     Additional Tabin lab plasmids
REFERENCE   2  (bases 1 to 2236)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pBS cHoxD9 (CT#674)
FEATURES             Location/Qualifiers
     source          1..2236
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     85..104
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(323..427)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      complement(453..908)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(590..611)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     802..821
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     1035..1057
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     1049..1066
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     1050..1066
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     1073..1092
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        1073..1091
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     1116..1132
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     primer_bind     1117..1133
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
ORIGIN
        1 ctgtgactgg tgagtactca accaagtcat tctgagaata gtgtatgcgg cgaccgagtt
       61 gctcttgccc ggcgtcaata cgggataata ccgcgccaca tagcagaact ttaaaagtgc
      121 tcatcattgg aaaacgttct tcggggcgaa aactctcaag gatcttaccg ctgttgagat
      181 ccagttcgat gtaacccact cgtgcaccca actgatcttc agcatctttt actttcacca
      241 gcgtttctgg gtgagcaaaa acaggaaggc aaaatgccgc aaaaaaggga ataagggcga
      301 cacggaaatg ttgaatactc atactcttcc tttttcaata ttattgaagc atttatcagg
      361 gttattgtct catgagcgga tacatatttg aatgtattta gaaaaataaa caaatagggg
      421 ttccgcgcac atttccccga aaagtgccac ctaaattgta agcgttaata ttttgttaaa
      481 attcgcgtta aatttttgtt aaatcagctc attttttaac caataggccg aaatcggcaa
      541 aatcccttat aaatcaaaag aatagaccga gatagggttg agtgttgttc cagtttggaa
      601 caagagtcca ctattaaaga acgtggactc caacgtcaaa gggcgaaaaa ccgtctatca
      661 gggcgatggc ccactacgtg aaccatcacc ctaatcaagt tttttggggt cgaggtgccg
      721 taaagcacta aatcggaacc ctaaagggag cccccgattt agagcttgac ggggaaagcc
      781 ggcgaacgtg gcgagaaagg aagggaagaa agcgaaagga gcgggcgcta gggcgctggc
      841 aagtgtagcg gtcacgctgc gcgtaaccac cacacccgcc gcgcttaatg cgccgctaca
      901 gggcgcgtcc cattcgccat tcaggctgcg caactgttgg gaagggcgat cggtgcgggc
      961 ctcttcgcta ttacgccagc tggcgaaagg gggatgtgct gcaaggcgat taagttgggt
     1021 aacgccaggg ttttcccagt cacgacgttg taaaacgacg gccagtgaat tgtaatacga
     1081 ctcactatag ggcgaattgg gtaccgggcc ccccctcgag gtcgacggta tcgataagct
     1141 tgatatcgaa ttcgcggccg caatgtcgtc tagtggcacc ataagtaact attatgtcga
     1201 ttctctcata ggccatgaaa gcgaagaagt ttacgggagt agattcgtcc aggggggtca
     1261 cagtgcgacc tccaggccat caggtgtggc agagagttcc gatttttcct cctgtagctt
     1321 tgcaccaaaa tccaccgtgt tctccacctc ctggtccacg gttcacccgc agccgcccgc
     1381 ggccatgacc gggatctacc acccctacat gcaccagccc cacttagggg cagccgacgc
     1441 cggccgctac gtgcgctcct ggatcgagcc cttcccgggc ttcccgggcg gcggcggcgg
     1501 cagcggcggc ggcggcggcg gcggctccgc gtccggctcg ggctcgggct cgggctcctc
     1561 cgccgggcgc cactacggga taaagccgga gacgggagca gccgcctcct cttcttcctc
     1621 ctcctcctcc tcctcctcca aaaggactga gtgctcctcg tcccgagagt cccaggggat
     1681 cgccgtgccc gaatacacgt gcggcgcttt cctgccggag ccccgagaga aggcggccgc
     1741 cggcagcgcc aaggagcccg ccgcctgcag ccacagcccc ggccccggcg gagagccgaa
     1801 ggaggagaag cagcagcaac ttgacccaaa taaccccgcg gcaaactgga tccacgctcg
     1861 ttcgacgagg aaaaagcgtt gcccctacac taagtatcaa actctggaac tggagaagga
     1921 gtttctcttt aacatgtacc tcacccgcga ccgccgctac gaagtagcta ggattctaaa
     1981 tctcaccgag agacaggtca aaatctggtt tcagaacaga cggatgaaaa tgaaaaagat
     2041 gaacaaggag aaaggcaata aaggagacta acgccggggt gggaacgcgg ccggggagcg
     2101 cagctctggg gctgaggttg gggctgtggt tttttctttt tcttttatta ttatttgtat
     2161 ttttattttc ggttcgctcg ggttttatgc ttcccctgcc ccctcggccc cgcgtgcgtc
     2221 tctcctcccc cccccc
//
