LOCUS       sequence-176922-        5420 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  FRET acceptor for TrkB mEGFP.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 5420)
  AUTHORS   Harward SC, Hedrick NG, Hall CE, Parra-Bueno P, Milner TA, Pan E, 
            Laviv T, Hempstead BL, Yasuda R, McNamara JO
  TITLE     Autocrine BDNF-TrkB signalling within a single dendritic spine.
  JOURNAL   Nature. 2016 Sep 28;538(7623):99-103. doi: 10.1038/nature19766.
  PUBMED    27680698
REFERENCE   2  (bases 1 to 5420)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10"; journalName: 
            "Nature"; date: "2016-09-28- 28"; volume: "538"; issue: "7623"; 
            pages: "99-103"
COMMENT     Sequence Label: TrkB Acceptor
FEATURES             Location/Qualifiers
     source          1..5420
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     38..60
                     /label=pCAGGS-5
                     /note="Chimeric intron in CAG promoter, forward primer"
     primer_bind     126..145
                     /label=pCAG-F
                     /note="Rabbit beta-globin intron, for pCAG plasmids, 
                     forward primer"
     regulatory      179..188
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             185..859
                     /codon_start=1
                     /product="monomeric derivative of DsRed (Campbell et al., 
                     2002)"
                     /label=mRFP1
                     /translation="MASSEDVIKEFMRFKVRMEGSVNGHEFEIEGEGEGRPYEGTQTAK
                     LKVTKGGPLPFAWDILSPQFQYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNFEDGGV
                     VTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASTERMYPEDGALKGEIKM
                     RLKLKDGGHYDAEVKTTYMAKKPVQLPGAYKTDIKLDITSHNEDYTIVEQYERAEGRHS
                     TGA"
     CDS             185..859
                     /codon_start=1
                     /product="monomeric derivative of DsRed (Campbell et al., 
                     2002)"
                     /label=mRFP1
                     /note="mammalian codon-optimized"
                     /translation="MASSEDVIKEFMRFKVRMEGSVNGHEFEIEGEGEGRPYEGTQTAK
                     LKVTKGGPLPFAWDILSPQFQYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNFEDGGV
                     VTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASTERMYPEDGALKGEIKM
                     RLKLKDGGHYDAEVKTTYMAKKPVQLPGAYKTDIKLDITSHNEDYTIVEQYERAEGRHS
                     TGA"
     CDS             1229..1906
                     /codon_start=1
                     /product="monomeric derivative of DsRed (Campbell et al., 
                     2002)"
                     /label=mRFP1
                     /translation="MASSEDVIKEFMRFKVRMEGSVNGHEFEIEGEGEGRPYEGTQTAK
                     LKVTKGGPLPFAWDILSPQFQYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNFEDGGV
                     VTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASTERMYPEDGALKGEIKM
                     RLKLKDGGHYDAEVKTTYMAKKPVQLPGAYKTDIKLDITSHNEDYTIVEQYERAEGRHS
                     TGA"
     CDS             1229..1906
                     /codon_start=1
                     /product="monomeric derivative of DsRed (Campbell et al., 
                     2002)"
                     /label=mRFP1
                     /note="mammalian codon-optimized"
                     /translation="MASSEDVIKEFMRFKVRMEGSVNGHEFEIEGEGEGRPYEGTQTAK
                     LKVTKGGPLPFAWDILSPQFQYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNFEDGGV
                     VTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASTERMYPEDGALKGEIKM
                     RLKLKDGGHYDAEVKTTYMAKKPVQLPGAYKTDIKLDITSHNEDYTIVEQYERAEGRHS
                     TGA"
     primer_bind     complement(1933..1952)
                     /label=Bglob-pA-R
                     /note="Rabbit beta-globin polyA region, reverse primer"
     polyA_signal    1998..2053
                     /label=beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal (Gil and 
                     Proudfoot, 1987)"
     primer_bind     complement(2052..2071)
                     /label=rbglobpA-R
                     /note="Rabbit beta-globin polyA, reverse primer. Also 
                     called rb-glob-pA-term-R"
     primer_bind     complement(2414..2430)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(2414..2430)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(2427..2449)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    2438..2454
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(2462..2468,2469..2486,2487..2492))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    2507..2528
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        2587..2782
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     rep_origin      2633..2768
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     polyA_signal    2788..2922
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(2825..2844)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     2879..2898
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(2990..3007)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(3161..3749)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(3241..3260)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(3920..4711,4712..4780))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     4543..4562
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(4781..4885)
                     /gene="bla"
                     /label=AmpR promoter
     enhancer        4920..5299
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
ORIGIN
        1 ggggacggct gccttcgggg gggacggggc agggcggggt tcggcttctg gcgtgtgacc
       61 ggcggctcta gctagagcct ctgctaacca tgttcatgcc ttcttctttt tcctacagct
      121 cctgggcaac gtgctggtta ttgtgctgtc tcatcatttt ggcaaagaat tcgctagcgc
      181 caccatggcc tcctccgagg acgtcatcaa ggagttcatg cgcttcaagg tgcgcatgga
      241 gggctccgtg aacggccacg agttcgagat cgagggcgag ggcgagggcc gcccctacga
      301 gggcacccag accgccaagc tgaaggtgac caagggcggc cccctgccct tcgcctggga
      361 catcctgtcc cctcagttcc agtacggctc caaggcctac gtgaagcacc ccgccgacat
      421 ccccgactac ttgaagctgt ccttccccga gggcttcaag tgggagcgcg tgatgaactt
      481 cgaggacggc ggcgtggtga ccgtgaccca ggactcctcc ctgcaggacg gcgagttcat
      541 ctacaaggtg aagctgcgcg gcaccaactt cccctccgac ggccccgtaa tgcagaagaa
      601 gaccatgggc tgggaggcct ccaccgagcg gatgtacccc gaggacggcg ccctgaaggg
      661 cgagatcaag atgaggctga agctgaagga cggcggccac tacgacgccg aggtcaagac
      721 cacctacatg gccaagaagc ccgtgcagct gcccggcgcc tacaagaccg acatcaagct
      781 ggacatcacc tcccacaacg aggactacac catcgtggaa cagtacgagc gcgccgaggg
      841 ccgccactcc accggcgcca gatctcgagc tcaagcttcg aattctcaga ccaacgccca
      901 cgagagcaaa gagtggtacc acgcgagcct gaccagagca caggctgagc acatgctaat
      961 gcgcgtccct cgtgatgggg ccttcctggt gcggaagcgg aatgaaccca actcatatgc
     1021 catctctttc cgggctgagg gcaagatcaa gcattgccgt gtccagcaag agggccagac
     1081 agtgatgcta gggaactcgg agttcgacag ccttgttgac ctcatcagct actatgagaa
     1141 acacccgcta taccgcaaga tgaagctgcg ctatcccatc aacgaggagg cactggagaa
     1201 gattggcaca gctgagcctg gatccggaat ggcctcctcc gaggacgtca tcaaggagtt
     1261 catgcgcttc aaggtgcgca tggagggctc cgtgaacggc cacgagttcg agatcgaggg
     1321 cgagggcgag ggccgcccct acgagggcac ccagaccgcc aagctgaagg tgaccaaggg
     1381 cggccccctg cccttcgcct gggacatcct gtcccctcag ttccagtacg gctccaaggc
     1441 ctacgtgaag caccccgccg acatccccga ctacttgaag ctgtccttcc ccgagggctt
     1501 caagtgggag cgcgtgatga acttcgagga cggcggcgtg gtgaccgtga cccaggactc
     1561 ctccctgcag gacggcgagt tcatctacaa ggtgaagctg cgcggcacca acttcccctc
     1621 cgacggcccc gtaatgcaga agaagaccat gggctgggag gcctccaccg agcggatgta
     1681 ccccgaggac ggcgccctga agggcgagat caagatgagg ctgaagctga aggacggcgg
     1741 ccactacgac gccgaggtca agaccaccta catggccaag aagcccgtgc agctgcccgg
     1801 cgcctacaag accgacatca agctggacat cacctcccac aacgaggact acaccatcgt
     1861 ggaacagtac gagcgcgccg agggccgcca ctccaccggc gcctaagcgg ccgcgactct
     1921 agagtcgacg gatctttttc cctctgccaa aaattatggg gacatcatga agccccttga
     1981 gcatctgact tctggctaat aaaggaaatt tattttcatt gcaatagtgt gttggaattt
     2041 tttgtgtctc tcactcggaa ggacatatgg gagggcaaat catttaaaac atcagaatga
     2101 gtatttggtt tagagtttgg caacatatgc ccatatgctg gctgccatga acaaaggttg
     2161 gctataaaga ggtcatcagt atatgaaaca gccccctgct gtccattcct tattccatag
     2221 aaaagccttg acttgaggtt agattttttt tatattttgt tttgtgttat ttttttcttt
     2281 aacatcccta aaattttcct tacatgtttt actagccaga tttttcctcc tctcctgact
     2341 actcccagtc atagctgtcc ctcttctctt atggagatcc ctcgacctgc agcccaagct
     2401 tggcgtaatc atggtcatag ctgtttcctg tgtgaaattg ttatccgctc acaattccac
     2461 acaacatacg agccggaagc ataaagtgta aagcctgggg tgcctaatga gtgagctaac
     2521 tcacattaat tgcgttgcgc tcactgcccg ctttccagtc gggaaacctg tcgtgccagc
     2581 ggatacgcat ctcaattagt cagcaaccat agtcccgccc ctaactccgc ccatcccgcc
     2641 cctaactccg cccagttccg cccattctcc gccccatggc tgactaattt tttttattta
     2701 tgcagaggcc gaggccgcct cggcctctga gctattccag aagtagtgag gaggcttttt
     2761 tggaggccta ggcttttgca aaaagctaac ttgtttattg cagcttataa tggttacaaa
     2821 taaagcaata gcatcacaaa tttcacaaat aaagcatttt tttcactgca ttctagttgt
     2881 ggtttgtcca aactcatcaa tgtatcttat catgtctcga tccgctgcat taatgaatcg
     2941 gccaacgcgc ggggagaggc ggtttgcgta ttgggcgctc ttccgcttcc tcgctcactg
     3001 actcgctgcg ctcggtcgtt cggctgcggc gagcggtatc agctcactca aaggcggtaa
     3061 tacggttatc cacagaatca ggggataacg caggaaagaa catgtgagca aaaggccagc
     3121 aaaaggccag gaaccgtaaa aaggccgcgt tgctggcgtt tttccatagg ctccgccccc
     3181 ctgacgagca tcacaaaaat cgacgctcaa gtcagaggtg gcgaaacccg acaggactat
     3241 aaagatacca ggcgtttccc cctggaagct ccctcgtgcg ctctcctgtt ccgaccctgc
     3301 cgcttaccgg atacctgtcc gcctttctcc cttcgggaag cgtggcgctt tctcatagct
     3361 cacgctgtag gtatctcagt tcggtgtagg tcgttcgctc caagctgggc tgtgtgcacg
     3421 aaccccccgt tcagcccgac cgctgcgcct tatccggtaa ctatcgtctt gagtccaacc
     3481 cggtaagaca cgacttatcg ccactggcag cagccactgg taacaggatt agcagagcga
     3541 ggtatgtagg cggtgctaca gagttcttga agtggtggcc taactacggc tacactagaa
     3601 gaacagtatt tggtatctgc gctctgctga agccagttac cttcggaaaa agagttggta
     3661 gctcttgatc cggcaaacaa accaccgctg gtagcggtgg tttttttgtt tgcaagcagc
     3721 agattacgcg cagaaaaaaa ggatctcaag aagatccttt gatcttttct acggggtctg
     3781 acgctcagtg gaacgaaaac tcacgttaag ggattttggt catgagatta tcaaaaagga
     3841 tcttcaccta gatcctttta aattaaaaat gaagttttaa atcaatctaa agtatatatg
     3901 agtaaacttg gtctgacagt taccaatgct taatcagtga ggcacctatc tcagcgatct
     3961 gtctatttcg ttcatccata gttgcctgac tccccgtcgt gtagataact acgatacggg
     4021 agggcttacc atctggcccc agtgctgcaa tgataccgcg agacccacgc tcaccggctc
     4081 cagatttatc agcaataaac cagccagccg gaagggccga gcgcagaagt ggtcctgcaa
     4141 ctttatccgc ctccatccag tctattaatt gttgccggga agctagagta agtagttcgc
     4201 cagttaatag tttgcgcaac gttgttgcca ttgctacagg catcgtggtg tcacgctcgt
     4261 cgtttggtat ggcttcattc agctccggtt cccaacgatc aaggcgagtt acatgatccc
     4321 ccatgttgtg caaaaaagcg gttagctcct tcggtcctcc gatcgttgtc agaagtaagt
     4381 tggccgcagt gttatcactc atggttatgg cagcactgca taattctctt actgtcatgc
     4441 catccgtaag atgcttttct gtgactggtg agtactcaac caagtcattc tgagaatagt
     4501 gtatgcggcg accgagttgc tcttgcccgg cgtcaatacg ggataatacc gcgccacata
     4561 gcagaacttt aaaagtgctc atcattggaa aacgttcttc ggggcgaaaa ctctcaagga
     4621 tcttaccgct gttgagatcc agttcgatgt aacccactcg tgcacccaac tgatcttcag
     4681 catcttttac tttcaccagc gtttctgggt gagcaaaaac aggaaggcaa aatgccgcaa
     4741 aaaagggaat aagggcgaca cggaaatgtt gaatactcat actcttcctt tttcaatatt
     4801 attgaagcat ttatcagggt tattgtctca tgagcggata catatttgaa tgtatttaga
     4861 aaaataaaca aataggggtt ccgcgcacat ttccccgaaa agtgccacct gggtcgatcg
     4921 acattgatta ttgactagtt attaatagta atcaattacg gggtcattag ttcatagccc
     4981 atatatggag ttccgcgtta cataacttac ggtaaatggc ccgcctggct gaccgcccaa
     5041 cgacccccgc ccattgacgt caataatgac gtatgttccc atagtaacgc caatagggac
     5101 tttccattga cgtcaatggg tggagtattt acggtaaact gcccacttgg cagtacatca
     5161 agtgtatcat atgccaagta cgccccctat tgacgtcaat gacggtaaat ggcccgcctg
     5221 gcattatgcc cagtacatga ccttatggga ctttcctact tggcagtaca tctacgtatt
     5281 agtcatcgct attaccatgg tcgaggtgag ccccacgttc tgcttcactc tccccatctc
     5341 ccccccctcc ccacccccaa ttttgtattt atttattttt taattatttt gtgcagcgat
     5401 gggggcgggg gggggggggg
//
