LOCUS       sequence-176923-       12641 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12641)
  AUTHORS   Henderson MJ, Russell AJ, Hird S, Munoz M, Clancy JL, Lehrbach GM, 
            Calanni ST, Jans DA, Sutherland RL, Watts CK
  TITLE     EDD, the human hyperplastic discs protein, has a role in 
            progesterone receptor coactivation and potential involvement in DNA 
            damage response.
  JOURNAL   J Biol Chem. 2002 Jul 19;277(29):26468-78. Epub 2002 May 13.
  PUBMED    12011095
REFERENCE   2  (bases 1 to 12641)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Biol 
            Chem."; date: "2002-07-19"; volume: "277(29)"; pages: "26468-78. 
            Epub 2002 May 13"
COMMENT     Sequence Label: pSG5 EDD
FEATURES             Location/Qualifiers
     source          1..12641
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     19..41
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     33..50
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     34..50
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(87..107)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        195..405
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     promoter        217..413
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     rep_origin      264..399
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     intron          444..1016
                     /label=beta-globin intron
                     /note="intron from rabbit beta-globin gene"
     primer_bind     1024..1043
                     /label=pCAG-F
                     /note="Rabbit beta-globin intron, for pCAG plasmids, 
                     forward primer"
     primer_bind     1071..1090
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        1071..1089
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             1100..1123
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             9578..9595
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     polyA_signal    9697..9818
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(9734..9753)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     9788..9807
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(9852..9869)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(10023..10611)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(10103..10122)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(10782..11573,11574..11642))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     11405..11424
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(11643..11747)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     11815..11833
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     complement(11871..11893)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     11993..12012
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     rep_origin      complement(12079..12534)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(12216..12237)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     12428..12447
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
ORIGIN
        1 gggtaacgcc agggttttcc cagtcacgac gttgtaaaac gacggccagt gaattgtcga
       61 cttctgaggc ggaaagaacc agctgtggaa tgtgtgtcag ttagggtgtg gaaagtcccc
      121 aggctcccca gcaggcagaa gtatgcaaag catgcatctc aattagtcag caaggaaagt
      181 ccccaggctc cccagcaggc agaagtatgc aaagcatgca tctcaattag tcagcaacca
      241 tagtcccgcc cctaactccg cccatcccgc ccctaactcc gcccagttcc gcccattctc
      301 cgccccatgg ctgactaatt ttttttattt atgcagaggc cgaggccgcc tcggcctctg
      361 agctattcca gaagtagtga agaggctttt ttggaggcct aggcttttgc aaaaagctcc
      421 ggatcgatcc tgagaacttc agggtgagtt tggggaccct tgattgttct ttctttttcg
      481 ctattgtaaa attcatgtta tatggagggg gcaaagtttt cagggtgttg tttagaatgg
      541 gaagatgtcc cttgtatcac catggaccct catgataatt ttgtttcttt cactttctac
      601 tctgttgaca accattgtct cctcttattt tcttttcatt ttctgtaact ttttcgttaa
      661 actttagctt gcatttgtaa cgaattttta aattcacttt tgtttatttg tcagattgta
      721 agtactttct ctaatcactt ttttttcaag gcaatcaggg tatattatat tgtacttcag
      781 cacagtttta gagaacaatt gttataatta aatgataagg tagaatattt ctgcatataa
      841 attctggctg gcgtggaaat attcttattg gtagaaacaa ctacatcctg gtcatcatcc
      901 tgcctttctc tttatggtta caatgatata cactgtttga gatgaggata aaatactctg
      961 agtccaaacc gggcccctct gctaaccatg ttcatgcctt cttctttttc ctacagctcc
     1021 tgggcaacgt gctggttatt gtgctgtctc atcattttgg caaagaattg taatacgact
     1081 cactataggg cgaattatgg actacaagga cgacgatgac aagcttccgt ccgaagcgcg
     1141 cggaattcaa aggcgagtgg aggacgagaa ggaaagcacc atgacgtcca tccatttcgt
     1201 ggttcacccg ctgccgggca ccgaggacca gctcaatgac aggttacgag aagtttctga
     1261 gaagctgaac aaatataatt taaacagcca cccccctttg aatgtattgg aacaggctac
     1321 tattaaacag tgtgtggtgg gaccaaatca tgctgccttt cttcttgagg atggtagagt
     1381 ttgcaggatt ggtttttcag tacagccaga cagattggaa ttgggtaaac ctgataataa
     1441 tgatgggtca aagttgaaca gcaactcggg ggcagggagg acgtcaaggc ctggtaggac
     1501 aagcgactct ccatggtttc tctcaggttc tgagactcta ggcaggctgg caggcaacac
     1561 cttaggaagc cgctggagtt ctggagtggg tggaagtggt ggaggatcct ctggtaggtc
     1621 atcagctgga gctcgagatt cccgccggca gactcgagtt attcggacag gacgggatcg
     1681 agggtctggg cttttgggca gtcagcccca gccagttatt ccagcatctg tcattccaga
     1741 ggagctgatt tcacaggccc aagttgtttt acaaggcaaa tccagaagtg tcattattcg
     1801 agaacttcag agaacaaatc ttgatgtgaa ccttgctgta aataatttac ttagccggga
     1861 tgatgaagat ggagatgatg gggatgatac agccagcgaa tcttatttgc ctggagagga
     1921 tcttatgtct ctccttgatg ccgacattca ttctgcccac ccaagtgtca ttattgatgc
     1981 agatgccatg ttttctgaag acattagcta ttttggttac ccttcttttc gtcgttcatc
     2041 actttccagg ctaggctcat ctcgagttct ccttcttccc ttagagagag actctgagct
     2101 gttgcgtgaa cgtgaatccg ttttacgttt acgtgaacga aggtggcttg atggagcctc
     2161 atttgataat gaaaggggtt ctaccagcaa ggaaggagag ccaaacttgg ataagaagaa
     2221 tacacctgtt caaagtccag tatctctagg agaagatttg cagtggtggc ctgataagga
     2281 tggaacaaaa ttcatctgta ttggggctct gtattctgaa cttctggctg tcagcagtaa
     2341 aggagaactt tatcagtgga aatggagtga atctgagcct tacagaaatg cccagaatcc
     2401 ttcattacat catccacgag caacattttt ggggttaacc aatgaaaaga tagtcctcct
     2461 gtctgcaaat agcataagag caactgtagc tacagaaaat aacaaggttg ctacatgggt
     2521 ggatgaaact ttaagttctg tggcttctaa attagagcac actgctcaga cttactctga
     2581 acttcaagga gagcggatag tttctttaca ttgctgtgcc ctttacacct gcgctcagct
     2641 ggaaaacagt ttatattggt ggggtgtagt tccttttagt caaaggagga aaatgttaga
     2701 gaaagctaga gcaaaaaata aaaagcctaa atccagtgct ggtatttctt caatgccgaa
     2761 catcactgtt ggtacccagg tatgcttgag aaataatcct ctttatcatg ctggagcagt
     2821 tgcattttca attagtgctg ggattcctaa agttggtgtc ttaatggagt cagtttggaa
     2881 tatgaatgac agctgtagat ttcaacttag atctcctgaa agcttgaaaa acatggaaaa
     2941 agctagcaaa actactgaag ctaagcctga aagtaagcag gagccagtga aaacagaaat
     3001 gggtcctcca ccatctccag catccacgtg tagtgatgca tcctcaattg ccagcagtgc
     3061 atcaatgcca tacaaacgac gacggtcaac ccctgcacca aaagaagagg aaaaggtgaa
     3121 tgaagagcag tggtctcttc gggaagtggt ttttgtggaa gatgtcaaga atgttcctgt
     3181 tggcaaggtg ctaaaagtag atggtgccta tgttgctgta aaatttccag gaacctccag
     3241 taatactaac tgtcagaaca gctctggtcc agatgctgac ccttcttctc tcctgcagga
     3301 ttgtaggtta cttagaattg atgaattgca ggttgtcaaa actggtggaa caccgaaggt
     3361 tcccgactgt ttccaaagga ctcctaaaaa gctttgtata cctgaaaaaa cagaaatatt
     3421 agcagtgaat gtagattcca aaggtgttca tgctgttctg aagactggaa attgggtacg
     3481 atactgtatc tttgatcttg ctacaggaaa agcagaacag gaaaataatt ttcctacaag
     3541 cagcattgct ttccttggtc agaatgagag gaatgtagcc attttcactg ctggacagga
     3601 atctcccatt attcttcgag atggaaatgg taccatctac ccaatggcca aagattgcat
     3661 gggaggaata agggatcccg attggctgga tcttccacct attagtagtc ttggaatggg
     3721 tgtgcattct ttaataaatc ttcctgccaa ttcaacaatc aaaaagaaag ctgctgttat
     3781 catcatggct gtagagaaac aaaccttaat gcaacacatt ctgcgctgtg actatgaggc
     3841 ctgtcgacaa tatctaatga atcttgagca agcggttgtt ttagagcaga atctacagat
     3901 gctgcagaca ttcatcagcc acagatgtga tggaaatcga aatattttgc atgcttgtgt
     3961 atcagtttgc tttccaacca gcaataaaga aactaaagaa gaagaggaag cggagcgttc
     4021 tgaaagaaat acatttgcag aaaggctttc tgctgttgag gccattgcaa atgcaatatc
     4081 agttgtttca agtaatggcc caggtaatcg ggctggatca tcaagtagcc gaagtttgag
     4141 attacgggaa atgatgagac gttcgttgag agcagctggt ttgggtagac atgaagctgg
     4201 agcttcatcc agtgaccacc aggatccagt ttcacccccc atagctcccc ctagttgggt
     4261 tcctgaccct cctgcgatgg atcctgatgg tgacattgat tttatcctgg cccccgctgt
     4321 gggatctctt accacagcag caaccggtac tggtcaagga ccaagcacct ccactattcc
     4381 aggtccttcc acagagccat ctgtagtaga atccaaggat cgaaaggcga atgctcattt
     4441 tatattgaaa ttgttatgtg acagtgtggt tctccagccc tatctacgag aacttctttc
     4501 tgccaaggat gcaagaggga tgaccccatt tatgtcagct gtaagtggcc gagcttatcc
     4561 tgctgcaatt accatcttag aaactgctca gaaaattgca aaagctgaaa tatcctcaag
     4621 tgaaaaagag gaagatgtat tcatgggaat ggtttgccca tcaggtacca accctgatga
     4681 ctctccttta tatgttttat gttgtaatga cacttgcagt tttacatgga ctggagcaga
     4741 gcacattaac caggatattt ttgagtgtcg aacttgtggc ttgctggagt cactgtgttg
     4801 ttgtacggaa tgtgcaaggg tttgtcataa aggtcatgat tgcaaactca aacggacatc
     4861 accaacagcc tactgtgatt gttgggagaa atgtaaatgt aaaactctta ttgctggaca
     4921 gaaatctgct cgtcttgatc tactttatcg cctgctcact gctactaatc tggttactct
     4981 gccaaacagc aggggagagc acctcttact attcttagta cagacagtcg caaggcagac
     5041 ggtggagcat tgtcaataca ggccacctcg aatcagggaa gatcgtaacc gaaaaacagc
     5101 cagtcctgaa gattcagata tgccagatca tgatttggag cctccaagat ttgcccagct
     5161 tgcattggag cgtgttctac aggactggaa tgccttgaaa tctatgatta tgtttgggtc
     5221 gcaggagaat aaagaccctc ttagtgccag cagtagaata ggccatcttt tgccagaaga
     5281 gcaagtatac ctcaatcagc aaagtggcac aattcggctg gactgtttca ctcattgcct
     5341 tatagttaag tgtacagcag atattttgct tttagatact ctactaggta cactagtgaa
     5401 agaactccaa aacaaatata cacctggacg tagagaagaa gctattgctg tgacaatgag
     5461 gtttctacgt tcagtggcaa gagtttttgt tattctgagt gtggaaatgg cttcatccaa
     5521 aaagaaaaac aactttattc cacagccaat tggaaaatgc aagcgtgtat tccaagcatt
     5581 gctaccttac gctgtggaag aattgtgcaa cgtagcagag tcactgattg ttcctgtcag
     5641 aatggggatt gctcgtccaa ctgcaccatt taccctggct agtactagca tagatgccat
     5701 gcagggcagt gaagaattat tttcagtgga accactacca ccacgaccat catctgatca
     5761 gtctagcagc tccagtcagt ctcagtcatc ctacatcatc aggaatccac agcagaggcg
     5821 catcagccag tcacagcccg ttcggggcag agatgaagaa caggatgata ttgtttcagc
     5881 agatgtggaa gaggttgagg tggtggaggg tgtggctgga gaagaggatc atcatgatga
     5941 acaggaagaa cacggggaag aaaatgctga ggcagaggga caacatgatg agcatgatga
     6001 agacgggagt gatatggagc tggacttgtt agcagcagct gaaacagaaa gtgatagtga
     6061 aagtaaccac agcaaccaag ataatgctag tgggcgcaga agcgttgtca ctgcagcaac
     6121 tgctggttca gaagcaggag caagcagtgt tcctgccttc ttttctgaag atgattctca
     6181 atcgaatgac tcaagtgatt ctgatagcag tagtagtcag agtgacgaca tagaacagga
     6241 gacctttatg cttgatgagc cattagaaag aaccacaaat agctcccatg ccaatggtgc
     6301 tgcccaagct ccccgttcaa tgcagtgggc tgtccgcaac acccagcatc agcgagcagc
     6361 cagtacagcc ccttccagta catctacacc agcagcaagt tcagcgggtt tgatttatat
     6421 tgatccttca aacttacgcc ggagtggtac catcagtaca agtgctgcag ctgcagcagc
     6481 tgctttggaa gctagcaacg ccagcagtta cctaacatct gcaagcagtt tagccagggc
     6541 ttacagcatt gtcattagac aaatctcgga cttgatgggc cttattccta agtataatca
     6601 cctagtatac tctcagattc cagcagctgt gaaattgact taccaagatg cagtaaactt
     6661 acagaactat gtagaagaaa agcttattcc cacttggaac tggatggtca gtattatgga
     6721 ttctactgaa gctcaattac gttatggttc tgcattagca tctgctggtg atcctggaca
     6781 tccaaatcat cctcttcacg cttctcagaa ttcagcgaga agagagagga tgactgcgcg
     6841 agaagaagct agcttacgaa cacttgaagg cagacgacgt gccaccttgc ttagcgcccg
     6901 tcaaggaatg atgtctgcac gaggagactt cctaaattat gctctgtctc taatgcggtc
     6961 tcataatgat gagcattctg atgttcttcc agttttggat gtttgctcat tgaagcatgt
     7021 ggcatatgtt tttcaagcac ttatatactg gattaaggca atgaatcagc agacaacatt
     7081 ggatacacct caactagaac gcaaaaggac gcgagaactc ttggaactgg gtattgataa
     7141 tgaagattca gaacatgaaa atgatgatga caccaatcaa agtgctactt tgaatgataa
     7201 ggatgatgac tctcttcctg cagaaactgg ccaaaaccat ccatttttcc gacgttcaga
     7261 ctccatgaca ttccttgggt gtataccccc aaatccattt gaagtgcctc tggctgaagc
     7321 catccccttg gctgatcagc cacatctgtt gcagccaaat gctagaaagg aggatctttt
     7381 tggccgtcca agtcagggtc tttattcttc atctgccagt agtgggaaat gtttaatgga
     7441 ggttacagtg gatagaaact gcctagaggt tcttccaaca aaaatgtctt atgctgccaa
     7501 tctgaaaaat gtaatgaaca tgcaaaaccg gcaaaaaaaa gaaggggaag aacagcccgt
     7561 gctgccagaa gaaactgaga gttcaaaacc agggccatct gctcatgatc ttgctgcaca
     7621 attaaaaagt agcttactag cagaaatagg acttactgaa agtgaagggc cacctctcac
     7681 atctttcagg ccacagtgta gctttatggg aatggttatt tcccatgata tgctgctagg
     7741 acgttggcgc ctttctttag aactgttcgg cagggtattc atggaagatg ttggagcaga
     7801 acctggatca atcctaactg aattgggtgg ttttgaggta aaagaatcaa aattccgcag
     7861 agaaatggaa aaactgagaa accagcagtc aagagatttg tcactagagg ttgatcggga
     7921 tcgagatctt ctcattcagc agactatgag gcagcttaac aatcactttg gtcgaagatg
     7981 tgctactaca ccaatggctg tacacagagt aaaagtcaca tttaaggatg agccaggaga
     8041 gggcagtggt gtagcacgaa gtttttatac agccattgca caagcatttt tatcaaatga
     8101 aaaattgcca aatctagagt gtatccaaaa tgccaacaaa ggcacccaca caagtttaat
     8161 gcagagatta aggaaccgag gagagagaga ccgggaaagg gagagagaaa gggaaatgag
     8221 gaggagtagt ggtttgcgag caggttctcg gagggaccgg gatagagact ttagaagaca
     8281 gctttccatc gacactaggc cctttagacc agcctctgaa gggaatccta gcgatgatcc
     8341 tgagcctttg ccagcacatc ggcaggcact tggagagagg ctttatcctc gtgtacaagc
     8401 aatgcaacca gcatttgcaa gtaaaatcac tggcatgttg ttggaattat ccccagctca
     8461 gctgcttctc cttctagcaa gtgaggattc tctgagagca agagtggatg aggccatgga
     8521 actcattatt gcacatggac gggaaaatgg agctgatagt atcctggatc ttggattagt
     8581 agactcctca gaaaaggtac agcaggaaaa ccgaaagcgc catggctcta gtcgaagtgt
     8641 agtagatatg gatttagatg atacagatga tggtgatgac aatgcccctt tgttttacca
     8701 acctgggaaa agaggatttt atactccaag gcctggcaag aacacagaag caaggttgaa
     8761 ttgtttcaga aacattggca ggattcttgg actatgtctg ttacagaatg aactctgtcc
     8821 tatcacattg aatagacatg taattaaagt attgcttggt agaaaagtca attggcatga
     8881 ttttgctttt tttgatcctg taatgtatga gagtttgcgg caactaatcc tcgcgtctca
     8941 gagttcagat gctgatgctg ttttctcagc aatggatttg gcatttgcaa ttgacctgtg
     9001 taaagaagaa ggtggaggac aggttgaact cattcctaat ggtgtaaata taccagtcac
     9061 tccacagaat gtatatgagt atgtgcggaa atacgcagaa cacagaatgt tggtagttgc
     9121 agaacagccc ttacatgcaa tgaggaaagg tctactagat gtgcttccaa aaaattcatt
     9181 agaagattta acggcagaag attttaggct tttggtaaat ggctgcggtg aagtcaatgt
     9241 gcaaatgctg atcagtttta cctctttcaa tgatgaatca ggagaaaatg ctgagaagct
     9301 tctgcagttc aagcgttggt tctggtcaat agtagagaag atgagcatga cagaacgaca
     9361 agatcttgtt tacttttgga catcaagccc atcactgcca gccagtgaag aaggattcca
     9421 gcctatgccc tcaatcacaa taagaccacc agatgaccaa catcttccta ctgcaaatac
     9481 ttgcatttct cgactttacg tcccactcta ttcctctaaa cagattctca aacagaaatt
     9541 gttactcgcc attaagacca agaattttgg ttttgtgcat caccatcacc atcactagcc
     9601 cctacgtcga cgagctcact agtcgcggcc gctttcgggt accgagctct gaattcgata
     9661 tcactagtaa ttcggatcca gatcttatta aagcagaact tgtttattgc agcttataat
     9721 ggttacaaat aaagcaatag catcacaaat ttcacaaata aagcattttt ttcactgcat
     9781 tctagttgtg gtttgtccaa actcatcaat gtatcttatc atgtctggtc gactctagac
     9841 tcttccgctt cctcgctcac tgactcgctg cgctcggtcg ttcggctgcg gcgagcggta
     9901 tcagctcact caaaggcggt aatacggtta tccacagaat caggggataa cgcaggaaag
     9961 aacatgtgag caaaaggcca gcaaaaggcc aggaaccgta aaaaggccgc gttgctggcg
    10021 tttttccata ggctccgccc ccctgacgag catcacaaaa atcgacgctc aagtcagagg
    10081 tggcgaaacc cgacaggact ataaagatac caggcgtttc cccctggaag ctccctcgtg
    10141 cgctctcctg ttccgaccct gccgcttacc ggatacctgt ccgcctttct cccttcggga
    10201 agcgtggcgc tttctcatag ctcacgctgt aggtatctca gttcggtgta ggtcgttcgc
    10261 tccaagctgg gctgtgtgca cgaacccccc gttcagcccg accgctgcgc cttatccggt
    10321 aactatcgtc ttgagtccaa cccggtaaga cacgacttat cgccactggc agcagccact
    10381 ggtaacagga ttagcagagc gaggtatgta ggcggtgcta cagagttctt gaagtggtgg
    10441 cctaactacg gctacactag aagaacagta tttggtatct gcgctctgct gaagccagtt
    10501 accttcggaa aaagagttgg tagctcttga tccggcaaac aaaccaccgc tggtagcggt
    10561 ggtttttttg tttgcaagca gcagattacg cgcagaaaaa aaggatctca agaagatcct
    10621 ttgatctttt ctacggggtc tgacgctcag tggaacgaaa actcacgtta agggattttg
    10681 gtcatgagat tatcaaaaag gatcttcacc tagatccttt taaattaaaa atgaagtttt
    10741 aaatcaatct aaagtatata tgagtaaact tggtctgaca gttaccaatg cttaatcagt
    10801 gaggcaccta tctcagcgat ctgtctattt cgttcatcca tagttgcctg actccccgtc
    10861 gtgtagataa ctacgatacg ggagggctta ccatctggcc ccagtgctgc aatgataccg
    10921 cgagacccac gctcaccggc tccagattta tcagcaataa accagccagc cggaagggcc
    10981 gagcgcagaa gtggtcctgc aactttatcc gcctccatcc agtctattaa ttgttgccgg
    11041 gaagctagag taagtagttc gccagttaat agtttgcgca acgttgttgc cattgctaca
    11101 ggcatcgtgg tgtcacgctc gtcgtttggt atggcttcat tcagctccgg ttcccaacga
    11161 tcaaggcgag ttacatgatc ccccatgttg tgcaaaaaag cggttagctc cttcggtcct
    11221 ccgatcgttg tcagaagtaa gttggccgca gtgttatcac tcatggttat ggcagcactg
    11281 cataattctc ttactgtcat gccatccgta agatgctttt ctgtgactgg tgagtactca
    11341 accaagtcat tctgagaata gtgtatgcgg cgaccgagtt gctcttgccc ggcgtcaata
    11401 cgggataata ccgcgccaca tagcagaact ttaaaagtgc tcatcattgg aaaacgttct
    11461 tcggggcgaa aactctcaag gatcttaccg ctgttgagat ccagttcgat gtaacccact
    11521 cgtgcaccca actgatcttc agcatctttt actttcacca gcgtttctgg gtgagcaaaa
    11581 acaggaaggc aaaatgccgc aaaaaaggga ataagggcga cacggaaatg ttgaatactc
    11641 atactcttcc tttttcaata ttattgaagc atttatcagg gttattgtct catgagcgga
    11701 tacatatttg aatgtattta gaaaaataaa caaatagggg ttccgcgcac atttccccga
    11761 aaagtgccac ctgacgtcta agaaaccatt attatcatga cattaaccta taaaaatagg
    11821 cgtatcacga ggccctttcg tctcgcgcgt ttcggtgatg acggtgaaaa cctctgacac
    11881 atgcagctcc cggagacggt cacagcttgt ctgtaagcgg atgccgggag cagacaagcc
    11941 cgtcagggcg cgtcagcggg tgttggcggg tgtcggggct ggcttaacta tgcggcatca
    12001 gagcagattg tactgagagt gcaccatatg cggtgtgaaa taccgcacag atgcgtaagg
    12061 agaaaatacc gcatcaggaa attgtaagcg ttaatatttt gttaaaattc gcgttaaatt
    12121 tttgttaaat cagctcattt tttaaccaat aggccgaaat cggcaaaatc ccttataaat
    12181 caaaagaata gaccgagata gggttgagtg ttgttccagt ttggaacaag agtccactat
    12241 taaagaacgt ggactccaac gtcaaagggc gaaaaaccgt ctatcagggc gatggcccac
    12301 tacgtgaacc atcaccctaa tcaagttttt tggggtcgag gtgccgtaaa gcactaaatc
    12361 ggaaccctaa agggagcccc cgatttagag cttgacgggg aaagccggcg aacgtggcga
    12421 gaaaggaagg gaagaaagcg aaaggagcgg gcgctagggc gctggcaagt gtagcggtca
    12481 cgctgcgcgt aaccaccaca cccgccgcgc ttaatgcgcc gctacagggc gcgtccattc
    12541 gccattcagg ctgcgcaact gttgggaagg gcgatcggtg cgggcctctt cgctattacg
    12601 ccagctggcg aaagggggat gtgctgcaag gcgattaagt t
//
