LOCUS       sequence-176929-         749 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Modified version of lentiSAM v2, a lenti sgRNA cloning backbone with
            MS2 loops at tetraloop/stemloop 2, dCas9-VP64, and puro resistance 
            marker. Contains BsmBI sites for insertion of spacer sequences..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 749)
  AUTHORS   Barger CJ, Chee L, Albahrani M, Munoz-Trujillo C, Boghean L, Branick
            C, Odunsi K, Drapkin R, Zou L, Karpf AR
  TITLE     Co-regulation and function of FOXM1/RHNO1 bidirectional genes in 
            cancer.
  JOURNAL   Elife. 2021 Apr 23;10. pii: 55070. doi: 10.7554/eLife.55070.
  PUBMED    33890574
REFERENCE   2  (bases 1 to 749)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Elife."; 
            date: "2021-04-23"; volume: "10. pii: 55070. doi"; pages: " 
            10.7554/eLife.55070"
COMMENT     Sequence Label: lentiSAM v2 (Puro)
FEATURES             Location/Qualifiers
     source          1..749
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             42..98
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
     CDS             99..695
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="TEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIERV
                     TELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLAA
                     QQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETSA
                     PRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     592..612
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
ORIGIN
        1 atttcgacct ggacatgctg attaactgta caggcagtgg agcaacaaac ttctctctgc
       61 tgaaacaagc cggagatgtc gaagagaatc ctggaccgac cgagtacaag cccacggtgc
      121 gcctcgccac ccgcgacgac gtccccaggg ccgtacgcac cctcgccgcc gcgttcgccg
      181 actaccccgc cacgcgccac accgtcgatc cggaccgcca catcgagcgg gtcaccgagc
      241 tgcaagaact cttcctcacg cgcgtcgggc tcgacatcgg caaggtgtgg gtcgcggacg
      301 acggcgccgc ggtggcggtc tggaccacgc cggagagcgt cgaagcgggg gcggtgttcg
      361 ccgagatcgg cccgcgcatg gccgagttga gcggttcccg gctggccgcg cagcaacaga
      421 tggaaggcct cctggcgccg caccggccca aggagcccgc gtggttcctg gccaccgtcg
      481 gagtctcgcc cgaccaccag ggcaagggtc tgggcagcgc cgtcgtgctc cccggagtgg
      541 aggcggccga gcgcgccggg gtgcccgcct tcctggagac ctccgcgccc cgcaacctcc
      601 ccttctacga gcggctcggc ttcaccgtca ccgccgacgt cgaggtgccc gaaggaccgc
      661 gcacctggtg catgacccgc aagcccggtg cctgaacgcg ttaagtcgaa ttcgatatca
      721 agcttatcgg taatcaacct ctggattac
//
