LOCUS       sequence-176940-       13502 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  retroviral mammalian expression vector for human p210BCR-ABL and 
            GFP.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 13502)
  TITLE     Roussel lab plasmids
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 13502)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: MSCV-(pBabe mcs)- human p210BCR-ABL-IRES-GFP
FEATURES             Location/Qualifiers
     source          1..13502
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     LTR             1..517
                     /label=5' LTR
                     /note="5' long terminal repeat from murine embryonic stem 
                     cell virus"
     misc_feature    581..922
                     /label=MESV Psi
                     /note="packaging signal of murine embryonic stem cell 
                     virus"
     CDS             989..1405
                     /label=gag (truncated)
                     /note="truncated Moloney murine leukemia virus (MMLV) gag 
                     gene lacking the start codon"
     primer_bind     1335..1357
                     /label=pLXSN 5'
                     /note="Murine stem cell virus, forward primer. Also called 
                     MSCV"
     primer_bind     1373..1389
                     /label=pBABE 5'
                     /note="Psi packaging signal, forward primer for pBABE 
                     vectors"
     misc_feature    8464..9013
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(8608..8625)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     8835..8854
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     CDS             join(9016..9018,9019..9021,9022..9735)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             join(9016..9018,9019..9021,9022..9732)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(9061..9082)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(9322..9341)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     9669..9690
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     LTR             9897..10411
                     /label=3' LTR
                     /note="3' long terminal repeat from murine embryonic stem 
                     cell virus"
     protein_bind    10573..10589
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(10597..10603,10604..10621,10622..10627))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    10642..10663
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(10780..10797)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(10951..11539)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(11031..11050)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(11710..12501,12502..12570))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     12333..12352
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(12571..12675)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     12743..12761
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     complement(12799..12821)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     12921..12940
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     13134..13156
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     13284..13303
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
ORIGIN
        1 aatgaaagac cccacctgta ggtttggcaa gctagcttaa gtaacgccat tttgcaaggc
       61 atggaaaata cataactgag aatagagaag ttcagatcaa ggttaggaac agagagacag
      121 cagaatatgg gccaaacagg atatctgtgg taagcagttc ctgccccggc tcagggccaa
      181 gaacagatgg tccccagatg cggtcccgcc ctcagcagtt tctagagaac catcagatgt
      241 ttccagggtg ccccaaggac ctgaaatgac cctgtgcctt atttgaacta accaatcagt
      301 tcgcttctcg cttctgttcg cgcgcttctg ctccccgagc tcaataaaag agcccacaac
      361 ccctcactcg gcgcgccagt cctccgatag actgcgtcgc ccgggtaccc gtattcccaa
      421 taaagcctct tgctgtttgc atccgaatcg tggactcgct gatccttggg agggtctcct
      481 cagattgatt gactgcccac ctcgggggtc tttcatttgg aggttccacc gagatttgga
      541 gacccctgcc cagggaccac cgaccccccc gccgggaggt aagctggcca gcggtcgttt
      601 cgtgtctgtc tctgtctttg tgcgtgtttg tgccggcatc taatgtttgc gcctgcgtct
      661 gtactagtta gctaactagc tctgtatctg gcggacccgt ggtggaactg acgagttctg
      721 aacacccggc cgcaaccctg ggagacgtcc cagggacttt gggggccgtt tttgtggccc
      781 gacctgagga agggagtcga tgtggaatcc gaccccgtca ggatatgtgg ttctggtagg
      841 agacgagaac ctaaaacagt tcccgcctcc gtctgaattt ttgctttcgg tttggaaccg
      901 aagccgcgcg tcttgtctgc tgcagcgctg cagcatcgtt ctgtgttgtc tctgtctgac
      961 tgtgtttctg tatttgtctg aaaattaggg ccagactgtt accactccct taagtttgac
     1021 cttaggtcac tggaaagatg tcgagcggat cgctcacaac cagtcggtag atgtcaagaa
     1081 gagacgttgg gttaccttct gctctgcaga atggccaacc tttaacgtcg gatggccgcg
     1141 agacggcacc tttaaccgag acctcatcac ccaggttaag atcaaggtct tttcacctgg
     1201 cccgcatgga cacccagacc aggtccccta catcgtgacc tgggaagcct tggcttttga
     1261 cccccctccc tgggtcaagc cctttgtaca ccctaagcct ccgcctcctc ttcctccatc
     1321 cgccccgtct ctcccccttg aacctcctcg ttcgaccccg cctcgatcct ccctttatcc
     1381 agccctcact ccttctctag gcgccggaat tgggatccca gtgtggtggt acgtaggaat
     1441 tccccggcgc ccggggccgg gcctggcgag gccgccgcgc cgccgctgag acgggccccg
     1501 cgcgcagccc ggcggcgcag gtaaggccgg ccgcgccatg gtggacccgg tgggcttcgc
     1561 ggaggcgtgg aaggcgcagt tcccggactc agagcccccg cgcatggagc tgcgctcagt
     1621 gggcgacatc gagcaggagc tggagcgctg caaggcctcc attcggcgcc tggagcagga
     1681 ggtgaaccag gagcgcttcc gcatgatcta cctgcagacg ttgctggcca aggaaaagaa
     1741 gagctatgac cggcagcgat ggggcttccg gcgcgcggcg caggcccccg acggcgcctc
     1801 cgagccccga gcgtccgcgt cgcgcccgca gccagcgccc gccgacggag ccgacccgcc
     1861 gcccgccgag gagcccgagg cccggcccga cggcgagggt tctccgggta aggccaggcc
     1921 cgggaccgcc cgcaggcccg gggcagccgc gtcgggggaa cgggacgacc ggggaccccc
     1981 cgccagcgtg gcggcgctca ggtccaactt cgagcggatc cgcaagggcc atggccagcc
     2041 cggggcggac gccgagaagc ccttctacgt gaacgtcgag tttcaccacg agcgcggcct
     2101 ggtgaaggtc aacgacaaag aggtgtcgga ccgcatcagc tccctgggca gccaggccat
     2161 gcagatggag cgcaaaaagt cccagcacgg cgcgggctcg agcgtggggg atgcatccag
     2221 gcccccttac cggggacgct cctcggagag cagctgcggc gtcgacggcg actacgagga
     2281 cgccgagttg aacccccgct tcctgaagga caacctgatc gacgccaatg gcggtagcag
     2341 gcccccttgg ccgcccctgg agtaccagcc ctaccagagc atctacgtcg ggggcatgat
     2401 ggaaggggag ggcaagggcc cgctcctgcg cagccagagc acctctgagc aggagaagcg
     2461 ccttacctgg ccccgcaggt cctactcccc ccggagtttt gaggattgcg gaggcggcta
     2521 taccccggac tgcagctcca atgagaacct cacctccagc gaggaggact tctcctctgg
     2581 ccagtccagc cgcgtgtccc caagccccac cacctaccgc atgttccggg acaaaagccg
     2641 ctctccctcg cagaactcgc aacagtcctt cgacagcagc agtcccccca cgccgcagtg
     2701 ccataagcgg caccggcact gcccggttgt cgtgtccgag gccaccatcg tgggcgtccg
     2761 caagaccggg cagatctggc ccaacgatgg cgagggcgcc ttccatggag acgcagatgg
     2821 ctcgttcgga acaccacctg gatacggctg cgctgcagac cgggcagagg agcagcgccg
     2881 gcaccaagat gggctgccct acattgatga ctcgccctcc tcatcgcccc acctcagcag
     2941 caagggcagg ggcagccggg atgcgctggt ctcgggagcc ctggagtcca ctaaagcgag
     3001 tgagctggac ttggaaaagg gcttggagat gagaaaatgg gtcctgtcgg gaatcctggc
     3061 tagcgaggag acttacctga gccacctgga ggcactgctg ctgcccatga agcctttgaa
     3121 agccgctgcc accacctctc agccggtgct gacgagtcag cagatcgaga ccatcttctt
     3181 caaagtgcct gagctctacg agatccacaa ggagttctat gatgggctct tcccccgcgt
     3241 gcagcagtgg agccaccagc agcgggtggg cgacctcttc cagaagctgg ccagccagct
     3301 gggtgtgtac cgggccttcg tggacaacta cggagttgcc atggaaatgg ctgagaagtg
     3361 ctgtcaggcc aatgctcagt ttgcagaaat ctccgagaac ctgagagcca gaagcaacaa
     3421 agatgccaag gatccaacga ccaagaactc tctggaaact ctgctctaca agcctgtgga
     3481 ccgtgtgacg aggagcacgc tggtcctcca tgacttgctg aagcacactc ctgccagcca
     3541 ccctgaccac cccttgctgc aggacgccct ccgcatctca cagaacttcc tgtccagcat
     3601 caatgaggag atcacacccc gacggcagtc catgacggtg aagaagggag agcaccggca
     3661 gctgctgaag gacagcttca tggtggagct ggtggagggg gcccgcaagc tgcgccacgt
     3721 cttcctgttc accgacctgc ttctctgcac caagctcaag aagcagagcg gaggcaaaac
     3781 gcagcagtat gactgcaaat ggtacattcc gctcacggat ctcagcttcc agatggtgga
     3841 tgaactggag gcagtgccca acatccccct ggtgcccgat gaggagctgg acgctttgaa
     3901 gatcaagatc tcccagatca agagtgacat ccagagagag aagagggcga acaagggcag
     3961 caaggctacg gagaggctga agaagaagct gtcggagcag gagtcactgc tgctgcttat
     4021 gtctcccagc atggccttca gggtgcacag ccgcaacggc aagagttaca cgttcctgat
     4081 ctcctctgac tatgagcgtg cagagtggag ggagaacatc cgggagcagc agaagaagtg
     4141 tttcagaagc ttctccctga catccgtgga gctgcagatg ctgaccaact cgtgtgtgaa
     4201 actccagact gtccacagca ttccgctgac catcaataag gaagatgatg agtctccggg
     4261 gctctatggg tttctgaatg tcatcgtcca ctcagccact ggatttaagc agagttcaaa
     4321 agcccttcag cggccagtag catctgactt tgagcctcag ggtctgagtg aagccgctcg
     4381 ttggaactcc aaggaaaacc ttctcgctgg acccagtgaa aatgacccca accttttcgt
     4441 tgcactgtat gattttgtgg ccagtggaga taacactcta agcataacta aaggtgaaaa
     4501 gctccgggtc ttaggctata atcacaatgg ggaatggtgt gaagcccaaa ccaaaaatgg
     4561 ccaaggctgg gtcccaagca actacatcac gccagtcaac agtctggaga aacactcctg
     4621 gtaccatggg cctgtgtccc gcaatgccgc tgagtatctg ctgagcagcg ggatcaatgg
     4681 cagcttcttg gtgcgtgaga gtgagagcag tcctggccag aggtccatct cgctgagata
     4741 cgaagggagg gtgtaccatt acaggatcaa cactgcttct gatggcaagc tctacgtctc
     4801 ctccgagagc cgcttcaaca ccctggccga gttggttcat catcattcaa cggtggccga
     4861 cgggctcatc accacgctcc attatccagc cccaaagcgc aacaagccca ctgtctatgg
     4921 tgtgtccccc aactacgaca agtgggagat ggaacgcacg gacatcacca tgaagcacaa
     4981 gctgggcggg ggccagtacg gggaggtgta cgagggcgtg tggaagaaat acagcctgac
     5041 ggtggccgtg aagaccttga aggaggacac catggaggtg gaagagttct tgaaagaagc
     5101 tgcagtcatg aaagagatca aacaccctaa cctggtgcag ctccttgggg tctgcacccg
     5161 ggagcccccg ttctatatca tcactgagtt catgacctac gggaacctcc tggactacct
     5221 gagggagtgc aaccggcagg aggtgaacgc cgtggtgctg ctgtacatgg ccactcagat
     5281 ctcgtcagcc atggagtacc tggagaagaa aaacttcatc cacagagatc ttgctgcccg
     5341 aaactgcctg gtaggggaga accacttggt gaaggtagct gattttggcc tgagcaggtt
     5401 gatgacaggg gacacctaca cagcccatgc tggagccaag ttccccatca aatggactgc
     5461 acccgagagc ctggcctaca acaagttctc catcaagtcc gacgtctggg catttggagt
     5521 attgctttgg gaaattgcta cctatggcat gtccccttac ccgggaattg acctgtccca
     5581 ggtgtatgag ctgctagaga aggactaccg catggagcgc ccagaaggct gcccagagaa
     5641 ggtctatgaa ctcatgcgag catgttggca gtggaatccc tctgaccggc cctcctttgc
     5701 tgaaatccac caagcctttg aaacaatgtt ccaggaatcc agtatctcag acgaagtgga
     5761 aaaggagctg gggaaacaag gcgtccgtgg ggctgtgagt accttgctgc aggccccaga
     5821 gctgcccacc aagacgagga cctccaggag agctgcagag cacagagaca ccactgacgt
     5881 gcctgagatg cctcactcca agggccaggg agagagcgat cctctggacc atgagcctgc
     5941 cgtgtctcca ttgctccctc gaaaagagcg aggtcccccg gagggcggcc tgaatgaaga
     6001 tgagcgcctt ctccccaaag acaaaaagac caacttgttc agcgccttga tcaagaagaa
     6061 gaagaagaca gccccaaccc ctcccaaacg cagcagctcc ttccgggaga tggacggcca
     6121 gccggagcgc agaggggccg gcgaggaaga gggccgagac atcagcaacg gggcactggc
     6181 tttcaccccc ttggacacag ctgacccagc caagtcccca aagcccagca atggggctgg
     6241 ggtccccaat ggagccctcc gggagtccgg gggctcaggc ttccggtctc cccacctgtg
     6301 gaagaagtcc agcacgctga ccagcagccg cctagccacc ggcgaggagg agggcggtgg
     6361 cagctccagc aagcgcttcc tgcgctcttg ctccgcctcc tgcgttcccc atggggccaa
     6421 ggacacggag tggaggtcag tcacgctgcc tcgggacttg cagtccacgg gaagacagtt
     6481 tgactcgtcc acatttggag ggcacaaaag tgagaagccg gctctgcctc ggaagagggc
     6541 aggggagaac aggtctgacc aggtgacccg aggcacagta acgcctcccc ccaggctggt
     6601 gaaaaagaat gaggaagctg ctgatgaggt cttcaaagac atcatggagt ccagcccggg
     6661 ctccagcccg cccaacctga ctccaaaacc cctccggcgg caggtcaccg tggcccctgc
     6721 ctcgggcctc ccccacaagg aagaagctgg aaagggcagt gccttaggga cccctgctgc
     6781 agctgagcca gtgaccccca ccagcaaagc aggctcaggt gcaccagggg gcaccagcaa
     6841 gggccccgcc gaggagtcca gagtgaggag gcacaagcac tcctctgagt cgccagggag
     6901 ggacaagggg aaattgtcca ggctcaaacc tgccccgccg cccccaccag cagcctctgc
     6961 agggaaggct ggaggaaagc cctcgcagag cccgagccag gaggcggccg gggaggcagt
     7021 cctgggcgca aagacaaaag ccacgagtct ggttgatgct gtgaacagtg acgctgccaa
     7081 gcccagccag ccgggagagg gcctcaaaaa gcccgtgctc ccggccactc caaagccaca
     7141 gtccgccaag ccgtcgggga cccccatcag cccagccccc gttccctcca cgttgccatc
     7201 agcatcctcg gccctggcag gggaccagcc gtcttccacc gccttcatcc ctctcatatc
     7261 aacccgagtg tctcttcgga aaacccgcca gcctccagag cggatcgcca gcggcgccat
     7321 caccaagggc gtggtcctgg acagcaccga ggcgctgtgc ctcgccatct ctaggaactc
     7381 cgagcagatg gccagccaca gcgcagtgct ggaggccggc aaaaacctct actcgttctg
     7441 cgtgagctat gtggattcca tccagcaaat gaggaacaag tttgccttcc gagaggccat
     7501 caacaaactg gagaataatc tccgggagct tcagatctgc ccggcgacag caggcagtgg
     7561 tccagcggcc actcaggact tcagcaagct cctcagttcg gtgaaggaaa tcagtgacat
     7621 agtgcagagg tagcagcagt caggggtcag gtgtcaggcc cgtcggagct gcctgcagca
     7681 catgcgggct cgcccatacc cgtgacagtg gctgacaagg gactagtgag tcagcacctt
     7741 ggcccaggag ctctgcgcca ggcagagctg agggccctgt ggagtccagc tctactacct
     7801 acgtttgcac cgcctgccct cccgcacctt cctcctcccc gctccgtctc tgtcctcgaa
     7861 ttttatctgt ggagttcctg ctccgtggac tgcagtcggc atgccaggac ccgccagccc
     7921 cgctcccacc tagtgcccca gactgagctc tccaggccag gtgggaacgg ctgatgtgga
     7981 ctgtcttttt catttttttc tctctggagc ccctcctccc ccggctgggc ctccttcttc
     8041 cacttctcca agaatggaag cctgaactga ggccttgtgt gtcaggccct ctgcctgcac
     8101 tccctggcct tgcccgtcgt gtgctgaaga catgtttcaa gaaccgcatt tcgggaaggg
     8161 catgcacggg catgcacacg gctggtcact ctgccctctg ctgctgcccg gggtggggtg
     8221 cactcgccat ttcctcacgt gcaggacagc tcttgatttg ggtggaaaac agggtgctaa
     8281 agccaaccag cctttgggtc ctgggcaggt gggagctgaa aaggatcgag gcatggggca
     8341 tgtcctttcc atctgtccac atccccagag cccagctctt gctctcttgt gacgtgcact
     8401 gtgaatcctg gcaagaaagc tggaattcag atctcgagcg ggatcaattc cgcccccccc
     8461 ctaacgttac tggccgaagc cgcttggaat aaggccggtg tgcgtttgtc tatatgttat
     8521 tttccaccat attgccgtct tttggcaatg tgagggcccg gaaacctggc cctgtcttct
     8581 tgacgagcat tcctaggggt ctttcccctc tcgccaaagg aatgcaaggt ctgttgaatg
     8641 tcgtgaagga agcagttcct ctggaagctt cttgaagaca aacaacgtct gtagcgaccc
     8701 tttgcaggca gcggaacccc ccacctggcg acaggtgcct ctgcggccaa aagccacgtg
     8761 tataagatac acctgcaaag gcggcacaac cccagtgcca cgttgtgagt tggatagttg
     8821 tggaaagagt caaatggctc tcctcaagcg tattcaacaa ggggctgaag gatgcccaga
     8881 aggtacccca ttgtatggga tctgatctgg ggcctcggtg cacatgcttt acatgtgttt
     8941 agtcgaggtt aaaaaaacgt ctaggccccc cgaaccacgg ggacgtggtt ttcctttgaa
     9001 aaacacgata ataccatggt gagcaagggc gaggagctgt tcaccggggt ggtgcccatc
     9061 ctggtcgagc tggacggcga cgtaaacggc cacaagttca gcgtgtccgg cgagggcgag
     9121 ggcgatgcca cctacggcaa gctgaccctg aagttcatct gcaccaccgg caagctgccc
     9181 gtgccctggc ccaccctcgt gaccaccctg acctacggcg tgcagtgctt cagccgctac
     9241 cccgaccaca tgaagcagca cgacttcttc aagtccgcca tgcccgaagg ctacgtccag
     9301 gagcgcacca tcttcttcaa ggacgacggc aactacaaga cccgcgccga ggtgaagttc
     9361 gagggcgaca ccctggtgaa ccgcatcgag ctgaagggca tcgacttcaa ggaggacggc
     9421 aacatcctgg ggcacaagct ggagtacaac tacaacagcc acaacgtcta tatcatggcc
     9481 gacaagcaga agaacggcat caaggtgaac ttcaagatcc gccacaacat cgaggacggc
     9541 agcgtgcagc tcgccgacca ctaccagcag aacaccccca tcggcgacgg ccccgtgctg
     9601 ctgcccgaca accactacct gagcacccag tccgccctga gcaaagaccc caacgagaag
     9661 cgcgatcaca tggtcctgct ggagttcgtg accgccgccg ggatcactct cggcatggac
     9721 gagctgtaca agtaaagcgg ccgcgactct agagtcgacc tgcaggcatg caagcttcag
     9781 gtagccggct aacgttaaca accggtacct ctagaactat agctagcatg cgcaaattta
     9841 aagcgctgat atcgataaaa taaaagattt tatttagtct ccagaaaaag gggggaatga
     9901 aagaccccac ctgtaggttt ggcaagctag cttaagtaac gccattttgc aaggcatgga
     9961 aaatacataa ctgagaatag agaagttcag atcaaggtta ggaacagaga gacagcagaa
    10021 tatgggccaa acaggatatc tgtggtaagc agttcctgcc ccggctcagg gccaagaaca
    10081 gatggtcccc agatgcggtc ccgccctcag cagtttctag agaaccatca gatgtttcca
    10141 gggtgcccca aggacctgaa atgaccctgt gccttatttg aactaaccaa tcagttcgct
    10201 tctcgcttct gttcgcgcgc ttctgctccc cgagctcaat aaaagagccc acaacccctc
    10261 actcggcgcg ccagtcctcc gatagactgc gtcgcccggg tacccgtgta tccaataaac
    10321 cctcttgcag ttgcatccga cttgtggtct cgctgttcct tgggagggtc tcctctgagt
    10381 gattgactac ccgtcagcgg gggtctttca tgggtaacag tttcttgaag ttggagaaca
    10441 acattctgag ggtaggagtc gaatattaag taatcctgac tcaattagcc actgttttga
    10501 atccacatac tccaatactc ctgaaatagt tcattatgga cagcgcagaa agagctgggg
    10561 agaattgtga aattgttatc cgctcacaat tccacacaac atacgagccg gaagcataaa
    10621 gtgtaaagcc tggggtgcct aatgagtgag ctaactcaca ttaattgcgt tgcgctcact
    10681 gcccgctttc cagtcgggaa acctgtcgtg ccagctgcat taatgaatcg gccaacgcgc
    10741 ggggagaggc ggtttgcgta ttgggcgctc ttccgcttcc tcgctcactg actcgctgcg
    10801 ctcggtcgtt cggctgcggc gagcggtatc agctcactca aaggcggtaa tacggttatc
    10861 cacagaatca ggggataacg caggaaagaa catgtgagca aaaggccagc aaaaggccag
    10921 gaaccgtaaa aaggccgcgt tgctggcgtt tttccatagg ctccgccccc ctgacgagca
    10981 tcacaaaaat cgacgctcaa gtcagaggtg gcgaaacccg acaggactat aaagatacca
    11041 ggcgtttccc cctggaagct ccctcgtgcg ctctcctgtt ccgaccctgc cgcttaccgg
    11101 atacctgtcc gcctttctcc cttcgggaag cgtggcgctt tctcatagct cacgctgtag
    11161 gtatctcagt tcggtgtagg tcgttcgctc caagctgggc tgtgtgcacg aaccccccgt
    11221 tcagcccgac cgctgcgcct tatccggtaa ctatcgtctt gagtccaacc cggtaagaca
    11281 cgacttatcg ccactggcag cagccactgg taacaggatt agcagagcga ggtatgtagg
    11341 cggtgctaca gagttcttga agtggtggcc taactacggc tacactagaa gaacagtatt
    11401 tggtatctgc gctctgctga agccagttac cttcggaaaa agagttggta gctcttgatc
    11461 cggcaaacaa accaccgctg gtagcggtgg tttttttgtt tgcaagcagc agattacgcg
    11521 cagaaaaaaa ggatctcaag aagatccttt gatcttttct acggggtctg acgctcagtg
    11581 gaacgaaaac tcacgttaag ggattttggt catgagatta tcaaaaagga tcttcaccta
    11641 gatcctttta aattaaaaat gaagttttaa atcaatctaa agtatatatg agtaaacttg
    11701 gtctgacagt taccaatgct taatcagtga ggcacctatc tcagcgatct gtctatttcg
    11761 ttcatccata gttgcctgac tccccgtcgt gtagataact acgatacggg agggcttacc
    11821 atctggcccc agtgctgcaa tgataccgcg agacccacgc tcaccggctc cagatttatc
    11881 agcaataaac cagccagccg gaagggccga gcgcagaagt ggtcctgcaa ctttatccgc
    11941 ctccatccag tctattaatt gttgccggga agctagagta agtagttcgc cagttaatag
    12001 tttgcgcaac gttgttgcca ttgctacagg catcgtggtg tcacgctcgt cgtttggtat
    12061 ggcttcattc agctccggtt cccaacgatc aaggcgagtt acatgatccc ccatgttgtg
    12121 caaaaaagcg gttagctcct tcggtcctcc gatcgttgtc agaagtaagt tggccgcagt
    12181 gttatcactc atggttatgg cagcactgca taattctctt actgtcatgc catccgtaag
    12241 atgcttttct gtgactggtg agtactcaac caagtcattc tgagaatagt gtatgcggcg
    12301 accgagttgc tcttgcccgg cgtcaatacg ggataatacc gcgccacata gcagaacttt
    12361 aaaagtgctc atcattggaa aacgttcttc ggggcgaaaa ctctcaagga tcttaccgct
    12421 gttgagatcc agttcgatgt aacccactcg tgcacccaac tgatcttcag catcttttac
    12481 tttcaccagc gtttctgggt gagcaaaaac aggaaggcaa aatgccgcaa aaaagggaat
    12541 aagggcgaca cggaaatgtt gaatactcat actcttcctt tttcaatatt attgaagcat
    12601 ttatcagggt tattgtctca tgagcggata catatttgaa tgtatttaga aaaataaaca
    12661 aataggggtt ccgcgcacat ttccccgaaa agtgccacct gacgtctaag aaaccattat
    12721 tatcatgaca ttaacctata aaaataggcg tatcacgagg ccctttcgtc tcgcgcgttt
    12781 cggtgatgac ggtgaaaacc tctgacacat gcagctcccg gagacggtca cagcttgtct
    12841 gtaagcggat gccgggagca gacaagcccg tcagggcgcg tcagcgggtg ttggcgggtg
    12901 tcggggctgg cttaactatg cggcatcaga gcagattgta ctgagagtgc accatatgcg
    12961 gtgtgaaata ccgcacagat gcgtaaggag aaaataccgc atcaggcgcc attcgccatt
    13021 caggctgcgc aactgttggg aagggcgatc ggtgcgggcc tcttcgctat tacgccagct
    13081 ggcgaaaggg ggatgtgctg caaggcgatt aagttgggta acgccagggt tttcccagtc
    13141 acgacgttgt aaaacgacgg cgcaaggaat ggtgcatgca aggagatggc gcccaacagt
    13201 cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag
    13261 tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct
    13321 gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggcgattagt ccaatttgtt
    13381 aaagacagga tatcagtggt ccaggctcta gttttgactc aacaatatca ccagctgaag
    13441 cctatagagt acgagccata gataaaataa aagattttat ttagtctcca gaaaaagggg
    13501 gg
//
