LOCUS       sequence-176957-         860 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentiviral vector with dox inducible expression of shRNA targeting 
            human FOXM1..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 860)
  TITLE     FOXM1 toolbox
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 860)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: Tet-pLKO-FOXM1-shRNA-84
FEATURES             Location/Qualifiers
     source          1..860
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        91..185
                     /label=H1-2O2 promoter
                     /note="doxycycline-inducible variant of the human H1 RNA 
                     promoter (Henriksen et al., 2007)"
     primer_bind     102..121
                     /label=H1
                     /note="Human H1 promoter, forward primer"
     protein_bind    139..157
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    167..185
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     misc_feature    286..403
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
ORIGIN
        1 gaaggtggag agagagacag agacagatcc attcgattag tgaacggatc tcgacggtat
       61 cgatcacgag actagcctcg agcggccgca atatttgcat gtcgctatgt gttctgggaa
      121 atcaccataa acgtgaaatc cctatcagtg atagagactt ataagttccc tatcagtgat
      181 agagacaccg gttgcagggt ggtccgtgta aactcgagtt tacacggacc accctgcaat
      241 ttttgaattc tcgacctcga gacaaatggc agtattcatc cacaatttta aaagaaaagg
      301 ggggattggg gggtacagtg caggggaaag aatagtagac ataatagcaa cagacataca
      361 aactaaagaa ttacaaaaac aaattacaaa aattcaaaat tttcgggttt attacaggga
      421 cagcagagat ccactttggc cgcggctcga gggggttggg gttgcgcctt ttccaaggca
      481 gccctgggtt tgcgcaggga cgcggctgct ctgggcgtgg ttccgggaaa cgcagcggcg
      541 ccgaccctgg gtctcgcaca ttcttcacgt ccgttcgcag cgtcacccgg atcttcgccg
      601 ctacccttgt gggccccccg gcgacgcttc ctgctccgcc cctaagtcgg gaaggttcct
      661 tgcggttcgc ggcgtgccgg acgtgacaac ggaagccgca cgtctcacta gtaccctcgc
      721 agacggacag cgccagggag caatggcagc gcgcgaccgc gatgggctgt ggccaatagc
      781 ggctgctcag cagggcgcgc cgagagcagc ggccgggtaa gggcgtgctg yaggcagggt
      841 gtgggcgtag tgtggcctgg
//
