LOCUS       sequence-176959-         578 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentiviral vector with constitutive expression of shRNA targeting 
            human FOXM1..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 578)
  TITLE     FOXM1 toolbox
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 578)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: pLKO-FOXM1-shRNA-84
FEATURES             Location/Qualifiers
     source          1..578
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        127..375
                     /label=U6 promoter
                     /note="RNA polymerase III promoter for human U6 snRNA 
                     (Domitrovich & Kunkel, 2003)"
     primer_bind     127..147
                     /label=hU6-F
                     /note="Human U6 promoter, forward primer"
     primer_bind     298..317
                     /label=LKO.1 5'
                     /note="Human U6 promoter, forward primer"
ORIGIN
        1 accgacaggc ccgaaggaat agaagaagaa ggtggagaga gagacagaga cagatccatt
       61 cgattagtga acggatctcg acggtatcga tcacgagact agcctcgagc ggccgccccc
      121 ttcaccgagg gcctatttcc catgattcct tcatatttgc atatacgata caaggctgtt
      181 agagagataa ttggaattaa tttgactgta aacacaaaga tattagtaca aaatacgtga
      241 cgtagaaagt aataatttct tgggtagttt gcagttttaa aattatgttt taaaatggac
      301 tatcatatgc ttaccgtaac ttgaaagtat ttcgatttct tggctttata tatcttgtgg
      361 aaaggacgaa acaccggttg cagggtggtc cgtgtaaact cgagtttaca cggaccaccc
      421 tgcatttttg aattctcgac ctcgagacaa atggcagtat tcatccacaa ttttaaaaga
      481 aaagggggga ttggggggta caktgcaggg gaaagaatag tagayataat agcawcagac
      541 atacaaaact aaagaattac aaayacaawt tacaaaaa
//
