LOCUS       sequence-176961-         714 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentiviral vector with dox inducible expression of shRNA targeting 
            human FOXM1..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 714)
  TITLE     FOXM1 toolbox
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 714)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: Tet-pLKO-FOXM1-shRNA-#2
FEATURES             Location/Qualifiers
     source          1..714
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        46..140
                     /label=H1-2O2 promoter
                     /note="doxycycline-inducible variant of the human H1 RNA 
                     promoter (Henriksen et al., 2007)"
     primer_bind     57..76
                     /label=H1
                     /note="Human H1 promoter, forward primer"
     protein_bind    94..112
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    122..140
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     misc_feature    240..357
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
ORIGIN
        1 ggatctcgac ggtatcgatc acgagactag cctcgagcgg ccgcaatatt tgcatgtcgc
       61 tatgtgttct gggaaatcac cataaacgtg aaatccctat cagtgataga gacttataag
      121 ttccctatca gtgatagaga caccggcctt tccctgcacg acatgttcaa gagacatgtc
      181 gtgcagggaa aggtttttga attctcgacc tcgagacaaa tggcagtatt catccacaat
      241 tttaaaagaa aaggggggat tggggggtac agtgcagggg aaagaatagt agacataata
      301 gcaacagaca tacaaactaa agaattacaa aaacaaatta caaaaattca aaattttcgg
      361 gtttattaca gggacagcag agatccactt tggccgcggc tcgagggggt tggggttgcg
      421 ccttttccaa ggcagccctg ggtttgcgca gggacgcggc tgctctgggc gtggttccgg
      481 gaaacgcagc ggcgccgacc ctgggtctcg cacattcttc acgtccgttc gcagcgtcac
      541 ccggatcttc gccgctaccc ttgtgggccc cccggcgacg cttcctgctc cgcccctaag
      601 tcgggaaggt tccttgcggt tcgcggcgtg ccggacgtga caaacggaag ccgcacgtct
      661 cactagtacc ctcgcagacg gacagcgcca gggagcaatg gcagcgcgcg acgc
//
