LOCUS       sequence-176964-        4064 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Constitutive transcription of crRNA of crtYf, Chloramphenicol 
            resistance.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4064)
  AUTHORS   Jiang Y, Qian F, Yang J, Liu Y, Dong F, Xu C, Sun B, Chen B, Xu X, 
            Li Y, Wang R, Yang S
  TITLE     CRISPR-Cpf1 assisted genome editing of Corynebacterium glutamicum.
  JOURNAL   Nat Commun. 2017 May 4;8:15179. doi: 10.1038/ncomms15179.
  PUBMED    28469274
REFERENCE   2  (bases 1 to 4064)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2017-05-4"; volume: "8:15179. doi"; pages: " 
            10.1038/ncomms15179"
COMMENT     Sequence Label: pJYS2cm_crtYf
FEATURES             Location/Qualifiers
     source          1..4064
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      77..665
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     566..585
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     819..836
                     /label=L4440
                     /note="L4440 vector, forward primer"
     terminator      complement(930..957)
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     terminator      complement(1049..1134)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     CDS             complement(3245..3904)
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPELRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     3819..3838
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
ORIGIN
        1 tcatgaccaa aatcccttaa cgtgagtttt cgttccactg agcgtcagac cccgtagaaa
       61 agatcaaagg atcttcttga gatccttttt ttctgcgcgt aatctgctgc ttgcaaacaa
      121 aaaaaccacc gctaccagcg gtggtttgtt tgccggatca agagctacca actctttttc
      181 cgaaggtaac tggcttcagc agagcgcaga taccaaatac tgtccttcta gtgtagccgt
      241 agttaggcca ccacttcaag aactctgtag caccgcctac atacctcgct ctgctaatcc
      301 tgttaccagt ggctgctgcc agtggcgata agtcgtgtct taccgggttg gactcaagac
      361 gatagttacc ggataaggcg cagcggtcgg gctgaacggg gggttcgtgc acacagccca
      421 gcttggagcg aacgacctac accgaactga gatacctaca gcgtgagcta tgagaaagcg
      481 ccacgcttcc cgaagggaga aaggcggaca ggtatccggt aagcggcagg gtcggaacag
      541 gagagcgcac gagggagctt ccagggggaa acgcctggta tctttatagt cctgtcgggt
      601 ttcgccacct ctgacttgag cgtcgatttt tgtgatgctc gtcagggggg cggagcctat
      661 ggaaaaacgc cagcaacgcg gcctttttac ggttcctggc cttttgctgg ccttttgctc
      721 acatgttctt tcctgcgtta tcccctgatt ctgtggataa ccgtattacc gcctttgagt
      781 gagctgatac cgctcgccgc agccgaacga ccgagcgcag cgagtcagtg agcgaggaag
      841 cggaagagcg cctgatgcgg tattttctcc ttacgcatct gtgcggtatt tcacaccgca
      901 tatgaagctt aagagtttgt agaaacgcaa aaaggccatc cgtcaggatg gccttctgct
      961 taatttgatg cctggcagtt tatggcgggc gtcctgcccg ccaccctccg ggccgttgct
     1021 tcgcaacgtt caaatccgct cccggcggat ttgtcctact caggagagcg ttcaccgaca
     1081 aacaacagat aaaacgaaag gcccagtctt tcgactgagc ctttcgtttt atttaaattt
     1141 cctgccctat ggttgcctga tctacaacag tagaaattcg gatccattat acctaggact
     1201 gagctagctg tcaatctaga ctcgaggaga tttttgaggg ggagggagtc gaggaagagc
     1261 cagagcagaa ggcgggggaa ccgttctctg ccgacagcgt gagccccctt aaaaatcagg
     1321 ccggggagga accggggagg gatcagagct aggagcgaga caccctaaag ggggggaacc
     1381 gttttctgct gacggtgttt cgtttattag ttttcagccc gtggatagcg gagggtgagg
     1441 gcaagtgaga gccagagcaa ggacgggacc cctaaagggg ggaaccgttt tctgctgacg
     1501 gtgtttcgtt tattagtttt cagcccgtgg acggccgcgt ttagcttcca ttccaagtgc
     1561 ctttctgact tgttggatgc gcctttcact gacacctagt tcgcctgcaa gctcacgagt
     1621 cgagggatca gcaaccgatt gagaacgggc atccaggatc gcagttttga cgcgaagttc
     1681 gagcaactcg cctgtcattt ctcggcgttt gtttgcttcc gctaatcgct gtcgcgtctc
     1741 ctgcgcatac ttactttctg ggtcagccca tctgcgtgca ttcgatgtag ctgcgccccg
     1801 tcgccccatc gtcgctagag ctttccgccc tcggctgctc tgcgtttcca cccgacgagc
     1861 agggacgact ggctggcctt tagccacgta gccgcgcaca cgacgcgcca tcgtcaggcg
     1921 atcacgcatg gcgggaagat ccggctcccg gccgtctgca ccgaccgcct gggcaacgtt
     1981 gtacgccact tcatacgcgt cgatgatctt ggcatctttt aggcgctcac cagcagcttt
     2041 gagctggtat cccacggtca acgcgtggcg aaacgcggtc tcgtcgcgcg ctgcgcgctc
     2101 tggatttgtc cagagcactc gcacgccgtc gatcaggtcg ccggacgcgt ccagggcgct
     2161 cggcaggctc gcgtccaaaa tcgctagcgc cttggcttct gcggtggcgc gttgtgccgc
     2221 ttcaatgcgg gcgcgtccgc tggaaaagtc ctgctcaatg tactttttcg gcttctgtga
     2281 tccggtcatc gttcgagcaa tctccattag gtcggccagc cgatccacac gatcatgctg
     2341 gcagtgccat ttataggctg tcggatcgtc tgagacgtgc agcggccacc ggctcagcct
     2401 atgcgaaaaa gcctggtcag cgccgaaaac acgagtcatt tcttccgtcg ttgcagccag
     2461 caggcgcata tttgggctgg ttttacctgc tgcggcatac accgggtcaa tgagccagat
     2521 gagctggcat ttcccgctca gcggattcac gccgatccaa gccggcgctt tttctaggcg
     2581 tgcccatttc tctaaaatcg cgtagacctg cgggtttacg tgctcaatct tcccgccggc
     2641 ctggtggctg ggcacatcga tgtcaagcac gatcaccgcg gcatgttgcg cgtgcgtcag
     2701 cgcaacgtac tggcaccgcg tcagcgcttt tgagccagcc cggtagagct ttggttgggt
     2761 ttcgccggta tccgggtttt taatccaggc gctcgcgaaa tctcttgtct tgctgccctg
     2821 gaagctttcg cgtcccaggt gagcgagcag ttcgcggcga tcttctgccg tccagccgcg
     2881 tgagcgcagc gctagttcgg ggtgggtgtc gaacagatcg gcggaaaatt tccacgcgct
     2941 agctgtgact gtgtcctgcg gatcggctag agtcatgtct tgagtgcttt ctcccagctg
     3001 atgactgggg gttagccgac gccctgtgag ttcccgctca cggggcgttc aactttttca
     3061 ggtatttgtg cagcttatcg tgttttcttc gtaaatgaac gcttaactac cttgttaaac
     3121 gtggcaaata ggcaggattg atggctttca tagaaggcgg cggtggaatc gaaatctcgt
     3181 gatggcaggt tgggcgtcgc ttggtcggtc atttcgaagg gcaccaataa ctgccttaaa
     3241 aaaattacgc cccgccctgc cactcatcgc agtactgttg taattcatta agcattctgc
     3301 cgacatggaa gccatcacaa acggcatgat gaacctgaat cgccagcggc atcagcacct
     3361 tgtcgccttg cgtataatat ttgcccatgg tgaaaacggg ggcgaagaag ttgtccatat
     3421 tggccacgtt taaatcaaaa ctggtgaaac tcacccaggg attggctgag acgaaaaaca
     3481 tattctcaat aaacccttta gggaaatagg ccaggttttc accgtaacac gccacatctt
     3541 gcgaatatat gtgtagaaac tgccggaaat cgtcgtggta ttcactccag agcgatgaaa
     3601 acgtttcagt ttgctcatgg aaaacggtgt aacaagggtg aacactatcc catatcacca
     3661 gctcaccgtc tttcattgcc atacgtaact ccggatgagc attcatcagg cgggcaagaa
     3721 tgtgaataaa ggccggataa aacttgtgct tatttttctt tacggtcttt aaaaaggccg
     3781 taatatccag ctgaacggtc tggttatagg tacattgagc aactgactga aatgcctcaa
     3841 aatgttcttt acgatgccat tgggatatat caacggtggt atatccagtg atttttttct
     3901 ccattttagc ttccttagct cctgaaaatc tcgtcgaagc tcggcggatt tgtcctactc
     3961 aagctgatcc gacaaaatcc acacattatc acaggtgtcc ggatcggtca aatacgctgc
     4021 cagctcatag accgtatcca aagcatccgg ggctgatccc cggc
//
