LOCUS       sequence-176965-        4380 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Constitutive transcription of crRNA of crtYf, Spectinomycin 
            resistance.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4380)
  AUTHORS   Jiang Y, Qian F, Yang J, Liu Y, Dong F, Xu C, Sun B, Chen B, Xu X, 
            Li Y, Wang R, Yang S
  TITLE     CRISPR-Cpf1 assisted genome editing of Corynebacterium glutamicum.
  JOURNAL   Nat Commun. 2017 May 4;8:15179. doi: 10.1038/ncomms15179.
  PUBMED    28469274
REFERENCE   2  (bases 1 to 4380)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2017-05-4"; volume: "8:15179. doi"; pages: " 
            10.1038/ncomms15179"
COMMENT     Sequence Label: pJYS2_crtYf
FEATURES             Location/Qualifiers
     source          1..4380
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     terminator      3247..3332
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     terminator      3424..3451
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     primer_bind     complement(3545..3562)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(3716..4304)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(3796..3815)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 actaataacg taacgtgact ggcaagagat atttttaaaa caatgaatag gtttacactt
       61 actttagttt tatggaaatg aaagatcata tcatatataa tctagaataa aattaactaa
      121 aataattatt atctagataa aaaatttaga agccaatgaa atctataaat aaactaaatt
      181 aagtttattt aattaacaac tatggatata aaataggtac taatcaaaat agtgaggagg
      241 atatatttga atacatacga acaaattaat aaagtgaaaa aaatacttcg gaaacattta
      301 aaaaataacc ttattggtac ttacatgttt ggatcaggag ttgagagtgg actaaaacca
      361 aatagtgatc ttgacttttt agtcgtcgta tctgaaccat tgacagatca aagtaaagaa
      421 atacttatac aaaaaattag acctatttca aaaaaaatag gagataaaag caacttacga
      481 tatattgaat taacaattat tattcagcaa gaaatggtac cgtggaatca tcctcccaaa
      541 caagaattta tttatggaga atggttacaa gagctttatg aacaaggata cattcctcag
      601 aaggaattaa attcagattt aaccataatg ctttaccaag caaaacgaaa aaataaaaga
      661 atatacggaa attatgactt agaggaatta ctacctgata ttccattttc tgatgtgaga
      721 agagccatta tggattcgtc agaggaatta atagataatt atcaggatga tgaaaccaac
      781 tctatattaa ctttatgccg tatgatttta actatggaca cgggtaaaat cataccaaaa
      841 gatattgcgg gaaatgcagt ggctgaatct tctccattag aacataggga gagaattttg
      901 ttagcagttc gtagttatct tggagagaat attgaatgga ctaatgaaaa tgtaaattta
      961 actataaact atttaaataa cagattaaaa aaattataaa aaaattgaaa aaatggtgga
     1021 aacacttttt tcaatttttt tgttttatta tttaatattt gggaaatatt cattctaatt
     1081 ggtaatcaga ttttagaaaa caataaaccc ttgcatatga tatcgatgta cagatctttt
     1141 tttaaggcag ttattggtgc ccttcgaaat gaccgaccaa gcgacgccca acctgccatc
     1201 acgagatttc gattccaccg ccgccttcta tgaaagccat caatcctgcc tatttgccac
     1261 gtttaacaag gtagttaagc gttcatttac gaagaaaaca cgataagctg cacaaatacc
     1321 tgaaaaagtt gaacgccccg tgagcgggaa ctcacagggc gtcggctaac ccccagtcat
     1381 cagctgggag aaagcactca agacatgact ctagccgatc cgcaggacac agtcacagct
     1441 agcgcgtgga aattttccgc cgatctgttc gacacccacc ccgaactagc gctgcgctca
     1501 cgcggctgga cggcagaaga tcgccgcgaa ctgctcgctc acctgggacg cgaaagcttc
     1561 cagggcagca agacaagaga tttcgcgagc gcctggatta aaaacccgga taccggcgaa
     1621 acccaaccaa agctctaccg ggctggctca aaagcgctga cgcggtgcca gtacgttgcg
     1681 ctgacgcacg cgcaacatgc cgcggtgatc gtgcttgaca tcgatgtgcc cagccaccag
     1741 gccggcggga agattgagca cgtaaacccg caggtctacg cgattttaga gaaatgggca
     1801 cgcctagaaa aagcgccggc ttggatcggc gtgaatccgc tgagcgggaa atgccagctc
     1861 atctggctca ttgacccggt gtatgccgca gcaggtaaaa ccagcccaaa tatgcgcctg
     1921 ctggctgcaa cgacggaaga aatgactcgt gttttcggcg ctgaccaggc tttttcgcat
     1981 aggctgagcc ggtggccgct gcacgtctca gacgatccga cagcctataa atggcactgc
     2041 cagcatgatc gtgtggatcg gctggccgac ctaatggaga ttgctcgaac gatgaccgga
     2101 tcacagaagc cgaaaaagta cattgagcag gacttttcca gcggacgcgc ccgcattgaa
     2161 gcggcacaac gcgccaccgc agaagccaag gcgctagcga ttttggacgc gagcctgccg
     2221 agcgccctgg acgcgtccgg cgacctgatc gacggcgtgc gagtgctctg gacaaatcca
     2281 gagcgcgcag cgcgcgacga gaccgcgttt cgccacgcgt tgaccgtggg ataccagctc
     2341 aaagctgctg gtgagcgcct aaaagatgcc aagatcatcg acgcgtatga agtggcgtac
     2401 aacgttgccc aggcggtcgg tgcagacggc cgggagccgg atcttcccgc catgcgtgat
     2461 cgcctgacga tggcgcgtcg tgtgcgcggc tacgtggcta aaggccagcc agtcgtccct
     2521 gctcgtcggg tggaaacgca gagcagccga gggcggaaag ctctagcgac gatggggcga
     2581 cggggcgcag ctacatcgaa tgcacgcaga tgggctgacc cagaaagtaa gtatgcgcag
     2641 gagacgcgac agcgattagc ggaagcaaac aaacgccgag aaatgacagg cgagttgctc
     2701 gaacttcgcg tcaaaactgc gatcctggat gcccgttctc aatcggttgc tgatccctcg
     2761 actcgtgagc ttgcaggcga actaggtgtc agtgaaaggc gcatccaaca agtcagaaag
     2821 gcacttggaa tggaagctaa acgcggccgt ccacgggctg aaaactaata aacgaaacac
     2881 cgtcagcaga aaacggttcc cccctttagg ggtcccgtcc ttgctctggc tctcacttgc
     2941 cctcaccctc cgctatccac gggctgaaaa ctaataaacg aaacaccgtc agcagaaaac
     3001 ggttcccccc ctttagggtg tctcgctcct agctctgatc cctccccggt tcctccccgg
     3061 cctgattttt aagggggctc acgctgtcgg cagagaacgg ttcccccgcc ttctgctctg
     3121 gctcttcctc gactccctcc ccctcaaaaa tctcctcgag tctagattga cagctagctc
     3181 agtcctaggt ataatggatc cgaatttcta ctgttgtaga tcaggcaacc atagggcagg
     3241 aaatttaaat aaaacgaaag gctcagtcga aagactgggc ctttcgtttt atctgttgtt
     3301 tgtcggtgaa cgctctcctg agtaggacaa atccgccggg agcggatttg aacgttgcga
     3361 agcaacggcc cggagggtgg cgggcaggac gcccgccata aactgccagg catcaaatta
     3421 agcagaaggc catcctgacg gatggccttt ttgcgtttct acaaactctt aagcttcata
     3481 tgcggtgtga aataccgcac agatgcgtaa ggagaaaata ccgcatcagg cgctcttccg
     3541 cttcctcgct cactgactcg ctgcgctcgg tcgttcggct gcggcgagcg gtatcagctc
     3601 actcaaaggc ggtaatacgg ttatccacag aatcagggga taacgcagga aagaacatgt
     3661 gagcaaaagg ccagcaaaag gccaggaacc gtaaaaaggc cgcgttgctg gcgtttttcc
     3721 ataggctccg cccccctgac gagcatcaca aaaatcgacg ctcaagtcag aggtggcgaa
     3781 acccgacagg actataaaga taccaggcgt ttccccctgg aagctccctc gtgcgctctc
     3841 ctgttccgac cctgccgctt accggatacc tgtccgcctt tctcccttcg ggaagcgtgg
     3901 cgctttctca tagctcacgc tgtaggtatc tcagttcggt gtaggtcgtt cgctccaagc
     3961 tgggctgtgt gcacgaaccc cccgttcagc ccgaccgctg cgccttatcc ggtaactatc
     4021 gtcttgagtc caacccggta agacacgact tatcgccact ggcagcagcc actggtaaca
     4081 ggattagcag agcgaggtat gtaggcggtg ctacagagtt cttgaagtgg tggcctaact
     4141 acggctacac tagaaggaca gtatttggta tctgcgctct gctgaagcca gttaccttcg
     4201 gaaaaagagt tggtagctct tgatccggca aacaaaccac cgctggtagc ggtggttttt
     4261 ttgtttgcaa gcagcagatt acgcgcagaa aaaaaggatc tcaagaagat cctttgatct
     4321 tttctacggg gtctgacgct cagtggaacg aaaactcacg ttaagggatt ttggtcatga
//
