LOCUS       sequence-176978-        4280 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Constitutive transcription of crRNA of crtYF. CRISPR of C. 
            glutamicum..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4280)
  AUTHORS   Jiang Y, Qian F, Yang J, Liu Y, Dong F, Xu C, Sun B, Chen B, Xu X, 
            Li Y, Wang R, Yang S
  TITLE     CRISPR-Cpf1 assisted genome editing of Corynebacterium glutamicum.
  JOURNAL   Nat Commun. 2017 May 4;8:15179. doi: 10.1038/ncomms15179.
  PUBMED    28469274
REFERENCE   2  (bases 1 to 4280)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2017-05-4"; volume: "8:15179. doi"; pages: " 
            10.1038/ncomms15179"
COMMENT     Sequence Label: pJYS2.1-crtYf
FEATURES             Location/Qualifiers
     source          1..4280
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     terminator      3147..3232
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     terminator      3324..3351
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     primer_bind     complement(3445..3462)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(3616..4204)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(3696..3715)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 actaataacg taacgtgact ggcaagagat atttttaaaa caatgaatag gtttacactt
       61 actttagttt tatggaaatg aaagatcata tcatatataa tctagaataa aattaactaa
      121 aataattatt atctagataa aaaatttaga agccaatgaa atctataaat aaactaaatt
      181 aagtttattt aattaacaac tatggatata aaataggtac taatcaaaat agtgaggagg
      241 atatatttga atacatacga acaaattaat aaagtgaaaa aaatacttcg gaaacattta
      301 aaaaataacc ttattggtac ttacatgttt ggatcaggag ttgagagtgg actaaaacca
      361 aatagtgatc ttgacttttt agtcgtcgta tctgaaccat tgacagatca aagtaaagaa
      421 atacttatac aaaaaattag acctatttca aaaaaaatag gagataaaag caacttacga
      481 tatattgaat taacaattat tattcagcaa gaaatggtac cgtggaatca tcctcccaaa
      541 caagaattta tttatggaga atggttacaa gagctttatg aacaaggata cattcctcag
      601 aaggaattaa attcagattt aaccataatg ctttaccaag caaaacgaaa aaataaaaga
      661 atatacggaa attatgactt agaggaatta ctacctgata ttccattttc tgatgtgaga
      721 agagccatta tggattcgtc agaggaatta atagataatt atcaggatga tgaaaccaac
      781 tctatattaa ctttatgccg tatgatttta actatggaca cgggtaaaat cataccaaaa
      841 gatattgcgg gaaatgcagt ggctgaatct tctccattag aacataggga gagaattttg
      901 ttagcagttc gtagttatct tggagagaat attgaatgga ctaatgaaaa tgtaaattta
      961 actataaact atttaaataa cagattaaaa aaattataaa aaaattgaaa aaatggtgga
     1021 aacacttttt tcaatttttt tgttttatta tttaatattt gggaaatatt cattctaatt
     1081 ggtaatcaga ttttagaaaa caataaaccc ttgcatatga tatcgatgta cagatcccat
     1141 caatcctgcc tatttgccac gtttaacaag gtagttaagc gttcatttac gaagaaaaca
     1201 cgataagctg cacaaatacc tgaaaaagtt gaacgccccg tgagcgggaa ctcacagggc
     1261 gtcggctaac ccccagtcat cagctgggag aaagcactca agacatgact ctagccgatc
     1321 cgcaggacac agtcacagct agcgcgtgga aattttccgc cgatctgttc gacacccacc
     1381 ccgaactagc gctgcgctca cgcggctgga cggcagaaga tcgccgcgaa ctgctcgctc
     1441 acctgggacg cgaaagcttc cagggcagca agacaagaga tttcgcgagc gcctggatta
     1501 aaaacccgga taccggcgaa acccaaccaa agctctaccg ggctggctca aaagcgctga
     1561 cgcggtgcca gtacgttgcg ctgacgcacg cgcaacatgc cgcggtgatc gtgcttgaca
     1621 tcgatgtgcc cagccaccag gccggcggga agattgagca cgtaaacccg caggtctacg
     1681 cgattttaga gaaatgggca cgcctagaaa aagcgccggc ttggatcggc gtgaatccgc
     1741 tgagcgggaa atgccagctc atctggctca ttgacccggt gtatgccgca gcaggtaaaa
     1801 ccagcccaaa tatgcgcctg ctggctgcaa cgacggaaga aatgactcgt gttttcggcg
     1861 ctgaccaggc tttttcgcat aggctgagcc ggtggccgct gcacgtctca gacgatccga
     1921 cagcctataa atggcactgc cagcatgatc gtgtggatcg gctggccgac ctaatggaga
     1981 ttgctcgaac gatgaccgga tcacagaagc cgaaaaagta cattgagcag gacttttcca
     2041 gcggacgcgc ccgcattgaa gcggcacaac gcgccaccgc agaagccaag gcgctagcga
     2101 ttttggacgc gagcctgccg agcgccctgg acgcgtccgg cgacctgatc gacggcgtgc
     2161 gagtgctctg gacaaatcca gagcgcgcag cgcgcgacga gaccgcgttt cgccacgcgt
     2221 tgaccgtggg ataccagctc aaagctgctg gtgagcgcct aaaagatgcc aagatcatcg
     2281 acgcgtatga agtggcgtac aacgttgccc aggcggtcgg tgcagacggc cgggagccgg
     2341 atcttcccgc catgcgtgat cgcctgacga tggcgcgtcg tgtgcgcggc tacgtggcta
     2401 aaggccagcc agtcgtccct gctcgtcggg tggaaacgca gagcagccga gggcggaaag
     2461 ctctagcgac gatggggcga cggggcgcag ctacatcgaa tgcacgcaga tgggctgacc
     2521 cagaaagtaa gtatgcgcag gagacgcgac agcgattagc ggaagcaaac aaacgccgag
     2581 aaatgacagg cgagttgctc gaacttcgcg tcaaaactgc gatcctggat gcccgttctc
     2641 aatcggttgc tgatccctcg actcgtgagc ttgcaggcga actaggtgtc agtgaaaggc
     2701 gcatccaaca agtcagaaag gcacttggaa tggaagctaa acgcggccgt ccacgggctg
     2761 aaaactaata aacgaaacac cgtcagcaga aaacggttcc cccctttagg ggtcccgtcc
     2821 ttgctctggc tctcacttgc cctcaccctc cgctatccac gggctgaaaa ctaataaacg
     2881 aaacaccgtc agcagaaaac ggttcccccc ctttagggtg tctcgctcct agctctgatc
     2941 cctccccggt tcctccccgg cctgattttt aagggggctc acgctgtcgg cagagaacgg
     3001 ttcccccgcc ttctgctctg gctcttcctc gactccctcc ccctcaaaaa tctcctcgag
     3061 tctagattga cagctagctc agtcctaggt ataatggatc cgaatttcta ctgttgtaga
     3121 tcaggcaacc atagggcagg aaatttaaat aaaacgaaag gctcagtcga aagactgggc
     3181 ctttcgtttt atctgttgtt tgtcggtgaa cgctctcctg agtaggacaa atccgccggg
     3241 agcggatttg aacgttgcga agcaacggcc cggagggtgg cgggcaggac gcccgccata
     3301 aactgccagg catcaaatta agcagaaggc catcctgacg gatggccttt ttgcgtttct
     3361 acaaactctt aagcttcata tgcggtgtga aataccgcac agatgcgtaa ggagaaaata
     3421 ccgcatcagg cgctcttccg cttcctcgct cactgactcg ctgcgctcgg tcgttcggct
     3481 gcggcgagcg gtatcagctc actcaaaggc ggtaatacgg ttatccacag aatcagggga
     3541 taacgcagga aagaacatgt gagcaaaagg ccagcaaaag gccaggaacc gtaaaaaggc
     3601 cgcgttgctg gcgtttttcc ataggctccg cccccctgac gagcatcaca aaaatcgacg
     3661 ctcaagtcag aggtggcgaa acccgacagg actataaaga taccaggcgt ttccccctgg
     3721 aagctccctc gtgcgctctc ctgttccgac cctgccgctt accggatacc tgtccgcctt
     3781 tctcccttcg ggaagcgtgg cgctttctca tagctcacgc tgtaggtatc tcagttcggt
     3841 gtaggtcgtt cgctccaagc tgggctgtgt gcacgaaccc cccgttcagc ccgaccgctg
     3901 cgccttatcc ggtaactatc gtcttgagtc caacccggta agacacgact tatcgccact
     3961 ggcagcagcc actggtaaca ggattagcag agcgaggtat gtaggcggtg ctacagagtt
     4021 cttgaagtgg tggcctaact acggctacac tagaaggaca gtatttggta tctgcgctct
     4081 gctgaagcca gttaccttcg gaaaaagagt tggtagctct tgatccggca aacaaaccac
     4141 cgctggtagc ggtggttttt ttgtttgtaa gcagcagatt acgcgcagaa aaaaaggatc
     4201 tcaagaagat cctttgatct tttctacggg gtctgacgct cagtggaacg aaaactcacg
     4261 ttaagggatt ttggtcatga
//
