LOCUS       sequence-176984-        5811 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  For transcription of of Suv4-20h1 mRNA preceded by an HA-tag in 5'.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 5811)
  AUTHORS   Eid A, Rodriguez-Terrones D, Burton A, Torres-Padilla ME
  TITLE     SUV4-20 activity in the preimplantation mouse embryo controls timely
            replication.
  JOURNAL   Genes Dev. 2016 Nov 15;30(22):2513-2526. Epub 2016 Dec 5.
  PUBMED    27920088
REFERENCE   2  (bases 1 to 5811)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Genes 
            Dev."; date: "2016-11-15"; volume: "30(22)"; pages: "2513-2526. Epub
            2016 Dec 5"
COMMENT     Sequence Label: pRN3P-HA-Suv4-20h1
FEATURES             Location/Qualifiers
     source          1..5811
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     complement(38..57)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(39..57)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(64..81)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(64..80)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(73..95)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      complement(222..677)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(359..380)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     571..590
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     promoter        704..808
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(809..877,878..1669)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(1027..1046)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      1840..2428
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     2329..2348
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     2582..2599
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    2716..2737
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(2752..2757,2758..2775,2776..2782)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    2790..2806
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     2795..2817
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     2814..2830
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     2814..2830
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     promoter        2851..2869
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     5'UTR           2894..2936
                     /label=Xenopus globin 5'-UTR
                     /note="translational enhancer from the 5'-UTR of the major 
                     beta-globin gene of Xenopus laevis"
     regulatory      2944..2953
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             2956..2982
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     protein_bind    complement(5612..5636)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     3'UTR           5684..5809
                     /label=Xenopus globin 3'-UTR
                     /note="3'-UTR of the major beta-globin gene of Xenopus 
                     laevis"
ORIGIN
        1 cccccctgca gggccaagtc ggccggtacc caattcgccc tatagtgagt cgtattacaa
       61 ttcactggcc gtcgttttac aacgtcgtga ctgggaaaac cctggcgtta cccaacttaa
      121 tcgccttgca gcacatcccc ctttcgccag ctggcgtaat agcgaagagg cccgcaccga
      181 tcgcccttcc caacagttgc gcagcctgaa tggcgaatgg caaattgtaa gcgttaatat
      241 tttgttaaaa ttcgcgttaa atttttgtta aatcagctca ttttttaacc aataggccga
      301 aatcggcaaa atcccttata aatcaaaaga atagaccgag atagggttga gtgttgttcc
      361 agtttggaac aagagtccac tattaaagaa cgtggactcc aacgtcaaag ggcgaaaaac
      421 cgtctatcag ggcgatggcc cactacgtga accatcaccc taatcaagtt ttttggggtc
      481 gaggtgccgt aaagcactaa atcggaaccc taaagggagc ccccgattta gagcttgacg
      541 gggaaagccg gcgaacgtgg cgagaaagga agggaagaaa gcgaaaggag cgggcgctag
      601 ggcgctggca agtgtagcgg tcacgctgcg cgtaaccacc acacccgccg cgcttaatgc
      661 gccgctacag ggcgcgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg
      721 tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat
      781 gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat
      841 tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt
      901 aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag
      961 cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa
     1021 agttctgcta tgtggcgcgg tattatcccg tattgacgcc gggcaagagc aactcggtcg
     1081 ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct
     1141 tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac
     1201 tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca
     1261 caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat
     1321 accaaacgac gagcgtgaca ccacgatgcc tgtagcaatg gcaacaacgt tgcgcaaact
     1381 attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc
     1441 ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga
     1501 taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg
     1561 taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg
     1621 aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca
     1681 agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta
     1741 ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca
     1801 ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg
     1861 cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga
     1921 tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa
     1981 tactgttctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc
     2041 tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg
     2101 tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac
     2161 ggggggttcg tgcacacagc ccagcttgga gcgaacgact tacaccgaac tgagatacct
     2221 acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc
     2281 ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg
     2341 gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg
     2401 ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct
     2461 ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga
     2521 taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg
     2581 cagcgagtca gtgagcgagg aagcggaaga gcgcccaata cgcaaaccgc ctctccccgc
     2641 gcgttggccg attcattaat gcagctggca cgacaggttt cccgactgga aagcgggcag
     2701 tgagcgcaac gcaattaatg tgagttagct cactcattag gcaccccagg ctttacactt
     2761 tatgcttccg gctcgtatgt tgtgtggaat tgtgagcgga taacaatttc acacaggaaa
     2821 cagctatgac catgattacg ccaagctcga aattaaccct cactaaaggg aacaaaagct
     2881 ggagctaagc ttgcttgttc tttttgcaga agctcagaat aaacgctcaa ctttggcaga
     2941 tctgccgcca tgggctaccc atacgatgtt cctgactatg cgggatccat ggtggtgaat
     3001 ggcaggagaa atggaggcaa gttgtctaat gaccatcagc agaatcaatc aaaattacag
     3061 cagcactcgg gcaaggacac cctgaagacc ggcagaaacg ccgttgagag gcggtccagc
     3121 agatgtcatg gtaactcggg atttgaaggg cagagccgct atgtgccgtc ctctggaatg
     3181 tccgccaagg agctctgtga gaacgatgac ttagcaacca gtttggttct tgatccctac
     3241 ttaggttttc agacacacaa aatgaacact agcgcctttc cttcgaggag ctcgaggcat
     3301 atttcaaaag ctgacagttt ttctcacaac aatcctgtga gatttcggcc tataaaagga
     3361 aggcaagaag agctaaagga agtaattgaa cgctttaaga aagatgaaca cttagagaaa
     3421 gctttcaaat gtttgacttc tggggaatgg gcacggcatt attttctcaa caaaaacaaa
     3481 atgcaggaga aattattcaa ggaacatgtc tttatttact tgcggatgtt tgcaactgac
     3541 agtggatttg aaatactgcc ttgtaataga tattcttcag aacaaaatgg agccaagata
     3601 gttgcaacaa aagagtggaa acgaaatgac aaaatagaat tactggtggg ttgtattgcc
     3661 gaactttcag aaattgagga gaacatgcta cttagacacg gagaaaacga cttcagtgtc
     3721 atgtattcca caaggaaaaa ttgtgctcaa ctctggctcg gtcctgctgc atttataaat
     3781 catgattgca gacctaactg taagtttgtg tcaactggtc gagatacagc atgcgttaag
     3841 gctctgagag atattgaacc tggagaagaa atttcttgtt actatggaga tggctttttt
     3901 ggagaaaata atgagttctg cgaatgttat acttgtgaaa gacggggaac tggtgctttt
     3961 aaatcacgag taggactgcc tgcgcctgct cctgttatca atagcaaata cggacttaga
     4021 gaaacagata aacgcttaaa taggcttaaa aagttaggtg acagcagcaa aaactcagac
     4081 agtcagtctg tcagctctaa cacagatgca gacaccactc aggaaaaaga caatgcaact
     4141 tctaatcgaa aatcttcagt tggtgtgaaa aagagcagca agagtcgagc tctgacaagg
     4201 ccgtccatgc cgagagtccc ggctgcttcc aactctacct cacccaagct agtgcacacc
     4261 aacaatcccc gggtaccaaa gaaactgaga aagccggcaa agcctttact ctccaagatc
     4321 agactgcgga atcactgcaa gcggctggac cagaagagcg catcccgcaa gctcgagatg
     4381 gggagcttag tgcttaagga gcccaaagtc gtgctatata aaaatttgcc aattaagaaa
     4441 gaaagggagc cagagggacc agcccatgct gcagtgggga gtgggtgctt gactagacat
     4501 gctgcgagag aacacaggca gaatcatggg agaggtgctc attcgcaggg cgacagtttg
     4561 ccctgcacct acacaacccg gcgctctttg aggacaagga caggtctgaa ggagaccact
     4621 gacatcaagc ttgaaccaag tcccttggat ggctataaaa atggtatact ggaaccttgc
     4681 ccagacagtg gccagcagcc aaccccagag gtgctggaag aactggctcc tgagactgca
     4741 cacagggagg aagcatccca ggagtgtccc aagaacgact cctgcctgtc acgaaagaaa
     4801 tttcgacaag tgaaacctgt gaaacactta gcaaagaccg aggactgcag tccagagcac
     4861 agcttccctg ggaaagacgg gctgccagat ttgccagggt ctcatcctga tcaaggtgag
     4921 cccagtggca cagtcagggt gcccgtgagc cacacggact ctgctccctc accggttggc
     4981 tgctctgttg tcgcacccga cagcttcaca aaagacagct tcagaactgc acaaagtaaa
     5041 aagaagcggc gggtcaccag gtacgatgca cagctgatcc tggagaacag ctctggaatc
     5101 cccaagctga cgcttcgcag gcggcacgac agcagcagca agacaaacga ccatgagagt
     5161 gacggcgtga actcctccaa gatcagcatc aaactcagca aggaccacga cagtgacagc
     5221 aacctctatg ttgccaagct cagtaacggg gtcagcgcag ggccgggcag cagctccacc
     5281 aagctcaaga tccagctcaa gcgggatgag gagagcaggg ggccatgtgc agagggcctg
     5341 cacgagaacg gggtgtgctg cagcgacccc ctctccctgc tcgagtccca gatggaggtg
     5401 gacgactaca gtcaatatga ggaggacagc acagatgaat cctcatcttc tgagggggag
     5461 gaggaggagg aggactgcga ggatgacttc gatgatgact tcattcctct tcctccggca
     5521 aagcggctga ggctaattgt tggtaaagac tccatagata ttgacatttc ttcaaggaga
     5581 agagaagatc agtctctaag actgaacgca gacccagctt tcttgtacaa agtggttcga
     5641 ttcgagaatt ctaattaagc ggccgcggat ctggttacca ctaaaccagc ctcaagaaca
     5701 cccgaatgga gtctctaagc tacataatac caacttacac tttacaaaat gttgtccccc
     5761 aaaatgtagc cattcgtatc tgctcctaat aaaaagaaag tttcttcaca t
//
