LOCUS       sequence-177028-        9203 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  DSG-2 tailless control.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9203)
  AUTHORS   Baddam SR, Arsenovic PT, Narayanan V, Duggan NR, Mayer CR, Newman 
            ST, Abutaleb DA, Mohan A, Kowalczyk AP, Conway DE
  TITLE     The Desmosomal Cadherin Desmoglein-2 Experiences Mechanical Tension 
            as Demonstrated by a FRET-Based Tension Biosensor Expressed in 
            Living Cells.
  JOURNAL   Cells. 2018 Jun 26;7(7). pii: cells7070066. doi: 
            10.3390/cells7070066.
  PUBMED    29949915
REFERENCE   2  (bases 1 to 9203)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cells."; 
            date: "2018-06-26"; volume: "7(7). pii: cells7070066. doi"; pages: "
            10.3390/cells7070066"
COMMENT     Sequence Label: pcdna DSG-2 Tailless
FEATURES             Location/Qualifiers
     source          1..9203
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        136..515
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        516..719
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     670..690
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     764..783
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        764..782
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             3106..3813
                     /codon_start=1
                     /product="monomeric teal (cyan) fluorescent protein (Ai et 
                     al., 2006)"
                     /label=mTFP1
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEETTMGVIKPDMKIKLKMEGNVNGHAFVIEGEGEGKPYDG
                     TNTINLEVKEGAPLPFSYDILTTAFAYGNRAFTKYPDDIPNYFKQSFPEGYSWERTMTF
                     EDKGIVKVKSDISMEEDSFIYEIHLKGENFPPNGPVMQKKTTGWDASTERMYVRDGVLK
                     GDVKHKLLLEGGGHHRVDFKTIYRAKKAVKLPDYHFVDHRIEILNHDKDYNKVTVYESA
                     VARNSTDGMDELYK"
     CDS             join(3934..3936,3937..3939,3940..4650)
                     /codon_start=1
                     /product="Venus YFP with monomerizing A206K mutation (Nagai
                     et al., 2002; Kremers et al., 2006)"
                     /label=mVenus
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KLICTTGKLPVPWPTLVTTLGYGLQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYITADKQKNGIK
                     ANFKIRHNIEDGGVQLADHYQQNTPIGDGPVLLPDNHYLSYQSKLSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(3979..4000)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(4240..4259)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     4587..4608
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     primer_bind     complement(4695..4712)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    4701..4925
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      4971..5399
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(5058..5077)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     5268..5289
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(5408..5428)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        5413..5742
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      5593..5728
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     5655..5674
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     CDS             5809..6603
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(5863..5882)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     6473..6492
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     polyA_signal    6777..6898
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(6814..6833)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     6868..6887
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(6947..6963)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6947..6963)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(6960..6982)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    6971..6987
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(6995..7001,7002..7019,7020..7025))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7040..7061
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(7178..7195)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(7349..7937)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(7429..7448)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(8108..8899,8900..8968))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     8731..8750
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(8969..9073)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(9148..9167)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
ORIGIN
        1 tggaggtcgc tgagtagtgc gcgagcaaaa tttaagctac aacaaggcaa ggcttgaccg
       61 acaattgcat gaagaatctg cttagggtta ggcgttttgc gctgcttcgc gatgtacggg
      121 ccagatatac gcgttgacat tgattattga ctagttatta atagtaatca attacggggt
      181 cattagttca tagcccatat atggagttcc gcgttacata acttacggta aatggcccgc
      241 ctggctgacc gcccaacgac ccccgcccat tgacgtcaat aatgacgtat gttcccatag
      301 taacgccaat agggactttc cattgacgtc aatgggtgga gtatttacgg taaactgccc
      361 acttggcagt acatcaagtg tatcatatgc caagtacgcc ccctattgac gtcaatgacg
      421 gtaaatggcc cgcctggcat tatgcccagt acatgacctt atgggacttt cctacttggc
      481 agtacatcta cgtattagtc atcgctatta ccatggtgat gcggttttgg cagtacatca
      541 atgggcgtgg atagcggttt gactcacggg gatttccaag tctccacccc attgacgtca
      601 atgggagttt gttttggcac caaaatcaac gggactttcc aaaatgtcgt aacaactccg
      661 ccccattgac gcaaatgggc ggtaggcgtg tacggtggga ggtctatata agcagagctc
      721 tctggctaac tagagaaccc actgcttact ggcttatcga aattaatacg actcactata
      781 gggagaccca agctggctag cgtttaaact taagcttggt accgagctcg gatccactag
      841 tccagtgtgg tggaattctg cagatatcca gcacagtggc ggccgctcga gggtgcgatg
      901 gcgcggagcc cgggacgcgc gtacgccctg ctgcttctcc tgatctgctt taacgttgga
      961 agtggacttc acttacaggt cttaagcaca agaaatgaaa ataagctgct tcctaaacat
     1021 cctcatttag tgcggcaaaa gcgcgcctgg atcaccgccc ccgtggctct tcgggaggga
     1081 gaggatctgt ccaagaagaa tccaattgcc aagatacatt ctgatcttgc agaagaaaga
     1141 ggactcaaaa ttacttacaa atacactgga aaagggatta cagagccacc ttttggtata
     1201 tttgtcttta acaaagatac tggagaactg aatgttacca gcattcttga tcgagaagaa
     1261 acaccatttt ttctgctaac aggttacgct ttggatgcaa gaggaaacaa tgtagagaaa
     1321 cccttagagc tacgcattaa ggttcttgat atcaatgaca acgaaccagt gttcacacag
     1381 gatgtctttg ttgggtctgt tgaagagttg agtgcagcac atactcttgt gatgaaaatc
     1441 aatgcaacag atgcagatga gcccaatacc ctgaattcga aaatttccta tagaatcgta
     1501 tctctggagc ctgcttatcc tccagtgttc tacctaaata aagatacagg agagatttat
     1561 acaaccagtg ttaccttgga cagagaggaa cacagcagct acactttgac agtagaagca
     1621 agagatggca atggagaagt tacagacaaa cctgtaaaac aagctcaagt tcagattcgt
     1681 attttggatg tcaatgacaa tatacctgta gtagaaaata aagtgcttga agggatggtt
     1741 gaagaaaatc aagtcaatgt agaagttacg cgcataaaag tgttcgatgc agatgaaata
     1801 ggttctgata attggctggc aaattttaca tttgcatcag gaaatgaagg aggttatttc
     1861 cacatagaaa cagatgctca aactaacgaa ggaattgtga cccttattaa ggaagtagat
     1921 tatgaagaaa tgaagaatct tgacttcagt gttattgtcg ctaataaagc agcttttcac
     1981 aagtcgatta ggagtaaata caagcctaca cccattccca tcaaggtcaa agtgaaaaat
     2041 gtgaaagaag gcattcattt taaaagcagc gtcatctcaa tttatgttag cgagagcatg
     2101 gatagatcaa gcaaaggcca aataattgga aattttcaag cttttgatga ggacactgga
     2161 ctaccagccc atgcaagata tgtaaaatta gaagatagag ataattggat ctctgtggat
     2221 tctgtcacat ctgaaattaa acttgcaaaa cttcctgatt ttgaatctag atatgttcaa
     2281 aatggcacat acactgtaaa gattgtggcc atatcagaag attatcctag aaaaaccatc
     2341 actggcacag tccttatcaa tgttgaagac atcaacgaca actgtcccac actgatagag
     2401 cctgtgcaga caatctgtca cgatgcagag tatgtgaatg ttactgcaga ggacctggat
     2461 ggacacccaa acagtggccc tttcagtttc tccgtcattg acaaaccacc tggcatggca
     2521 gaaaaatgga aaatagcacg ccaagaaagt accagtgtgc tgctgcaaca aagtgagaaa
     2581 aagcttggga gaagtgaaat tcagttcctg atttcagaca atcagggttt tagttgtcct
     2641 gaaaagcagg tccttacact cacagtttgt gagtgtctgc atggcagcgg ctgcagggaa
     2701 gcacagcatg actcctatgt gggcctggga cccgcagcaa ttgcgctcat gattttggcc
     2761 tttctgctcc tgctattggt accactttta ctgctgatgt gccattgcgg aaagggcgcc
     2821 aaaggcttta cccccatacc tggcaccata gagatgctgc atccttggaa taatgaagga
     2881 gcaccacctg aagacaaggt ggtgccatca tttctgccag tggatcaagg gggcagtcta
     2941 gtaggaagaa atggagtagg aggtatggcc aaggaagcca cgatgaaagg aagtagctct
     3001 gcttccattg tcaaagggca acatgagatg tccgagatgg atggaaggtg ggaagaacac
     3061 agaagcctgc tttctggtag agctacccag tttacagggg tcgagatggt gagcaagggc
     3121 gaggagacca caatgggcgt aatcaagccc gacatgaaga tcaagctgaa gatggagggc
     3181 aacgtgaatg gccacgcctt cgtgatcgag ggcgagggcg agggcaagcc ctacgacggc
     3241 accaacacca tcaacctgga ggtgaaggag ggagcccccc tgcccttctc ctacgacatt
     3301 ctgaccaccg cgttcgccta cggcaacagg gccttcacca agtaccccga cgacatcccc
     3361 aactacttca agcagtcctt ccccgagggc tactcttggg agcgcaccat gaccttcgag
     3421 gacaagggca tcgtgaaggt gaagtccgac atctccatgg aggaggactc cttcatctac
     3481 gagatacacc tcaagggcga gaacttcccc cccaacggcc ccgtgatgca gaagaagacc
     3541 accggctggg acgcctccac cgagaggatg tacgtgcgcg acggcgtgct gaagggcgac
     3601 gtcaagcaca agctgctgct ggagggcggc ggccaccacc gcgttgactt caagaccatc
     3661 tacagggcca agaaggcggt gaagctgccc gactatcact ttgtggacca ccgcatcgag
     3721 atcctgaacc acgacaagga ctacaacaag gtgaccgttt acgagagcgc cgtggcccgc
     3781 aactccaccg acggcatgga cgagctgtac aaggggccag gtggtgcagg gccaggtggt
     3841 gcagggccag gtggtgcagg gccaggtggt gcagggcccg gtggtgcagg tccaggtggt
     3901 gcaggtccag gtggtgcagg tccaggtggt gctatggtga gcaagggcga ggagctgttc
     3961 accggggtgg tgcccatcct ggtcgagctg gacggcgacg taaacggcca caagttcagc
     4021 gtgtccggcg agggcgaggg cgatgccacc tacggcaagc tgaccctgaa gctgatctgc
     4081 accaccggca agctgcccgt gccctggccc accctcgtga ccaccctggg ctacggcctg
     4141 cagtgcttcg cccgctaccc cgaccacatg aagcagcacg acttcttcaa gtccgccatg
     4201 cccgaaggct acgtccagga gcgcaccatc ttcttcaagg acgacggcaa ctacaagacc
     4261 cgcgccgagg tgaagttcga gggcgacacc ctggtgaacc gcatcgagct gaagggcatc
     4321 gacttcaagg aggacggcaa catcctgggg cacaagctgg agtacaacta caacagccac
     4381 aacgtctata tcaccgccga caagcagaag aacggcatca aggccaactt caagatccgc
     4441 cacaacatcg aggacggcgg cgtgcagctc gccgaccact accagcagaa cacccccatc
     4501 ggcgacggcc ccgtgctgct gcccgacaac cactacctga gctaccagtc caaactgagc
     4561 aaagacccca acgagaagcg cgatcacatg gtcctgctgg agttcgtgac cgccgccggg
     4621 atcactctcg gcatggacga gctgtacaag gcggccgctc gagtctagag ggcccgttta
     4681 aacccgctga tcagcctcga ctgtgccttc tagttgccag ccatctgttg tttgcccctc
     4741 ccccgtgcct tccttgaccc tggaaggtgc cactcccact gtcctttcct aataaaatga
     4801 ggaaattgca tcgcattgtc tgagtaggtg tcattctatt ctggggggtg gggtggggca
     4861 ggacagcaag ggggaggatt gggaagacaa tagcaggcat gctggggatg cggtgggctc
     4921 tatggcttct gaggcggaaa gaaccagctg gggctctagg gggtatcccc acgcgccctg
     4981 tagcggcgca ttaagcgcgg cgggtgtggt ggttacgcgc agcgtgaccg ctacacttgc
     5041 cagcgcccta gcgcccgctc ctttcgcttt cttcccttcc tttctcgcca cgttcgccgg
     5101 ctttccccgt caagctctaa atcgggggct ccctttaggg ttccgattta gtgctttacg
     5161 gcacctcgac cccaaaaaac ttgattaggg tgatggttca cgtagtgggc catcgccctg
     5221 atagacggtt tttcgccctt tgacgttgga gtccacgttc tttaatagtg gactcttgtt
     5281 ccaaactgga acaacactca accctatctc ggtctattct tttgatttat aagggatttt
     5341 gccgatttcg gcctattggt taaaaaatga gctgatttaa caaaaattta acgcgaatta
     5401 attctgtgga atgtgtgtca gttagggtgt ggaaagtccc caggctcccc agcaggcaga
     5461 agtatgcaaa gcatgcatct caattagtca gcaaccaggt gtggaaagtc cccaggctcc
     5521 ccagcaggca gaagtatgca aagcatgcat ctcaattagt cagcaaccat agtcccgccc
     5581 ctaactccgc ccatcccgcc cctaactccg cccagttccg cccattctcc gccccatggc
     5641 tgactaattt tttttattta tgcagaggcc gaggccgcct ctgcctctga gctattccag
     5701 aagtagtgag gaggcttttt tggaggccta ggcttttgca aaaagctccc gggagcttgt
     5761 atatccattt tcggatctga tcaagagaca ggatgaggat cgtttcgcat gattgaacaa
     5821 gatggattgc acgcaggttc tccggccgct tgggtggaga ggctattcgg ctatgactgg
     5881 gcacaacaga caatcggctg ctctgatgcc gccgtgttcc ggctgtcagc gcaggggcgc
     5941 ccggttcttt ttgtcaagac cgacctgtcc ggtgccctga atgaactgca ggacgaggca
     6001 gcgcggctat cgtggctggc cacgacgggc gttccttgcg cagctgtgct cgacgttgtc
     6061 actgaagcgg gaagggactg gctgctattg ggcgaagtgc cggggcagga tctcctgtca
     6121 tctcaccttg ctcctgccga gaaagtatcc atcatggctg atgcaatgcg gcggctgcat
     6181 acgcttgatc cggctacctg cccattcgac caccaagcga aacatcgcat cgagcgagca
     6241 cgtactcgga tggaagccgg tcttgtcgat caggatgatc tggacgaaga gcatcagggg
     6301 ctcgcgccag ccgaactgtt cgccaggctc aaggcgcgca tgcccgacgg cgaggatctc
     6361 gtcgtgaccc atggcgatgc ctgcttgccg aatatcatgg tggaaaatgg ccgcttttct
     6421 ggattcatcg actgtggccg gctgggtgtg gcggaccgct atcaggacat agcgttggct
     6481 acccgtgata ttgctgaaga gcttggcggc gaatgggctg accgcttcct cgtgctttac
     6541 ggtatcgccg ctcccgattc gcagcgcatc gccttctatc gccttcttga cgagttcttc
     6601 tgagcgggac tctggggttc gaaatgaccg accaagcgac gcccaacctg ccatcacgag
     6661 atttcgattc caccgccgcc ttctatgaaa ggttgggctt cggaatcgtt ttccgggacg
     6721 ccggctggat gatcctccag cgcggggatc tcatgctgga gttcttcgcc caccccaact
     6781 tgtttattgc agcttataat ggttacaaat aaagcaatag catcacaaat ttcacaaata
     6841 aagcattttt ttcactgcat tctagttgtg gtttgtccaa actcatcaat gtatcttatc
     6901 atgtctgtat accgtcgacc tctagctaga gcttggcgta atcatggtca tagctgtttc
     6961 ctgtgtgaaa ttgttatccg ctcacaattc cacacaacat acgagccgga agcataaagt
     7021 gtaaagcctg gggtgcctaa tgagtgagct aactcacatt aattgcgttg cgctcactgc
     7081 ccgctttcca gtcgggaaac ctgtcgtgcc agctgcatta atgaatcggc caacgcgcgg
     7141 ggagaggcgg tttgcgtatt gggcgctctt ccgcttcctc gctcactgac tcgctgcgct
     7201 cggtcgttcg gctgcggcga gcggtatcag ctcactcaaa ggcggtaata cggttatcca
     7261 cagaatcagg ggataacgca ggaaagaaca tgtgagcaaa aggccagcaa aaggccagga
     7321 accgtaaaaa ggccgcgttg ctggcgtttt tccataggct ccgcccccct gacgagcatc
     7381 acaaaaatcg acgctcaagt cagaggtggc gaaacccgac aggactataa agataccagg
     7441 cgtttccccc tggaagctcc ctcgtgcgct ctcctgttcc gaccctgccg cttaccggat
     7501 acctgtccgc ctttctccct tcgggaagcg tggcgctttc tcatagctca cgctgtaggt
     7561 atctcagttc ggtgtaggtc gttcgctcca agctgggctg tgtgcacgaa ccccccgttc
     7621 agcccgaccg ctgcgcctta tccggtaact atcgtcttga gtccaacccg gtaagacacg
     7681 acttatcgcc actggcagca gccactggta acaggattag cagagcgagg tatgtaggcg
     7741 gtgctacaga gttcttgaag tggtggccta actacggcta cactagaaga acagtatttg
     7801 gtatctgcgc tctgctgaag ccagttacct tcggaaaaag agttggtagc tcttgatccg
     7861 gcaaacaaac caccgctggt agcggtggtt tttttgtttg caagcagcag attacgcgca
     7921 gaaaaaaagg atctcaagaa gatcctttga tcttttctac ggggtctgac gctcagtgga
     7981 acgaaaactc acgttaaggg attttggtca tgagattatc aaaaaggatc ttcacctaga
     8041 tccttttaaa ttaaaaatga agttttaaat caatctaaag tatatatgag taaacttggt
     8101 ctgacagtta ccaatgctta atcagtgagg cacctatctc agcgatctgt ctatttcgtt
     8161 catccatagt tgcctgactc cccgtcgtgt agataactac gatacgggag ggcttaccat
     8221 ctggccccag tgctgcaatg ataccgcgag acccacgctc accggctcca gatttatcag
     8281 caataaacca gccagccgga agggccgagc gcagaagtgg tcctgcaact ttatccgcct
     8341 ccatccagtc tattaattgt tgccgggaag ctagagtaag tagttcgcca gttaatagtt
     8401 tgcgcaacgt tgttgccatt gctacaggca tcgtggtgtc acgctcgtcg tttggtatgg
     8461 cttcattcag ctccggttcc caacgatcaa ggcgagttac atgatccccc atgttgtgca
     8521 aaaaagcggt tagctccttc ggtcctccga tcgttgtcag aagtaagttg gccgcagtgt
     8581 tatcactcat ggttatggca gcactgcata attctcttac tgtcatgcca tccgtaagat
     8641 gcttttctgt gactggtgag tactcaacca agtcattctg agaatagtgt atgcggcgac
     8701 cgagttgctc ttgcccggcg tcaatacggg ataataccgc gccacatagc agaactttaa
     8761 aagtgctcat cattggaaaa cgttcttcgg ggcgaaaact ctcaaggatc ttaccgctgt
     8821 tgagatccag ttcgatgtaa cccactcgtg cacccaactg atcttcagca tcttttactt
     8881 tcaccagcgt ttctgggtga gcaaaaacag gaaggcaaaa tgccgcaaaa aagggaataa
     8941 gggcgacacg gaaatgttga atactcatac tcttcctttt tcaatattat tgaagcattt
     9001 atcagggtta ttgtctcatg agcggataca tatttgaatg tatttagaaa aataaacaaa
     9061 taggggttcc gcgcacattt ccccgaaaag tgccacctga cgtcgacgga tcgggagatc
     9121 tcccgatccc ctatggtgca ctctcagtac aatctgctct gatgccgcat agttaagcca
     9181 gtatctgctc cctgcttgtg tgt
//
