LOCUS       sequence-177050-        7145 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  retroviral vector encoding cre dependent expression of membrane 
            Tdtomato.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7145)
  AUTHORS   Lim L, Pakan JMP, Selten MM, Marques-Smith A, Llorca A, Bae SE, 
            Rochefort NL, Marin O
  TITLE     Optimization of interneuron function by direct coupling of cell 
            migration and axonal targeting.
  JOURNAL   Nat Neurosci. 2018 Jun 18. pii: 10.1038/s41593-018-0162-9. doi: 
            10.1038/s41593-018-0162-9.
  PUBMED    29915195
REFERENCE   2  (bases 1 to 7145)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Neurosci. 2018 Jun 18. pii: 10.1038/s41593-018-0162-9. doi: 
            10.1038/s41593-018-0162-9."
COMMENT     Sequence Label: RV-Syn-Dio-mTdt
FEATURES             Location/Qualifiers
     source          1..7145
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             join(18..86,87..878)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(236..255)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      1038..1626
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     1527..1546
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     1780..1797
                     /label=L4440
                     /note="L4440 vector, forward primer"
     misc_feature    2668..3256
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     CDS             2774..2785
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     3183..3203
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     protein_bind    complement(3266..3299)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     protein_bind    complement(3350..3383)
                     /label=lox2272
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (AAGTATCC) (Shaw et al., 2021). lox2272 sites are 
                     compatible with each other, but incompatible with loxP or 
                     loxN sites (Lee and Saito, 1988)."
     CDS             3455..4885
                     /codon_start=1
                     /product="tandem dimeric (pseudo-monomeric) derivative of 
                     DsRed (Shaner et al., 2004)"
                     /label=tdTomato
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEVIKEFMRFKVRMEGSMNGHEFEIEGEGEGRPYEGTQTA
                     KLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYKKLSFPEGFKWERVMNFEDGG
                     LVTVTQDSSLQDGTLIYKVKMRGTNFPPDGPVMQKKTMGWEASTERLYPRDGVLKGEIH
                     QALKLKDGGHYLVEFKTIYMAKKPVQLPGYYYVDTKLDITSHNEDYTIVEQYERSEGRH
                     HLFLGHGTGSTGSGSSGTASSEDNNMAVIKEFMRFKVRMEGSMNGHEFEIEGEGEGRPY
                     EGTQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYKKLSFPEGFKWERVM
                     NFEDGGLVTVTQDSSLQDGTLIYKVKMRGTNFPPDGPVMQKKTMGWEASTERLYPRDGV
                     LKGEIHQALKLKDGGHYLVEFKTIYMAKKPVQLPGYYYVDTKLDITSHNEDYTIVEQYE
                     RSEGRHHLFLYGMDELYK"
     protein_bind    4894..4927
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     protein_bind    4978..5011
                     /label=lox2272
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (AAGTATCC) (Shaw et al., 2021). lox2272 sites are 
                     compatible with each other, but incompatible with loxP or 
                     loxN sites (Lee and Saito, 1988)."
     CDS             complement(5373..5789)
                     /label=gag (truncated)
                     /note="truncated Moloney murine leukemia virus (MMLV) gag 
                     gene lacking the start codon"
     primer_bind     complement(5389..5405)
                     /label=pBABE 5'
                     /note="Psi packaging signal, forward primer for pBABE 
                     vectors"
     primer_bind     complement(5421..5443)
                     /label=pLXSN 5'
                     /note="Murine stem cell virus, forward primer. Also called 
                     MSCV"
     misc_feature    5990..6189
                     /label=MMLV Psi
                     /note="packaging signal of Moloney murine leukemia virus 
                     (MMLV)"
     LTR             6252..6427
                     /label=5' LTR (truncated)
                     /note="truncated long terminal repeat from Moloney murine 
                     sarcoma virus"
     promoter        complement(6428..6631)
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     complement(6457..6477)
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     enhancer        6632..7011
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
ORIGIN
        1 attgaaaaag gaagagtatg agtattcaac atttccgtgt cgcccttatt cccttttttg
       61 cggcattttg ccttcctgtt tttgctcacc cagaaacgct ggtgaaagta aaagatgctg
      121 aagatcagtt gggtgcacga gtgggttaca tcgaactgga tctcaacagc ggtaagatcc
      181 ttgagagttt tcgccccgaa gaacgttttc caatgatgag cacttttaaa gttctgctat
      241 gtggcgcggt attatcccgt attgacgccg ggcaagagca actcggtcgc cgcatacact
      301 attctcagaa tgacttggtt gagtactcac cagtcacaga aaagcatctt acggatggca
      361 tgacagtaag agaattatgc agtgctgcca taaccatgag tgataacact gcggccaact
      421 tacttctgac aacgatcgga ggaccgaagg agctaaccgc ttttttgcac aacatggggg
      481 atcatgtaac tcgccttgat cgttgggaac cggagctgaa tgaagccata ccaaacgacg
      541 agcgtgacac cacgatgcct gtagcaatgg caacaacgtt gcgcaaacta ttaactggcg
      601 aactacttac tctagcttcc cggcaacaat taatagactg gatggaggcg gataaagttg
      661 caggaccact tctgcgctcg gcccttccgg ctggctggtt tattgctgat aaatctggag
      721 ccggtgagcg tgggtctcgc ggtatcattg cagcactggg gccagatggt aagccctccc
      781 gtatcgtagt tatctacacg acggggagtc aggcaactat ggatgaacga aatagacaga
      841 tcgctgagat aggtgcctca ctgattaagc attggtaact gtcagaccaa gtttactcat
      901 atatacttta gattgatttg cggccgcaaa aggatctagg tgaagatcct ttttgataat
      961 ctcatgacca aaatccctta acgtgagttt tcgttccact gagcgtcaga ccccgtagaa
     1021 aagatcaaag gatcttcttg agatcctttt tttctgcgcg taatctgctg cttgcaaaca
     1081 aaaaaaccac cgctaccagc ggtggtttgt ttgccggatc aagagctacc aactcttttt
     1141 ccgaaggtaa ctggcttcag cagagcgcag ataccaaata ctgttcttct agtgtagccg
     1201 tagttaggcc accacttcaa gaactctgta gcaccgccta catacctcgc tctgctaatc
     1261 ctgttaccag tggctgctgc cagtggcgat aagtcgtgtc ttaccgggtt ggactcaaga
     1321 cgatagttac cggataaggc gcagcggtcg ggctgaacgg ggggttcgtg cacacagccc
     1381 agcttggagc gaacgaccta caccgaactg agatacctac agcgtgagct atgagaaagc
     1441 gccacgcttc ccgaagggag aaaggcggac aggtatccgg taagcggcag ggtcggaaca
     1501 ggagagcgca cgagggagct tccaggggga aacgcctggt atctttatag tcctgtcggg
     1561 tttcgccacc tctgacttga gcgtcgattt ttgtgatgct cgtcaggggg gcggagccta
     1621 tggaaaaacg ccagcaacgc ggccttttta cggttcctgg ccttttgctg gccttttgct
     1681 cacatgttct ttcctgcgtt atcccctgat tctgtggata accgtattac cgcctttgag
     1741 tgagctgata ccgctcgccg cagccgaacg accgagcgca gcgagtcagt gagcgaggaa
     1801 gcggaagagc gcccaatacg caaaccgcct ctccccgcgc gttggccgat tcattaatgc
     1861 aactatggcc atttaatgta aatacttaag aaaaaaaacc aaattaattt tgatacatgc
     1921 tgcatgtgaa agacccccgc tgacgggtag tcaatcactc agaggagacc ctcccaagga
     1981 acagcgagac cacaagtcgg atgygaaaga cccccgctga cgggtagtca atcactcaga
     2041 ggagaccctc ccaaggaaca gcgagaccac aagtcggatg caactgcaag agggtttatt
     2101 ggatacacgg gtacccgggc gactcagtca atcggaggac tggcgccccg agtgaggggt
     2161 tgtgggctct tttattgagc tcggggagca gaagcgcgcg aacagaagcg agaagcgaac
     2221 tgattggtta gttcaaataa ggcacagggt catttcaggt ccttggggca ccctggaaac
     2281 atctgatggt tctctagaaa ctgctgaggg ctggaccgca tctggggacc atctgttctt
     2341 ggccctgagc cggggcagga actgcttacc acagatatcc tgtttggccc atattcagct
     2401 gttccatctg ttcttggccc tgagccgggg caggaactgc ttaccacaga tatcctgttt
     2461 ggcccatatt cagctgttcc atctgttcct gaccttgatc tgaacttctc tattctcagt
     2521 tatgtatttt tccatgcctt gcaaaatggc gttacttaag ctagcttgcc aaacctacag
     2581 gtggggtctt tcattccccc ctttttctgg agactaaata aaatctttta ttttatcgat
     2641 gttaacaagc tcgagctcga attccaggcg gggaggcggc ccaaagggag atccgactcg
     2701 tctgagggcg aaggcgaaga cgcggaagag gccgcagagc cggcagcagg ccgcgggaag
     2761 gaaggtccgc tggattgagg gccgaaggga cgtagcagaa ggacgtcccg cgcagaatcc
     2821 aggtggcaac acaggcgagc agccatggaa aggacgtcag cttccccgac aacaccacgg
     2881 aattgtcagt gcccaacagc cgagcccctg tccagcagcg ggcaaggcag gcggcgatga
     2941 gttccgccgt ggcaataggg agggggaaag cgaaagtccc ggaaaggagc tgacaggtgg
     3001 tggcaatgcc ccaaccagtg ggggttgcgt cagcaaacac agtgcacacc acgccacgtt
     3061 gcctgacaac gggccacaac tcctcataaa gagacagcaa ccaggattta tacaaggagg
     3121 agaaaatgaa agccatacgg gaagcaatag catgatacaa aggcattaaa gcagcgtatc
     3181 cacatagcgt aaaaggagca acatagttaa gaataccagt caatctttca caaattttgt
     3241 aatccagagg ttgattggtt taaacataac ttcgtatagc atacattata cgaagttatt
     3301 ctttgcacca ttctaaagaa taacagtgat aatttctggg ttaaggcaaa taacttcgta
     3361 taggatactt tatacgaagt tatcgcggcg cgccatgctg tgctgtatga gaagaaccaa
     3421 acaggttgaa aagaatgatg aggaccaaaa gatcatggtg agcaagggcg aggaggtcat
     3481 caaagagttc atgcgcttca aggtgcgcat ggagggctcc atgaacggcc acgagttcga
     3541 gatcgagggc gagggcgagg gccgccccta cgagggcacc cagaccgcca agctgaaggt
     3601 gaccaagggc ggccccctgc ccttcgcctg ggacatcctg tccccccagt tcatgtacgg
     3661 ctccaaggcg tacgtgaagc accccgccga catccccgat tacaagaagc tgtccttccc
     3721 cgagggcttc aagtgggagc gcgtgatgaa cttcgaggac ggcggtctgg tgaccgtgac
     3781 ccaggactcc tccctgcagg acggcacgct gatctacaag gtgaagatgc gcggcaccaa
     3841 cttccccccc gacggccccg taatgcagaa gaagaccatg ggctgggagg cctccaccga
     3901 gcgcctgtac ccccgcgacg gcgtgctgaa gggcgagatc caccaggccc tgaagctgaa
     3961 ggacggcggc cactacctgg tggagttcaa gaccatctac atggccaaga agcccgtgca
     4021 actgcccggc tactactacg tggacaccaa gctggacatc acctcccaca acgaggacta
     4081 caccatcgtg gaacagtacg agcgctccga gggccgccac cacctgttcc tggggcatgg
     4141 caccggcagc accggcagcg gcagctccgg caccgcctcc tccgaggaca acaacatggc
     4201 cgtcatcaaa gagttcatgc gcttcaaggt gcgcatggag ggctccatga acggccacga
     4261 gttcgagatc gagggcgagg gcgagggccg cccctacgag ggcacccaga ccgccaagct
     4321 gaaggtgacc aagggcggcc ccctgccctt cgcctgggac atcctgtccc cccagttcat
     4381 gtacggctcc aaggcgtacg tgaagcaccc cgccgacatc cccgattaca agaagctgtc
     4441 cttccccgag ggcttcaagt gggagcgcgt gatgaacttc gaggacggcg gtctggtgac
     4501 cgtgacccag gactcctccc tgcaggacgg cacgctgatc tacaaggtga agatgcgcgg
     4561 caccaacttc ccccccgacg gccccgtaat gcagaagaag accatgggct gggaggcctc
     4621 caccgagcgc ctgtaccccc gcgacggcgt gctgaagggc gagatccacc aggccctgaa
     4681 gctgaaggac ggcggccact acctggtgga gttcaagacc atctacatgg ccaagaagcc
     4741 cgtgcaactg cccggctact actacgtgga caccaagctg gacatcacct cccacaacga
     4801 ggactacacc atcgtggaac agtacgagcg ctccgagggc cgccaccacc tgttcctgta
     4861 cggcatggac gagctgtaca agtaattaat taaataactt cgtataatgt atgctatacg
     4921 aagttattaa gaggtttcat attgctaata gcagctacaa tccagctacc attctgcata
     4981 acttcgtata aagtatccta tacgaagtta ttccggatcc aaggggcagt gggtcggtgg
     5041 gccgcggggg cggagcgcaa ggggctcagg agcacggcgg cgctctctgg cacgacgcga
     5101 ctcctctgct gcccgctgca gactgaggca gcgctgaggc tctgccgccg cagagcgtat
     5161 ggtcgcgcct gcacccccct atcttgcacc tcttgtggtg cggcccgact ggactcaccg
     5221 ctgcacggac gcgaccaagg cggtgaggta ggggagtttg gggagggctg gagagtgacg
     5281 tcagcccgcc tttggtgctg gggaggtggt ggcgcgcgcc ggcagcgggg gcgaaggctc
     5341 tgtccgcggt actaaagtcg acatcgaatt ccggcgccta gagaaggagt gagggctgga
     5401 taaagggagg atcgaggcgg ggtcgaacga ggaggttcaa gggggagaga cggggcggat
     5461 ggaggaagag gaggcggagg cttagggtgt acaaagggct tgacccaggg aggggggtca
     5521 aaagccaagg cttcccaggt cacgatgtag gggacctggt ctgggtgtcc atgcgggcca
     5581 ggtgaaaaga ccttgatctt aacctgggtg atgaggtctc ggttaaaggt gccgtctcgc
     5641 ggccatccga cgttaaaggt tggccattct gcagagcaga aggtaaccca acgtctcttc
     5701 ttgacatcta ccgactggtt gtgagcgatc cgctcgacat ctttccagtg acctaaggtc
     5761 aaacttaagg gagtggtaac agtctggccc taattttcag acaaatacag aaacacagtc
     5821 agacagagac aacacagaac gatgctgcag cgctgcagca gacaagacgc gcggcttcgg
     5881 ttccaaaccg aaagcaaaaa ttcagacgga ggcgggaact gttttaggtt ctcgtctcct
     5941 accagaacca catatcctga cggggtcgga ttccacatcg actcccttcc tcaggtcggg
     6001 ccacaaaaac ggcccccaaa gtccctggga cgtctcccag ggttgcggcc gggtgttcag
     6061 aactcgtcag ttccaccacg ggtccgccag atacagagct agttagctaa ctagtacaga
     6121 cgcaggcgca taacatcaaa catagacact agacaatcgg acagacacag ataagttgct
     6181 ggccagctta cctcccggtg gtgggtcggt ggtccctggg caggggtctc caaatcccgg
     6241 acgagccccc aaatgaaaga cccccgtcgt gggtagtcaa tcactcagag gagaccctcc
     6301 caaggaacag cgagaccacg attcggatgc aaacagcaag aggctttatt gggaatacgg
     6361 gtacccgggc gactcagtca atcggaggac tggcgccccg agtgaggggt tgtgggctct
     6421 tttattgagc tctgcttata tagacctccc accgtacacg cctaccgccc atttgcgtca
     6481 atggggcgga gttgttacga cattttggaa agtcccgttg attttggtgc caaaacaaac
     6541 tcccattgac gtcaatgggg tggagacttg gaaatccccg tgagtcaaac cgctatccac
     6601 gcccattgat gtactgccaa aaccgcatca ccatggtaat agcgatgact aatacgtaga
     6661 tgtactgcca agtaggaaag tcccataagg tcatgtactg ggcataatgc caggcgggcc
     6721 atttaccgtc attgacgtca atagggggcg tacttggcat atgatacact tgatgtactg
     6781 ccaagtgggc agtttaccgt aaatactcca cccattgacg tcaatggaaa gtccctattg
     6841 gcgttactat gggaacatac gtcattattg acgtcaatgg gcgggggtcg ttgggcggtc
     6901 agccaggcgg gccatttacc gtaagttatg taacgcggaa ctccatatat gggctatgaa
     6961 ctaatgaccc cgtaattgat tactattaat aactagtcaa taatcaatgt caacatggcg
     7021 gtaatgttgg acatgagcca atataaatgt acatattatg atatggatac aacgtatgca
     7081 atggccaata gccaatattg atttatgcta tataaccaat gaataatatg gctaatggcc
     7141 ggatc
//
