LOCUS       sequence-177053-       10120 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  GFP-tagged BRD protein, which can be used to be expressed in 
            mammalian cells..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10120)
  AUTHORS   Gong F, Chiu LY, Cox B, Aymard F, Clouaire T, Leung JW, Cammarata M,
            Perez M, Agarwal P, Brodbelt JS, Legube G, Miller KM
  TITLE     Screen identifies bromodomain protein ZMYND8 in chromatin 
            recognition of transcription-associated DNA damage that promotes 
            homologous recombination.
  JOURNAL   Genes Dev. 2015 Jan 15;29(2):197-211. doi: 10.1101/gad.252189.114.
  PUBMED    25593309
REFERENCE   2  (bases 1 to 10120)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1101/gad.252189"; journalName: "Genes Dev"; date: "2015-01-15- 
            15"; volume: "29"; issue: "2"; pages: "197-211"
COMMENT     Sequence Label: GFP-ATAD2
FEATURES             Location/Qualifiers
     source          1..10120
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        94..473
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        474..677
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     628..648
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     674..698
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     CDS             join(802..804,805..807,808..1518)
                     /codon_start=1
                     /product="Emerald GFP"
                     /label=EmGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTFTYGVQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHKVYITADKQKNGIK
                     VNFKTRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(847..868)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(1108..1127)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     1455..1476
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     protein_bind    1534..1558
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             complement(2414..2437)
                     /codon_start=1
                     /product="8xHis affinity tag"
                     /label=8xHis
                     /translation="HHHHHHHH"
     protein_bind    complement(5804..5828)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             5836..5877
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     primer_bind     complement(5917..5936)
                     /label=TK-pA-R
                     /note="Thymidine kinase polyA, reverse primer"
     polyA_signal    5961..6009
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase 
                     polyadenylation signal (Cole and Stacy, 1985)"
     rep_origin      6211..6639
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(6298..6317)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     6508..6529
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(6648..6668)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        6653..6982
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      6833..6968
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     6895..6914
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     promoter        7030..7077
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     CDS             7096..7494
                     /codon_start=1
                     /gene="Aspergillus terreus BSD"
                     /product="blasticidin S deaminase"
                     /label=BSD
                     /note="confers resistance to blasticidin"
                     /translation="MAKPLSQEESTLIERATATINSIPISEDYSVASAALSSDGRIFTG
                     VNVYHFTGGPCAELVVLGTAAAAAAGNLTCIVAIGNENRGILSPCGRCRQVLLDLHPGI
                     KAIVKDSDGQPTAVGIRELLPSGYVWEG"
     polyA_signal    7652..7773
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(7689..7708)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     7743..7762
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(7822..7838)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(7822..7838)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(7835..7857)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    7846..7862
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(7870..7876,7877..7894,7895..7900))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7915..7936
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(8053..8070)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(8224..8812)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(8304..8323)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(8983..9774,9775..9843))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     9606..9625
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(9844..9948)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(10023..10042)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
ORIGIN
        1 caaggcaagg cttgaccgac aattgcatga agaatctgct tagggttagg cgttttgcgc
       61 tgcttcgcga tgtacgggcc agatatacgc gttgacattg attattgact agttattaat
      121 agtaatcaat tacggggtca ttagttcata gcccatatat ggagttccgc gttacataac
      181 ttacggtaaa tggcccgcct ggctgaccgc ccaacgaccc ccgcccattg acgtcaataa
      241 tgacgtatgt tcccatagta acgccaatag ggactttcca ttgacgtcaa tgggtggagt
      301 atttacggta aactgcccac ttggcagtac atcaagtgta tcatatgcca agtacgcccc
      361 ctattgacgt caatgacggt aaatggcccg cctggcatta tgcccagtac atgaccttat
      421 gggactttcc tacttggcag tacatctacg tattagtcat cgctattacc atggtgatgc
      481 ggttttggca gtacatcaat gggcgtggat agcggtttga ctcacgggga tttccaagtc
      541 tccaccccat tgacgtcaat gggagtttgt tttggcacca aaatcaacgg gactttccaa
      601 aatgtcgtaa caactccgcc ccattgacgc aaatgggcgg taggcgtgta cggtgggagg
      661 tctatataag cagagctcgt ttagtgaacc gtcagatcgc ctggagacgc catccacgct
      721 gttttgacct ccatagaaga caccgggacc gatccagcct ccggactcta gaggatccct
      781 accggtgata tcctcgagac catggtgagc aagggcgagg agctgttcac cggggtggtg
      841 cccatcctgg tcgagctgga cggcgacgta aacggccaca agttcagcgt gtccggcgag
      901 ggcgagggcg atgccaccta cggcaagctg accctgaagt tcatctgcac caccggcaag
      961 ctgcccgtgc cctggcccac cctcgtgacc accttcacct acggcgtgca gtgcttcgcc
     1021 cgctaccccg accacatgaa gcagcacgac ttcttcaagt ccgccatgcc cgaaggctac
     1081 gtccaggagc gcaccatctt cttcaaggac gacggcaact acaagacccg cgccgaggtg
     1141 aagttcgagg gcgacaccct ggtgaaccgc atcgagctga agggcatcga cttcaaggag
     1201 gacggcaaca tcctggggca caagctggag tacaactaca acagccacaa ggtctatatc
     1261 accgccgaca agcagaagaa cggcatcaag gtgaacttca agacccgcca caacatcgag
     1321 gacggcagcg tgcagctcgc cgaccactac cagcagaaca cccccatcgg cgacggcccc
     1381 gtgctgctgc ccgacaacca ctacctgagc acccagtccg ccctgagcaa agaccccaac
     1441 gagaagcgcg atcacatggt cctgctggag ttcgtgaccg ccgccgggat cactctcggc
     1501 atggacgagc tgtacaaggg ctcgagccca tcaacaagtt tgtacaaaaa agcaggcttc
     1561 gaaggagata gaaccaattc tctaaggaaa tacttaacca tggtcatggt ggttctccgc
     1621 agcagcttgg agctgcacaa ccactccgcg gcctcggcca cgggctcctt ggacctgtcc
     1681 agtgacttcc tcagtctgga gcacatcggc cggaggcggc tccgctcggc cggcgcggcg
     1741 cagaagaaac ccgcggcgac cacagccaaa gcgggcgatg ggtcatcagt taaggaagtt
     1801 gaaacctacc accggacacg tgctttaaga tctttgagaa aagatgcaca gaattcttca
     1861 gattctagtt ttgagaagaa tgtggaaata acggagcaac ttgctaatgg caggcatttt
     1921 acaaggcagt tggccagaca gcaggctgat aaaaaaaaag aagagcacag agaagacaaa
     1981 gtgattccag ttactcggtc attgagggct agaaacatcg ttcaaagtac agaacactta
     2041 catgaagata atggtgatgt tgaagtgcgt cgaagttgta ggattagaag tcgttatagt
     2101 ggtgtaaacc agtccatgct gtttgacaaa cttataacta acactgctga agctgtactt
     2161 caaaaaatgg atgacatgaa gaagatgcgt agacagcgaa tgagagaact tgaagacttg
     2221 ggagtgttta atgaaacaga agaaagcaat cttaatatgt acacaagagg aaaacagaaa
     2281 gatattcaaa gaactgatga agaaacaact gataatcaag aaggcagtgt ggagtcatct
     2341 gaagagggtg aagaccaaga acatgaagat gatggtgaag atgaagatga tgaagatgat
     2401 gatgatgatg acgatgatga tgatgatgat gatgatgaag atgatgaaga tgaagaagat
     2461 ggagaagaag agaatcagaa gcgatattat cttagacaga gaaaagctac tgtttactat
     2521 caggctccat tggaaaaacc tcgtcaccag agaaagccca acatatttta tagtggccca
     2581 gcttctcctg caagaccaag ataccgatta tcttccgcag gaccaagaag tccttactgt
     2641 aaacgaatga acaggcgaag gcatgcaatc cacagtagtg actcgacttc atcttcctcc
     2701 tctgaagatg aacagcactt tgagaggcgg aggaaaagga gtcgtaatag ggctatcaat
     2761 aggtgcctcc cactaaattt tcggaaagat gaattaaaag gcatttataa agatcgaatg
     2821 aaaattggag caagccttgc cgatgttgat ccaatgcaac tagattcttc agtacgattt
     2881 gatagtgttg gtggcctgtc taatcatata gcagctctaa aagagatggt ggtgtttcca
     2941 ttactttatc cagaagtctt tgaaaaattt aaaattcaac ccccaagagg ttgtttgttt
     3001 tatgggccac ctggaactgg aaagactctg gttgccagag cacttgccaa tgagtgcagt
     3061 caaggggata aaagagtagc atttttcatg aggaaaggtg ctgattgtct aagtaaatgg
     3121 gtaggagaat ctgaaagaca gctacgattg ctgtttgatc aggcctatca gatgcgccca
     3181 tcaattattt tttttgacga aattgatggt ctggctccag tacggtcaag caggcaagat
     3241 cagattcaca gttctattgt ttccaccctg ctagctctta tggatggatt ggacagcaga
     3301 ggggaaattg tggtcattgg tgctacgaac aggctagatt ctatagatcc tgctttacga
     3361 aggcctggtc gctttgatag agaattcctc tttagcctgc ctgataaaga ggctcgaaaa
     3421 gagattctaa agattcacac cagggattgg aatcccaaac cactggacac atttttagaa
     3481 gagctagcag aaaactgtgt tggatactgt ggagcagata ttaaatcaat atgtgctgaa
     3541 gctgctttat gtgctttacg acgacgctac ccacagatct ataccactag tgagaaactg
     3601 cagttggatc tctcttcaat taatatctca gctaaggatt tcgaggtagc tatgcaaaag
     3661 atgataccag cctcccaaag agctgtgaca tcacctgggc aggcactgtc caccgttgtg
     3721 aaaccactcc tgcaaaacac tgttgacaag attttagaag ccctgcagag agtatttcca
     3781 catgcagaat tcagaacaaa taaaacatta gactcagata tttcttgtcc tctgctagaa
     3841 agtgacttgg cttacagtga tgatgatgtt ccatcagttt atgaaaatgg actttctcag
     3901 aaatcttctc ataaggcaaa agacaatttt aattttcttc atttgaatag aaatgcttgt
     3961 taccaaccta tgtcttttcg accaagaata ttgatagtag gagaaccagg atttgggcaa
     4021 ggttctcact tggcaccagc tgtcattcat gctttggaaa agtttactgt atatacatta
     4081 gacattcctg ttctttttgg agttagtact acatcccctg aagaaacatg tgcccaggtg
     4141 attcgtgaag ctaagagaac agcaccaagt atagtgtatg ttcctcatat ccacgtgtgg
     4201 tgggaaatag ttggaccgac acttaaagcc acatttacca cattattaca gaatattcct
     4261 tcatttgctc cagttttact acttgcaact tctgacaaac cccattccgc tttgccagaa
     4321 gaggtgcaag aattgtttat ccgtgattat ggagagattt ttaatgtcca gttaccggat
     4381 aaagaagaac ggacaaaatt ttttgaagat ttaattctaa aacaagctgc taagcctcct
     4441 atatcaaaaa agaaagcagt tttgcaggct ttggaggtac tcccagtagc accaccacct
     4501 gagccaagat cactgacagc agaagaagtg aaacgactag aagaacaaga agaagataca
     4561 tttagagaac tgaggatttt cttaagaaat gttacacata ggcttgctat tgacaagcga
     4621 ttccgagtgt ttactaagcc tgttgaccct gatgaggttc ctgattatgt cactgtaata
     4681 aagcaaccaa tggacctttc atctgtaatc agtaaaattg atctacacaa gtatctgact
     4741 gtgaaagact atttgagaga tattgatcta atctgtagta atgccttaga atacaatcca
     4801 gatagagatc ctggagatcg tcttattagg catagagcct gtgctttaag agatactgcc
     4861 tatgccataa ttaaagaaga acttgatgaa gactttgagc agctctgtga agaaattcag
     4921 gaatctagaa agaaaagagg ttgtagctcc tccaaatatg ccccgtctta ctaccatgtg
     4981 atgccaaagc aaaattccac tcttgttggt gataaaagat cagacccaga gcagaatgaa
     5041 aagctaaaga caccgagtac tcctgtggct tgcagcactc ctgctcagtt gaagaggaaa
     5101 attcgcaaaa agtcaaactg gtacttaggc accataaaaa agcgaaggaa gatttcacag
     5161 gcaaaggatg atagccagaa tgccatagat cacaaaattg agagtgatac agaggaaact
     5221 caagacacaa gtgtagatca taatgagacc ggaaacacag gagagtcttc ggtggaagaa
     5281 aatgaaaaac agcaaaatgc ctctgaaagc aaactggaat tgagaaataa ttcaaatact
     5341 tgtaatatag agaatgagct tgaagactct aggaagacta cagcatgtac agaattgaga
     5401 gacaagattg cttgtaatgg agatgcttct agctctcaga taatacatat ttctgatgaa
     5461 aatgaaggaa aagaaatgtg tgttctgcga atgactcgag ctagacgttc ccaggtagaa
     5521 cagcagcagc tcatcactgt tgaaaaggct ttggcaattc tttctcagcc tacaccctca
     5581 cttgttgtgg atcatgagcg attaaaaaat cttttgaaga ctgttgttaa aaaaagtcaa
     5641 aactacaaca tatttcagtt ggaaaatttg tatgcagtaa tcagccaatg tatttatcgg
     5701 catcgcaagg accatgataa aacatcactt attcagaaaa tggagcaaga ggtagaaaac
     5761 ttcagttgtt ccagattatc gcggccgcac tcgagatatc tagacccagc tttcttgtac
     5821 aaagtggttg ataacgggaa gcctatccct aaccctctcc tcggtctcga ttctacgcgt
     5881 accggttagt aatgagttta aacgggggag gctaactgaa acacggaagg agacaatacc
     5941 ggaaggaacc cgcgctatga cggcaataaa aagacagaat aaaacgcacg ggtgttgggt
     6001 cgtttgttca taaacgcggg gttcggtccc agggctggca ctctgtcgat accccaccga
     6061 gaccccattg gggccaatac gcccgcgttt cttccttttc cccaccccac cccccaagtt
     6121 cgggtgaagg cccagggctc gcagccaacg tcggggcggc aggccctgcc atagcagatc
     6181 tgcgcagctg gggctctagg gggtatcccc acgcgccctg tagcggcgca ttaagcgcgg
     6241 cgggtgtggt ggttacgcgc agcgtgaccg ctacacttgc cagcgcccta gcgcccgctc
     6301 ctttcgcttt cttcccttcc tttctcgcca cgttcgccgg ctttccccgt caagctctaa
     6361 atcgggggct ccctttaggg ttccgattta gtgctttacg gcacctcgac cccaaaaaac
     6421 ttgattaggg tgatggttca cgtagtgggc catcgccctg atagacggtt tttcgccctt
     6481 tgacgttgga gtccacgttc tttaatagtg gactcttgtt ccaaactgga acaacactca
     6541 accctatctc ggtctattct tttgatttat aagggatttt gccgatttcg gcctattggt
     6601 taaaaaatga gctgatttaa caaaaattta acgcgaatta attctgtgga atgtgtgtca
     6661 gttagggtgt ggaaagtccc caggctcccc agcaggcaga agtatgcaaa gcatgcatct
     6721 caattagtca gcaaccaggt gtggaaagtc cccaggctcc ccagcaggca gaagtatgca
     6781 aagcatgcat ctcaattagt cagcaaccat agtcccgccc ctaactccgc ccatcccgcc
     6841 cctaactccg cccagttccg cccattctcc gccccatggc tgactaattt tttttattta
     6901 tgcagaggcc gaggccgcct ctgcctctga gctattccag aagtagtgag gaggcttttt
     6961 tggaggccta ggcttttgca aaaagctccc gggagcttgt atatccattt tcggatctga
     7021 tcagcacgtg ttgacaatta atcatcggca tagtatatcg gcatagtata atacgacaag
     7081 gtgaggaact aaaccatggc caagcctttg tctcaagaag aatccaccct cattgaaaga
     7141 gcaacggcta caatcaacag catccccatc tctgaagact acagcgtcgc cagcgcagct
     7201 ctctctagcg acggccgcat cttcactggt gtcaatgtat atcattttac tgggggacct
     7261 tgtgcagaac tcgtggtgct gggcactgct gctgctgcgg cagctggcaa cctgacttgt
     7321 atcgtcgcga tcggaaatga gaacaggggc atcttgagcc cctgcggacg gtgccgacag
     7381 gtgcttctcg atctgcatcc tgggatcaaa gccatagtga aggacagtga tggacagccg
     7441 acggcagttg ggattcgtga attgctgccc tctggttatg tgtgggaggg ctaagcactt
     7501 cgtggccgag gagcaggact gacacgtgct acgagatttc gattccaccg ccgccttcta
     7561 tgaaaggttg ggcttcggaa tcgttttccg ggacgccggc tggatgatcc tccagcgcgg
     7621 ggatctcatg ctggagttct tcgcccaccc caacttgttt attgcagctt ataatggtta
     7681 caaataaagc aatagcatca caaatttcac aaataaagca tttttttcac tgcattctag
     7741 ttgtggtttg tccaaactca tcaatgtatc ttatcatgtc tgtataccgt cgacctctag
     7801 ctagagcttg gcgtaatcat ggtcatagct gtttcctgtg tgaaattgtt atccgctcac
     7861 aattccacac aacatacgag ccggaagcat aaagtgtaaa gcctggggtg cctaatgagt
     7921 gagctaactc acattaattg cgttgcgctc actgcccgct ttccagtcgg gaaacctgtc
     7981 gtgccagctg cattaatgaa tcggccaacg cgcggggaga ggcggtttgc gtattgggcg
     8041 ctcttccgct tcctcgctca ctgactcgct gcgctcggtc gttcggctgc ggcgagcggt
     8101 atcagctcac tcaaaggcgg taatacggtt atccacagaa tcaggggata acgcaggaaa
     8161 gaacatgtga gcaaaaggcc agcaaaaggc caggaaccgt aaaaaggccg cgttgctggc
     8221 gtttttccat aggctccgcc cccctgacga gcatcacaaa aatcgacgct caagtcagag
     8281 gtggcgaaac ccgacaggac tataaagata ccaggcgttt ccccctggaa gctccctcgt
     8341 gcgctctcct gttccgaccc tgccgcttac cggatacctg tccgcctttc tcccttcggg
     8401 aagcgtggcg ctttctcata gctcacgctg taggtatctc agttcggtgt aggtcgttcg
     8461 ctccaagctg ggctgtgtgc acgaaccccc cgttcagccc gaccgctgcg ccttatccgg
     8521 taactatcgt cttgagtcca acccggtaag acacgactta tcgccactgg cagcagccac
     8581 tggtaacagg attagcagag cgaggtatgt aggcggtgct acagagttct tgaagtggtg
     8641 gcctaactac ggctacacta gaagaacagt atttggtatc tgcgctctgc tgaagccagt
     8701 taccttcgga aaaagagttg gtagctcttg atccggcaaa caaaccaccg ctggtagcgg
     8761 tggttttttt gtttgcaagc agcagattac gcgcagaaaa aaaggatctc aagaagatcc
     8821 tttgatcttt tctacggggt ctgacgctca gtggaacgaa aactcacgtt aagggatttt
     8881 ggtcatgaga ttatcaaaaa ggatcttcac ctagatcctt ttaaattaaa aatgaagttt
     8941 taaatcaatc taaagtatat atgagtaaac ttggtctgac agttaccaat gcttaatcag
     9001 tgaggcacct atctcagcga tctgtctatt tcgttcatcc atagttgcct gactccccgt
     9061 cgtgtagata actacgatac gggagggctt accatctggc cccagtgctg caatgatacc
     9121 gcgagaccca cgctcaccgg ctccagattt atcagcaata aaccagccag ccggaagggc
     9181 cgagcgcaga agtggtcctg caactttatc cgcctccatc cagtctatta attgttgccg
     9241 ggaagctaga gtaagtagtt cgccagttaa tagtttgcgc aacgttgttg ccattgctac
     9301 aggcatcgtg gtgtcacgct cgtcgtttgg tatggcttca ttcagctccg gttcccaacg
     9361 atcaaggcga gttacatgat cccccatgtt gtgcaaaaaa gcggttagct ccttcggtcc
     9421 tccgatcgtt gtcagaagta agttggccgc agtgttatca ctcatggtta tggcagcact
     9481 gcataattct cttactgtca tgccatccgt aagatgcttt tctgtgactg gtgagtactc
     9541 aaccaagtca ttctgagaat agtgtatgcg gcgaccgagt tgctcttgcc cggcgtcaat
     9601 acgggataat accgcgccac atagcagaac tttaaaagtg ctcatcattg gaaaacgttc
     9661 ttcggggcga aaactctcaa ggatcttacc gctgttgaga tccagttcga tgtaacccac
     9721 tcgtgcaccc aactgatctt cagcatcttt tactttcacc agcgtttctg ggtgagcaaa
     9781 aacaggaagg caaaatgccg caaaaaaggg aataagggcg acacggaaat gttgaatact
     9841 catactcttc ctttttcaat attattgaag catttatcag ggttattgtc tcatgagcgg
     9901 atacatattt gaatgtattt agaaaaataa acaaataggg gttccgcgca catttccccg
     9961 aaaagtgcca cctgacgtcg acggatcggg agatctcccg atcccctatg gtgcactctc
    10021 agtacaatct gctctgatgc cgcatagtta agccagtatc tgctccctgc ttgtgtgttg
    10081 gaggtcgctg agtagtgcgc gagcaaaatt taagctacaa
//
